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1. 发明申请

US20060191848A1 Integrated separation of organic substances from an aqueous bio-process mixture 审中-公开
标题翻译：有机物质与水生物过程混合物的综合分离
公开(公告)号：US20060191848A1
公开(公告)日：2006-08-31
申请号：US10513894
申请日：2003-05-02
申请人： Nicole Ruffer , Christian Wandrey , Ralf Takors
发明人： Nicole Ruffer , Christian Wandrey , Ralf Takors
IPC分类号： C02F9/00
CPC分类号： B01D11/048 , B01D11/0488
摘要： The present invention relates to a process for integrated removal of one or more organic substances present in an aqueous bioprocess mixture, which comprise at least one positively charged and/or chargeable nitrogen-containing group, by means of reactive extraction in at least one step, in which process at least one liquid-liquid centrifuge is used for said reactive extraction and said organic substances are re-extracted from the extractant into an aqueous phase. More specifically, the bioprocess mixture directed to the liquid-liquid centrifuges is rendered cell-free and protein-free, before being directed into the first centrifuge. Furthermore, the invention relates to such a process in which an aqueous bioprocess mixture is continuously removed from a bioreactor, led, with an extractant, into a liquid-liquid centrifuge, extracted by means of said extractant, with an organic phase being obtained which contains the substance to be extracted from the fermentation mixture. Said substance may be re-extracted in a cycle via a second liquid-liquid centrifuge, resulting subsequently in a concentrated aqueous solution of the extracted substance, and the organic phase being recycled into the first liquid-liquid centrifuge.
摘要翻译：本发明涉及通过在至少一个步骤中的反应性萃取来整合去除存在于含水生物过程混合物中的一种或多种有机物质的方法，所述含水生物过程混合物包含至少一个带正电荷和/或带电荷的含氮基团，在该方法中，使用至少一种液 - 液离心机用于所述反应萃取，并且将所述有机物质从萃取剂中再萃取到水相中。更具体地，在被引导到第一离心机之前，被引导到液体 - 液体离心机的生物过程混合物被赋予无细胞和无蛋白质。此外，本发明涉及这样的方法，其中将水性生物过程混合物从生物反应器中连续地从生物反应器中取出，并用萃取剂引入到液体离心机中，通过所述萃取剂提取，得到的有机相包含从发酵混合物中提取的物质。所述物质可以经由第二液 - 液离心机在循环中再提取，随后产生萃取物质的浓缩水溶液，并将有机相再循环到第一液 - 液离心机中。

2. 发明授权

US06793825B2 Process for the separation of organic substances from an aqueous mixture 失效
标题翻译：从含水混合物中分离有机物质的方法
公开(公告)号：US06793825B2
公开(公告)日：2004-09-21
申请号：US09984290
申请日：2001-10-29
申请人： Dietrich H. Maass , Dirk Weuster-Botz , Ralf Takors , Christian Wandrey , Holger Paschold
发明人： Dietrich H. Maass , Dirk Weuster-Botz , Ralf Takors , Christian Wandrey , Holger Paschold
IPC分类号： C02F144
CPC分类号： B01D61/246 , C07B63/00 , C07C227/40
摘要： The present invention relates to a process for separating from an aqueous mixture one or more organic substances containing at least one positively charged and/or chargeable nitrogenous group by means of extraction via at least one porous membrane, wherein use is made of an extraction agent which contains at least partially relatively long-chain organic compounds and at least one liquid cation exchanger, and of a membrane that is wettable by either the aqueous mixture or by the extraction agent. In particular, the invention relates to a process for separating from an aqueous mixture one or more organic substances containing at least one positively charged and/or chargeable nitrogenous group by means of extraction via at least one porous membrane, wherein the aqueous mixture is drawn from a reservoir 1, led across a first porous membrane 3 which is wettable by either the aqueous mixture or by an extraction agent 5 which contains at least partially relatively long-chain organic compounds and at least one liquid cation exchanger, extracted with the extraction agent 5, the aqueous retentate is returned to the reservoir, the extracted organic substances are led across a second porous membrane 6 which is wettable by either the aqueous mixture or by the extraction agent and there re-extracted into an aqueous phase 7.
摘要翻译：本发明涉及一种通过至少一种多孔膜从含水混合物中分离含有至少一个带正电和/或带电荷的含氮基团的有机物质的方法，其中使用提取剂含有至少部分相对较长链的有机化合物和至少一种液体阳离子交换剂，以及可由含水混合物或萃取剂润湿的膜。特别地，本发明涉及一种通过至少一种多孔膜从含水混合物中分离含有至少一个带正电和/或带电荷的含氮基团的有机物质的方法，其中将含水混合物从一个储存器1，穿过第一多孔膜3，该第一多孔膜3可由含水混合物或提取剂5润湿，该提取剂含有至少部分相对较长链的有机化合物和至少一种液体阳离子交换剂，用萃取剂5萃取，水性渗余物返回储存器，萃取的有机物质被引导通过第二多孔膜6，第二多孔膜6可由含水混合物或萃取剂润湿，并再次萃取到水相7中。

