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1. 发明申请

WO2008002872A3 SOYBEAN EVENT 3560.4.3.5 AND COMPOSITIONS AND METHODS FOR THE IDENTIFICATION AND/OR DETECTION THEREOF 审中-公开
标题翻译：大豆事件3560.4.3.5及其鉴定和/或检测的组合物和方法
公开(公告)号：WO2008002872A3
公开(公告)日：2008-07-24
申请号：PCT/US2007072024
申请日：2007-06-25
申请人： PIONEER HI BRED INT , DU PONT , CRESSMAN ROBERT F , GUIDA ANTHONY D , HAZEL CHRISTINE B , HEGSTAD JEFFREY M , HENDERSON NANCY L , HONDRED DAVID , LEYSENS NANCY J , LI ZHONGSEN , VOGT MARK D , WEBER NATALIE N , XING AIQUI , ZHONG CATHY XIAOYAN
发明人： CRESSMAN ROBERT F , GUIDA ANTHONY D , HAZEL CHRISTINE B , HEGSTAD JEFFREY M , HENDERSON NANCY L , HONDRED DAVID , LEYSENS NANCY J , LI ZHONGSEN , VOGT MARK D , WEBER NATALIE N , XING AIQUI , ZHONG CATHY XIAOYAN
IPC分类号： C12N15/82 , A01H5/00 , C12Q1/68
CPC分类号： C12Q1/6895 , A01H5/10 , C12N15/8274 , C12N15/8275 , C12N15/8278
摘要： Compositions and methods related to transgenic glyphosate/ALS inhibitor-tolerant soybean plants are provided. Specifically, soybean plants having a 3560.4.3.5 event which imparts tolerance to glyphosate and at least one ALS-inhibiting herbicide are provided. The soybean plant harboring the 3560.4.3.5 event at the recited chromosomal location comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO: 10 and/or 11. The characterization of the genomic insertion site of the 3560.4.3.5 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean 3560.4.3.5 events are provided.
摘要翻译：提供了与转基因草甘膦/ ALS抑制剂耐受性大豆植物有关的组合物和方法。具体而言，提供了具有赋予草甘膦耐受性的3560.4.3.5事件的大豆植物和至少一种ALS抑制性除草剂。在所述染色体位置处携带3560.4.3.5事件的大豆植物包含至少具有SEQ ID NO：10和/或11的多核苷酸序列的基因组/转基因连接.3560.4.3.5事件的基因组插入位点的表征提供用于提高育种效率并且使得能够使用分子标记追踪育种群体及其后代中的转基因插入物。提供了用于鉴定，检测和使用大豆3560.4.3.5事件的各种方法和组合物。

2. 发明申请

WO2012129373A3 METHODS FOR PRODUCING A COMPLEX TRANSGENIC TRAIT LOCUS 审中-公开
标题翻译：用于生产复合转基因位置的方法
公开(公告)号：WO2012129373A3
公开(公告)日：2013-03-14
申请号：PCT/US2012030061
申请日：2012-03-22
申请人： PIONEER HI BRED INT , DU PONT , LASSNER MICHAEL , JANTZ DEREK , SMITH JAMES JEFFERSON , CIGAN MARK , FALCO CARL , GAO HUIRONG , LI ZHONGSEN , LIU ZHAN-BIN , SVITASHEV SERGEI
发明人： LASSNER MICHAEL , JANTZ DEREK , SMITH JAMES JEFFERSON , CIGAN MARK , FALCO CARL , GAO HUIRONG , LI ZHONGSEN , LIU ZHAN-BIN , SVITASHEV SERGEI
IPC分类号： C12N15/82 , A01H1/06 , A01H5/10 , C12N15/55
CPC分类号： C12N15/8202 , A01H1/06 , C12N9/22 , C12N15/8213
摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break- inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
摘要翻译：公开了在植物中生产复合转基因性状基因座的方法，所述基因座包含在目的基因组区域中的至少两个改变的靶序列。所述方法涉及使用两种或更多种双链断裂诱导剂，其中每一种可导致目的基因组区域中靶序列中的双链断裂，导致目标序列的改变。还公开了植物中的复杂的转基因性状基因座。复杂的转基因性状基因座包含与感兴趣的多核苷酸遗传连锁的至少两个改变的靶序列。还公开了植物，植物细胞，植物部分和包含一个或多个复合转基因性状基因座的种子。

