专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2012129373A3 METHODS FOR PRODUCING A COMPLEX TRANSGENIC TRAIT LOCUS 审中-公开
标题翻译：用于生产复合转基因位置的方法
公开(公告)号：WO2012129373A3
公开(公告)日：2013-03-14
申请号：PCT/US2012030061
申请日：2012-03-22
申请人： PIONEER HI BRED INT , DU PONT , LASSNER MICHAEL , JANTZ DEREK , SMITH JAMES JEFFERSON , CIGAN MARK , FALCO CARL , GAO HUIRONG , LI ZHONGSEN , LIU ZHAN-BIN , SVITASHEV SERGEI
发明人： LASSNER MICHAEL , JANTZ DEREK , SMITH JAMES JEFFERSON , CIGAN MARK , FALCO CARL , GAO HUIRONG , LI ZHONGSEN , LIU ZHAN-BIN , SVITASHEV SERGEI
IPC分类号： C12N15/82 , A01H1/06 , A01H5/10 , C12N15/55
CPC分类号： C12N15/8202 , A01H1/06 , C12N9/22 , C12N15/8213
摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break- inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
摘要翻译：公开了在植物中生产复合转基因性状基因座的方法，所述基因座包含在目的基因组区域中的至少两个改变的靶序列。所述方法涉及使用两种或更多种双链断裂诱导剂，其中每一种可导致目的基因组区域中靶序列中的双链断裂，导致目标序列的改变。还公开了植物中的复杂的转基因性状基因座。复杂的转基因性状基因座包含与感兴趣的多核苷酸遗传连锁的至少两个改变的靶序列。还公开了植物，植物细胞，植物部分和包含一个或多个复合转基因性状基因座的种子。

2. 发明申请

WO2010062518A4 SULFONYLUREA-RESPONSIVE REPRESSOR PROTEINS 审中-公开
标题翻译：磺酰脲类响应性抑制剂蛋白质
公开(公告)号：WO2010062518A4
公开(公告)日：2010-08-05
申请号：PCT/US2009061661
申请日：2009-10-22
申请人： PIONEER HI BRED INT , DU PONT , LASSNER MICHAEL , LOOGER LOREN L , MCBRIDE KEVIN E , MCGONIGLE BRIAN
发明人： LASSNER MICHAEL , LOOGER LOREN L , MCBRIDE KEVIN E , MCGONIGLE BRIAN
IPC分类号： C07K14/195
CPC分类号： C07K14/245 , C07K14/195 , C12N15/635 , C12N15/8217 , C12N15/8238 , C12N15/8278
摘要： Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.
摘要翻译：提供了与使用磺酰脲类应答抑制剂有关的组合物和方法。组合物包括与操纵子特异性结合的多肽，其中特异性结合受磺酰脲类化合物调节。组合物还包括编码多肽的多核苷酸以及构建体，载体，原核和真核细胞以及真核生物，包括包含该多核苷酸的植物和种子，和/或通过所述方法产生。还提供了向细胞或生物体提供磺酰脲类反应性阻遏物并调节目的多核苷酸在细胞或生物体（包括植物或植物细胞）中的表达的方法。

3. 发明申请

WO2009091864A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LYGUS 审中-公开
标题翻译：抑制淋巴瘤靶向多核苷酸的组合物和方法
公开(公告)号：WO2009091864A3
公开(公告)日：2009-12-10
申请号：PCT/US2009031057
申请日：2009-01-15
申请人： PIONEER HI BRED INT , DU PONT , HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
发明人： HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , C12N15/113 , C12N15/8218 , C12N2310/141 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, and further provides target polynucleotides set forth in SEQ ID NOS:1-21 or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Lygus Hesperus. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉默元件的方法和组合物，所述沉默元件当被有害生物如来自草盲蝽属的害虫摄入时，能够降低害虫中靶序列的表达。在具体的实施方案中，靶序列表达的降低控制害虫，并且由此该方法和组合物能够限制对植物的损害。本发明提供了来自本文公开的特定多肽家族的各种靶多核苷酸，并且还提供了SEQ ID NO：1-21中列出的靶多核苷酸或其活性变体，其中靶害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供了沉默元件，其在被害虫摄入时降低靶多肽的水平并由此控制害虫。在具体的实施方案中，害虫是草盲蝽（Lygus Hesperus）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其他宿主细胞。