3. 发明申请

US20050227333A1 Process for the production of an aromatic amino acid metabolite or derivative thereof 审中-公开
标题翻译：用于生产芳香族氨基酸代谢物或其衍生物的方法
公开(公告)号：US20050227333A1
公开(公告)日：2005-10-13
申请号：US10497587
申请日：2002-12-05
申请人： Marc Gerigk , Leon Raeven , Georg Sprenger , Ralf Takors , Christian Wandrey
发明人： Marc Gerigk , Leon Raeven , Georg Sprenger , Ralf Takors , Christian Wandrey
IPC分类号： C12M1/36 , C12P7/22 , C12P13/22 , C12Q3/00
CPC分类号： C12P7/22 , C12P13/22
摘要： The invention relates to a process for the production of an aromatic amino acid metabolite or derivative thereof by aerobic fermentation of Escherichia coli, which fermentation comprises a growth and a production phase and in which fermentation glucose and L-tyrosine are controlled, wherein during at least part of the production phase, the glucose concentration in the fermentation medium is controlled within the range of 1-20 g/L and the L-tyrosine concentration in the fermentation medium is controlled below 36 mg/L.
摘要翻译：本发明涉及通过大肠杆菌的好氧发酵生产芳香族氨基酸代谢物或其衍生物的方法，该发酵包括生长和生产阶段，其中控制发酵葡萄糖和L-酪氨酸，其中至少部分生产阶段，发酵培养基中的葡萄糖浓度控制在1-20g / L的范围内，发酵培养基中的L-酪氨酸浓度控制在36mg / L以下。

4. 发明授权

US06303351B1 Process for the continuous production of citric acid by fermentation 失效
标题翻译：通过发酵连续生产柠檬酸的方法
公开(公告)号：US06303351B1
公开(公告)日：2001-10-16
申请号：US08208123
申请日：1994-03-08
申请人： Savas Anastassiadis , Alexander Aivasidis , Christian Wandrey
发明人： Savas Anastassiadis , Alexander Aivasidis , Christian Wandrey
IPC分类号： C12N744
CPC分类号： C12P7/48 , C07C59/265
摘要： A process for producing citric acid/citrate for use in producing citric acid, comprising the steps of continuously feeding to a fermenter containing a yeast capable of transforming glucose to citric acid by fermentation, in the presence of a nutrient medium and oxygen, sugar, especially glucose as a C source and at least one ammoniun compound as an N source fermenting the sugar to citric acid in said fermenter at a temperature, a pH and with a residence time sufficient to transform at least a major part of the sugar continuously fed to said fermenter to citric acid continuously withdrawing fermentation product from said fermenter recovering citric acid/citrate from said fermentation product controlling a C/N ratio fed to said fermenter to correspond to a molar ratio of glucose to ammonium compounds of 12 to 22 during fermentation of the glucose to citrate.
摘要翻译：一种生产用于生产柠檬酸的柠檬酸/柠檬酸盐的方法，包括以下步骤：在营养培养基和氧气，糖，特别是存在下，连续喂食含有能够通过发酵将葡萄糖转化为柠檬酸的酵母的发酵罐葡萄糖作为C源和至少一种作为N源的铵化合物，在所述发酵罐中，在温度，pH和停留时间内将糖发酵成柠檬酸，并且具有足以将至少大部分连续供给至所述发酵罐至柠檬酸连续地从所述发酵罐抽出发酵产物，从所述发酵产物回收柠檬酸/柠檬酸，控制进料至所述发酵罐的C / N比值，以对应葡萄糖发酵期间葡萄糖与铵化合物的摩尔比为12至22 到柠檬酸盐。

