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    • 4. 发明申请
    • Apparatus for and method of purifying nucleic acids by different laser absorption of beads
    • 通过不同激光吸收珠粒来纯化核酸的装置和方法
    • US20060110725A1
    • 2006-05-25
    • US11190169
    • 2005-07-26
    • Jeong-gun LeeYoung-nam KwonMyo-yong LeeShin-i YooYeon-ja ChoYoung-a Kim
    • Jeong-gun LeeYoung-nam KwonMyo-yong LeeShin-i YooYeon-ja ChoYoung-a Kim
    • C12Q1/70C12Q1/68C12N7/02C12N1/08C12M1/34
    • B01L3/502753B01L3/502761B01L7/52B01L2200/0647B01L2200/10B01L2300/0681B01L2300/0867B01L2300/087B01L2400/0487
    • An apparatus for and method of purifying nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples, magnetic beads, and a solid support are introduced; a vibrator attached to the capillary and mixing the samples, magnetic beads, and solid support in the capillary; a laser generator attached to the capillary and irradiating a laser beam onto the capillary; a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall; a waste chamber attached to the capillary and discharging a lysate; an elution buffer chamber attached to the capillary and eluting nucleic acids from the solid support having nucleic acids bound thereto; and a neutralization buffer chamber attached to the capillary and supplying a neutralization buffer for neutralizing the eluted nucleic acid solution. According to the apparatus and method, PCR inhibitors can be removed to increase PCR yield and nucleic acids can be purified using a silicon substrate or silica beads. Thus, the apparatus and method can be applied to LOC fabrication.
    • 提供了一种纯化细胞或病毒核酸的装置和方法。 核酸纯化装置包括:细胞裂解毛细管,其具有样品入口,通过所述样品入口引入样品,磁珠和固体支持物; 连接到毛细管并将样品,磁珠和固体支持物混合在毛细管中的振动器; 激光发生器附接到毛细管并将激光束照射到毛细管上; 附着到毛细管并将磁珠固定到毛细管壁的磁力发生器; 连接到毛细管并排出裂解物的废物室; 洗脱缓冲室连接到毛细管并从具有与其结合的核酸的固体支持物洗脱核酸; 以及中和缓冲室,其连接到毛细管并提供用于中和洗脱的核酸溶液的中和缓冲液。 根据该装置和方法,可以除去PCR抑制剂以增加PCR产量,并且可以使用硅底物或二氧化硅珠纯化核酸。 因此,该装置和方法可以应用于LOC制造。