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1. 发明申请

WO2008011565A2 METHOD FOR IDENTIFYING NOVEL GENES 审中-公开
标题翻译：鉴定新基因的方法
公开(公告)号：WO2008011565A2
公开(公告)日：2008-01-24
申请号：PCT/US2007073983
申请日：2007-07-20
申请人： PIONEER HI BRED INT , DU PONT , ABAD ANDRE R , DONG HUA , LO SUSAN B , MCCUTCHEN BILLY F , SHI XIAOMEI
发明人： ABAD ANDRE R , DONG HUA , LO SUSAN B , MCCUTCHEN BILLY F , SHI XIAOMEI
IPC分类号： C12Q1/68
CPC分类号： C12N15/8286 , C12Q1/6813 , Y02A40/162 , C12Q2531/113
摘要： Methods and compositions for identifying novel genes that share regions of homology with known genes from target groups of genes of interest are provided. The methods comprise systematically designing oligonucleotide primers that are specific for regions of homology within the nucleotide sequences of a target group of known genes and performing successive rounds of PCR amplification of nucleic acid material from an organism of interest. The PCR steps are intended to identify and amplify nucleic acids comprising both known and novel genes. Nucleic acid molecules comprising known genes are detected and eliminated from further consideration by dot blot analysis using oligonucleotide probes specific for the known genes in the target group. Potentially novel genes are subjected to further sequence analysis to confirm novelty and assayed for biological activity. Compositions of the present invention include novel polynucleotides, and variants and fragments thereof, that comprise novel genes and the polypeptides encoded thereby.
摘要翻译：提供了用于鉴定与来自目标基因目标组的已知基因具有同源性区域的新基因的方法和组合物。所述方法包括系统地设计对已知基因的目标组的核苷酸序列内的同源性区域特异的寡核苷酸引物，并且从感兴趣的生物进行核酸材料的连续循环PCR扩增。 PCR步骤旨在鉴定和扩增包含已知和新型基因的核酸。包含已知基因的核酸分子通过使用对靶基因中已知基因具有特异性的寡核苷酸探针的斑点印迹分析进一步考虑而被检测和消除。对潜在的新基因进行进一步的序列分析以确认新颖性并测定生物活性。本发明的组合物包括新的多核苷酸及其变体和片段，其包含新基因和由此编码的多肽。

2. 发明申请

WO2006133182A3 INSECT-SPECIFIC PROTEASE RECOGNITION SEQUENCES 审中-公开
标题翻译： INSECT-SPECIFIC PROTEASE识别序列
公开(公告)号：WO2006133182A3
公开(公告)日：2007-02-08
申请号：PCT/US2006021902
申请日：2006-06-06
申请人： PIONEER HI BRED INT , DU PONT , ABAD ANDRE R , CHUN GARY C , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J
发明人： ABAD ANDRE R , CHUN GARY C , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J
IPC分类号： C07K14/325 , A01N63/02 , C12N9/64 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。本发明的具体实施方案还提供改性的昆虫原毒素和修饰的昆虫毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

3. 发明申请

WO2006028495A2 LIPASES AND METHODS OF USE 审中-公开
标题翻译：脂类和使用方法
公开(公告)号：WO2006028495A2
公开(公告)日：2006-03-16
申请号：PCT/US2005005178
申请日：2005-02-18
申请人： PIONEER HI BRED INT , DU PONT , ABAD ANDRE R , MCCUTCHEN BILLY F , WONG JAMES F , YU CAO GUO
发明人： ABAD ANDRE R , MCCUTCHEN BILLY F , WONG JAMES F , YU CAO GUO
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/8286 , C12N9/20 , Y02A40/162
摘要： Methods of creating and enhancing insect resistance in plants by introducing non-plant lipases into plants are provided. Plants with enhanced insect resistance and seed from plants thereof are provided. DNA sequences encoding insecticidal lipases and insecticidal lipase gene products that are useful in the practice of this invention are also provided. The compositions and methods of the invention may be used in a variety of agricultural systems for controlling pests, including propagating lineages of insect-resistant crops and targeting expression of these insecticidal lipases to plant organs that are particularly susceptible to infestation, such as roots and leaves.
摘要翻译：提供了通过将非植物脂肪酶引入植物中来在植物中产生和增强昆虫抗性的方法。提供了具有增强的昆虫抗性的植物和来自植物的种子。还提供了编码用于本发明实践的杀虫脂肪酶和杀虫脂肪酶基因产物的DNA序列。本发明的组合物和方法可以用于各种农业系统中以控制害虫，包括繁殖抗虫作物的谱系并将这些杀虫脂肪酶的靶向表达靶向特别容易侵染的植物器官，例如根和叶。

