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1. 发明授权

US4414325A Method for measurement of trace enzyme 失效
标题翻译：痕量酶的测定方法
公开(公告)号：US4414325A
公开(公告)日：1983-11-08
申请号：US298814
申请日：1981-09-02
申请人： Nobuhito Masuda , Yuji Mihara , Masaki Okazaki , Hajime Makiuchi
发明人： Nobuhito Masuda , Yuji Mihara , Masaki Okazaki , Hajime Makiuchi
IPC分类号： G01N33/536 , C12Q1/00 , C12Q1/34 , G01N33/53 , G01N33/52 , G01N33/54 , G01N33/58
CPC分类号： C12Q1/34 , C12Q1/00 , C12Q2334/00 , C12Q2337/00 , G01N2333/976 , Y10S435/962 , Y10S436/805
摘要： The amount of enzyme is determined by reacting an enzyme to be measured with a synthetic substrate having at least one structure to be specifically contacted with the enzyme and at least one spectral sensitizer structure, bringing either the reaction product having the spectral sensitizer structure or the unreacted synthetic substrate into contact with a silver halide emulsion layer, exposing the same to light of a spectral sensitizing wavelength region corresponding to the spectral sensitizer structure, developing the silver halide, and then measuring the optical density of developed silver and/or colored dye.
摘要翻译：酶的量通过使待测量的酶与具有至少一种与酶特异性接触的结构的合成底物和至少一种光谱增感剂结构反应，使具有光谱增感剂结构的反应产物或未反应的合成基底与卤化银乳剂层接触，将其暴露于对应于光谱敏化剂结构的光谱增感波长区域的光，显影卤化银，然后测量显影的银和/或着色染料的光密度。

2. 发明授权

US4472498A Analysis film and a method of analysis using the same 失效
标题翻译：分析胶片和使用其的分析方法
公开(公告)号：US4472498A
公开(公告)日：1984-09-18
申请号：US401771
申请日：1982-07-26
申请人： Nobuhito Masuda , Yukio Yasuda , Shigeru Nagatomo , Hajime Makiuchi , Masaki Okazaki
发明人： Nobuhito Masuda , Yukio Yasuda , Shigeru Nagatomo , Hajime Makiuchi , Masaki Okazaki
IPC分类号： G01N33/52 , G01N33/535 , G01N33/542 , G01N33/543 , G01N33/54 , G01N33/78
CPC分类号： G01N33/542 , G01N33/521 , G01N33/535 , G01N33/54386 , Y10S435/805 , Y10S435/97 , Y10S436/81 , Y10S436/815
摘要： An analysis film comprises a reagent layer composed of a porous material which contains an antibody but does not substantially contain a complex of an analyte or a labelled antigen with the antibody. In the analysis film, reagents for enzyme immune reaction of homogenous type are incorporated so that an analyte is analyzed without requiring B/F separation. An analysis method for various analytes using the same provides high sensitivity, high accuracy as well as good reproducibility and is simple and rapid.
摘要翻译：分析膜包括由含有抗体但基本上不含有分析物或标记抗原与抗体的复合物的多孔材料构成的试剂层。在分析膜中，并入用于均匀型酶免疫反应的试剂，从而分析分析物而不需要B / F分离。使用该方法的各种分析物的分析方法提供高灵敏度，高精度以及良好的再现性，并且简单快速。

3. 发明授权

US4587102A Multi-layer analysis element utilizing specific binding reaction 失效
标题翻译：利用特异性结合反应的多层分析元件
公开(公告)号：US4587102A
公开(公告)日：1986-05-06
申请号：US637324
申请日：1984-08-02
申请人： Shigeru Nagatomo , Yukio Yasuda , Nobuhito Masuda , Hajime Makiuchi , Masaki Okazaki
发明人： Shigeru Nagatomo , Yukio Yasuda , Nobuhito Masuda , Hajime Makiuchi , Masaki Okazaki
IPC分类号： G01N33/58 , G01N33/52 , G01N33/543 , G01N21/78 , G01N33/53
CPC分类号： G01N33/54386 , G01N33/525 , Y10S435/805 , Y10S436/81
摘要： A dry type multilayer analysis element for assaying a concentration of a specific component utilizing a competitive immunological reaction comprises a detection element comprising a detection layer which receives a labelled complex formed as a result of the competitive immunological reaction or an optically detectable change formed dependent upon an amount of the labelled complex of the specific component and having further provided thereon the detection layer a reaction layer comprising a fibrous porous medium containing fine particles therein. The multilayer analysis element absorbs an amount of a sample solution necessary for the competitive immunological reaction so that the multilayer analysis element has high sensitivity and high reproducibility.
摘要翻译：用于使用竞争性免疫反应测定特定成分的浓度的干式多层分析元件包括检测元件，其包含检测层，该检测层接受由于竞争性免疫反应而形成的标记复合物，或者由量的特定成分的标记复合物，并且还在其上提供检测层，反应层包含其中含有微粒的纤维状多孔介质。多层分析元件吸收竞争性免疫反应所需的样品溶液的量，使得多层分析元件具有高灵敏度和高再现性。