3. 发明申请

WO2012071039A8 BRASSICA GAT EVENT DP-061061-7 AND COMPOSITIONS AND METHODS FOR THE IDENTIFICATION AND/OR DETECTION THEREOF 审中-公开
标题翻译： BRASSICA GAT EVENT DP-061061-7以及用于鉴定和/或检测它们的组合物和方法
公开(公告)号：WO2012071039A8
公开(公告)日：2012-08-23
申请号：PCT/US2010058002
申请日：2010-11-24
申请人： PIONEER HI BRED INT , DU PONT , TULSIERAM LOMAS , ZHANG YONGPING , PATEL JAYANTILAL DEVABHAI , CHARNE DAVID GEORGE , CHEN WENPIN , THOONEN FERDINAND GERARD , KOSCIELNY CHADWICK BRUCE , LI ZHONGSEN
发明人： TULSIERAM LOMAS , ZHANG YONGPING , PATEL JAYANTILAL DEVABHAI , CHARNE DAVID GEORGE , CHEN WENPIN , THOONEN FERDINAND GERARD , KOSCIELNY CHADWICK BRUCE , LI ZHONGSEN
IPC分类号： C12N15/82
CPC分类号： C12N15/8275 , C12N15/8274
摘要： Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-061061-7 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-061061-7 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of events provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the events are provided.
摘要翻译：提供了与转基因抗草甘膦芸苔属植物有关的组合物和方法。具体而言，本发明提供了具有赋予草甘膦耐受性的DP-061061-7事件的芸苔属植物。在所述染色体位置处携带DP-061061-7事件的芸苔属植物包含SEQ ID NO：2内的基因组/转基因连接或包含SEQ ID NO：12和/或13所示的基因组/转基因连接。事件的基因组插入位点提供了增强的育种效率并且使得能够使用分子标记追踪育种群体中的转基因插入物及其后代。提供了用于识别，检测和使用事件的各种方法和组合物。

4. 发明专利

UA123532C2 СПОСІБ ІДЕНТИФІКАЦІЇ ВАРІАНТНОГО САЙТА РОЗПІЗНАВАННЯ ДЛЯ СКОНСТРУЙОВАНОГО ЗАСОБУ, ЩО РІДКО РОЗЩЕПЛЮЄ, ДЛЯ ІНДУКЦІЇ ДВОНИТКОВОГО РОЗРИВУ 未知
公开(公告)号：UA123532C2
公开(公告)日：2021-04-21
申请号：UAA201509935
申请日：2014-03-10
申请人： DU PONT , PIONEER HI BRED INT
发明人： DESCHAMPS STEPHANE , ENGLISH JAMES , LI ZHONGSEN , LLACA VICTOR , YOUNG JOSHUA K
摘要： Винахідстосуєтьсяспособуідентифікаціїваріантногосайтарозпізнаваннядлясконструйованогозасобу, щорідкорозщеплює, дляіндукціїдвонитковогорозриву, здатногодовведеннярідкогодвонитковогорозривув припустимийсайтрозпізнавання, причомузазначенийспосібвключає (a) приведенняв контактгеномноїДНКізсконструйованимзасобом, щорідкорозщеплює, дляіндукціїдвонитковогорозриву, здатнимдовведеннядвонитковогорозривув розпізнаваннязазначенугеномуДНК, дедвонитковийрозривприводитьу результатідоутвореннянуклеотидноголипкогокінця; (b) лігуванняпершогоадаптераіззазначенимнуклеотиднимлипкимкінцем; (c) розрізаннялігованоїДНК, отриманоїнастадії (b), ілігуваннящонайменшеодногодругогоадаптераз розрізанимнуклеотиднимкінцемдлязабезпеченняампліфікаціїтасеквенуванняфрагментівгеномноїДНК, щооточуютьдвонитковийрозрив; (d) вирівнюваннянуклеотиднихпослідовностейфрагментівДНК, отриманихв (с), зеталонноюгеномноюпослідовністюДНК, та (e) ідентифікаціюваріантногосайтарозпізнавання, щоміститьзмінущонайменшепооднійнуклеотиднійосновів порівнянніз припустимимсайтомрозпізнаваннязазначеногосконструйованогозасобудляіндукціїдвонитковогорозриву. Винахідтакожстосуєтьсяспособуідентифікаціїваріантногосайтарозпізнаванняз покращеноюактивністюрозщепленнядлясконструйованогозасобу, щорідкорозщеплює, дляіндукціїдвонитковогорозриву, здатногодовведеннярідкогодвонитковогорозривув припустимийсайтрозпізнавання.

