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1. 发明申请

US20190071735A1 TARGET ANALYSIS METHOD AND TARGET ANALYSIS KIT FOR USE IN THE METHOD 审中-公开
公开(公告)号：US20190071735A1
公开(公告)日：2019-03-07
申请号：US16071977
申请日：2017-01-20
申请人： NEC Solution Innovators, Ltd.
发明人： Yoshihito YOSHIDA , Katsunori HORII , Jou AKITOMI , Naoto KANEKO , Akihisa SHIMIZU , Tomoko FUJITA , Iwao WAGA
IPC分类号： C12Q1/6895 , C12Q1/66 , G01N33/02 , G01N33/00 , G01N33/543
摘要： The present invention provides a new target analysis method and a target analysis kit for use in the method. The target analysis method of the present invention includes: causing a specimen, a labeled binding nucleic acid molecule that binds to a target, and a carrier on which a blocking nucleic acid molecule that binds to the labeled binding nucleic acid molecule is immobilized to react; separating a fraction of the carrier and a fraction of components other than the carrier from each other; and detecting a label of the labeled binding nucleic acid molecule in at least one of the fraction of the carrier and the fraction of components other than the carrier to analyze a target in the specimen.

2. 发明申请

US20160003811A1 NUCLEIC ACID ELEMENT CANDIDATE MOLECULE AND SCREENING METHOD FOR SCREENING FOR NUCLEIC ACID ELEMENT FOR TARGET ANALYSIS USING THE SAME 有权
标题翻译：用于筛选用于目标分析的核酸元素的核酸元件候选分子和筛选方法
公开(公告)号：US20160003811A1
公开(公告)日：2016-01-07
申请号：US14770520
申请日：2014-02-17
申请人： NEC SOLUTION INNOVATORS, LTD.
发明人： Jou AKITOMI , Naoto KANEKO , Katsunori HORII
IPC分类号： G01N33/53
CPC分类号： G01N33/5308 , C12Q1/6816 , C12Q2521/337 , C12Q2521/345
摘要： The present invention provides a novel nucleic acid element candidate molecule for use in screening for a nucleic acid element for target analysis and a novel screening method for screening for a nucleic acid element for target analysis using the same. The candidate molecule according to the present invention is a molecule for screening for a nucleic acid element for target analysis, being the following single-stranded nucleic acid (I): (I) a single-stranded nucleic acid comprising a catalyst sequence (D), a blocking sequence (B), and a binding sequence (A) that binds to a target, linked in this order. The blocking sequence (B) is complementary to a partial region (Dp) in the catalyst sequence (D). A terminal region (Ab) on the blocking sequence (B) side in the binding sequence (A) is complementary to a flanking region (Df) of the partial region (Dp) in the catalyst sequence (D) and is complementary to a terminal region (Af) on the side opposite to the blocking sequence (B) side in the binding sequence (A).
摘要翻译：本发明提供了用于筛选用于靶分析的核酸元件的新型核酸元件候选分子和用于筛选使用其的靶分析的核酸元件的新型筛选方法。根据本发明的候选分子是用于筛选用于靶分析的核酸元件的分子，是以下单链核酸（I）：（I）包含催化剂序列（D）的单链核酸，，阻断序列（B）和与目标结合的结合序列（A）。阻断序列（B）与催化剂序列（D）中的部分区域（Dp）互补。结合序列（A）中的阻断序列（B）侧的末端区域（Ab）与催化剂序列（D）中的部分区域（Dp）的侧翼区域（Df）互补，并且与末端区域（Af）在与结合序列（A）中的阻断序列（B）侧相反的一侧。

3. 发明申请

US20210187499A1 TARGET ANALYSIS KIT AND TARGET ANALYSIS METHOD 有权
公开(公告)号：US20210187499A1
公开(公告)日：2021-06-24
申请号：US16318951
申请日：2017-03-23
申请人： NEC SOLUTION INNOVATORS LTD.
发明人： Yoshihito YOSHIDA , Katsunori HORII , Iwao WAGA , Jou AKITOMI , Naoto KANEKO
IPC分类号： B01L3/00 , C12Q1/6813 , G01N33/543
摘要： A target analysis kit includes: a sample holder including: a reagent chamber containing a first reagent; a sample holding portion; and a liquid transfer portion; and an analysis container including: a first chamber containing a second reagent; and a second chamber with a labeling substance contained in the first or second reagent to be detected. A combination of the first and second reagents is any of the combinations (1) to (3) and (4): (1) first reagent is the immobilized second binding substance and second reagent is the labeled first binding substance; (2) first reagent is the labeled first binding substance and second reagent is the immobilized second binding substance; (3) first reagent is the immobilized first binding substance and second reagent is the labeled second binding substance; and (4) first reagent is the labeled second binding substance and second reagent is the immobilized first binding substance.