4. 发明申请

WO2006107954A3 METHODS AND COMPOSITIONS FOR DESIGNING NUCLEIC ACID MOLECULES FOR POLYPEPTIDE EXPRESSION IN PLANTS USING PLANT VIRUS CODON-BIAS 审中-公开
标题翻译：使用植物病毒CODON-BIAS设计植物中多肽表达的核酸分子的方法和组合物
公开(公告)号：WO2006107954A3
公开(公告)日：2007-02-22
申请号：PCT/US2006012478
申请日：2006-04-04
申请人： PIONEER HI BRED INT , DU PONT , SIMMONS CARL R , ABAD ANDRE R , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F
发明人： ABAD ANDRE R , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F
IPC分类号： C12N15/82 , A01H5/00 , C12N15/67
CPC分类号： C12N15/8216 , C12N15/8286 , Y02A40/162
摘要： The present invention relates to methods of designing nucleic acid molecules for improved expression of the encoded polypeptides in plants. In such methods, codon usage frequencies are biased towards codon usage frequencies of a plant virus, group of plant viruses, or a subset of nucleic acid molecules therefrom. In preferred embodiments, the encoded polypeptide affects the phenotype of the plant. The invention also pertains to nucleic acid molecules encoding insecticidal polypeptides wherein the nucleic acid molecules have been designed to have plant virus codon-biased. The invention also pertains to transgenic plants and progeny thereof with increased expression of insecticidal polypeptides for improved resistance to insects and other pests that are detrimental to plants of agricultural value.
摘要翻译：本发明涉及设计核酸分子以改进植物中编码多肽表达的方法。在这种方法中，密码子使用频率偏向于植物病毒，植物病毒组或其核酸分子的子集的密码子使用频率。在优选的实施方案中，编码的多肽影响植物的表型。本发明还涉及编码杀虫多肽的核酸分子，其中核酸分子被设计为具有植物病毒密码子偏倚。本发明还涉及具有增加的杀虫多肽表达的转基因植物及其后代，以改善对对农业价值的植物有害的昆虫和其它害虫的抗性。

5. 发明申请

WO2005083095A3 METHODS FOR ENHANCING INSECT RESISTANCE IN PLANTS 审中-公开
标题翻译：提高植物抗病性的方法
公开(公告)号：WO2005083095A3
公开(公告)日：2006-03-09
申请号：PCT/US2005005177
申请日：2005-02-18
申请人： PIONEER HI BRED INT , DU PONT , ABAD ANDRE R , MCCUTCHEN BILLY F
发明人： ABAD ANDRE R , MCCUTCHEN BILLY F
IPC分类号： A01H1/00 , A01H5/00 , C12N15/82
CPC分类号： C12N15/8286 , Y02A40/162
摘要： Methods for creating and enhancing insect resistance in plants are provided. The methods comprise stably introducing into a plant a combination of polynucleotides comprising a sequence encoding a lipase polypeptide having insecticidal activity and a sequence encoding a Bt insecticidal protein, where each of these coding sequences is operably linked to a promoter that drives expression in a plant cell. Plants with enhanced insect resistance and seed thereof are also provided. The methods of the invention may be used in a variety of agricultural systems for controlling insect pests, including propagating lineages of insect-resistant crops and targeting coexpression of insecticidal lipase and Btinsecticidal protein to plant organs that are particularly susceptible to infestation.
摘要翻译：提供了建立和增强植物抗虫性的方法。所述方法包括将包含编码具有杀虫活性的脂肪酶多肽的序列和编码Bt杀虫蛋白的序列的多核苷酸的组合稳定地引入到植物中，其中每个编码序列与驱动植物细胞中表达的启动子可操作地连接。还提供了具有增强的抗虫性和种子的植物。本发明的方法可用于控制害虫的各种农业系统中，包括传播昆虫抗性作物的谱系，并将杀虫脂肪酶和杀真菌蛋白的共表达靶向特别易受侵染的植物器官。

6. 发明申请

WO2005066349A3 GENES ENCODING PROTEINS WITH PESTICIDAL ACTIVITY 审中-公开
标题翻译：用杀虫活性编码蛋白质的基因
公开(公告)号：WO2005066349A3
公开(公告)日：2005-10-27
申请号：PCT/US2004041530
申请日：2004-12-09
申请人： PIONEER HI BRED INT , DU PONT , ABAD ANDRE , DONG HUA , HERRMANN RAFAEL , LU ALBERT , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J , WONG JAMES F
发明人： ABAD ANDRE , DONG HUA , HERRMANN RAFAEL , LU ALBERT , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J , WONG JAMES F
IPC分类号： C07K14/325 , C12N15/82
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide pesticidal polypeptide compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. These compositions find use in methods for controlling pests, especially plant pests. Novel plant proteases, sequences encoding these proteases, and methods for their use are also provided.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。本发明提供已被工程化以编码具有增加的抗植物蛋白酶对蛋白水解降解的杀虫多肽的诱变的核酸。特别地，提供了编码杀虫多肽的核酸序列，其被修饰为包含赋予植物蛋白酶降解或蛋白水解灭活抗性的蛋白水解保护位点。本发明的具体实施方案提供杀虫多肽组合物和制剂，表达盒和转化植物，植物细胞和种子。这些组合物可用于防治害虫，特别是植物害虫的方法。还提供了新型植物蛋白酶，编码这些蛋白酶的序列及其使用方法。

