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1. 发明授权

US5019347A Integral multilayer analytical element 失效
标题翻译：整体多层分析元件
公开(公告)号：US5019347A
公开(公告)日：1991-05-28
申请号：US481800
申请日：1990-02-20
申请人： Nobuo Hiratsuka , Asaji Kondo
发明人： Nobuo Hiratsuka , Asaji Kondo
IPC分类号： G01N31/22 , G01N33/52
CPC分类号： G01N33/525 , Y10S435/805
摘要： In an integral multilayer analytical element of a flat sheet type comprising a plurality of layers for quantitative analysis of an analyte in a liquid sample, the improvement which comprises at least two layers of a microporous sheet material arranged adjacent to each other, said at least two layers being combined by an adhesive provided in part on the interface of said layers, whereby the interface allows essentially uniform passage of a liquid therethrough.
摘要翻译：在包括用于液体样品中的分析物的定量分析的多个层的平板型的整体多层分析元件中，所述改进包括彼此相邻布置的至少两层微孔片材料，所述至少两层层通过部分地设置在所述层的界面上的粘合剂组合，由此界面允许液体基本上均匀地通过其中。

2. 发明授权

US4342739A Novel material for immunological assay of biochemical components and a process for the determination of said components 失效
标题翻译：用于生物化学成分的免疫学测定的新型材料和用于测定所述组分的方法
公开(公告)号：US4342739A
公开(公告)日：1982-08-03
申请号：US110318
申请日：1980-01-08
申请人： Fujio Kakimi , Nobuo Hiratsuka , Hiroharu Matsukawa
发明人： Fujio Kakimi , Nobuo Hiratsuka , Hiroharu Matsukawa
IPC分类号： G01N33/543 , G01N33/545 , G01N33/58 , B01J13/02 , G01N33/60
CPC分类号： G01N33/54313 , G01N33/5434 , G01N33/545 , G01N2446/20 , G01N2446/30 , G01N2446/90 , Y10S436/823 , Y10S436/829 , Y10T428/2984 , Y10T428/2985
摘要： Antigen- or antibody-bound microcapsules are employed for immunological assay of biochemical components having immunological activity. The microcapsule system provides high detection sensitivity, high accuracy and excellent reproducibility in immunological assay, without causing non-specific reaction. The microcapsules can be labelled with isotopes, enzymes, etc., for use in quantitative assays of trace amounts of biochemical components.
摘要翻译：抗原或抗体结合的微胶囊用于具有免疫活性的生物化学成分的免疫测定。微胶囊系统在免疫测定中提供高检测灵敏度，高精度和优异的再现性，而不引起非特异性反应。微胶囊可以用同位素，酶等标记，用于微量生化成分的定量测定。

3. 发明授权

US4128470A Supports for electrophoresis and process for the production of the same 失效
标题翻译：支持电泳和生产过程的相同
公开(公告)号：US4128470A
公开(公告)日：1978-12-05
申请号：US879421
申请日：1978-02-21
申请人： Nobuo Hiratsuka , Nakatsugu Yaginuma
发明人： Nobuo Hiratsuka , Nakatsugu Yaginuma
IPC分类号： G01N27/447 , G01N27/26 , G01N27/28
CPC分类号： G01N27/44795 , G01N27/44747
摘要： A support for electrophoresis comprising a porous polymer film having an electroendosmosis factor of 0 to plus about 10 mm, and a process for the production of the support comprising adding a nonionic surface active agent and/or cellulose ether to a mixed solution comprising at least a polymer material for formation of a film and a solvent to produce a porous polymer film.
摘要翻译：一种用于电泳的载体，其包括具有0至+ 10mm的电内渗因子的多孔聚合物膜，以及用于制备所述载体的方法，其包括将非离子表面活性剂和/或纤维素醚加入到至少包含用于形成膜和溶剂的聚合物材料以产生多孔聚合物膜。

4. 发明授权

US5068019A Electrophoresis film support 失效
标题翻译：电泳膜支持
公开(公告)号：US5068019A
公开(公告)日：1991-11-26
申请号：US525963
申请日：1990-05-18
申请人： Takashi Yoshida , Tsueno Kawase , Nobuo Hiratsuka
发明人： Takashi Yoshida , Tsueno Kawase , Nobuo Hiratsuka
IPC分类号： G01N27/447
CPC分类号： G01N27/44747
摘要： An electrophoresis film support particularly useful for the electrophoretic separation of serum proteins. According to one aspect, the electrophoresis film support comprises a microporous polymer membrane sheet containing therein an additive which is selected from the group consisting of hydroxypropyl cellulose, triphenyl phosphate, tricresyl phosphate and mixtures thereof. According to another aspect the electrophoresis film support comprises a microporous membrane sheet made of a polymer material containing therein an additive which is selected from the group consisting of higher fatty acids having 10 to 27 carbon atoms and mixtures thereof, the additive being contained in an amount, based on the weight of the polymer material, ranging from 10 ppm to 3,000 ppm or the maximum solubility limit of the higher fatty acids in the polymer material.