4. 发明申请

US20160202154A1 METHOD FOR PRODUCING SAMPLE AND METHOD FOR ANALYZING TARGET 审中-公开
标题翻译：生产样本的方法和分析目标的方法
公开(公告)号：US20160202154A1
公开(公告)日：2016-07-14
申请号：US14916942
申请日：2014-06-27
申请人： NEC SOLUTION INNOVATORS, LTD.
发明人： Katsunori HORII , Jou AKITOMI , Naoto KANEKO , Iwao WAGA
IPC分类号： G01N1/30 , G01N33/538 , G01N33/04 , G01N33/53
CPC分类号： G01N1/30 , C12N15/113 , C12N15/115 , C12N2310/127 , C12N2310/16 , C12N2310/3519 , C12Q1/6806 , G01N30/88 , G01N30/96 , G01N33/04 , G01N33/5308 , G01N33/538 , G01N33/566 , G01N2030/8813 , C12Q2527/125
摘要： The present invention is intended to provide a novel sensor for target analysis and a target analysis method using the same.The sensor for target analysis according to the present invention includes a single-stranded nucleic acid molecule. The single-stranded nucleic acid molecule includes a first catalytic nucleic acid region (D1), a second catalytic nucleic acid region (D2), and a binding nucleic acid region (Ap) that binds to a target. The single-stranded nucleic acid molecule includes the first catalytic nucleic acid region (D1) at one end of the binding nucleic acid region (Ap) and the second catalytic nucleic acid region (D2) at the other end of the binding nucleic acid (Ap). In the absence of a target, the catalytic function by the first catalytic nucleic acid region (D1) and the second catalytic nucleic acid region (D2) is inhibited. In the presence of a target, the catalytic function is generated owing to formation of a G-quartet of the first catalytic nucleic acid region (D1) and the second catalytic nucleic acid region (D2) due to the contact between the target and the binding nucleic acid region (Ap).
摘要翻译：本发明旨在提供一种用于目标分析的新型传感器和使用其的目标分析方法。根据本发明的用于靶分析的传感器包括单链核酸分子。单链核酸分子包括与靶标结合的第一催化核酸区域（D1），第二催化核酸区域（D2）和结合核酸区域（Ap）。单链核酸分子包括在结合核酸区（Ap）一端的第一催化核酸区（D1）和结合核酸另一端的第二催化核酸区（D2））。在没有靶的情况下，抑制了第一催化核酸区（D1）和第二催化核酸区（D2）的催化功能。在靶的存在下，由于靶和结合物之间的接触，由于形成第一催化核酸区域（D1）和第二催化核酸区域（D2）的G四重奏，产生催化功能核酸区（Ap）。

