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1. 发明申请

US20100068713A1 PROBE FOR DETECTING MUTATION IN JAK2 GENE AND USE THEREOF 审中-公开
标题翻译：用于检测JAK2基因中的突变的探针及其用途
公开(公告)号：US20100068713A1
公开(公告)日：2010-03-18
申请号：US12513642
申请日：2008-07-11
申请人： Mitsuharu Hirai , Satoshi Majima , Taira Maekawa , Shinya Kimura
发明人： Mitsuharu Hirai , Satoshi Majima , Taira Maekawa , Shinya Kimura
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/04
CPC分类号： C12Q1/6883 , C12Q2600/156 , Y10T436/143333
摘要： A probe for detecting a mutation in the JAK2 gene is provided that can detect a target sequence containing a mutation even when the target sequence containing the mutation and a non-target sequence containing no mutation coexist, which are different only in a single base from each other. The probe to be used is an oligonucleotide that is at least one oligonucleotide having a sequence identical to that of a region extending from a cytosine base (C) at position 84 to be considered as the first base to any one of the 17th to 22nd bases in the direction toward the 5′ end in exon 12 of the JAK2 gene consisting of the base sequence of SEQ ID NO: 1, with the cytosine base (C) being the 3′ end. Even in the case of a sample containing both the JAK2 genes with a mutation that has occurred and without a mutation that has occurred, the use of such a probe in, for example, Tm analysis allows the former mutation to be detected. Preferably, the probe is labeled with a fluorescent dye.
摘要翻译：提供了用于检测JAK2基因突变的探针，即使当含有突变的靶序列和不含突变的非靶序列共存时也可以检测到含有突变的靶序列，其仅在单个碱基中不同其他。要使用的探针是至少一种寡核苷酸，其具有与从第84位的胞嘧啶碱基（C）延伸的区域的序列相同的寡核苷酸，被认为是第17位至第22位碱基的第一碱基在由SEQ ID NO：1的碱基序列组成的JAK2基因的外显子12的5'端的方向上，胞嘧啶碱基（C）为3'末端。即使在含有具有发生突变且没有发生突变的JAK2基因的样品的情况下，在例如Tm分析中使用这种探针也可以检测到前述突变。优选地，探针用荧光染料标记。

2. 发明申请

US20090176231A1 PROBE FOR DETECTING ABL GENE MUTATION AND USES THEREOF 有权
标题翻译：检测ABL基因突变的探针及其用途
公开(公告)号：US20090176231A1
公开(公告)日：2009-07-09
申请号：US12293916
申请日：2008-02-19
申请人： Mitsuharu Hirai , Satoshi Majima , Taira Maekawa , Shinya Kimura
发明人： Mitsuharu Hirai , Satoshi Majima , Taira Maekawa , Shinya Kimura
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6886 , C12Q2600/172
摘要： Detection probes are provided that are capable of detecting a sequence to be detected containing a mutation even when a sequence not to be detected containing no mutation coexists with the sequence to be detected containing a mutation, which are different only in a single base from each other. At least one oligonucleotide selected from the group consisting of SEQ ID NOs: 2˜16 is used as a probe. Even in a sample containing an abl gene in which a mutation has occurred and an abl gene in which no mutation has occurred, the use of such probes in, for example, Tm analysis allows the mutation to be detected.
摘要翻译：提供检测探针，即使在不含检测的序列的情况下，也可以检测到含有突变的检测序列，所述序列与含有突变的检测序列共存，所述突变仅在单个碱基中不同。使用选自SEQ ID NO：2〜16的至少一种寡核苷酸作为探针。即使在含有发生突变的abl基因的样品和没有发生突变的abl基因的情况下，在例如Tm分析中使用这种探针也可以检测突变。

3. 发明授权

US09012619B2 Probe for detecting ABL gene mutation and uses thereof 有权
标题翻译：探测ABL基因突变及其用途
公开(公告)号：US09012619B2
公开(公告)日：2015-04-21
申请号：US12293916
申请日：2008-02-19
申请人： Mitsuharu Hirai , Satoshi Majima , Taira Maekawa , Shinya Kimura
发明人： Mitsuharu Hirai , Satoshi Majima , Taira Maekawa , Shinya Kimura
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6886 , C12Q2600/172
摘要： Detection probes are provided that are capable of detecting a sequence to be detected containing a mutation even when a sequence not to be detected containing no mutation coexists with the sequence to be detected containing a mutation, which are different only in a single base from each other. At least one oligonucleotide selected from the group consisting of SEQ ID NOs: 2˜16 is used as a probe. Even in a sample containing an abl gene in which a mutation has occurred and an abl gene in which no mutation has occurred, the use of such probes in, for example, Tm analysis allows the mutation to be detected.
摘要翻译：提供检测探针，即使在不含检测的序列的情况下，也可以检测到含有突变的检测序列，所述序列与含有突变的检测序列共存，所述突变仅在单个碱基中不同。使用选自SEQ ID NO：2〜16的至少一种寡核苷酸作为探针。即使在含有发生突变的abl基因的样品和没有发生突变的abl基因的情况下，也可以使用例如Tm分析中的这种探针来检测突变。