5. 发明授权

US5795750A Process for the continuous enzymatic extraction of hydrophobic products and device suitable therefor 失效
标题翻译：用于连续酶提取疏水产品的方法和适合的装置
公开(公告)号：US5795750A
公开(公告)日：1998-08-18
申请号：US826881
申请日：1997-04-07
申请人： Wolfgang Kruse , Udo Kragl , Christian Wandrey
发明人： Wolfgang Kruse , Udo Kragl , Christian Wandrey
IPC分类号： C12M1/40 , C12P1/00 , C12P7/04 , C12P7/22 , C12P7/26 , C12P13/00 , C12M1/12 , C12N11/00 , C12P7/64
CPC分类号： C12P1/00 , C12M21/18 , C12M29/04 , C12M29/18 , C12P7/04 , C12P7/26 , Y02P20/582 , Y10S435/813 , Y10S435/817
摘要： In a process for the continuous enzyme-catalyticzed enzymatic production of hydrophobic products in an aqueous solution of a reaction mixture, the product is extracted from the product containing reaction mixture by way of a product-permeable microporous membrane into an organic solvent and the product-depleted reaction mixture is recycled.
摘要翻译：在反应混合物的水溶液中连续酶催化生产疏水产物的方法中，通过产物渗透微孔膜将产物从含产物的反应混合物中提取到有机溶剂中，贫化的反应混合物被回收。

6. 发明授权

US5130026A Process and apparatus for removing ammonium from aqueous liquids 失效
标题翻译：从水性液体中除去氨的方法和装置
公开(公告)号：US5130026A
公开(公告)日：1992-07-14
申请号：US705655
申请日：1991-05-24
申请人： Manfred Biselli , Joerg Thoemmes , Christian Wandrey
发明人： Manfred Biselli , Joerg Thoemmes , Christian Wandrey
IPC分类号： B01D15/04 , B01D61/24 , B01J47/12 , C01C1/02 , C02F1/469 , C02F1/58 , C12M1/12 , C12M3/06
CPC分类号： B01D61/243 , C12M29/04 , C12M41/26 , C12M47/10
摘要： A process for the removal of ammonium from aqueous liquids, especially from fermentation broths, is disclosed. The process uses a cation exchanger membrane which separates a liquid on a donor side that is to be depleted of NH.sub.4.sup.+ from a solution on an acceptor side whose pH is higher than on the donor side. Because of the equilibriumNH.sub.4.sup.+ .revreaction.NH.sub.3 +H+there is on the acceptor side increased formation of NH.sub.3. This is continuously removed, in particular by reducing the pressure and/or passing through inert gas, or by selective transport through another microporous membrane into an acid protonation solution, so that a NH.sub.4.sup.+ concentration gradient is maintained across the membrane. A similar content, apart from the ammonium concentration, of concomitant cations on the donor and acceptor side and/or a high ratio of the solution volumes on the donor side and the acceptor side ensures that the solution on the donor side to be depleted of NH.sub.4.sup.+, does not lose concomitant ions. A suitable apparatus comprises a flow section which can be coupled into a bypass and has a cation exchanger limiting membrane which is simultaneously a limiting wall of an acceptor chamber. The acceptor chamber may contain devices for pH control and NH.sub.3 removal or these may be contained in a flow section.
摘要翻译：公开了从含水液体，特别是发酵液中除去铵的方法。该方法使用阳离子交换膜，其将待排出NH 4 +的供体侧上的液体从pH高于供体侧的受体侧上的溶液分离。由于平衡NH4 + - > < - NH3 + H +在受体侧上增加了NH3的形成。这是连续去除的，特别是通过降低压力和/或通过惰性气体，或通过选择性地将另一个微孔膜输送到酸性质子溶液中，使得横跨该膜保持NH 4 +浓度梯度。供体和受体侧上伴随的阳离子和/或供体侧和受体侧的溶液体积的高比例，除了铵浓度以外，类似的含量确保供体侧的溶液被耗尽NH4 + ，不会伴随离子丢失。合适的装置包括流动部分，其可以耦合到旁路中并且具有同时是受体室的限制壁的阳离子交换器限制膜。受体室可以包含用于pH控制和NH 3去除的装置，或者这些装置可以包含在流动部分中。

