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1. 发明专利

JP2013090600A Lactic acid producing bacteria 有权
标题翻译：乳酸生产细菌
公开(公告)号：JP2013090600A
公开(公告)日：2013-05-16
申请号：JP2011235485
申请日：2011-10-26
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社 , Hiroshima Univ , 国立大学法人広島大学 , National Institute Of Advanced Industrial Science & Technology , 独立行政法人産業技術総合研究所
发明人： SAKAI SHINSUKE , TAKAOKA KAZUE , NAKASHIMADA YUTAKA , IWASAKI YUKI , YANO SHINICHI , MURAKAMI KATSUJI , KITA AKIHISA , SAWAYAMA SHIGEKI
IPC分类号： C12N15/09 , C12N1/21 , C12P7/56
摘要： PROBLEM TO BE SOLVED: To provide a recombinant bacterium of genus Moorella capable of producing lactic acid.SOLUTION: There are disclosed: a method for producing a recombinant bacterium of genus Moorella capable of producing lactic acid, including a step of incorporating a lactate dehydrogenase (LDH) gene originating from a heterogeneous bacterium into the genome of a bacterium of genus Moorella; a method for incorporating glyceraldehyde-3-phosphate dehydrogenase gene promotor originating from a homogeneous bacterium of genus Moorella into the genome of a bacterium of genus Moorella, wherein, the heterogeneous bacterium belongs to Thermoanaerobacter pseudethanolicus; and a method for producing lactic acid including chemoorganotrophically culturing the recombinant bacterium of genus Moorella produced by the above methods.
摘要翻译：待解决的问题：提供能够产生乳酸的Moorella属的重组细菌。解决方案：公开了一种生产能产生乳酸的Moorella重组细菌的方法，包括将源于异质细菌的乳酸脱氢酶（LDH）基因引入到属的细菌基因组中的步骤莫雷拉将源自Moorella的均质细菌的甘油醛-3-磷酸脱氢酶基因启动子并入Moorella属细菌的基因组中的方法，其中所述异源细菌属于假乙醇假单胞菌; 以及通过上述方法生产乳酸的方法，其包括化学有机营养培养由Moorella属产生的重组细菌。版权所有（C）2013，JPO＆INPIT

2. 发明专利

JP2015023854A モーレラ属細菌の酢酸非生産・乳酸生産株有权
标题翻译：乙醇细菌生产和生产过程中的生产和生产
公开(公告)号：JP2015023854A
公开(公告)日：2015-02-05
申请号：JP2013157077
申请日：2013-07-29
申请人：三井造船株式会社 , Mitsui Eng & Shipbuild Co Ltd , 国立大学法人広島大学 , Hiroshima Univ , 独立行政法人産業技術総合研究所 , National Institute Of Advanced Industrial & Technology
发明人： SHO TOMOHIRO , SAKAI SHINSUKE , SAITO MASAHIRO , NAKASHIMADA YUTAKA , IWASAKI YUKI , HOSHINO TAMOTSU , MURAKAMI KATSUJI , TSUKAHARA KENICHIRO , KITA AKIHISA
IPC分类号： C12N15/09 , C12N1/21 , C12P7/56
摘要：【課題】モーレラ属細菌の酢酸非生産・乳酸生産株を提供する。【解決手段】ホスホトランスアセチラーゼを欠損し、かつ乳酸デヒドロゲナーゼを高発現する、乳酸生産性モーレラ属細菌株。【選択図】なし
摘要翻译：要解决的问题：提供Moorella细菌的乙酸盐非生产和乳酸盐生产菌株。解决方案：产生乳酸的Moorella细菌菌株缺乏磷酸转乙酰酶，并表达高水平的乳酸脱氢酶。

