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1. 发明授权

US06214592B1 Enzymes and microorganisms having amidase activity for hydrolysing polyamides 失效
标题翻译：具有用于水解聚酰胺的酰胺酶活性的酶和微生物
公开(公告)号：US06214592B1
公开(公告)日：2001-04-10
申请号：US09000064
申请日：1998-04-03
申请人： Joël Crouzet , Olivier Favre-Bulle , Catherine Jourdat , Anne-Marie Le Coq , Dominique Petre
发明人： Joël Crouzet , Olivier Favre-Bulle , Catherine Jourdat , Anne-Marie Le Coq , Dominique Petre
IPC分类号： C12P1302
CPC分类号： C12R1/15 , C12N9/80
摘要： The present invention relates to an enzyme with amidase activity, particularly towards substrates of the oligomer type derived from PA 6.6 (formula I) and/or PA 6 (formula II), said enzyme being characterized by a ratio Ra = ⁢ enzymatic ⁢ ⁢ activity ⁢ ⁢ towards ⁢ ⁢ (poly)amide ⁢ ⁢ substrates ⁢ ⁢ of ⁢ ⁢ formula ⁢ ⁢ ⁢ ( I ) ⁢ ⁢ ⁢ enzymatic ⁢ ⁢ activity ⁢ ⁢ towards ⁢ ⁢ (poly)amide ⁢ ⁢ substrates ⁢ of ⁢ ⁢ ⁢ formul ⁢ ⁢ a ⁢ ⁢ ( ⁢ II) ⁢ = E . ⁢ A . ⁢ ( I ) E . ⁢ A . ⁢ ( II ) which is greater than 2, preferably greater than or equal to 10 and particularly preferably greater than or equal to 50. More precisely, this enzyme consists of the peptide sequence corresponding to the attached sequence SEQ ID NO: 2. The invention further relates to the DNA which codes for this polypeptide SEQ ID NO: 2 and whose sequence corresponds to the attached sequence SEQ ID NO: 1. The invention further relates to the microorganisms capable of producing this enzyme and to the hydrolysis process in which this enzyme and/or these microorganisms are applied.
摘要翻译：本发明涉及具有酰胺酶活性的酶，特别是涉及衍生自PA 6.6（式I）和/或PA 6（式II）的低聚物类型的底物，所述酶的特征在于大于2，优选更大更优选地，该酶由对应于所附连接序列SEQ ID NO：2的肽序列组成。本发明还涉及编码该多肽SEQ ID NO： NO：2，其序列对应于所附序列SEQ ID NO：1。本发明还涉及能够产生该酶的微生物和其中施用该酶和/或这些微生物的水解过程。

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