7. 发明授权

US06355453B1 Method for making fluorinated sugars having a side chain and use thereof 失效
标题翻译：制造具有侧链的氟化糖及其用途的方法
公开(公告)号：US06355453B1
公开(公告)日：2002-03-12
申请号：US09483548
申请日：2000-01-14
申请人： Juliane Beliczey , Udo Kragl , Andreas Liese , Christian Wandrey , Kurt Hamacher , Heinz Hubert Coenen , Thomas Tierling
发明人： Juliane Beliczey , Udo Kragl , Andreas Liese , Christian Wandrey , Kurt Hamacher , Heinz Hubert Coenen , Thomas Tierling
IPC分类号： C12P1926
CPC分类号： C12P19/26
摘要： Furanosides or pyranosides can be reacted with pyruvate and converted into sialic acids in good yields by an enzymatic synthesis, using a suitable aldolase, where the concentration of aldolase, per 50 mM of pyruvate, is 1 to 2,500 U*/ml. For example, 3-fluoroneuraminic acid and other 3,3-didesoxy-3-fluorononulopyranosonic acid derivatives can be prepared in this manner. The F-atom in the resulting sialic acid (which can be 19F or 19F and 18F) provides a label which makes possible studies of mammalian physiology and diagnosis of mammalian diseases.
摘要翻译：呋喃糖苷或吡喃糖苷可以与丙酮酸反应，并通过酶合成，以合适的醛缩酶将其转化成唾液酸，其中醛糖醛酸的浓度为每50mM丙酮酸盐为1至2,500U / ml。例如，可以以这种方式制备3-氟尿神经氨酸和其它的3,3-didesoxy-3-fluorononuloproranosonic acid衍生物。所得唾液酸（其可以是19F或19F和18F）中的F原子提供了可以研究哺乳动物生理学和哺乳动物疾病诊断的标签。

8. 发明授权

US5962286A Process for the production of gluconic acid with a strain of Aureobasidium pullulans (de bary) Arnaud 失效
标题翻译：生产葡萄糖酸的方法与挥发性有芽芽孢杆菌（deure）Arnaud
公开(公告)号：US5962286A
公开(公告)日：1999-10-05
申请号：US784076
申请日：1997-01-17
申请人： Savas Anastassiadis , Alexander Aivasidis , Christian Wandrey
发明人： Savas Anastassiadis , Alexander Aivasidis , Christian Wandrey
IPC分类号： C12P7/42 , C12P19/02 , C12N1/00 , C12N1/02 , C12P1/02
CPC分类号： C12R1/645 , C12P19/02 , C12P7/42 , Y10S435/822
摘要： Strains of Aureobasidium pullulans (de bary) Arnaud can be used for the commercial production of gluconic acid by fermentation in aqueous liquid containing sugar, which in continuous culture from glucose form greater than or equal to 90% and greater than or equal to 90% molar selectivity. The process is conducted with an Fe and Mn optimized medium with a nitrogen-independent (N-independent) ion concentration. The iron (Fe) concentration with 3 g/l NH.sub.4 Cl is greater than or equal to 0.5 mM, and the manganese (Mn) concentration with 3 g/l NH.sub.4 Cl is greater than or equal to about 0.5 mM manganese (Mn). The pH is regulated between about 4.5 and about 8, in particular at 6.5-7, and the temperature between 24 and 32.degree. C., in particular at 29-30.degree. C. Particularly successful strains are Aureobasidium pullulans (de bary) Arnaud with the registration numbers DSM 7085, DSM 7086, DSM 7087, and DSM 7088.
摘要翻译： of of）））））））））molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar molar 选择性。该过程用具有氮独立（N非依赖）离子浓度的Fe和Mn优化的介质进行。用3g / l NH 4 Cl的铁（Fe）浓度大于或等于0.5mM，3g / l NH 4 Cl的锰（Mn）浓度大于或等于约0.5mM锰（Mn）。 pH调节在约4.5至约8之间，特别是6.5-7，温度在24至32℃之间，特别是在29-30℃。特别成功的菌株是挥发油芽孢杆菌（deutary）Arnaud，注册号DSM 7085，DSM 7086，DSM 7087和DSM 7088。