5. 发明申请

US20160146772A1 FLUORESCENSE SENSOR FOR TARGET ANALYSIS, KIT FOR TARGET ANALYSIS, AND TARGET ANALYSIS METHOD USING SAME 有权
标题翻译：用于目标分析的荧光传感器，目标分析工具箱和使用相同目标的目标分析方法
公开(公告)号：US20160146772A1
公开(公告)日：2016-05-26
申请号：US14906897
申请日：2014-06-27
申请人： NEC SOLUTION INNOVATORS, LTD.
发明人： Naoto KANEKO , Ikuo SHIRATORI , Katsunori HORII , Jou AKITOMI , Iwao WAGA
IPC分类号： G01N33/04 , C12N15/11 , C12N15/115
CPC分类号： G01N33/04 , C12N15/11 , C12N15/115 , C12N2310/127 , C12N2310/151 , C12N2310/16 , C12N2310/3519 , C12Q1/6825 , G01N33/542 , C12Q2521/345 , C12Q2563/107
摘要： The present invention is intended to provide a novel fluorescence sensor for target analysis, a kit for target analysis, and a target analysis method using the same.The fluorescence sensor for target analysis according to the present invention includes a nucleic acid molecule that includes a G-quartet-forming nucleic acid region (D) that forms a G-quartet and a binding nucleic acid region (A) that binds to a target. In the absence of a target, formation of a G-quartet in the G-quartet-forming nucleic acid region (D) is inhibited. In the presence of a target, the target comes into contact with the binding nucleic acid region (A), the G-quartet is formed in the G-quartet-forming nucleic acid region (D) due to the contact, the G-quartet-forming region (D) and porphyrin forms a complex, and the complex generates fluorescence.
摘要翻译：本发明旨在提供一种用于目标分析的新型荧光传感器，用于目标分析的试剂盒和使用该荧光传感器的目标分析方法。根据本发明的用于靶分析的荧光传感器包括核酸分子，其包括形成G-四重态的G-四重峰形成核酸区（D）和与靶标结合的结合核酸区（A）。在不存在目标的情况下，G-四重峰形成核酸区（D）中的G四重奏的形成被抑制。在靶的存在下，目标与结合核酸区域（A）接触，G-四重峰由于接触而在G-四重峰形成核酸区域（D）中形成，G-四重峰形成区域（D）和卟啉形成复合物，并且复合物产生荧光。

6. 发明申请

US20160060630A1 METHOD FOR EVALUATING REDOX ACTIVITY OF NUCLEIC ACID MOLECULE AND NUCLEIC ACID MOLECULE HAVING REDOX ACTIVITY 审中-公开
标题翻译：用于评估具有还原活性的核酸分子和核酸分子的还原活性的方法
公开(公告)号：US20160060630A1
公开(公告)日：2016-03-03
申请号：US14942451
申请日：2015-11-16
申请人： NEC Solution Innovators, Ltd.
发明人： Naoto KANEKO , Katsunori Horii , Jou Akitomi , Shintarou Katou , Iwao Waga
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , C12N15/1034 , C12N15/111 , C12N15/115 , C12N2310/127 , C12N2310/16 , C12N2310/351 , C12N2320/10 , C12N2330/31 , C12Q1/26 , C12Q1/6825 , G01N27/26 , G01N33/581 , C12Q2304/80 , C12Q2525/205 , C12Q2563/113
摘要： The present invention provides a novel technique by which the redox activity of a nucleic acid molecule can be evaluated. An evaluation method of the present invention includes: a detection step of electrochemically detecting a redox reaction to a substrate, the redox reaction being catalyzed by a nucleic acid molecule to be evaluated, using a device that electrochemically detects a redox reaction; and an evaluation step of evaluating redox activity of the nucleic acid molecule from a result of the detection of the redox reaction. As the device, a device in which a base provided with a detection portion is included, the detection portion includes an electrode system, and the nucleic acid molecule to be evaluated is arranged on the base is used. In the present invention, it is preferred that a plurality of kinds of nucleic acid molecule to be evaluated is arranged on the base, and the plurality of kinds of nucleic acid molecules to be evaluated is evaluated by a single device.
摘要翻译：本发明提供可以评价核酸分子的氧化还原活性的新技术。本发明的评价方法包括：使用电化学检测氧化还原反应的装置电化学检测对基底的氧化还原反应的检测步骤，所述氧化还原反应由待评价的核酸分子催化; 以及从检测氧化还原反应的结果来评价核酸分子的氧化还原活性的评价步骤。作为该装置，包括设置有检测部的基板的装置，检测部包括电极系，使用要评价的核酸分子。在本发明中，优选在基材上排列要评价的多种核酸分子，通过单个装置评价要评价的多种核酸分子。

7. 发明申请

US20200248181A1 WHEAT ALLERGEN-BINDING NUCLEIC ACID MOLECULE AND USE THEREOF 审中-公开
公开(公告)号：US20200248181A1
公开(公告)日：2020-08-06
申请号：US15776142
申请日：2015-11-16
申请人： NEC Solution Innovators, Ltd.
发明人： Tomoko SHIRATORI , Naoto KANEKO , Ikuo SHIRATORI , Katsunori HORII , Hirotaka MINAGAWA , Jou AKITOMI , Yoshihito YOSHIDA , Iwao WAGA
IPC分类号： C12N15/115 , C07K14/415 , C12Q1/6883
摘要： The present invention provides a novel nucleic acid molecule that can be used for detection of a wheat allergen. The wheat allergen-binding nucleic acid according to the present invention is characterized in that it binds to a wheat allergen with a dissociation constant of 20 nM or less, and preferably includes a polynucleotide consisting of either of base sequences of SEQ ID NOs: 1 and 2, for example.