7. 发明申请

WO2006083513A3 AN INDUCIBLE DEOXYHYPUSINE SYNTHASE PROMOTER FROM MAIZE 审中-公开
标题翻译：一种可诱导的脱氧吉他合成酶促进剂
公开(公告)号：WO2006083513A3
公开(公告)日：2007-03-01
申请号：PCT/US2006001001
申请日：2006-01-11
申请人： PIONEER HI BRED INT , DU PONT , DIEHN SCOTT , LU ALBERT L , MCCUTCHEN BILLY F , SIMS LYNNE E , WARD KIM R
发明人： DIEHN SCOTT , LU ALBERT L , MCCUTCHEN BILLY F , SIMS LYNNE E , WARD KIM R
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N9/0004 , C12N15/8227 , C12N15/8237 , C12N15/8239
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding a maize deoxyhypusine synthase. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码玉米脱氧酪氨酸合成酶的基因的诱导型启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

8. 发明申请

WO2006066054A3 A ROOT-PREFERRED, WOUND- AND INSECT-INDUCIBLE 2-OXOGLUTARATE-DEPENDENT OXYGENASE PROMOTER FROM MAIZE AND ITS USE 审中-公开
标题翻译：来自玉米的一种根源优选的，伤口和昆虫诱导的2-氧代乳酸依赖性的氧化酶启动子及其用途
公开(公告)号：WO2006066054A3
公开(公告)日：2006-08-24
申请号：PCT/US2005045527
申请日：2005-12-15
申请人： PIONEER HI BRED INT , DU PONT , DIEHN SCOTT , LU ALBERT L , MCCUTCHEN BILLY F , SIMS LYNNE E , WARD KIM R
发明人： DIEHN SCOTT , LU ALBERT L , MCCUTCHEN BILLY F , SIMS LYNNE E , WARD KIM R
IPC分类号： C12N15/82 , A01H5/00 , C12N15/29
CPC分类号： C12N9/0069 , C07K14/415 , C12N15/8227 , C12N15/8237 , C12N15/8239
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding a maize 2-oxoglutarate-dependent oxygenase. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码玉米2-氧代戊二酸依赖性加氧酶的基因的诱导型启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子有效连接的核苷酸序列稳定地并入植物细胞的基因组，并再生表达该核苷酸序列的稳定转化的植物。

9. 发明申请

WO02086072A8 ANTIMICROBIAL POLYPEPTIDES AND THEIR USES 审中-公开
标题翻译：抗微生物多肽及其用途
公开(公告)号：WO02086072A8
公开(公告)日：2002-12-12
申请号：PCT/US0212511
申请日：2002-04-19
申请人： PIONEER HI BRED INT , ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H , DU PONT
发明人： ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H
IPC分类号： C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N7/01 , C12N15/12 , C12N15/82 , C12N
CPC分类号： C12N15/8282 , C07K14/43563 , Y10S530/858
摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, an seed thereof, transformed with the nucleic acid mole cules of the invention so as to confer disease resistance on the plant.
摘要翻译：本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。进一步提供了用本发明的核酸摩尔转化的植物细胞，植物及其种子，从而赋予植物抗病性。

10. 发明申请

WO02086072A3 ANTIMICROBIAL POLYPEPTIDES AND THEIR USES 审中-公开
标题翻译：抗微生物多肽及其用途
公开(公告)号：WO02086072A3
公开(公告)日：2003-06-05
申请号：PCT/US0212511
申请日：2002-04-19
申请人： PIONEER HI BRED INT , ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H , DU PONT
发明人： ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H
IPC分类号： C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N7/01 , C12N15/12 , C12N15/82 , A01H5/00 , C12N5/04 , C12N15/70 , C12N15/74 , C12N15/80 , C12N15/81
CPC分类号： C12N15/8282 , C07K14/43563 , Y10S530/858
摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, an seed thereof, transformed with the nucleic acid mole cules of the invention so as to confer disease resistance on the plant.
摘要翻译：本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。进一步提供了用本发明的核酸摩尔转化的植物细胞，植物及其种子，从而赋予植物抗病性。

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