5. 发明专利

AU2014308896A1 Plant genome modification using guide RNA/Cas endonuclease systems and methods of use 未知
公开(公告)号：AU2014308896A1
公开(公告)日：2016-03-10
申请号：AU2014308896
申请日：2014-08-20
申请人： DU PONT , PIONEER HI BRED INT
发明人： CIGAN ANDREW , FALCO SAVERIO CARL , GAO HUIRONG , LI ZHONGSEN , LIU ZHAN-BIN , LYZNIK L ALEKSANDER , SHI JINRUI , SVITASHEV SERGEI , YOUNG JOSHUA K
IPC分类号： C12N15/82
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.

6. 发明专利

AU2015268681A1 BRASSICA GAT EVENT DP-073496-4 AND COMPOSITIONS AND METHODS FOR THE IDENTIFICATION AND/OR DETECTION THEREOF 未知
公开(公告)号：AU2015268681A1
公开(公告)日：2016-01-07
申请号：AU2015268681
申请日：2015-12-11
申请人： PIONEER HI BRED INT , DU PONT
发明人： CHARNE DAVID GEORGE , CHEN WENPIN , KOSCIELNY CHADWICK BRUCE , PATEL JAYANTILAL DEVABHAI , THOONEN FERDINAND GERARD , TULSIERAM LOMAS , ZHANG YONGPING , LI ZHONGSEN
IPC分类号： C12N15/82
摘要： Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants 5 having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of 10 molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided. Z.0 0 m znw ozi n- 0 IxG -L 0u CC toE 0) It0p a. wL LiiT

7. 发明专利

CA2922046A1 A SOYBEAN U6 POLYMERASE III PROMOTER AND METHODS OF USE 未知
公开(公告)号：CA2922046A1
公开(公告)日：2015-02-26
申请号：CA2922046
申请日：2014-08-20
申请人： DU PONT , PIONEER HI BRED INT
发明人： CIGAN ANDREW MARK , FALCO SAVERIO CARL , LI ZHONGSEN , LIU ZHAN-BIN , YOUNG JOSHUA K
IPC分类号： C12N15/82 , A01H5/00 , C12N5/10 , C12N15/113
摘要： The disclosure relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

8. 发明专利

AU2010364322A1 Brassica GAT event DP-073496-4 and compositions and methods for the identification and/or detection thereof 未知
公开(公告)号：AU2010364322A1
公开(公告)日：2013-02-28
申请号：AU2010364322
申请日：2010-11-24
申请人： PIONEER HI BRED INT , E I DU PONT DE NEMORS AND COMPANY
发明人： CHARNE DAVID GEORGE , CHEN WENPIN , KOSCIELNY CHADWICK BRUCE , PATEL JAYANTILAL DEVABHAI , THOONEN FERDINAND GERARD , TULSIERAM LOMAS , ZHANG YONGPING , LI ZHONGSEN
IPC分类号： C12N15/82 , A01H1/00 , A01H5/00 , A01H5/10 , A01H6/20 , C12Q1/6895
摘要： Compositions and methods related to transgenic glyphosate tolerant

10. 发明专利

CA2891153C BRASSICA GAT EVENT DP-073496-4 AND COMPOSITIONS AND METHODS FOR THE IDENTIFICATION AND/OR DETECTION THEREOF 未知
公开(公告)号：CA2891153C
公开(公告)日：2018-04-03
申请号：CA2891153
申请日：2010-11-24
申请人： PIONEER HI BRED INT , DU PONT
发明人： CHARNE DAVID GEORGE , CHEN WENPIN , KOSCIELNY CHADWICK BRUCE , PATEL JAYANTILAL DEVABHAI , THOONEN FERDINAND GERARD , TULSIERAM LOMAS , ZHANG YONGPING , LI ZHONGSEN
IPC分类号： C12N5/10 , A01H1/00 , A01H1/04 , A01H5/00 , A01N25/32 , A01N57/20 , A01P13/00 , C12N5/04 , C12N15/54 , C12N15/82 , C12Q1/6895
摘要： Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP- 073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

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