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1. 发明申请

WO2006107954A3 METHODS AND COMPOSITIONS FOR DESIGNING NUCLEIC ACID MOLECULES FOR POLYPEPTIDE EXPRESSION IN PLANTS USING PLANT VIRUS CODON-BIAS 审中-公开
标题翻译：使用植物病毒CODON-BIAS设计植物中多肽表达的核酸分子的方法和组合物
公开(公告)号：WO2006107954A3
公开(公告)日：2007-02-22
申请号：PCT/US2006012478
申请日：2006-04-04
申请人： PIONEER HI BRED INT , DU PONT , SIMMONS CARL R , ABAD ANDRE R , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F
发明人： ABAD ANDRE R , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F
IPC分类号： C12N15/82 , A01H5/00 , C12N15/67
CPC分类号： C12N15/8216 , C12N15/8286 , Y02A40/162
摘要： The present invention relates to methods of designing nucleic acid molecules for improved expression of the encoded polypeptides in plants. In such methods, codon usage frequencies are biased towards codon usage frequencies of a plant virus, group of plant viruses, or a subset of nucleic acid molecules therefrom. In preferred embodiments, the encoded polypeptide affects the phenotype of the plant. The invention also pertains to nucleic acid molecules encoding insecticidal polypeptides wherein the nucleic acid molecules have been designed to have plant virus codon-biased. The invention also pertains to transgenic plants and progeny thereof with increased expression of insecticidal polypeptides for improved resistance to insects and other pests that are detrimental to plants of agricultural value.
摘要翻译：本发明涉及设计核酸分子以改进植物中编码多肽表达的方法。在这种方法中，密码子使用频率偏向于植物病毒，植物病毒组或其核酸分子的子集的密码子使用频率。在优选的实施方案中，编码的多肽影响植物的表型。本发明还涉及编码杀虫多肽的核酸分子，其中核酸分子被设计为具有植物病毒密码子偏倚。本发明还涉及具有增加的杀虫多肽表达的转基因植物及其后代，以改善对对农业价值的植物有害的昆虫和其它害虫的抗性。

2. 发明申请

WO2006133182A3 INSECT-SPECIFIC PROTEASE RECOGNITION SEQUENCES 审中-公开
标题翻译： INSECT-SPECIFIC PROTEASE识别序列
公开(公告)号：WO2006133182A3
公开(公告)日：2007-02-08
申请号：PCT/US2006021902
申请日：2006-06-06
申请人： PIONEER HI BRED INT , DU PONT , ABAD ANDRE R , CHUN GARY C , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J
发明人： ABAD ANDRE R , CHUN GARY C , FLANNAGAN RONALD D , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , RICE JANET A , SCHEPERS ERIC J
IPC分类号： C07K14/325 , A01N63/02 , C12N9/64 , C12N15/82
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。本发明的具体实施方案还提供改性的昆虫原毒素和修饰的昆虫毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

3. 发明申请

WO02086072A8 ANTIMICROBIAL POLYPEPTIDES AND THEIR USES 审中-公开
标题翻译：抗微生物多肽及其用途
公开(公告)号：WO02086072A8
公开(公告)日：2002-12-12
申请号：PCT/US0212511
申请日：2002-04-19
申请人： PIONEER HI BRED INT , ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H , DU PONT
发明人： ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H
IPC分类号： C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N7/01 , C12N15/12 , C12N15/82 , C12N
CPC分类号： C12N15/8282 , C07K14/43563 , Y10S530/858
摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, an seed thereof, transformed with the nucleic acid mole cules of the invention so as to confer disease resistance on the plant.
摘要翻译：本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。进一步提供了用本发明的核酸摩尔转化的植物细胞，植物及其种子，从而赋予植物抗病性。