9. 发明授权

US5369016A Peptide amidase and the use thereof 失效
标题翻译：肽酰胺酶及其用途
公开(公告)号：US5369016A
公开(公告)日：1994-11-29
申请号：US005819
申请日：1993-01-19
申请人： Doerte Steinke , Maria-Regina Kula , Alexander Schwarz , Christian Wandrey
发明人： Doerte Steinke , Maria-Regina Kula , Alexander Schwarz , Christian Wandrey
IPC分类号： C07K1/107 , C12N9/78 , C12N9/80 , C12P13/04 , C12P21/00 , C12P21/02
CPC分类号： C07K1/107 , C12N9/80
摘要： A peptide amidase isolated from the flavedo of citrus fruits, preferably oranges, which is capable of catalyzing the selective hydrolytic elimination of the free amino group on the C-terminal end of peptide amides but which does not cleave peptide bonds. The enzyme accepts D-amino acid residues in the C-terminal position, although the hydrolysis rate is much slower than with L-amino acid residues. The enzyme is weakly inhibited by serine protease inhibitors; has an optimal pH of 7.5.+-.1.5, an optimum temperature of 30.degree. C. at pH 7.5 and has an isoelectric point of pH 9.5. The peptide amidase is stable at pH 6.0-9.0. The molecular weight of the purified enzyme is 32,000.+-.3000 daltons. A peptide amidase according to the present invention is particularly useful in the production of peptides by continuous enzymatic reaction of N-protected amino acid or peptide alkyl esters with amides of amino acids. In the continuous reaction, the synthesized peptide amide is hydrolyzed by the peptide amidase and separated by anion exchange from the amide of the amino acid which can be recycled.
摘要翻译：从柑橘类水果（优选橙子）中分离的肽酰胺酶，其能够催化肽酰胺的C末端上的游离氨基的选择性水解消除，但不切割肽键。该酶在C-末端位置接受D-氨基酸残基，尽管水解速度比L-氨基酸残基慢得多。酶被丝氨酸蛋白酶抑制剂弱抑制; 最佳pH为7.5 +/- 1.5，最适温度为30℃，pH 7.5，等电点pH值为9.5。肽酰胺酶在pH 6.0-9.0下是稳定的。纯化酶的分子量为32,000 +/- 3000道尔顿。根据本发明的肽酰胺酶特别可用于通过N-保护的氨基酸或肽烷基酯与氨基酸酰胺的连续酶反应来生产肽。在连续反应中，合成的肽酰胺被肽酰胺酶水解，并通过阴离子交换从可再循环的氨基酸的酰胺中分离。

10. 发明授权

US5200326A Method for the fermentative production of L-amino acids from .alpha.-keto acids 失效
标题翻译：从（ALPHA） - 酮酸生成L-氨基酸的方法
公开(公告)号：US5200326A
公开(公告)日：1993-04-06
申请号：US44678
申请日：1987-05-01
申请人： Christian Wandrey , Rolf Wichmann , Ulrich Groeger , Manfred Kircher , Wolfgang Leuchtenberger , Eberhard Breuker
发明人： Christian Wandrey , Rolf Wichmann , Ulrich Groeger , Manfred Kircher , Wolfgang Leuchtenberger , Eberhard Breuker
IPC分类号： C12P13/04 , C12P13/06 , C12R1/13 , C12R1/15
CPC分类号： C12P13/06 , C12P13/04 , Y10S435/84 , Y10S435/843
摘要： The invention is directed to a method of producing L-amino acids from .alpha.-ketocarboxylic acids by means of continuous fermentation with the aid of glutamate producing bacteria with biomass retention in which the cells are separated by microfiltration and/or centrifugal separators.
摘要翻译：本发明涉及一种通过在具有生物质保留的谷氨酸生产细菌的帮助下通过连续发酵从α-酮羧酸生产L-氨基酸的方法，其中通过微量过滤和/或离心分离器分离细胞。

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