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1. 发明申请

WO2008112019A2 MAIZE EVENT DP-098140-6 AND COMPOSITIONS AND METHODS FOR THE IDENTIFICATION AND/OR DETECTION THEREOF 审中-公开
标题翻译：玉米事件DP-098140-6及其鉴定和/或检测的组合物和方法
公开(公告)号：WO2008112019A2
公开(公告)日：2008-09-18
申请号：PCT/US2007081468
申请日：2007-10-16
申请人： PIONEER HI BRED INT , DU PONT , CHICOINE TIMOTHY K , DERRY JEFFREY W , HANZEL CHRISTINE B , HENDERSON NANCY L , HONDRED DAVID , KHAZI DEEPA , LEYSENS NANCY J , LIU DONGLONG , MCCUTCHEIN BILLY FRED , MEHRE WAYNE , PEEPLES KENNETH A , SAUNDERS DAVID W , WEBER NATALIE N , YOUNG JOSHUA K , ZHONG GAN-YUAN , ZHONG CATHY XIAOYAN
发明人： CHICOINE TIMOTHY K , DERRY JEFFREY W , HANZEL CHRISTINE B , HENDERSON NANCY L , HONDRED DAVID , KHAZI DEEPA , LEYSENS NANCY J , LIU DONGLONG , MCCUTCHEIN BILLY FRED , MEHRE WAYNE , PEEPLES KENNETH A , SAUNDERS DAVID W , WEBER NATALIE N , YOUNG JOSHUA K , ZHONG GAN-YUAN , ZHONG CATHY XIAOYAN
IPC分类号： C12N15/82
CPC分类号： C12N15/8275
摘要： Compositions and methods related to transgenic glyphosate/ALS inhibitor- tolerant maize plants are provided. Specifically, the present invention provides maize plants having a DP-098140-6 event which imparts tolerance to glyphosate and at least one ALS-inhibiting herbicide. The maize plant harboring the DP-098140-6 event at the recited chromosomal location comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO: 5 and/or 6. The characterization of the genomic insertion site of the DP-098140-6 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the trans gene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the maize DP-098140-6 events are provided.
摘要翻译：提供了与转基因草甘膦/ ALS抑制剂耐受玉米植物有关的组合物和方法。具体而言，本发明提供了具有赋予对草甘膦和至少一种抑制ALS的除草剂的耐受性的DP-098140-6事件的玉米植物。在所述染色体位置处携带DP-098140-6事件的玉米植物包含至少具有SEQ ID NO：5和/或6的多核苷酸序列的基因组/转基因连接.DP-098140的基因组插入位点的表征 -6事件提供了增强的育种效率并且使得能够使用分子标记追踪育种群体及其后代中的转基因插入物。提供了用于鉴定，检测和使用玉米DP-098140-6事件的各种方法和组合物。

2. 发明申请

WO2008112019A8 MAIZE EVENT DP-098140-6 AND COMPOSITIONS AND METHODS FOR THE IDENTIFICATION AND/OR DETECTION THEREOF 审中-公开
标题翻译：主要事项DP-098140-6及其鉴定和/或检测的组合物和方法
公开(公告)号：WO2008112019A8
公开(公告)日：2009-03-12
申请号：PCT/US2007081468
申请日：2007-10-16
申请人： PIONEER HI BRED INT , DU PONT , CHICOINE TIMOTHY K , DERRY JEFFREY W , HAZEL CHRISTINE B , HENDERSON NANCY L , HONDRED DAVID , KHAZI DEEPA , LEYSENS NANCY J , LIU DONGLONG , MCCUTCHEIN BILLY FRED , MEHRE WAYNE , PEEPLES KENNETH A , SAUNDERS DAVID W , WEBER NATALIE N , YOUNG JOSHUA K , ZHONG GAN-YUAN , ZHONG CATHY XIAOYAN
发明人： CHICOINE TIMOTHY K , DERRY JEFFREY W , HAZEL CHRISTINE B , HENDERSON NANCY L , HONDRED DAVID , KHAZI DEEPA , LEYSENS NANCY J , LIU DONGLONG , MCCUTCHEIN BILLY FRED , MEHRE WAYNE , PEEPLES KENNETH A , SAUNDERS DAVID W , WEBER NATALIE N , YOUNG JOSHUA K , ZHONG GAN-YUAN , ZHONG CATHY XIAOYAN
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8275
摘要： Compositions and methods related to transgenic glyphosate/ALS inhibitor- tolerant maize plants are provided. Specifically, the present invention provides maize plants having a DP-098140-6 event which imparts tolerance to glyphosate and at least one ALS-inhibiting herbicide. The maize plant harboring the DP-098140-6 event at the recited chromosomal location comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO: 5 and/or 6. The characterization of the genomic insertion site of the DP-098140-6 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the trans gene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the maize DP-098140-6 events are provided.
摘要翻译：提供了与转基因草甘膦/ ALS抑制剂耐受性玉米植物相关的组合物和方法。具体地说，本发明提供具有DP-098140-6事件的玉米植物，其赋予对草甘膦和至少一种ALS抑制性除草剂的耐受性。携带所述染色体位置的DP-098140-6事件的玉米植物包含至少具有SEQ ID NO：5和/或6的多核苷酸序列的基因组/转基因接头。DP-098140的基因组插入位点的表征 -6事件提供增强的育种效率并且能够使用分子标记来跟踪繁殖种群及其后代中的转基因插入物。提供了用于识别，检测和使用玉米DP-098140-6事件的各种方法和组合物。

