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1. 发明专利

JP2012249573A Primer set and method for homologous recombination 有权
标题翻译：用于同种异体重建的前置集和方法
公开(公告)号：JP2012249573A
公开(公告)日：2012-12-20
申请号：JP2011124459
申请日：2011-06-02
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社 , Hiroshima Univ , 国立大学法人広島大学 , Gifu Univ , 国立大学法人岐阜大学
发明人： NAKASHIMADA YUTAKA , KITA AKIHISA , SUZUKI TORU , SAKAI SHINSUKE , TAKAOKA KAZUE
IPC分类号： C12N15/09 , C12N1/21
CPC分类号： C12N15/902 , C12N9/88 , C12N15/74 , C12P7/065 , C12Y401/01023 , Y02E50/17
摘要： PROBLEM TO BE SOLVED: To provide a primer set which is used for transformation for imparting uracil requirement in which a gene encoding orotidine-5-phosphate decarboxylase is deleted or destroyed, in genus Moorella bacterium.SOLUTION: The primer set used when producing an uracil-requiring strain whose gene encoding orotidine-5-phosphate decarboxylase is deleted or destroyed by homologous recombination in genus Moorella bacterium is characterized by being represented by a specific sequence for amplifying an upstream domain adjacent to the gene encoding the orotidine-5-phosphate decarboxylase.
摘要翻译：待解决的问题：提供一种引物组，其用于赋予尿嘧啶要求的转化，其中编码乳清苷-5-磷酸脱羧酶的基因在Moorella细菌中被缺失或破坏。解决方案：在制备尿嘧啶需要菌株时使用的引物组，其编码乳清苷-5-磷酸脱羧酶的基因在Moorella细菌中被同源重组缺失或破坏，其特征在于用于扩增上游结构域的特异性序列与编码乳清苷-5-磷酸脱羧酶的基因相邻。版权所有（C）2013，JPO＆INPIT

2. 发明专利

JP2012249572A Uracil-requiring genus moorella bacterium and transformed gene-transferred genus moorella bacterium 有权
标题翻译：乌拉圭需要的基因转录细菌和转化的转基因马鞭草细菌
公开(公告)号：JP2012249572A
公开(公告)日：2012-12-20
申请号：JP2011124458
申请日：2011-06-02
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社 , Hiroshima Univ , 国立大学法人広島大学 , Gifu Univ , 国立大学法人岐阜大学
发明人： NAKASHIMADA YUTAKA , KITA AKIHISA , SUZUKI TORU , SAKAI SHINSUKE , TAKAOKA KAZUE
IPC分类号： C12N1/21 , C12N15/09
CPC分类号： C12N15/74 , C12N9/88 , C12N2510/00 , C12N2510/02 , C12P7/065 , C12Y401/01023 , Y02E50/17
摘要： PROBLEM TO BE SOLVED: To provide a uracil-requiring genus Moorella bacterium resulting from destroying a gene encoding orotidine-5-phosphate decarboxylase.SOLUTION: The uracil-requiring genus Moorella bacterium is characterized by being MTA-D-pF strain (accession number: NITE P-1057), a uracil-requiring genus Moorella bacterium in which a gene encoding the orotidine-5-phosphate decarboxylase on the chromosome in genus Moorella bacterium is destroyed.
摘要翻译：要解决的问题：提供由破坏编码乳清苷-5-磷酸脱羧酶的基因导致的尿嘧啶需要的摩氏杆菌属细菌。解决方案：需要尿嘧啶需要的摩氏杆菌属细菌的特征在于MTA-D-pF菌株（登录号：NITE P-1057），其中编码乳清苷-5-磷酸的基因的尿嘧啶需要的Moorella细菌毛霉菌属染色体上的脱羧酶被破坏。版权所有（C）2013，JPO＆INPIT