4. 发明申请

WO2009091860A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM THE FAMILY APHIDIDAE 审中-公开
标题翻译：用于抑制来自家庭蚜虫目标多核苷酸的组合物和方法
公开(公告)号：WO2009091860A3
公开(公告)日：2009-11-05
申请号：PCT/US2009031053
申请日：2009-01-15
申请人： PIONEER HI BRED INT , DU PONT , HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
发明人： HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C07K14/43563 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉默元件的方法和组合物，所述沉默元件当被有害生物例如来自蚜科的害虫摄取时，能够降低害虫中靶序列的表达。在具体的实施方案中，靶序列表达的降低控制害虫，并且由此该方法和组合物能够限制对植物的损害。本发明提供了特定蛋白质类别的靶多核苷酸以及如SEQ ID NO：1,2,3,4,5,6,7,8,9,10或其活性变体或片段所示的靶多核苷酸，其中a 目标害虫中一个或多个序列表达的减少控制了害虫（即，具有杀虫活性）。进一步提供了沉默元件，其在被害虫摄入时降低靶多肽的水平并由此控制害虫。在具体的实施方案中，害虫是蚜虫（Aphis）甘氨酸。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其他宿主细胞。

5. 发明申请

WO2009091862A2 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA 审中-公开
标题翻译：用于抑制来自LEPIDOPTERA的目标多核苷酸的组合物和方法
公开(公告)号：WO2009091862A2
公开(公告)日：2009-07-23
申请号：PCT/US2009031055
申请日：2009-01-15
申请人： PIONEER HI BRED INT , DU PONT , HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
发明人： HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N65/20 , A01N65/44 , C12N15/113 , C12N2310/11 , C12N2310/14 , C12N2310/531 , C12N2320/32 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当沉积元件在被害虫如来自鳞翅目序列的害虫中摄取时，它们能够降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供编码来自特定蛋白质家族的多肽的多核苷酸和SEQ ID NO：1,2,3,4,5,6,7,8,9,10,11,12,13,14中所示的各种靶多核苷酸的靶多核苷酸，15，16，17，18，19，20，21，22，23，24，25，26，27，28，29，30，31，32，33，34，35，36，37，38，39 ，40,41,42,43,44,45,46,47,48,49或50个或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即，具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是草地夜蛾（Spodoptera frugiperda）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

6. 发明申请

WO2006091219A3 METHODS OF PROTECTING PLANTS FROM PATHOGENIC FUNGI 审中-公开
标题翻译：从致病真菌保护植物的方法
公开(公告)号：WO2006091219A3
公开(公告)日：2007-05-31
申请号：PCT/US2005023327
申请日：2005-06-30
申请人： PIONEER HI BRED INT , LASSNER MICHAEL , WU GUSUI , MULLER MATHIAS L , KEENAN ROBERT J , ALI HANA
发明人： LASSNER MICHAEL , WU GUSUI , MULLER MATHIAS L , KEENAN ROBERT J , ALI HANA
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8282
摘要： Methods for protecting a plant from a plant pathogenic fungus are provided. A method for enhancing fungal pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antifungal polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antifungal polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了保护植物免受植物病原真菌侵害的方法。进一步提供了使用本文公开的核苷酸序列增强植物中真菌病原体抗性的方法。该方法包括向植物中引入表达盒，所述表达盒包含与编码本发明抗真菌多肽的核苷酸序列可操作连接的启动子。还公开了包含编码实施方案的抗真菌多肽或其变体或片段的核苷酸序列的转化植物，植物细胞，种子和微生物。