4. 发明授权

US4429050A Competitive immunochemical measurement of plural trace components involving spectral sensitizing dye labels 失效
标题翻译：涉及光谱增感染料标签的多种微量成分的竞争性免疫化学测定
公开(公告)号：US4429050A
公开(公告)日：1984-01-31
申请号：US298811
申请日：1981-09-02
申请人： Yukio Yasuda , Nobuhito Masuda , Yuji Mihara , Masaki Okazaki , Nobuo Hiratsuka
发明人： Yukio Yasuda , Nobuhito Masuda , Yuji Mihara , Masaki Okazaki , Nobuo Hiratsuka
IPC分类号： G03C5/00 , G01N33/532 , G01N33/536 , G01N33/58 , G03C1/12 , G03C5/04 , G01N33/54 , G01N33/52 , G01N33/74
CPC分类号： G03C5/04 , G01N33/583 , Y10S436/80 , Y10S436/805 , Y10S436/817
摘要： A method for the simultaneous immunochemical assay of trace components in a sample involving competitively reacting the antigens or antibodies in a sample and labeled antigens or labeled antibodies for limited binding sites. The labels are spectral sensitizing dyes. The reaction products are contacted with silver halide and exposed to light having wavelengths corresponding to the absorption spectra of the spectral sensitizing dyes.
摘要翻译：用于同时免疫化学测定样品中痕量组分的方法，其涉及使样品中的抗原或抗体与标记的抗原或标记的抗体竞争性地反应以用于有限的结合位点。标签是光谱增感染料。反应产物与卤化银接触并暴露于具有对应于光谱增感染料的吸收光谱的波长的光。

5. 发明授权

US4861552A Biological reaction layer and its preparation 失效
标题翻译：生物反应层及其制备
公开(公告)号：US4861552A
公开(公告)日：1989-08-29
申请号：US851878
申请日：1986-04-11
申请人： Nobuhito Masuda , Shigeru Nagatomo , Hajime Makiuchi , Yukio Yasuda
发明人： Nobuhito Masuda , Shigeru Nagatomo , Hajime Makiuchi , Yukio Yasuda
IPC分类号： G01N33/52 , C12M1/00 , G01N33/543 , G01N33/78 , C12Q1/00 , G01N33/548
CPC分类号： G01N33/78 , G01N33/54386 , Y10S435/805 , Y10S436/81
摘要： A biological reaction layer of a dry analytical element in the form of a composite porous layer in which a particulate material carrying a biologically active material fixed thereon is dispersed in a fibrous porous matrix, characterized in that the dry weight ratio of said particulate material to said fibrous material contained in said composite porous layer ranges from 1:20 to 1:0.3 and said particulate material is contained therein in the range of 1 to 60 g/m.sup.2.
摘要翻译：复合多孔层形式的干燥分析元件的生物反应层，其中载有固定在其上的生物活性材料的颗粒材料分散在纤维多孔基质中，其特征在于，所述颗粒材料与所述所述复合多孔层中含有的纤维状物质的含量为1:20〜1:0.3，所述粒状物质的含量为1〜60g / m 2。