3. 发明专利

JP2013090600A Lactic acid producing bacteria 有权
标题翻译：乳酸生产细菌
公开(公告)号：JP2013090600A
公开(公告)日：2013-05-16
申请号：JP2011235485
申请日：2011-10-26
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社 , Hiroshima Univ , 国立大学法人広島大学 , National Institute Of Advanced Industrial Science & Technology , 独立行政法人産業技術総合研究所
发明人： SAKAI SHINSUKE , TAKAOKA KAZUE , NAKASHIMADA YUTAKA , IWASAKI YUKI , YANO SHINICHI , MURAKAMI KATSUJI , KITA AKIHISA , SAWAYAMA SHIGEKI
IPC分类号： C12N15/09 , C12N1/21 , C12P7/56
摘要： PROBLEM TO BE SOLVED: To provide a recombinant bacterium of genus Moorella capable of producing lactic acid.SOLUTION: There are disclosed: a method for producing a recombinant bacterium of genus Moorella capable of producing lactic acid, including a step of incorporating a lactate dehydrogenase (LDH) gene originating from a heterogeneous bacterium into the genome of a bacterium of genus Moorella; a method for incorporating glyceraldehyde-3-phosphate dehydrogenase gene promotor originating from a homogeneous bacterium of genus Moorella into the genome of a bacterium of genus Moorella, wherein, the heterogeneous bacterium belongs to Thermoanaerobacter pseudethanolicus; and a method for producing lactic acid including chemoorganotrophically culturing the recombinant bacterium of genus Moorella produced by the above methods.
摘要翻译：待解决的问题：提供能够产生乳酸的Moorella属的重组细菌。解决方案：公开了一种生产能产生乳酸的Moorella重组细菌的方法，包括将源于异质细菌的乳酸脱氢酶（LDH）基因引入到属的细菌基因组中的步骤莫雷拉将源自Moorella的均质细菌的甘油醛-3-磷酸脱氢酶基因启动子并入Moorella属细菌的基因组中的方法，其中所述异源细菌属于假乙醇假单胞菌; 以及通过上述方法生产乳酸的方法，其包括化学有机营养培养由Moorella属产生的重组细菌。版权所有（C）2013，JPO＆INPIT

4. 发明专利

JP2009225756A Method for continuously producing alcohol 有权
标题翻译：连续生产醇的方法
公开(公告)号：JP2009225756A
公开(公告)日：2009-10-08
申请号：JP2008077765
申请日：2008-03-25
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社
发明人： TOYAMA MASAYUKI , TAKAOKA KAZUE
IPC分类号： C12P7/06 , C12R1/865
CPC分类号： Y02E50/17
摘要： PROBLEM TO BE SOLVED: To provide a method for continuously producing a high concentration alcohol capable of making full use of the ability to produce an alcohol of yeast having a property of coagulation and sedimentation by preventing the outflow of yeast cells and improving the contact efficiency between alcohol raw materials and the yeast.
SOLUTION: The method for continuously producing an alcohol by which an alcohol is produced by forcibly causing a liquid flow to occur in a lower side of a fermentation tank when alcohol fermentation is performed by supplying alcohol raw materials to the fermentation tank in the presence of the yeast having the property of coagulation and sedimentation, characterized by controlling a liquid flow in the lower side of the fermentation tank such that yeast cell concentrations d1, d2 and d3 are measured at different positions in the vertical direction of the alcohol fermentation liquid in the fermentation tank and a ratio of d1 to d2 (d2/d1) becomes 1.7 or more and a ratio of d1 to d3 (d3/d1) becomes 7.6 or less.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：要解决的问题：提供一种连续制备能够充分利用通过防止酵母细胞流出而产生具有凝结和沉淀性质的酵母醇的能力的高浓度醇的方法，酒精原料与酵母的接触效率。解决方案：当通过向发酵罐中提供酒精原料进行酒精发酵时，连续生产醇的方法是通过强制引发发酵罐下侧发生液体流而产生醇的方法存在具有凝结和沉淀性质的酵母，其特征在于控制发酵罐下侧的液体流动，使得在酒精发酵液体的垂直方向上的不同位置测量酵母细胞浓度d1，d2和d3 在发酵槽中，d1〜d2（d2 / d1）的比例为1.7以上，d1〜d3（d3 / d1）的比为7.6以下。版权所有（C）2010，JPO＆INPIT

5. 发明专利

JP2009072144A Method for saccharifying celluloses 有权
标题翻译：用于分离细胞的方法
公开(公告)号：JP2009072144A
公开(公告)日：2009-04-09
申请号：JP2007245455
申请日：2007-09-21
申请人： Mitsui Eng & Shipbuild Co Ltd , Univ Nagoya , 三井造船株式会社 , 国立大学法人名古屋大学
发明人： KOBAYASHI SHINSUKE , TAKAOKA KAZUE , TOYAMA MASAYUKI , KATAGIRI MANABU
IPC分类号： C12P19/14
摘要： PROBLEM TO BE SOLVED: To provide a method capable of efficiently promoting hydrolysis of celluloses by an enzyme while keeping lignin in a system without carrying out oxidative decomposition of lignin.
SOLUTION: The method for saccharifying the celluloses comprises saccharifying celluloses contained in biomass such as a non-edible part of farm products and ligneous unused material or waste material by using a hydrolase. The method for saccharifying the celluloses is characterized in that a substance having high affinity for lignin is previously added to celluloses and then, hydrolysis reaction of the celluloses is started by the hydrolase.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供一种能够有效地促进酶的纤维素水解，同时将木质素保持在体系中而不进行木质素的氧化分解的方法。糖化纤维素的方法包括通过使用水解酶来糖化生物质中包含的纤维素，例如农产品的不可食用部分和木质未使用的材料或废料。糖化纤维素的方法的特征在于，预先将对木质素具有高亲和力的物质加入到纤维素中，然后通过水解酶开始纤维素的水解反应。版权所有（C）2009，JPO＆INPIT