4. 发明申请

US20100216123A1 METHOD OF DETECTING MUTATION AND KIT USED IN THE SAME 审中-公开
标题翻译：检测其中的突变和套件的方法
公开(公告)号：US20100216123A1
公开(公告)日：2010-08-26
申请号：US12376534
申请日：2007-07-27
申请人： Mitsuharu Hirai , Satoshi Majima , Taira Maekawa , Shinya Kimura
发明人： Mitsuharu Hirai , Satoshi Majima , Taira Maekawa , Shinya Kimura
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6827 , C12Q2527/107 , C12Q2537/143
摘要： A method of detecting a mutation is provided that uses Tm analysis and is excellent in detection sensitivity. A detection probe consisting of a polynucleotide complementary to a sequence to be detected containing a detection site that has been mutated and an inhibitory polynucleotide complementary to a sequence not to be detected containing the detection site that is unmutated are added to a sample containing a DNA to be detected in which the detection site has been mutated and a DNA not to be detected in which the detection site is unmutated, so that the detection probe is hybridized with the DNA. Then while the hybridization product between the DNA and the detection probe is heated, a signal variation associated with an increase in temperature is measured, then the signal variation is analyzed, and thereby a Tm value is determined, based on which the presence of the mutation is determined.
摘要翻译：提供检测突变的方法，其使用Tm分析，检测灵敏度优异。将含有与待检测的序列互补的多核苷酸的检测探针与含有未突变的检测部位的未检测部位互补的抑制性多核苷酸添加到含有检测部位的检测位点上，检测其中已检测位点已被突变，并且检测不被检测的DNA未突变，使得检测探针与DNA杂交。然后，当加热DNA和检测探针之间的杂交产物时，测量与温度升高相关联的信号变化，然后分析信号变化，从而确定Tm值，基于该值，存在突变决心，决意，决定。

5. 发明申请

US20090208956A1 PRIMER SET FOR AMPLIFYING CYP2C9 GENE, REAGENT FOR AMPLIFYING CYP2C9 GENE CONTAINING THE SAME, AND THE USES THEREOF 审中-公开
标题翻译：用于放大CYP2C9基因的准备组，用于放大含有CYP2C9基因的CYP2C9基因的试剂及其用途
公开(公告)号：US20090208956A1
公开(公告)日：2009-08-20
申请号：US12307516
申请日：2007-11-30
申请人： Mitsuharu Hirai , Satoshi Majima
发明人： Mitsuharu Hirai , Satoshi Majima
IPC分类号： C12Q1/68 , C07H21/00 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q1/6883 , C12Q2600/156
摘要： A primer set for amplifying a region including a site to be detected of CT2C9*3 in the C-YT2C9 gene by a gene amplification method is provided, wherein the primer set can amplify the region specifically. A pair of primer set is used including a forward primer consisting of the base sequence of SEQ ID NO: 4 as well as a reverse primer consisting of the base sequence of SEQ ID NO: 17. The use of this primer set makes it possible to specifically and efficiently amplify a target region including the site where a polymorphism, CYP2C9*3, of the CYP2C9 gene is generated.
摘要翻译：提供了通过基因扩增方法扩增C-YT2C9基因中包含CT2C9 * 3的待检测部位的区域的引物组，其中引物组可以特异性地扩增该区域。使用一对引物组，包括由SEQ ID NO：4的碱基序列组成的正向引物以及由SEQ ID NO：17的碱基序列组成的反向引物。使用该引物组使得可以特异性且有效地扩增包含生成CYP2C9基因的多态性CYP2C9 * 3的位点的靶区域。

