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    • 3. 发明授权
    • Enzymes and microorganisms having amidase activity for hydrolysing polyamides
    • 具有用于水解聚酰胺的酰胺酶活性的酶和微生物
    • US06214592B1
    • 2001-04-10
    • US09000064
    • 1998-04-03
    • Joël CrouzetOlivier Favre-BulleCatherine JourdatAnne-Marie Le CoqDominique Petre
    • Joël CrouzetOlivier Favre-BulleCatherine JourdatAnne-Marie Le CoqDominique Petre
    • C12P1302
    • C12R1/15C12N9/80
    • The present invention relates to an enzyme with amidase activity, particularly towards substrates of the oligomer type derived from PA 6.6 (formula I) and/or PA 6 (formula II), said enzyme being characterized by a ratio Ra =   ⁢ enzymatic ⁢   ⁢ activity ⁢   ⁢ towards ⁢   ⁢ (poly)amide ⁢   ⁢ substrates ⁢   ⁢ of ⁢   ⁢ formula ⁢   ⁢   ⁢ ( I ) ⁢   ⁢     ⁢ enzymatic ⁢   ⁢ activity ⁢   ⁢ towards ⁢   ⁢ (poly)amide ⁢   ⁢ substrates ⁢ of ⁢   ⁢   ⁢ formul ⁢   ⁢ a ⁢   ⁢ ( ⁢ II) ⁢   = E .   ⁢ A .   ⁢ ( I ) E .   ⁢ A .   ⁢ ( II )   which is greater than 2, preferably greater than or equal to 10 and particularly preferably greater than or equal to 50. More precisely, this enzyme consists of the peptide sequence corresponding to the attached sequence SEQ ID NO: 2. The invention further relates to the DNA which codes for this polypeptide SEQ ID NO: 2 and whose sequence corresponds to the attached sequence SEQ ID NO: 1. The invention further relates to the microorganisms capable of producing this enzyme and to the hydrolysis process in which this enzyme and/or these microorganisms are applied.
    • 本发明涉及具有酰胺酶活性的酶,特别是涉及衍生自PA 6.6(式I)和/或PA 6(式II)的低聚物类型的底物,所述酶的特征在于大于2,优选更大 更优选地,该酶由对应于所附连接序列SEQ ID NO:2的肽序列组成。本发明还涉及编码该多肽SEQ ID NO: NO:2,其序列对应于所附序列SEQ ID NO:1。本发明还涉及能够产生该酶的微生物和其中施用该酶和/或这些微生物的水解过程。