3. 发明专利

JP2010117350A Undifferentiated cell labeling technique using mortalin combining material 有权
标题翻译：使用MORTALIN组合材料的不良细胞标记技术
公开(公告)号：JP2010117350A
公开(公告)日：2010-05-27
申请号：JP2009238973
申请日：2009-10-16
申请人： National Institute Of Advanced Industrial Science & Technology , 独立行政法人産業技術総合研究所
发明人： UEMURA TOSHIKIMI , OYABU YOSHIMI , RENU WADHWA , KAUL SUNIL , MISHIMA HATSU , SAKAI SHINSUKE , YOSHIOKA TOMOKAZU , KAUL ZEENIA
IPC分类号： G01N33/53 , C12Q1/02 , G01N33/543
摘要： PROBLEM TO BE SOLVED: To provide a labeling technique for undifferentiated cell efficiently and stably.
SOLUTION: The present invention relates with a labeling technique of undifferentiated cell which includes the use of labeling complex containing a mortalin combining material with internalized function and a labelled substance combined therewith.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：为了提供对未分化细胞的标记技术的有效和稳定。解决方案：本发明涉及未分化细胞的标记技术，其包括使用含有具有内在功能的mortal组合材料和与其结合的标记物质的复合物。版权所有（C）2010，JPO＆INPIT

4. 发明专利

JP2012249572A Uracil-requiring genus moorella bacterium and transformed gene-transferred genus moorella bacterium 有权
标题翻译：乌拉圭需要的基因转录细菌和转化的转基因马鞭草细菌
公开(公告)号：JP2012249572A
公开(公告)日：2012-12-20
申请号：JP2011124458
申请日：2011-06-02
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社 , Hiroshima Univ , 国立大学法人広島大学 , Gifu Univ , 国立大学法人岐阜大学
发明人： NAKASHIMADA YUTAKA , KITA AKIHISA , SUZUKI TORU , SAKAI SHINSUKE , TAKAOKA KAZUE
IPC分类号： C12N1/21 , C12N15/09
CPC分类号： C12N15/74 , C12N9/88 , C12N2510/00 , C12N2510/02 , C12P7/065 , C12Y401/01023 , Y02E50/17
摘要： PROBLEM TO BE SOLVED: To provide a uracil-requiring genus Moorella bacterium resulting from destroying a gene encoding orotidine-5-phosphate decarboxylase.SOLUTION: The uracil-requiring genus Moorella bacterium is characterized by being MTA-D-pF strain (accession number: NITE P-1057), a uracil-requiring genus Moorella bacterium in which a gene encoding the orotidine-5-phosphate decarboxylase on the chromosome in genus Moorella bacterium is destroyed.
摘要翻译：要解决的问题：提供由破坏编码乳清苷-5-磷酸脱羧酶的基因导致的尿嘧啶需要的摩氏杆菌属细菌。解决方案：需要尿嘧啶需要的摩氏杆菌属细菌的特征在于MTA-D-pF菌株（登录号：NITE P-1057），其中编码乳清苷-5-磷酸的基因的尿嘧啶需要的Moorella细菌毛霉菌属染色体上的脱羧酶被破坏。版权所有（C）2013，JPO＆INPIT

5. 发明专利

JP2010017131A Bacteria of genus moorella and primer 审中-公开
标题翻译：。。。。。。。。。。。
公开(公告)号：JP2010017131A
公开(公告)日：2010-01-28
申请号：JP2008180657
申请日：2008-07-10
申请人： Hiroshima Univ , Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社 , 国立大学法人広島大学
发明人： NISHIO NAOMICHI , NAKASHIMADA YUTAKA , SAKAI SHINSUKE
IPC分类号： C12N15/09 , C12N1/20 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide bacteria of the genus Moorella which are available for wide uses as a host cell, and a primer which is available upon creation of bacteria of the genus Moorella which are available for wide uses as a host cell.
SOLUTION: Disclosed are bacteria of the genus Moorella having features that genes on chromosomes are deleted or destroyed by homologous recombination to exhibit auxotrophy, and a specific primer which amplifies a region adjacent to a gene encoding orotate phosphoribosyltransferase.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供可广泛用作宿主细胞的Moorella属的细菌，以及可用于广泛用作宿主细胞的Moorella属细菌产生的引物。解决方案：公开了Moorella属的细菌，其特征在于染色体上的基因通过同源重组而缺失或破坏以显示营养缺陷型，以及扩增与编码乳酸脱氢磷酸核糖基转移酶的基因相邻的区域的特异性引物。版权所有（C）2010，JPO＆INPIT