5. 发明授权

US4868131A Competitive binding immunoassay process 失效
标题翻译：竞争结合免疫测定法
公开(公告)号：US4868131A
公开(公告)日：1989-09-19
申请号：US72499
申请日：1987-07-13
申请人： Nobuo Hiratsuka
发明人： Nobuo Hiratsuka
IPC分类号： G01N33/536 , G01N33/538 , G01N33/543
CPC分类号： G01N33/538 , G01N33/54313 , G01N33/54386
摘要： An immunoassay process for quantitative analysis of an analyte antigen (or analyte antibody) in an aqueous liquid sample. In the first step, the analyte antigen (or analyte antibody) is allowed to react with an antibody (or antigen) in competition with a known quantity of a labelled antigen (or labelled antibody), the antibody (or antigen) being carried by water-insoluble microparticles in the immobilized state. In the second step, the reaction mixture is spotted on a top porous layer of an integral multi-layered analysis element which includes the top porous layer, an intermediate detection layer and a bottom water-impermeable and transparent support. The top porous layer permeates aqueous solution and has pores sufficiently small to trap all of the microparticles carrying the antibodies (or antigens) bound to the analyte antigen (or analyte antibody) and bound to the labelled antigen (or labelled antibody). Thus, the free labelled antigen is allowed to pass through the top porous layer and migrate to the detection layer. In the third step, the quantity of the marker labelling to the labelled antigen (or labelled antibody) which has not been bound to the immobilized antibody (immobilized antigen) is determined.
摘要翻译：用于定量分析含水液体样品中的分析物抗原（或分析物抗体）的免疫测定方法。在第一步中，使分析物抗原（或分析物抗体）与已知量的标记抗原（或标记的抗体）竞争的抗体（或抗原）反应，抗体（或抗原）由水携带不溶性微粒处于固定状态。在第二步骤中，将反应混合物点在包括顶部多孔层，中间检测层和底部不透水透明载体的整体多层分析元件的顶部多孔层上。顶部多孔层渗透水溶液并且具有足够小的孔以捕获携带与分析物抗原（或分析物抗体）结合并结合到标记抗原（或标记抗体）的抗体（或抗原）的所有微粒。因此，允许游离标记的抗原通过顶部多孔层并迁移到检测层。在第三步骤中，确定标记为未结合固定化抗体（固定化抗原）的标记抗原（或标记抗体）的标记物的量。

6. 发明授权

US4753531A Flat container type analytical instrument 失效
标题翻译：扁平容器式分析仪
公开(公告)号：US4753531A
公开(公告)日：1988-06-28
申请号：US873284
申请日：1986-06-04
申请人： Nobuo Hiratsuka , Asaji Kondo
发明人： Nobuo Hiratsuka , Asaji Kondo
IPC分类号： B01L3/00 , G01N21/78 , G01N21/01 , G01N1/10
CPC分类号： B01L3/5023 , G01N21/78 , B01L2300/0816 , B01L2400/0406 , B01L2400/0481
摘要： An integral flat container type analytical instrument for use in the quantitative analysis of an analyte present in a liquid sample which has a reaction chamber and an observation chamber associated with each other so as to allow a liquid to flow therebetween characterized in that: at least part of the wall of said reaction chamber is made of a flexible material; said observation chamber is provided with wall parts which are made of a transparent material and opposed to each other in such a manner that their relative positions are fixed to provide a given light path; and no partition wall preventing the free flow of the liquid substantially exists between said reaction chamber and said observation chamber. A method for optical measurement of an analyte in a liquid sample utilizing said analytical instrument is also disclosed.
摘要翻译：一种用于定量分析存在于液体样品中的分析仪的一体式扁平容器型分析仪器，其具有彼此相关联的反应室和观察室，以使液体在其间流动，其特征在于：至少部分所述反应室的壁由柔性材料制成; 所述观察室设置有由透明材料制成并且彼此相对的壁部分，使得它们的相对位置被固定以提供给定的光路; 并且在所述反应室和所述观察室之间基本上不存在防止液体自由流动的分隔壁。还公开了利用所述分析仪器对液体样品中的分析物进行光学测量的方法。

