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1. 发明授权

US06214592B1 Enzymes and microorganisms having amidase activity for hydrolysing polyamides 失效
标题翻译：具有用于水解聚酰胺的酰胺酶活性的酶和微生物
公开(公告)号：US06214592B1
公开(公告)日：2001-04-10
申请号：US09000064
申请日：1998-04-03
申请人： Joël Crouzet , Olivier Favre-Bulle , Catherine Jourdat , Anne-Marie Le Coq , Dominique Petre
发明人： Joël Crouzet , Olivier Favre-Bulle , Catherine Jourdat , Anne-Marie Le Coq , Dominique Petre
IPC分类号： C12P1302
CPC分类号： C12R1/15 , C12N9/80
摘要： The present invention relates to an enzyme with amidase activity, particularly towards substrates of the oligomer type derived from PA 6.6 (formula I) and/or PA 6 (formula II), said enzyme being characterized by a ratio Ra = ⁢ enzymatic ⁢ ⁢ activity ⁢ ⁢ towards ⁢ ⁢ (poly)amide ⁢ ⁢ substrates ⁢ ⁢ of ⁢ ⁢ formula ⁢ ⁢ ⁢ ( I ) ⁢ ⁢ ⁢ enzymatic ⁢ ⁢ activity ⁢ ⁢ towards ⁢ ⁢ (poly)amide ⁢ ⁢ substrates ⁢ of ⁢ ⁢ ⁢ formul ⁢ ⁢ a ⁢ ⁢ ( ⁢ II) ⁢ = E . ⁢ A . ⁢ ( I ) E . ⁢ A . ⁢ ( II ) which is greater than 2, preferably greater than or equal to 10 and particularly preferably greater than or equal to 50. More precisely, this enzyme consists of the peptide sequence corresponding to the attached sequence SEQ ID NO: 2. The invention further relates to the DNA which codes for this polypeptide SEQ ID NO: 2 and whose sequence corresponds to the attached sequence SEQ ID NO: 1. The invention further relates to the microorganisms capable of producing this enzyme and to the hydrolysis process in which this enzyme and/or these microorganisms are applied.
摘要翻译：本发明涉及具有酰胺酶活性的酶，特别是涉及衍生自PA 6.6（式I）和/或PA 6（式II）的低聚物类型的底物，所述酶的特征在于大于2，优选更大更优选地，该酶由对应于所附连接序列SEQ ID NO：2的肽序列组成。本发明还涉及编码该多肽SEQ ID NO： NO：2，其序列对应于所附序列SEQ ID NO：1。本发明还涉及能够产生该酶的微生物和其中施用该酶和/或这些微生物的水解过程。

2. 发明授权

US06180388B2 Enzymes and micro organisms with amidase activity which hydrolyze polyamides 失效
标题翻译：具有水解聚酰胺的酰胺酶活性的酶和微生物
公开(公告)号：US06180388B2
公开(公告)日：2001-01-30
申请号：US09000040
申请日：1998-04-03
申请人： Jo{umlaut over (e)}l Crouzet , Didier Faucher , Olivier Favre-Bulle , Catherine Jourdat , Dominique Petre , J{acute over (e)}r{circumflex over (o)}me Pierrard , Denis Thibaut , Carole Guitton
发明人： Jo{umlaut over (e)}l Crouzet , Didier Faucher , Olivier Favre-Bulle , Catherine Jourdat , Dominique Petre , J{acute over (e)}r{circumflex over (o)}me Pierrard , Denis Thibaut , Carole Guitton
IPC分类号： C12N980
CPC分类号： C12R1/01 , C12N9/80
摘要： The present invention relates to a process for the enzymatic hydrolysis of polyamides 6.6 to give adipic acid monomers and hexamethylenediamine monomers. The present invention further relates to an enzyme with amidase activity particularly towards substrates of the oligomer type derived from PA 6.6 and/or PA 6, said enzyme being characterized in that it consists of a peptide sequence corresponding to SEQ ID NO: 1 in the attached sequence listing and/or at least one polypeptide homologous to this sequence. The invention further relates to the DNA coding for said enzyme and to the biological precursors thereof The invention further relates to the microorganisms capable of producing this enzyme and to the hydrolysis process in which this enzyme and/or these microorganisms are applied.
摘要翻译：本发明涉及聚酰胺6.6的酶水解以得到己二酸单体和六亚甲基二胺单体的方法。本发明还涉及具有酰胺酶活性的酶，特别是衍生自PA 6.6和/或PA 6的低聚物类型的底物，所述酶的特征在于其由相应于所附载体中SEQ ID NO：1的肽序列组成序列表和/或与该序列同源的至少一种多肽。本发明还涉及编码所述酶及其生物前体的DNA。本发明还涉及能够产生该酶的微生物和其中施用该酶和/或这些微生物的水解过程。