6. 发明授权

US09175272B2 Probes for detection of UGT1A1 gene, reagent containing the same, and the uses thereof 有权
标题翻译：用于检测UGT1A1基因的检测器，含有该UGT1A1基因的试剂及其用途
公开(公告)号：US09175272B2
公开(公告)日：2015-11-03
申请号：US13078285
申请日：2011-04-01
申请人： Mitsuharu Hirai , Satoshi Majima
发明人： Mitsuharu Hirai , Satoshi Majima
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/10
CPC分类号： C12N9/1051 , C12Q1/6886 , C12Q2600/156 , C12Q2600/16
摘要： Primer sets for amplifying target regions containing sites to be detected in the UGT1A1 gene by a gene amplification method are provided, wherein the primer sets can amplify the regions specifically Three pairs of primer sets are used including forward primers consisting of the base sequences of SEQ ID NOs: 4 or 81, 21, and 42 as well as reverse primers consisting of the base sequences of SEQ ID NOs: 13 or 91, 29 and 48, respectively. The use of these primer sets makes it possible to amplify three target regions including parts where three types of polymorphisms (UGT1A1*6, UGT1A1*27, and UGT1A1*28) of the UGT1A1 gene are generated, respectively, in the same reaction solution at the same time.
摘要翻译：提供了通过基因扩增方法扩增含有在UGT1A1基因中检测的位点的靶区的引物组，其中引物组可以特异性地扩增区域。使用三对引物组，包括由SEQ ID NO：4或81,21和42以及分别由SEQ ID NO：13或91,29和48的碱基序列组成的反向引物。这些引物组的使用使得可以扩增三个靶区，包括分别在相同的反应溶液中产生UGT1A1基因的三种类型的多态性（UGT1A1 * 6，UGT1A1 * 27和UGT1A1 * 28）的部分同一时间。

7. 发明申请

US20110244460A1 METHOD FOR DETECTING CONTROLS FOR NUCLEIC ACID AMPLIFICATION AND USE THEREOF 审中-公开
标题翻译：用于检测核酸扩增控制的方法及其用途
公开(公告)号：US20110244460A1
公开(公告)日：2011-10-06
申请号：US13139290
申请日：2009-12-16
申请人： Mitsuharu Hirai , Satoshi Majima , Shinya Nakajima , Tatsuo Kamata
发明人： Mitsuharu Hirai , Satoshi Majima , Shinya Nakajima , Tatsuo Kamata
IPC分类号： C12Q1/68 , C07H21/00 , G06G7/58
CPC分类号： C12Q1/6848 , C12Q2545/107 , C12Q2527/107 , C12Q2545/101
摘要： The present invention provides a control detection method for easily detecting a positive control and a negative control simultaneously in one reaction system. An amplification reaction is carried out by adding a control template nucleic acid to a reaction system for detecting controls. The template nucleic acid can be amplified by a primer capable of amplifying an objective target sequence. An amplification region of the control template nucleic acid amplified by the primer can be hybridized with a detection probe capable of hybridizing to the target sequence. A Tm value (TmC) of a double-stranded nucleic acid composed of the amplification region of the control template nucleic acid amplified by the primer and the detection probe is set different from a Tm value (TmA) of a double-stranded nucleic acid composed of the target sequence and the detection probe. Thereby, it can be determined whether or not amplification occurs in a reaction system on the basis of the presence or absence of a peak at the TmC and it can be determined whether or not a reaction system is contaminated with undesired nucleic acid on the basis of the presence or absence of a peak at a temperature other than TmC.
摘要翻译：本发明提供一种用于在一个反应系统中同时容易地检测阳性对照和阴性对照的控制检测方法。通过将对照模板核酸加入到用于检测对照的反应系统中进行扩增反应。模板核酸可以通过能够扩增目的靶序列的引物扩增。由引物扩增的对照模板核酸的扩增区可以与能够与靶序列杂交的检测探针杂交。将由引物扩增的对照模板核酸的扩增区和检测用探针构成的双链核酸的Tm值（TmC）设定为与构成的双链核酸的Tm值（TmA）不同的目标序列和检测探针。因此，可以基于在TmC的峰的存在或不存在来确定反应体系中是否发生扩增，并且可以基于以下方式确定反应体系是否被不期望的核酸污染在TmC以外的温度下存在或不存在峰。