4. 发明申请

WO02086072A3 ANTIMICROBIAL POLYPEPTIDES AND THEIR USES 审中-公开
标题翻译：抗微生物多肽及其用途
公开(公告)号：WO02086072A3
公开(公告)日：2003-06-05
申请号：PCT/US0212511
申请日：2002-04-19
申请人： PIONEER HI BRED INT , ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H , DU PONT
发明人： ALTIER DANIEL J , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , WEAVER JANINE L , WONG JAMES F H
IPC分类号： C07K14/435 , C12N1/15 , C12N1/19 , C12N1/21 , C12N7/01 , C12N15/12 , C12N15/82 , A01H5/00 , C12N5/04 , C12N15/70 , C12N15/74 , C12N15/80 , C12N15/81
CPC分类号： C12N15/8282 , C07K14/43563 , Y10S530/858
摘要： The methods and compositions of the present invention find use in impacting microbial pathogens and in enhancing disease resistance to pathogens, particularly by plants. The compositions of the invention include polypeptides that possess antimicrobial properties, particularly fungicidal properties, and the encoding nucleic acid molecules. The polypeptides of the invention are isolated from the hemolymph and fat bodies of insect larvae induced by injection of plant pathogenic fungi. Further provided are plant cells, plants, an seed thereof, transformed with the nucleic acid mole cules of the invention so as to confer disease resistance on the plant.
摘要翻译：本发明的方法和组合物用于影响微生物病原体并增强对病原体的抗病性，特别是植物的抗病性。本发明的组合物包括具有抗微生物特性，特别是杀真菌性质和编码核酸分子的多肽。通过注射植物致病真菌诱导的昆虫幼虫的血淋巴和脂肪体分离本发明的多肽。进一步提供了用本发明的核酸摩尔转化的植物细胞，植物及其种子，从而赋予植物抗病性。

5. 发明申请

WO2006083513A3 AN INDUCIBLE DEOXYHYPUSINE SYNTHASE PROMOTER FROM MAIZE 审中-公开
标题翻译：一种可诱导的脱氧吉他合成酶促进剂
公开(公告)号：WO2006083513A3
公开(公告)日：2007-03-01
申请号：PCT/US2006001001
申请日：2006-01-11
申请人： PIONEER HI BRED INT , DU PONT , DIEHN SCOTT , LU ALBERT L , MCCUTCHEN BILLY F , SIMS LYNNE E , WARD KIM R
发明人： DIEHN SCOTT , LU ALBERT L , MCCUTCHEN BILLY F , SIMS LYNNE E , WARD KIM R
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N9/0004 , C12N15/8227 , C12N15/8237 , C12N15/8239
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding a maize deoxyhypusine synthase. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码玉米脱氧酪氨酸合成酶的基因的诱导型启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将可操作地连接到本发明的根优选启动子的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

6. 发明申请

WO2006066054A3 A ROOT-PREFERRED, WOUND- AND INSECT-INDUCIBLE 2-OXOGLUTARATE-DEPENDENT OXYGENASE PROMOTER FROM MAIZE AND ITS USE 审中-公开
标题翻译：来自玉米的一种根源优选的，伤口和昆虫诱导的2-氧代乳酸依赖性的氧化酶启动子及其用途
公开(公告)号：WO2006066054A3
公开(公告)日：2006-08-24
申请号：PCT/US2005045527
申请日：2005-12-15
申请人： PIONEER HI BRED INT , DU PONT , DIEHN SCOTT , LU ALBERT L , MCCUTCHEN BILLY F , SIMS LYNNE E , WARD KIM R
发明人： DIEHN SCOTT , LU ALBERT L , MCCUTCHEN BILLY F , SIMS LYNNE E , WARD KIM R
IPC分类号： C12N15/82 , A01H5/00 , C12N15/29
CPC分类号： C12N9/0069 , C07K14/415 , C12N15/8227 , C12N15/8237 , C12N15/8239
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for an inducible promoter for the gene encoding a maize 2-oxoglutarate-dependent oxygenase. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the root-preferred promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码玉米2-氧代戊二酸依赖性加氧酶的基因的诱导型启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的根优选启动子有效连接的核苷酸序列稳定地并入植物细胞的基因组，并再生表达该核苷酸序列的稳定转化的植物。

7. 发明申请

WO2009091862A2 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LEPIDOPTERA 审中-公开
标题翻译：用于抑制来自LEPIDOPTERA的目标多核苷酸的组合物和方法
公开(公告)号：WO2009091862A2
公开(公告)日：2009-07-23
申请号：PCT/US2009031055
申请日：2009-01-15
申请人： PIONEER HI BRED INT , DU PONT , HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
发明人： HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N65/20 , A01N65/44 , C12N15/113 , C12N2310/11 , C12N2310/14 , C12N2310/531 , C12N2320/32 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides encoding polypeptides from specific protein families and various target polynucleotides set forth in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当沉积元件在被害虫如来自鳞翅目序列的害虫中摄取时，它们能够降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供编码来自特定蛋白质家族的多肽的多核苷酸和SEQ ID NO：1,2,3,4,5,6,7,8,9,10,11,12,13,14中所示的各种靶多核苷酸的靶多核苷酸，15，16，17，18，19，20，21，22，23，24，25，26，27，28，29，30，31，32，33，34，35，36，37，38，39 ，40,41,42,43,44,45,46,47,48,49或50个或其活性变体和片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即，具有杀虫活性）。进一步提供的是沉积元件，当被害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是草地夜蛾（Spodoptera frugiperda）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