7. 发明授权

US4429050A Competitive immunochemical measurement of plural trace components involving spectral sensitizing dye labels 失效
标题翻译：涉及光谱增感染料标签的多种微量成分的竞争性免疫化学测定
公开(公告)号：US4429050A
公开(公告)日：1984-01-31
申请号：US298811
申请日：1981-09-02
申请人： Yukio Yasuda , Nobuhito Masuda , Yuji Mihara , Masaki Okazaki , Nobuo Hiratsuka
发明人： Yukio Yasuda , Nobuhito Masuda , Yuji Mihara , Masaki Okazaki , Nobuo Hiratsuka
IPC分类号： G03C5/00 , G01N33/532 , G01N33/536 , G01N33/58 , G03C1/12 , G03C5/04 , G01N33/54 , G01N33/52 , G01N33/74
CPC分类号： G03C5/04 , G01N33/583 , Y10S436/80 , Y10S436/805 , Y10S436/817
摘要： A method for the simultaneous immunochemical assay of trace components in a sample involving competitively reacting the antigens or antibodies in a sample and labeled antigens or labeled antibodies for limited binding sites. The labels are spectral sensitizing dyes. The reaction products are contacted with silver halide and exposed to light having wavelengths corresponding to the absorption spectra of the spectral sensitizing dyes.
摘要翻译：用于同时免疫化学测定样品中痕量组分的方法，其涉及使样品中的抗原或抗体与标记的抗原或标记的抗体竞争性地反应以用于有限的结合位点。标签是光谱增感染料。反应产物与卤化银接触并暴露于具有对应于光谱增感染料的吸收光谱的波长的光。

8. 发明授权

US4331444A Competitive immunoassay using silver halide fogging agent 失效
标题翻译：使用卤化银雾化剂进行竞争性免疫测定
公开(公告)号：US4331444A
公开(公告)日：1982-05-25
申请号：US126919
申请日：1980-03-03
申请人： Yuji Mihara , Nobuhito Masuda , Nobuo Hiratsuka , Takushi Miyazako , Shigeo Hirano
发明人： Yuji Mihara , Nobuhito Masuda , Nobuo Hiratsuka , Takushi Miyazako , Shigeo Hirano
IPC分类号： G01N33/536 , G01N33/537 , G01N33/54 , G01N33/58
CPC分类号： G01N33/537 , Y10S436/805
摘要： In a method for the immunological analysis of trace components by marking or labelling an antigen or antibody with a marker, an immune reaction is caused using an antigen or antibody marked with a fogging agent for silver halide, the labelled antigen or antibody is separated from the labelled antigen-antibody reaction product, the silver halide is developed in the presence of either one of the labelled antigen or antibody and the labelled antigen-antibody reaction product, and the density obtained is measured.The method is comparable to radioimmunoassay in having high reproductibility and sufficient sensitivity but does not involve any risk due to radiation.
摘要翻译：在通过用标记物标记或标记抗原或抗体来对痕量组分的免疫分析的方法中，使用用卤化银的起雾剂标记的抗原或抗体引起免疫反应，将标记的抗原或抗体从标记的抗原 - 抗体反应产物，在标记的抗原或抗体之一和标记的抗原 - 抗体反应产物的存在下显影卤化银，并测量获得的密度。该方法与放射免疫测定相当，具有高的可再生性和足够的灵敏度，但不涉及由于辐射引起的任何风险。

9. 发明授权

US4097383A Process for production of microporous sheet 失效
标题翻译：微孔板生产工艺
公开(公告)号：US4097383A
公开(公告)日：1978-06-27
申请号：US777481
申请日：1977-03-14
申请人： Sumio Ohtani , Nobuo Hiratsuka , Masaru Horiguchi
发明人： Sumio Ohtani , Nobuo Hiratsuka , Masaru Horiguchi
IPC分类号： B01D39/14 , B01D39/16 , B29C67/20 , B29D7/01 , B32B31/18
CPC分类号： B01D69/10 , B01D39/1692 , B01D67/00 , B01D69/00 , B01D69/141 , B29C67/20 , Y10S264/47 , Y10S264/48 , Y10T156/1059 , Y10T156/1168 , Y10T428/249961 , Y10T428/249978
摘要： A process for producing a microporous sheet comprising peeling off a microporous sheet of a resin bonded to a plate from the plate in such a manner that the microporous sheet is split into two sheets.
摘要翻译：一种微孔片的制造方法，其特征在于，将所述微孔片分割成两片，从所述板剥离与板接合的树脂的微孔片。

10. 发明授权

US4171987A Micro-porous sheet 失效
标题翻译：微孔片
公开(公告)号：US4171987A
公开(公告)日：1979-10-23
申请号：US558099
申请日：1975-03-13
申请人： Masaru Horiguchi , Nobuo Hiratsuka , Sumio Otani
发明人： Masaru Horiguchi , Nobuo Hiratsuka , Sumio Otani
IPC分类号： B01D39/18 , C08J9/28 , C08L1/10 , C08L1/12 , G01N27/447
CPC分类号： B01D71/16 , B01D69/02 , B01D71/18 , B01D71/20 , B01D71/22 , C08L1/10 , G01N27/44747
摘要： A micro-porous sheet having excellent thermostability which contains a cellulose ester as a basic component and 0.1 to 20 wt% of a cellulose ether based on the cellulose ester.
摘要翻译：一种具有优异的热稳定性的微孔片，其含有纤维素酯作为碱性成分，0.1〜20重量％的纤维素醚基于纤维素酯。

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