8. 发明申请

US20190382765A1 NUCLEOSIDE DERIVATIVE OR SALT THEREOF, POLYNUCLEOTIDE SYNTHESIS REAGENT, METHOD FOR PRODUCING POLYNUCLEOTIDE, POLYNUCLEOTIDE, AND METHOD FOR PRODUCING BINDING NUCLEIC ACID MOLECULE 审中-公开
公开(公告)号：US20190382765A1
公开(公告)日：2019-12-19
申请号：US16464057
申请日：2017-10-12
申请人： NEC Solution Innovators, Ltd. , National University Corporation Gunma University
发明人： Hirotaka MINAGAWA , Katsunori HORII , Jou AKITOMI , Naoto KANEKO , Iwao WAGA , Masayasu KUWAHARA
IPC分类号： C12N15/115 , C12N15/10
摘要： The present invention provides a novel nucleoside derivative or a salt thereof, a polynucleotide synthesis reagent, a method for producing a polynucleotide, a polynucleotide, and a method for producing a binding nucleic acid molecule. The nucleoside derivative or a salt thereof of the present invention is represented by the following chemical formula (1). In the chemical formula (1), Su is an atomic group having a sugar skeleton at a nucleoside residue or an atomic group having a sugar phosphate skeleton at a nucleotide residue, and may or may not have a protecting group, L1 and L2 are each independently a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms, X1 is an imino group (—NR1—), an ether group (—O—), or a thioether group (—S—), and R1 is a hydrogen atom or a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms.

9. 发明申请

US20170356905A1 TARGET ANALYSIS TOOL AND TARGET ANALYSIS METHOD 审中-公开
公开(公告)号：US20170356905A1
公开(公告)日：2017-12-14
申请号：US15545534
申请日：2016-01-22
申请人： NEC Solution Innovators, Ltd.
发明人： Iwao WAGA , Katsunori HORII , Jou AKITOMI , Naoto KANEKO , Yoshihito YOSHIDA
IPC分类号： G01N33/543 , B01L3/00 , G01N33/53
CPC分类号： G01N33/54366 , B01L3/502 , B01L2300/0609 , B01L2300/0672 , B01L2300/0832 , B01L2300/0848 , B01L2300/087 , C12M1/00 , C12Q1/68 , G01N33/5308 , G01N33/54313 , G01N35/02
摘要： A target analysis tool and a target analysis method that allow easily analysis of a target. The first target analysis tool includes: a first chamber; a second chamber; and a third chamber. The first chamber, the second chamber, and the third chamber are disposed continuously in this order. The first chamber contains, as a first reagent, an immobilized first binding substance obtained by immobilizing, on a carrier, a first binding substance that binds to a target. The second chamber contains, as a second reagent, a labeled second binding substance obtained by binding a labeling substance to a second binding substance that binds to the first binding substance. The third chamber is a detection section in which the labeled second binding substance is detected.

10. 发明申请

US20180238867A1 DETECTION DEVICE AND TARGET DETECTION METHOD USING THE SAME 审中-公开
公开(公告)号：US20180238867A1
公开(公告)日：2018-08-23
申请号：US15771962
申请日：2016-10-27
申请人： NEC Solution Innovators, Ltd.
发明人： Katsunori HORII , Naoto KANEKO
IPC分类号： G01N33/543 , G01N27/414
CPC分类号： G01N33/54306 , G01N27/4145 , G01N33/542 , G01N33/54373 , G01N33/54386
摘要： The present invention provides a new detection device and a target detection method using the same. The detection device of the present invention includes a transistor provided with a nucleic acid sensor. The nucleic acid sensor includes a conformation-forming region (D) that forms a predetermined conformation and a binding region (A) that binds to a target. In the absence of the target, the conformation-forming region (D) is inhibited from forming the conformation. In the presence of the target, upon contact of the target to the binding region (A), the conformation-forming region (D) forms the conformation. In a state where the conformation is formed, the number of nucleotide residues that compose the nucleic acid sensor within a range of Debye length of the transistor increases or decreases as compared to a state where formation of the conformation is inhibited.

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