6. 发明专利

JP2008237055A Method for producing rice saccharified liquid 有权
标题翻译：生产米汁液体的方法
公开(公告)号：JP2008237055A
公开(公告)日：2008-10-09
申请号：JP2007079573
申请日：2007-03-26
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社
发明人： TOYAMA MASAYUKI , TAKAOKA KAZUE
IPC分类号： C12P19/02 , A23L7/104
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a rice saccharified liquid for remarkably improving the saccharification efficiency of rice, by carrying out a pretreatment with an acid and a high-temperature and a high-pressure when the rice saccharified liquid is produced with a normal enzyme. SOLUTION: The method for producing the rice saccharified liquid is carried out by introducing rice flour and water in 1:(1.5-2.5) mixing ratio (weight ratio) into a vessel, mixing and stirring both, then adding hydrochloric acid to the mixture liquid in the vessel, regulating the pH to 1.5-2.5, subsequently conducting a pretreatment of heating the mixture liquid, after the acid treatment in a pressurized vessel at a temperature within the range of 100-130°C for 15-25 min, and then carrying out saccharification with the enzyme. COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：为了提供一种用于制造米糖化液的方法，用于显着提高米的糖化效率，通过在水分糖化液体中进行酸和高温高压的预处理用正常酶产生。解决方案：通过将米粉和水以1：（1.5-2.5）的混合比（重量比）引入容器中进行，混合搅拌，然后加入盐酸至在容器中的混合液体中，将pH调节至1.5-2.5，随后在加压容器中在100-130℃的温度下酸处理15-25分钟后进行加热混合液体的预处理，然后用酶进行糖化。版权所有（C）2009，JPO＆INPIT

7. 发明专利

JP2006280262A Method for measuring contaminant-degrading ability of microorganism 有权
标题翻译：测定微生物污染物降解能力的方法
公开(公告)号：JP2006280262A
公开(公告)日：2006-10-19
申请号：JP2005103710
申请日：2005-03-31
申请人： Mitsui Eng & Shipbuild Co Ltd , Toyohashi Univ Of Technology , 三井造船株式会社 , 国立大学法人豊橋技術科学大学
发明人： HIRAISHI AKIRA , TOYAMA MASAYUKI , NAKATANI TATSUO , TAKAOKA KAZUE , IWAI TOMOKO
IPC分类号： C12Q1/02 , B09C1/10
摘要： PROBLEM TO BE SOLVED: To provide a method for quickly monitoring the cleanup status in the process for the cleanup of an object contaminated with organic matter utilizing the microbial reaction in the object, without conducting a high-sensitivity contaminant analysis needing long time and high cost.
SOLUTION: The method comprises the pretreatment step of removing microorganism-undigested organic matter from a microorganism-containing contaminated object, the step of constructing, from the pretreated contaminated object, a contaminant culture system provided with only organic matter as contamination source as a respiratory substrate and a universal culture system provided with living organic matter free of the above organic matter, and the step of measuring the respiratory activity of the microorganisms in each of the above culture systems. By comparing the respiratory activity of the contaminant culture system with that of the universal culture system, the ability of the microorganisms in the contaminated object to degrade the contamination source is measured.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：要解决的问题：提供一种用于在物体中利用微生物反应清洁被有机物污染的物体的过程中快速监测清洁状态的方法，而不需要长时间的高灵敏度污染物分析成本高。解决方案：该方法包括从含微生物的污染物质中除去微生物未消化的有机物质的预处理步骤，从预处理的污染物质构建仅提供有机物质的污染物培养系统作为污染源的步骤呼吸基质和具有不含上述有机物的活体有机物的通用培养体系，以及测定上述培养体系中的微生物的呼吸活性的步骤。通过比较污染物培养系统的呼吸活性与通用培养系统的呼吸活性，测量被污染物质中的微生物降解污染源的能力。版权所有（C）2007，JPO＆INPIT