3. 发明申请

WO2009103049A3 PLANT GENOMIC DNA FLANKING SPT EVENT AND METHODS FOR IDENTIFYING SPT EVENT 审中-公开
标题翻译：植物基因组DNA片段SPT事件和鉴定SPT事件的方法
公开(公告)号：WO2009103049A3
公开(公告)日：2009-12-03
申请号：PCT/US2009034212
申请日：2009-02-16
申请人： PIONEER HI BRED INT , DU PONT , BRINK KENT , CROWGEY ERIN , DIETRICH NINA , HONDRED DAVID , YOUNG JOSHUA K , ZHONG CATHY XIAOYAN
发明人： BRINK KENT , CROWGEY ERIN , DIETRICH NINA , HONDRED DAVID , YOUNG JOSHUA K , ZHONG CATHY XIAOYAN
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： A01H5/00 , C12N15/8245 , C12N15/8289
摘要： Compositions and methods related to transgenic plants comprising seed production technology are provided. Specifically, maize plants having a E6611.32.1.38 event which confers seed production technology are provided. The plant harboring the E6611.32.1.38 event at the recited chromosomal location comprises the genomic/transgene junctions described. The plant genomic DNA flanking the integrated E6611.32.1.38 event can be used to design assays that will be specific for the E6611.32.1.38 event. The characterization of the genomic insertion site of the E6611.32.1.38 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the maize E6611.32.1.38 event are provided.
摘要翻译：提供了与包含种子生产技术的转基因植物有关的组合物和方法。具体而言，提供了具有赋予种子生产技术的E6611.32.1.38事件的玉米植物。在所述染色体位置处携带E6611.32.1.38事件的植物包含所述的基因组/转基因连接。整合E6611.32.1.38事件侧翼的植物基因组DNA可用于设计对E6611.32.1.38事件特异的测定。 E6611.32.1.38事件的基因组插入位点的表征提供了增强的育种效率并且使得能够使用分子标记追踪育种群体中的转基因插入物及其后代。提供了用于鉴定，检测和使用玉米E6611.32.1.38事件的各种方法和组合物。

4. 发明专利

AU2014308900A1 A soybean U6 polymerase III promoter and methods of use 未知
公开(公告)号：AU2014308900A1
公开(公告)日：2016-03-10
申请号：AU2014308900
申请日：2014-08-20
申请人： DU PONT , PIONEER HI BRED INT
发明人： CIGAN ANDREW MARK , FALCO SAVERIO CARL , LI ZHONGSEN , LIU ZHAN-BIN , YOUNG JOSHUA K
IPC分类号： C12N15/82 , C07K14/415
摘要： The disclosure relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

5. 发明专利

CA2872096A1 COMPOSITIONS AND METHODS COMPRISING SEQUENCES HAVING MEGANUCLEASE ACTIVITY 未知
公开(公告)号：CA2872096A1
公开(公告)日：2013-11-07
申请号：CA2872096
申请日：2013-05-01
申请人： DU PONT , PIONEER HI BRED INT
发明人： BERMUDEZ ERICKA , CIGAN ANDREW MARK , ENGLISH JAMES , FALCO SAVERIO CARL , GAO HUIRONG , LIU LU , LIU ZHAN-BIN , ONG AZALEA , SVITASHEV SERGEI , YOUNG JOSHUA K
IPC分类号： C12N9/22 , C12N15/82
摘要： Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.