6. 发明授权

US4404289A Method for immunochemical measurement of trace components 失效
标题翻译：微量元素免疫化学测定方法
公开(公告)号：US4404289A
公开(公告)日：1983-09-13
申请号：US298719
申请日：1981-09-02
申请人： Nobuhito Masuda , Shigeru Nagatomo , Yuji Mihara
发明人： Nobuhito Masuda , Shigeru Nagatomo , Yuji Mihara
IPC分类号： G01N33/58 , G01N33/54 , G01N33/52
CPC分类号： G01N33/583 , Y10S435/805 , Y10S436/805 , Y10S436/807
摘要： In an immunochemical measurement method of an antigen or antibody which comprises competitively reacting an antigen or antibody labelled with spectral sensitizer and an antigen or antibody to be measured, bringing either the reaction product of immune reaction or the unreacted component into contact with silver halide, exposing the same to light having a wavelength which the spectral sensitizer absorbs, developing the exposed silver, and, measuring optical density of the thus formed silver image and/or colored dye, the contact with silver halide is performed in the presence of a specific hydrazine compound. Thus, detection sensitivity is markedly improved.
摘要翻译：在抗原或抗体的免疫化学测定方法中，其包括将标记有光谱敏化剂的抗原或抗体与要测量的抗原或抗体竞争性地反应，使免疫反应的反应产物或未反应的组分与卤化银接触，与具有光谱敏化剂吸收的波长的光相同，曝光的银显影，并且测量由此形成的银图像和/或着色染料的光密度，与卤化银的接触在特定肼化合物的存在下进行。因此，检测灵敏度显着提高。

7. 发明授权

US4337065A Method for immunological assay using multilayer analysis sheet 失效
标题翻译：使用多层分析片的免疫测定方法
公开(公告)号：US4337065A
公开(公告)日：1982-06-29
申请号：US191784
申请日：1980-09-29
申请人： Nobuo Hiratsuka , Yuji Mihara , Nobuhito Masuda , Takushi Miyazako
发明人： Nobuo Hiratsuka , Yuji Mihara , Nobuhito Masuda , Takushi Miyazako
IPC分类号： G03C1/00 , G01N33/536 , G01N33/543 , G01N33/54 , G01N33/52 , G01N33/58
CPC分类号： G01N33/54386
摘要： In a photochemical immunological assay, an antigen or antibody is labelled with a spectral sensitizer or fogging agent for photographic use, and then an immune reaction is caused. The resulting reaction mixture is dropwise added to a multilayer analysis sheet. After either one of the labelled antigen or antibody (F) or the labelled antigen-antibody bound product (B) is brought into contact with silver halide by passing either one through a B/F separating layer provided in the multilayer analysis sheet, the analysis sheet is exposed with a light that the spectral sensitizer or fogging agent absorbs. Then, the resulting optical density obtained by development processing when a spectral sensitizer is used, or without development processing when a fogging agent is used is measured.The multilayer analysis sheet comprises a support having provided thereon a silver halide layer and a B/F separating layer and makes the photochemical immunological assay simpler and more accurate.
摘要翻译：在光化学免疫测定中，抗原或抗体用光谱增感剂或雾化剂标记，用于照相用途，然后引起免疫反应。将所得反应混合物滴加到多层分析片中。将标记的抗原或抗体（F）或标记的抗原 - 抗体结合产物（B）中的任一个通过使任一种通过多层分析片中提供的B / F分离层与卤化银接触，分析片材被光谱增感剂或起雾剂吸收的光暴露。然后，测量当使用光谱增感剂时通过显影处理获得的所得光密度，或者当使用起雾剂时不进行显影处理。多层分析片包括其上设置有卤化银层和B / F分离层的载体，并使光化学免疫测定更简单并且更准确。

8. 发明授权

US4405711A Analysis element for immunochemical measurement of trace components and method for immunochemical measurement using the same 失效
标题翻译：痕量成分的免疫化学测定用分析元素及其免疫化学测定方法
公开(公告)号：US4405711A
公开(公告)日：1983-09-20
申请号：US298812
申请日：1981-09-02
申请人： Nobuhito Masuda , Shigeru Nagatomo , Yuji Mihara
发明人： Nobuhito Masuda , Shigeru Nagatomo , Yuji Mihara
IPC分类号： G01N33/536 , C12Q1/00 , G01N31/22 , G01N33/53 , G01N33/543 , G01N33/58 , G01N33/54 , G01N33/52
CPC分类号： G01N33/583 , G01N33/53 , Y10S435/805 , Y10S435/962 , Y10S436/805 , Y10S436/807
摘要： In a method of assay for a trace component such as antigen, antibody or enzyme utilizing immunochemical reaction or enzyme reaction in combination with photographic detection system comprising measuring optical density of a silver image formed in proportion to the antigen, antibody or enzyme to be measured, a novel analysis sheet comprising a support having provided thereon, in succession, a water absorbing layer and a silver halide emulsion layer is employed. The analysis sheet essentially increases the amount of water absorbed so that the analysis sheet is extremely effective for improving detection sensitivity.
摘要翻译：在利用免疫化学反应或酶反应的微量组分如抗原，抗体或酶的测定方法中，与照相检测系统组合，包括测量与待测量的抗原，抗体或酶成比例形成的银影像的光密度，一种新颖的分析片，其中包括一个其上提供吸水层和卤化银乳剂层的支撑体。分析表基本上增加了吸收的水分量，使分析纸对提高检测灵敏度非常有效。