7. 发明申请

WO2006085966A9 METHODS OF PROTECTING PLANTS FROM PATHOGENIC FUNGI AND NEMATODES 审中-公开
标题翻译：从致病真菌和线虫保护植物的方法
公开(公告)号：WO2006085966A9
公开(公告)日：2006-09-21
申请号：PCT/US2005023325
申请日：2005-06-30
申请人： PIONEER HI BRED INT , LASSNER MICHAEL , WU GUSUI , MULLER MATHIAS L , KEENAN ROBERT J , ALI HANA , SHAH GOWRI , WEI JUN-ZHI
发明人： LASSNER MICHAEL , WU GUSUI , MULLER MATHIAS L , KEENAN ROBERT J , ALI HANA , SHAH GOWRI , WEI JUN-ZHI
CPC分类号： C12N15/8285 , C07K14/415 , C12N15/8282 , Y02A40/164
摘要： Methods for protecting a plant from a pathogen, particularly a pathogenic fungus or nematode, are provided. A method for enhancing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了保护植物免受病原体，特别是致病真菌或线虫的方法。进一步提供了使用本文公开的核苷酸序列增强植物中病原体抗性的方法。该方法包括向植物中引入表达盒，所述表达盒包含与编码本发明的抗病原菌多肽的核苷酸序列可操作连接的启动子。还公开了包含编码实施方案的抗病原体多肽或其变体或片段的核苷酸序列的转化植物，植物细胞，种子和微生物。

8. 发明申请

WO2005123929A3 PLASTID TRANSIT PEPTIDES 审中-公开
标题翻译：塑料过滤胶
公开(公告)号：WO2005123929A3
公开(公告)日：2006-03-30
申请号：PCT/US2005020455
申请日：2005-06-09
申请人： PIONEER HI BRED INT , LASSNER MICHAEL , WILKINSON JACK Q
发明人： LASSNER MICHAEL , WILKINSON JACK Q
IPC分类号： A01H1/00 , C07H21/04 , C07K14/00 , C07K14/415 , C12N5/04 , C12N9/88 , C12N15/82 , C12Q1/68
CPC分类号： C07K14/415 , C07K14/00 , C12N15/8221
摘要： The present invention provides novel plastid transit peptides that direct lacalization of attached moieties (e.g., polypeptides) into plant plastids. The present invension also relates to methods and compositions for lacalizing polypeptides to plant plastids including, but not limited to, transgenic plant production.
摘要翻译：本发明提供了将附着部分（例如，多肽）引导到植物质体中的新型质体转运肽。本发明还涉及用于将多肽拉染植物质体的方法和组合物，包括但不限于转基因植物生产。

9. 发明申请

WO2006085965A3 METHODS OF PROTECTING PLANTS FROM PATHOGENIC FUNGI 审中-公开
标题翻译：从病原菌保护植物的方法
公开(公告)号：WO2006085965A3
公开(公告)日：2007-06-21
申请号：PCT/US2005023319
申请日：2005-06-30
申请人： PIONEER HI BRED INT , LASSNER MICHAEL , WU GUSUI , MULLER MATHIAS L , KEENAN ROBERT J , ALI HANA
发明人： LASSNER MICHAEL , WU GUSUI , MULLER MATHIAS L , KEENAN ROBERT J , ALI HANA
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8282
摘要： Methods for protecting a plant from a plant pathogenic fungus are provided. A method for enhancing fungal pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antifungal polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antifungal polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了保护植物免受植物致病真菌的方法。还提供了使用本文公开的核苷酸序列增强植物中真菌病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗真菌多肽的核苷酸序列的启动子的表达盒。还公开了包含编码实施方案的抗真菌多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

10. 发明申请

WO2006085966A3 METHODS OF PROTECTING PLANTS FROM PATHOGENIC FUNGI AND NEMATODES 审中-公开
标题翻译：从病原真菌和NEMATODES保护植物的方法
公开(公告)号：WO2006085966A3
公开(公告)日：2007-06-07
申请号：PCT/US2005023325
申请日：2005-06-30
申请人： PIONEER HI BRED INT , LASSNER MICHAEL , WU GUSUI , MULLER MATHIAS L , KEENAN ROBERT J , ALI HANA , SHAH GOWRI , WEI JUN-ZHI
发明人： LASSNER MICHAEL , WU GUSUI , MULLER MATHIAS L , KEENAN ROBERT J , ALI HANA , SHAH GOWRI , WEI JUN-ZHI
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8285 , C07K14/415 , C12N15/8282 , Y02A40/164
摘要： Methods for protecting a plant from a pathogen, particularly a pathogenic fungus or nematode, are provided. A method for enhancing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the embodiments, or variant or fragment thereof, are also disclosed.
摘要翻译：提供了保护植物免受病原体，特别是病原真菌或线虫的方法。还提供了使用本文公开的核苷酸序列增强植物中病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还公开了包含编码实施方案的抗病原体多肽的核苷酸序列或其变体或片段的转化植物，植物细胞，种子和微生物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式