8. 发明申请

WO2009091864A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LYGUS 审中-公开
标题翻译：抑制淋巴瘤靶向多核苷酸的组合物和方法
公开(公告)号：WO2009091864A3
公开(公告)日：2009-12-10
申请号：PCT/US2009031057
申请日：2009-01-15
申请人： PIONEER HI BRED INT , DU PONT , HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
发明人： HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , A01N57/16 , C12N15/113 , C12N15/8218 , C12N2310/141 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, and further provides target polynucleotides set forth in SEQ ID NOS:1-21 or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Lygus Hesperus. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉默元件的方法和组合物，所述沉默元件当被有害生物如来自草盲蝽属的害虫摄入时，能够降低害虫中靶序列的表达。在具体的实施方案中，靶序列表达的降低控制害虫，并且由此该方法和组合物能够限制对植物的损害。本发明提供了来自本文公开的特定多肽家族的各种靶多核苷酸，并且还提供了SEQ ID NO：1-21中列出的靶多核苷酸或其活性变体，其中靶害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性）。进一步提供了沉默元件，其在被害虫摄入时降低靶多肽的水平并由此控制害虫。在具体的实施方案中，害虫是草盲蝽（Lygus Hesperus）。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其他宿主细胞。

9. 发明申请

WO2009091860A3 COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM THE FAMILY APHIDIDAE 审中-公开
标题翻译：用于抑制来自家庭蚜虫目标多核苷酸的组合物和方法
公开(公告)号：WO2009091860A3
公开(公告)日：2009-11-05
申请号：PCT/US2009031053
申请日：2009-01-15
申请人： PIONEER HI BRED INT , DU PONT , HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
发明人： HERRMANN RAFAEL , LASSNER MICHAEL , LU ALBERT L , NELSON MARK , PRESNAIL JAMES K , RICE JANET A
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , C07K14/43563 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Aphididae family, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides target polynucleotides for specific protein classes and also target polynucleotides as set forth in SEQ ID NOS:1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or active variants or fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Aphis glycines. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉默元件的方法和组合物，所述沉默元件当被有害生物例如来自蚜科的害虫摄取时，能够降低害虫中靶序列的表达。在具体的实施方案中，靶序列表达的降低控制害虫，并且由此该方法和组合物能够限制对植物的损害。本发明提供了特定蛋白质类别的靶多核苷酸以及如SEQ ID NO：1,2,3,4,5,6,7,8,9,10或其活性变体或片段所示的靶多核苷酸，其中a 目标害虫中一个或多个序列表达的减少控制了害虫（即，具有杀虫活性）。进一步提供了沉默元件，其在被害虫摄入时降低靶多肽的水平并由此控制害虫。在具体的实施方案中，害虫是蚜虫（Aphis）甘氨酸。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其他宿主细胞。

10. 发明申请

WO2006041917A3 AN INSECTICIDAL PLANT CYCLOTIDE WITH ACTIVITY AGAINST HOMOPTERAN INSECTS 审中-公开
标题翻译：具有抗肿瘤活性的杀虫剂植物循环
公开(公告)号：WO2006041917A3
公开(公告)日：2006-06-22
申请号：PCT/US2005035821
申请日：2005-10-04
申请人： DU PONT , HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , RICE JANET A , WAINE CLEMENT W K
发明人： HERRMANN RAFAEL , LU ALBERT L , MCCUTCHEN BILLY F , PRESNAIL JAMES K , RICE JANET A , WAINE CLEMENT W K
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C07K14/415 , C12N15/8286 , Y02A40/162
摘要： The present invention relates to isolated nucleic acids encoding plant cyclotides. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.
摘要翻译：本发明涉及编码植物环肽的分离的核酸。本发明还涉及在正义或反义方向上编码全部或部分植物环肽的嵌合基因的构建，其中嵌合基因的表达导致在转化的宿主细胞中产生改变水平的植物环磷酰胺。

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