6. 发明专利

JP2012249573A Primer set and method for homologous recombination 有权
标题翻译：用于同种异体重建的前置集和方法
公开(公告)号：JP2012249573A
公开(公告)日：2012-12-20
申请号：JP2011124459
申请日：2011-06-02
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社 , Hiroshima Univ , 国立大学法人広島大学 , Gifu Univ , 国立大学法人岐阜大学
发明人： NAKASHIMADA YUTAKA , KITA AKIHISA , SUZUKI TORU , SAKAI SHINSUKE , TAKAOKA KAZUE
IPC分类号： C12N15/09 , C12N1/21
CPC分类号： C12N15/902 , C12N9/88 , C12N15/74 , C12P7/065 , C12Y401/01023 , Y02E50/17
摘要： PROBLEM TO BE SOLVED: To provide a primer set which is used for transformation for imparting uracil requirement in which a gene encoding orotidine-5-phosphate decarboxylase is deleted or destroyed, in genus Moorella bacterium.SOLUTION: The primer set used when producing an uracil-requiring strain whose gene encoding orotidine-5-phosphate decarboxylase is deleted or destroyed by homologous recombination in genus Moorella bacterium is characterized by being represented by a specific sequence for amplifying an upstream domain adjacent to the gene encoding the orotidine-5-phosphate decarboxylase.
摘要翻译：待解决的问题：提供一种引物组，其用于赋予尿嘧啶要求的转化，其中编码乳清苷-5-磷酸脱羧酶的基因在Moorella细菌中被缺失或破坏。解决方案：在制备尿嘧啶需要菌株时使用的引物组，其编码乳清苷-5-磷酸脱羧酶的基因在Moorella细菌中被同源重组缺失或破坏，其特征在于用于扩增上游结构域的特异性序列与编码乳清苷-5-磷酸脱羧酶的基因相邻。版权所有（C）2013，JPO＆INPIT

7. 发明专利

JP2011254827A Yeast, and method for breeding the same 有权
标题翻译： YEAST及其育种方法
公开(公告)号：JP2011254827A
公开(公告)日：2011-12-22
申请号：JP2011161849
申请日：2011-07-25
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社
发明人： SAKAI SHINSUKE , IWAI TOMOKO , TAKAOKA KAZUE
IPC分类号： C12N1/19 , C12N15/09
CPC分类号： C12P7/06 , C07K14/39 , C12C2200/05 , C12G2200/11 , Y02E50/17
摘要： PROBLEM TO BE SOLVED: To provide a method for breeding an yeast imparted or enhanced with its aggregating property by utilizing a polypeptide having activity to impart the aggregating property to the yeast and a genetic DNA encoding an amino acid sequence having the activity to impart the aggregating property to the yeast.SOLUTION: This yeast is characterized by imparting or enhancing its aggregating property by the genetic modification including an amino acid sequence having ≥4-time repetitions of a sequence into an FLO protein in an amino acid sequence consisting of a specific sequence, and the method for producing the yeast includes imparting or enhancing the aggregating property by the genetic modification including the amino acid sequence having ≥4-time repetitions of the sequence into the FLO protein in the amino acid sequence consisting of the specific sequence.
摘要翻译：待解决的问题：提供一种通过利用具有赋予酵母的聚集性质的活性的多肽和编码具有活性的氨基酸序列的遗传DNA来提供赋予或增强的具有聚集性的酵母的方法，赋予酵母的聚集性能。解决方案：该酵母的特征在于通过遗传修饰赋予或增强其聚集性质，包括在具有特定序列的氨基酸序列中具有≥4次重复序列的氨基酸序列到FLO蛋白中，以及生产酵母的方法包括通过遗传修饰赋予或增强包含具有≥4次重复序列的氨基酸序列的聚集性质到由特定序列组成的氨基酸序列中的FLO蛋白质中。版权所有（C）2012，JPO＆INPIT

