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    • 8. 发明专利
    • Polysaccharide structure and sequence determination
    • 多糖结构和序列测定
    • JP2010181411A
    • 2010-08-19
    • JP2010053792
    • 2010-03-10
    • Procognia (Israel) Ltdプロコグニア (イスラエル) リミテッド
    • MARKMAN OFER
    • G01N33/53C12M1/40C12Q1/34C12Q1/48G01N33/02G01N33/15G01N33/50G01N33/543G01N33/66
    • G01N33/66C12Q1/34G01N33/5308G01N2333/42G01N2333/924G01N2400/00G01N2400/02G01N2400/10Y10T436/143333
    • PROBLEM TO BE SOLVED: To provide a method for the structural analysis of a saccharide which overcomes all the problems associated with prior art methods. SOLUTION: The invention provides the method for the structural analysis of the saccharide which includes the following steps. The steps are: (a) the step of providing on the surface a plurality of essentially sequence- and/or site-specific binding agents; (b) the step of contacting the surface with a saccharide to be analyzed, or with a mixture including a plurality of fragments of the saccharide; (c) the step of washing or otherwise removing unbound saccharide or saccharide fragments; (d) the step of adding an essentially sequence- and/or site-specific markers, or a mixture of essentially sequence- and/or site-specific markers to the surface obtained in the step (c); (e) the step of acquiring one or more images of the markers that are bound to the surface; and (f) the step of deriving information related to the identity of the saccharide being analyzed from the images. COPYRIGHT: (C)2010,JPO&INPIT
    • 要解决的问题:提供克服所有与现有技术方法相关的问题的糖的结构分析方法。 解决方案:本发明提供了糖的结构分析方法,其包括以下步骤。 步骤是:(a)在表面上提供多个基本上序列和/或位点特异性结合剂的步骤; (b)使表面与要分析的糖接触的步骤,或与包含多个糖的片段的混合物接触; (c)洗涤或以其他方式去除未结合的糖或糖片段的步骤; (d)将基本上序列和/或位点特异性标记或基本上序列和/或位点特异性标记的混合物添加到步骤(c)中获得的表面的步骤; (e)获取绑定到表面的标记的一个或多个图像的步骤; 和(f)从图像中导出与被分析的糖的身份有关的信息的步骤。 版权所有(C)2010,JPO&INPIT