8. 发明授权

US08357516B2 Primer set for amplification of UGT1A1 gene, reagent for amplification of UGT1A1 gene containing the same, and the uses thereof 有权
标题翻译：用于扩增UGT1A1基因的引物，用于扩增含有该UGT1A1基因的UGT1A1基因的试剂及其用途
公开(公告)号：US08357516B2
公开(公告)日：2013-01-22
申请号：US12293954
申请日：2007-11-29
申请人： Mitsuharu Hirai , Satoshi Majima
发明人： Mitsuharu Hirai , Satoshi Majima
IPC分类号： C12P19/34
CPC分类号： C12N9/1051 , C12Q1/6886 , C12Q2600/156 , C12Q2600/16
摘要： Primer sets for amplifying target regions containing sites to be detected in the UGT1A1 gene by a gene amplification method are provided, wherein the primer sets can amplify the regions specifically. Three pairs of primer sets are used including forward primers consisting of the base sequences of SEQ ID NOs: 4 or 81, 21, and 42 as well as reverse primers consisting of the base sequences of SEQ ID NOs: 13 or 91, 29 and 48, respectively. The use of these primer sets makes it possible to amplify three target regions including parts where three types of polymorphisms (UGT1A1*6, UGT1A1*27, and UGT1A1*28) of the UGT1A1 gene are generated, respectively, in the same reaction solution at the same time.
摘要翻译：提供了通过基因扩增方法扩增含有UGT1A1基因中待检测位点的靶区的引物组，其中引物组可以特异性扩增该区域。使用三对引物组，包括由SEQ ID NO：4或81,21和42的碱基序列组成的正向引物，以及由SEQ ID NO：13或91,29和48的碱基序列组成的反向引物，分别。这些引物组的使用使得可以扩增三个靶区，包括分别在相同的反应溶液中产生UGT1A1基因的三种类型的多态性（UGT1A1 * 6，UGT1A1 * 27和UGT1A1 * 28）的部分同一时间。

9. 发明申请

US20110287421A1 Probes for Detection of NAT2 Gene, Reagent Containing the Same, and The Uses Thereof 审中-公开
标题翻译：用于检测NAT2基因的探针，含有其的试剂及其用途
公开(公告)号：US20110287421A1
公开(公告)日：2011-11-24
申请号：US13084919
申请日：2011-04-12
申请人： Mitsuharu Hirai , Satoshi Majima
发明人： Mitsuharu Hirai , Satoshi Majima
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6876 , C12Q2600/156 , C12Q2600/16
摘要： Primer sets for amplifying target regions containing sites to be detected in the NAT2 gene by a gene amplification method are provided, wherein the primer sets can amplify the regions specifically. Three pairs of primer sets are used including forward primers consisting of the base sequences of SEQ ID NOs: 7, 33, and 60 as well as reverse primers consisting of the base sequences of SEQ ID NOs: 18, 48 and 81, respectively. The use of these primer sets makes it possible to amplify three target regions including parts where three types of polymorphisms (NAT2*5, NAT2*6, and NAT2*7) of the NAT2 gene are generated, respectively, in the same reaction solution at the same time.
摘要翻译：提供了通过基因扩增方法扩增含有待检测NAT2基因的位点的靶区域的引物组，其中引物组可以特异性地扩增该区域。使用三对引物组，包括由SEQ ID NO：7,33和60的碱基序列组成的正向引物，以及分别由SEQ ID NO：18,48和81的碱基序列组成的反向引物。这些引物组的使用使得可以扩增三个靶区，包括分别在同一反应溶液中产生NAT2基因的三种类型的多态性（NAT2 * 5，NAT2 * 6和NAT2 * 7）的部分同一时间。

10. 发明申请

US20100297617A1 PRIMER SET FOR AMPLIFYING NAT2 GENE, REAGENT FOR AMPLIFYING NAT2 GENE CONTAINING THE SAME, AND THE USES THEREOF 审中-公开
标题翻译：用于放大NAT2基因的引物组，用于扩增含有其的NAT2基因的试剂及其用途
公开(公告)号：US20100297617A1
公开(公告)日：2010-11-25
申请号：US12297157
申请日：2007-11-30
申请人： Mitsuharu Hirai , Satoshi Majima
发明人： Mitsuharu Hirai , Satoshi Majima
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q2600/156 , C12Q2600/16
摘要： Primer sets for amplifying target regions containing sites to be detected in the NAT2 gene by a gene amplification method are provided, wherein the primer sets can amplify the regions specifically. Three pairs of primer sets are used including forward primers consisting of the base sequences of SEQ ID NOs: 7, 33, and 60 as well as reverse primers consisting of the base sequences of SEQ ID NOs: 18, 48 and 81, respectively. The use of these primer sets makes it possible to amplify three target regions including parts where three types of polymorphisms (NAT2*5, NAT2*6, and NAT2*7) of the NAT2 gene are generated, respectively, in the same reaction solution at the same time.
摘要翻译：提供了通过基因扩增方法扩增含有待检测NAT2基因的位点的靶区域的引物组，其中引物组可以特异性地扩增该区域。使用三对引物组，包括由SEQ ID NO：7,33和60的碱基序列组成的正向引物，以及分别由SEQ ID NO：18,48和81的碱基序列组成的反向引物。这些引物组的使用使得可以扩增三个靶区，包括分别在同一反应溶液中产生NAT2基因的三种类型的多态性（NAT2 * 5，NAT2 * 6和NAT2 * 7）的部分同一时间。

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