8. 发明专利

JP2014183780A Ethanol production system 审中-公开
标题翻译：乙醇生产系统
公开(公告)号：JP2014183780A
公开(公告)日：2014-10-02
申请号：JP2013060981
申请日：2013-03-22
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社
发明人： SHO TOMOHIRO , SAITO MASAHIRO , SAKAI SHINSUKE , TAKAOKA KAZUE , KURAKAKE TADASHI
IPC分类号： C12M1/00 , C12P7/08
摘要： PROBLEM TO BE SOLVED: To provide an ethanol production system which can easily maintain an alcohol in fermentation liquor in a low concentration, and can reduce energy consumption at the time of purifying the alcohol contained in the fermentation liquor in a low concentration.SOLUTION: An ethanol production system includes: a fermentation tank 11 including a fermentation tank body 11a storing fermentation liquor containing gas-assimilating bacteria, and a raw material gas introducing pipe 12 having a raw material gas introducing port 12a which is connected to the fermentation tank body 11a and introduces the raw material gas into the fermentation liquor in the fermentation tank body 11a, the fermentation tank 11 converting the introduced raw material gas into ethanol by a metabolic reaction of the gas-assimilating bacteria to produce ethanol in the fermentation liquor; and a dealcoholization film device 2 having a dealcoholization film which selectively separates ethanol into the permeation side by supplying the fermentation liquor containing ethanol generated by the fermentation tank 11.
摘要翻译：要解决的问题：提供一种乙醇生产系统，其可以容易地在低浓度的发酵液中维持醇，并且可以降低在低浓度下纯化发酵液中所含的醇时的能量消耗。解决方案：乙醇生产系统包括：发酵罐11，其包含存储含有气体同化细菌的发酵液的发酵罐体11a和具有连接到发酵罐体11a的原料气体导入口12a的原料气体导入管12，将原料气体引入发酵槽体11a的发酵液中，发酵罐11通过气体同化细菌的代谢反应将引入的原料气体转化为乙醇，在发酵液中生成乙醇; 和具有脱醇膜的脱醇膜装置2，其通过供给由发酵罐11产生的含有乙醇的发酵液选择性地将乙醇分离成渗透侧。

9. 发明专利

JP2012205530A Ethanol production apparatus and method 审中-公开
标题翻译：乙醇生产设备和方法
公开(公告)号：JP2012205530A
公开(公告)日：2012-10-25
申请号：JP2011072975
申请日：2011-03-29
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社
发明人： MIYAJI TAKESHI , SAKAI SHINSUKE , SUGIMOTO TOMIO , TAKAOKA KAZUE
IPC分类号： C12M1/04 , C12P7/06
CPC分类号： C12M21/12 , C12M29/26 , C12M43/00 , C12M43/02 , C12M47/10 , Y02E50/17
摘要： PROBLEM TO BE SOLVED: To provide an ethanol production apparatus and method for efficiently producing ethanol at low cost by biochemistry conversion and catalytic reaction from a gas containing carbon monoxide and hydrogen.SOLUTION: The ethanol production apparatus includes: a gas separating unit that separates the raw material gas containing carbon monoxide and hydrogen into a hydrogen gas and a gas containing the carbon monoxide; a first microorganism treatment tank to which the gas containing the carbon monoxide separated in the gas separation unit is sent to generate acetic acid from the gas containing the carbon monoxide by biological conversion; and a catalytic reaction unit where at least the hydrogen gas separated in the gas separation unit and at least the acetic acid generated in the first microorganism treatment tank are made to react in the presence of a catalyst to generate ethanol.
摘要翻译：待解决的问题：提供一种通过生物化学转化和催化反应从含有一氧化碳和氢气的气体中低成本生产乙醇的乙醇生产装置和方法。解决方案：乙醇生产设备包括：气体分离单元，其将含有一氧化碳和氢气的原料气体分离成氢气和含有一氧化碳的气体; 在气体分离单元中分离出含有一氧化碳的气体的第一微生物处理槽通过生物转化从含有一氧化碳的气体中产生乙酸; 以及催化反应单元，其中至少在气体分离单元中分离的氢气和至少在第一微生物处理槽中产生的乙酸在催化剂存在下反应生成乙醇。版权所有（C）2013，JPO＆INPIT

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