6. 发明专利

CA2871557A1 MAIZE EVENT DP-004114-3 AND METHODS FOR DETECTION THEREOF 未知
公开(公告)号：CA2871557A1
公开(公告)日：2013-10-03
申请号：CA2871557
申请日：2013-03-28
申请人： PIONEER HI BRED INT , DU PONT
发明人： ALARCON CLARA M , HARMON MATTHEW C , PASCUAL MARIA ALEJANDRA , REGISTER JAMES C III , SCELONGE CHRISTOPHER J , YOUNG JOSHUA K , ZHONG CATHY XIAOYAN
IPC分类号： C12N15/82 , C12Q1/68
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

7. 发明专利

UA123532C2 СПОСІБ ІДЕНТИФІКАЦІЇ ВАРІАНТНОГО САЙТА РОЗПІЗНАВАННЯ ДЛЯ СКОНСТРУЙОВАНОГО ЗАСОБУ, ЩО РІДКО РОЗЩЕПЛЮЄ, ДЛЯ ІНДУКЦІЇ ДВОНИТКОВОГО РОЗРИВУ 未知
公开(公告)号：UA123532C2
公开(公告)日：2021-04-21
申请号：UAA201509935
申请日：2014-03-10
申请人： DU PONT , PIONEER HI BRED INT
发明人： DESCHAMPS STEPHANE , ENGLISH JAMES , LI ZHONGSEN , LLACA VICTOR , YOUNG JOSHUA K
摘要： Винахідстосуєтьсяспособуідентифікаціїваріантногосайтарозпізнаваннядлясконструйованогозасобу, щорідкорозщеплює, дляіндукціїдвонитковогорозриву, здатногодовведеннярідкогодвонитковогорозривув припустимийсайтрозпізнавання, причомузазначенийспосібвключає (a) приведенняв контактгеномноїДНКізсконструйованимзасобом, щорідкорозщеплює, дляіндукціїдвонитковогорозриву, здатнимдовведеннядвонитковогорозривув розпізнаваннязазначенугеномуДНК, дедвонитковийрозривприводитьу результатідоутвореннянуклеотидноголипкогокінця; (b) лігуванняпершогоадаптераіззазначенимнуклеотиднимлипкимкінцем; (c) розрізаннялігованоїДНК, отриманоїнастадії (b), ілігуваннящонайменшеодногодругогоадаптераз розрізанимнуклеотиднимкінцемдлязабезпеченняампліфікаціїтасеквенуванняфрагментівгеномноїДНК, щооточуютьдвонитковийрозрив; (d) вирівнюваннянуклеотиднихпослідовностейфрагментівДНК, отриманихв (с), зеталонноюгеномноюпослідовністюДНК, та (e) ідентифікаціюваріантногосайтарозпізнавання, щоміститьзмінущонайменшепооднійнуклеотиднійосновів порівнянніз припустимимсайтомрозпізнаваннязазначеногосконструйованогозасобудляіндукціїдвонитковогорозриву. Винахідтакожстосуєтьсяспособуідентифікаціїваріантногосайтарозпізнаванняз покращеноюактивністюрозщепленнядлясконструйованогозасобу, щорідкорозщеплює, дляіндукціїдвонитковогорозриву, здатногодовведеннярідкогодвонитковогорозривув припустимийсайтрозпізнавання.

8. 发明专利

ES2816640T3 Evento de maíz DP-004114-3 y métodos para la detección del mismo 未知
公开(公告)号：ES2816640T3
公开(公告)日：2021-04-05
申请号：ES13715578
申请日：2013-03-28
申请人： PIONEER HI BRED INT , DU PONT
发明人： ALARCON CLARA M , HARMON MATTHEW C , PASCUAL MARIA ALEJANDRA , REGISTER JAMES C , SCELONGE CHRISTOPHER J , YOUNG JOSHUA K , ZHONG CATHY XIAOYAN
IPC分类号： C12N15/82 , C12Q1/68
摘要： Un amplicón que consiste en la secuencia de ácido nucleico de la SEQ ID NO: 32 o el complemento de longitud completa del mismo.

9. 发明专利

AU2014308896A1 Plant genome modification using guide RNA/Cas endonuclease systems and methods of use 未知
公开(公告)号：AU2014308896A1
公开(公告)日：2016-03-10
申请号：AU2014308896
申请日：2014-08-20
申请人： DU PONT , PIONEER HI BRED INT
发明人： CIGAN ANDREW , FALCO SAVERIO CARL , GAO HUIRONG , LI ZHONGSEN , LIU ZHAN-BIN , LYZNIK L ALEKSANDER , SHI JINRUI , SVITASHEV SERGEI , YOUNG JOSHUA K
IPC分类号： C12N15/82
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.

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