8. 发明专利

JP2005262107A Method for cleaning matter contaminated with organochlorine compound 审中-公开
标题翻译：用有机氯化合物污染物质的清洗方法
公开(公告)号：JP2005262107A
公开(公告)日：2005-09-29
申请号：JP2004079373
申请日：2004-03-19
申请人： Internat Center For Environmental Technol Transafer , Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社 , 財団法人国際環境技術移転研究センター
发明人： NIHEI HIROYUKI , NAKATANI TATSUO , TAKAOKA KAZUE
IPC分类号： A62D3/02 , A62D3/30 , A62D101/22 , B09B3/00 , B09C1/02 , B09C1/08 , C07D319/24 , A62D3/00
CPC分类号： Y02W30/78
摘要： PROBLEM TO BE SOLVED: To provide a technique for efficiently removing an organochlorine compound from the matter to be treated such as soil at a high biological utilization rate when the matter contaminated with the organochlorine compound such as dioxins is cleaned by the action of a microbe.
SOLUTION: This method for cleaning the matter contaminated with the organochlorine compound comprises the steps of: adding a sustained-release base material containing oil, fat and/or a surfactant to the matter which is to be treated and is contaminated with the organochlorine compound; removing the organochlorine compound by releasing the oil, fat and/or surfactant from the sustained-release base material; and decomposing the removed organochlorine compound by the action of the microbe.
COPYRIGHT: (C)2005,JPO&NCIPI
摘要翻译：待解决的问题：提供一种以有机氯化合物如二恶英污染的物质以高生物利用率有效地从被处理物质如土壤中有效除去有机氯化合物的技术，微生物解决方案：用于清洁被有机氯化合物污染的物质的方法包括以下步骤：向待处理的物质中加入含有油，脂肪和/或表面活性剂的缓释基质，并被污染有机氯化合物; 通过从持续释放基材中释放油，脂肪和/或表面活性剂来除去有机氯化合物; 并通过微生物的作用分解去除的有机氯化合物。版权所有（C）2005，JPO＆NCIPI

9. 发明专利

JP2004223392A Modifier and modifying method 审中-公开
公开(公告)号：JP2004223392A
公开(公告)日：2004-08-12
申请号：JP2003013409
申请日：2003-01-22
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社
发明人： TAKAOKA KAZUE
IPC分类号： C02F1/00 , C02F11/00
摘要： PROBLEM TO BE SOLVED: To obtain a modifier for sewage or sludge which suppresses the occurrence of an offensive smell component without exerting an adverse effect on sewage or sludge, and to provide a modifying method.
SOLUTION: It is considered that secondary synthetic metabolism actively acts on an actinomyces living in sewage or sludge by allowing copper ions to be present in the sewage or sludge to accelerate the production of an antibiotic, the synthesis of melanin, or the like, and sulfur is taken in the synthesized antibiotic or melanin to be fixed as a stable organic substance. Since sulfur is not taken in the offensive smell component such as hydrogen sulfide, methyl mercapton, it is estimated that the formation of the offensive smell component can be suppressed. That is, by allowing copper ions to be present in sewage or sludge, the sewage or sludge can be modified so as not to produce the offensive smell component.
COPYRIGHT: (C)2004,JPO&NCIPI

10. 发明专利

JP2009225755A Method for continuously producing alcohol 审中-公开
标题翻译：连续生产醇的方法
公开(公告)号：JP2009225755A
公开(公告)日：2009-10-08
申请号：JP2008077763
申请日：2008-03-25
申请人： Mitsui Eng & Shipbuild Co Ltd , 三井造船株式会社
发明人： TOYAMA MASAYUKI , TAKAOKA KAZUE
IPC分类号： C12P7/06 , C12G3/02
CPC分类号： Y02E50/17
摘要： PROBLEM TO BE SOLVED: To provide a method for continuously producing an alcohol capable of making full use of the ability to produce an alcohol of yeast having a property of coagulation and sedimentation by preventing the outflow of yeast cells and improving the contact efficiency between alcohol raw materials and the yeast in continuously producing the alcohol.
SOLUTION: The method for continuously producing an alcohol by which an alcohol is produced by forcibly causing a liquid flow to occur in a lower side of a fermentation tank when alcohol fermentation is performed by supplying alcohol raw materials to the fermentation tank in the presence of the yeast having the property of coagulation and sedimentation, characterized by performing alcohol fermentation in a state where an interface of the yeast is formed.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供一种连续制备醇的方法，其能够充分利用通过防止酵母细胞流出并提高接触效率来产生具有凝结和沉淀性质的酵母醇的能力在酒精原料和酵母之间连续生产酒精。解决方案：当通过向发酵罐中提供酒精原料进行酒精发酵时，连续生产醇的方法是通过强制引发发酵罐下侧发生液体流而产生醇的方法存在具有凝结和沉淀性质的酵母，其特征在于在形成酵母界面的状态下进行酒精发酵。版权所有（C）2010，JPO＆INPIT

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