9. 发明授权

US4337063A Competitive immunoassay using spectral sensitizer label 失效
标题翻译：使用光谱敏化标签进行竞争性免疫测定
公开(公告)号：US4337063A
公开(公告)日：1982-06-29
申请号：US126920
申请日：1980-03-03
申请人： Yuji Mihara , Nobuhito Masuda , Nobuo Hiratsuka , Takushi, both of Miyazako , Tadashi Ikeda
发明人： Yuji Mihara , Nobuhito Masuda , Nobuo Hiratsuka , Takushi, both of Miyazako , Tadashi Ikeda
IPC分类号： G01N33/536 , G01N33/532 , G01N33/537 , G01N33/54 , G01N33/58
CPC分类号： G01N33/537 , G01N33/532 , Y10S436/805 , Y10S436/815 , Y10S436/817
摘要： Method for immunological analysis of a trace component(s) by labelling an antigen or antibody with a marker comprising causing an immune reaction using an antigen or antibody labelled with a spectral sensitizer as a marker, separating the labelled antigen or antibody from the resulting antigen-antibody reaction product, then bringing either the labelled antigen or antibody and the labelled antigen-antibody reaction product into contact with a silver halide, exposing the resulting system to light having a wavelength which the spectral sensitizer absorbs, developing the thus exposed silver halide and then measuring the resulting density.Trace components can thus be photochemically detected and the amount present quantitatively determined. The method provides high reproducibility and high sensitivity comparable to radioimmunoassay but without any risk of radiation exposure.
摘要翻译：通过使用标记抗原或抗体来标记抗原或抗体的免疫学分析方法，所述标记包括使用用光谱增感剂标记的抗原或抗体作为免疫反应作为标记，从得到的抗原 - 然后使标记的抗原或抗体和标记的抗原 - 抗体反应产物与卤化银接触，将所得体系暴露于具有光谱增感剂吸收的波长的光，从而显现出由此暴露的卤化银，然后测量所得密度。因此可以光化学检测痕量成分，定量确定量。该方法提供了与放射免疫测定相当的高重现性和高灵敏度，但没有任何辐射暴露的风险。

10. 发明授权

US4473652A Method and immunochemical measurement 失效
标题翻译：方法和免疫化学测量
公开(公告)号：US4473652A
公开(公告)日：1984-09-25
申请号：US506225
申请日：1983-06-22
申请人： Masaki Okazaki , Nobuhito Masuda , Yoshiro Kumano
发明人： Masaki Okazaki , Nobuhito Masuda , Yoshiro Kumano
IPC分类号： G01N33/58 , G01N33/54
CPC分类号： G01N33/583
摘要： A method for immunochemical assay of an antigen or antibody by labelling the antigen or antibody with a specific cyanine or merocyanine dye containing a carboxy group followed by effecting an immune reaction and photochemical processing thereof is provided. The amount of the antigen or antibody is measured in term of optical density of developed silver halide which is brought into contact with either the antigen-antibody reaction product or the unreacted material.This immunochemical assay method gives high detection sensitivity in a simple operation manner.
摘要翻译：提供了通过用含有羧基的特定花青或部花青染料标记抗原或抗体，然后进行免疫反应和其光化学处理来对抗原或抗体进行免疫化学测定的方法。根据与抗原抗体反应产物或未反应物质接触的显影卤化银的光密度来测量抗原或抗体的量。该免疫化学测定方法以简单的操作方式提供高检测灵敏度。

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