3. 发明授权

US07361507B1 Method for modulating nitrilase selectivity, nitrilases obtained by said method and use thereof 失效
标题翻译：调节腈水解酶选择性的方法，通过所述方法获得的腈水解酶及其用途
公开(公告)号：US07361507B1
公开(公告)日：2008-04-22
申请号：US10129560
申请日：2000-10-30
申请人： Jérôme Pierrard , Olivier Favre-Bulle , Catherine Jourdat
发明人： Jérôme Pierrard , Olivier Favre-Bulle , Catherine Jourdat
IPC分类号： C12N15/00 , C12Q1/34 , C12N9/78 , C12P21/06 , G01N33/00 , C07K14/00 , C07H21/04
CPC分类号： C12N9/78 , Y10T436/143333
摘要： The invention concerns a modified nitrilase with modulated selectivity, characterized in that it comprises in position 162 an amino acid residue different from the original amino acid residue.
摘要翻译：本发明涉及一种具有调节选择性的修饰的腈水解酶，其特征在于其在位置162处包含不同于原始氨基酸残基的氨基酸残基。

4. 发明授权

US06537583B1 Method for preparing capsules consisting of a liquid active material core enclosed in a mineral coating 失效
标题翻译：制备胶囊的方法，由封闭在矿物涂层中的液体活性物质核心组成
公开(公告)号：US06537583B1
公开(公告)日：2003-03-25
申请号：US09830797
申请日：2001-07-19
申请人： Dominique Dupuis , Catherine Jourdat
发明人： Dominique Dupuis , Catherine Jourdat
IPC分类号： A61K916
CPC分类号： B01J13/06 , Y10T428/2984
摘要： The invention concerns a method for preparing mineral capsules consisting of aqueous liquid core enclosed in mineral coating, said method consisting in: 1) emulsifying an aqueous fluid in a phase non-miscible with said aqueous fluid so as to disperse it therein in the form of droplets; 2) contacting, in the resulting emulsion, at least a zirconium, silicon, aluminium and/or a transition metal capable of being hydrolysed or subjected to condensation polymerisation in temperature and pH conditions suitable for forming a precipitate consisting of the corresponding oxide or hydroxide; 3) recuperating the resulting mineral capsules and, if required, purifying them. Said method is characterised in that the formation of the mineral precipitate in the second step is carried out in the presence of an amphiphilic surfactant system present in the emulsion and capable of concentrating the deposit of mineral particles of said precipitate formed in the interface of the aqueous droplets and the hydrophobic phase and of effectively blocking their diffusion inside the droplets.
摘要翻译：本发明涉及一种制备矿物胶囊的方法，所述矿物胶囊由包封在矿物涂层中的水性液体核心组成，所述方法包括：1）将水性流体乳化在与所述含水流体不可混溶的相中，以便将其分散在其中液滴 2）在所得乳液中，至少一种能够在适于形成由相应的氧化物或氢氧化物组成的沉淀物的温度和pH条件下水解或进行缩聚的锆，硅，铝和/或过渡金属; 3）恢复所得的矿物胶囊，如果需要，将其净化。所述方法的特征在于，在第二步中形成矿物沉淀物是在存在于乳液中的两亲表面活性剂体系的存在下进行的，并且能够浓缩形成在水性界面中的所述沉淀物的矿物颗粒沉积物液滴和疏水相，并有效地阻止其在液滴内的扩散。

5. 发明申请

US20050260725A1 Process for the preparation of trans-carveol 审中-公开
标题翻译：制备反式香芹醇的方法
公开(公告)号：US20050260725A1
公开(公告)日：2005-11-24
申请号：US10416125
申请日：2001-11-08
申请人： Wouter Duetz , Bernard Witholt , Catherine Jourdat
发明人： Wouter Duetz , Bernard Witholt , Catherine Jourdat
IPC分类号： C12N15/09 , C12P7/02 , C12P7/24 , C12P7/26 , C12R1/01 , C12P7/22
CPC分类号： C12P7/02 , C12P7/26
摘要： A process for the production of trans-carveol which process comprises (a) incubading in the presence of D-limonene, a culture medium comprising microbial cells expressing an oxygenase capable of toluene and/or napththalene and (b) recovering the trans-carveol from the culture medium.
摘要翻译：一种生产反式香芹醇的方法，该方法包括（a）在D-柠檬烯的存在下孵育，包含表达能够甲苯和/或萘的加氧酶的微生物细胞的培养基和（b）从培养基。

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