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1. 发明授权

US09045799B2 Probe for detecting polymorphism in CYP3A gene, method of detecting polymorphism, method of evaluating drug efficacy, and reagent kit for detecting polymorphism 有权
标题翻译：检测CYP3A基因多态性的探针，多态性检测方法，药物疗效评价方法及多态性检测试剂盒
公开(公告)号：US09045799B2
公开(公告)日：2015-06-02
申请号：US13416357
申请日：2012-03-09
申请人： Aki Iguchi , Mitsuharu Hirai
发明人： Aki Iguchi , Mitsuharu Hirai
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02
CPC分类号： C12Q1/6883 , C12Q1/6876 , C12Q2600/112 , C12Q2600/156
摘要： Provided in the present disclosure is a probe for detecting polymorphism that enables a simple detection of polymorphism in the CYP3A gene with high sensitivity.
摘要翻译：在本公开中提供了用于检测多态性的探针，其能够以高灵敏度简单检测CYP3A基因的多态性。

2. 再颁专利

USRE44894E1 Method of detecting or quantitatively determining mitochondrial DNA 3243 variation, and kit therefor 有权
标题翻译：检测或定量测定线粒体DNA 3243变异的方法及其试剂盒
公开(公告)号：USRE44894E1
公开(公告)日：2014-05-13
申请号：US13234907
申请日：2011-09-16
申请人： Mitsuharu Hirai
发明人： Mitsuharu Hirai
IPC分类号： C12Q1/68 , C12P19/34
摘要： A method for detecting a DNA having the mitochondrial DNA 3243 mutation is disclosed. Quantitative PCR is used with a primer having a nucleotide sequence complementary to the nucleotide sequence starting from the nucleotide number 243 in SEQ ID NO: 2 and having a length of 12 to 30 nucleotides. A method is also disclosed for detecting a DNA having the mitochondrial DNA 3243 mutation by using a nucleic acid probe which is end labeled with a fluorescent dye. The fluorescence of the fluorescent dye decreases upon hybridization. The nucleic acid probe has a nucleotide sequence complementary to the nucleotide sequence starting from nucleotide number 230 in the nucleotide sequence of SEQ ID NO: 2 and a length of 14 to 40 nucleotides. The 3′ end of the probe is labeled with the fluorescent dye.
摘要翻译：公开了一种用于检测具有线粒体DNA 3243突变的DNA的方法。定量PCR与具有与核苷酸序列互补的核苷酸序列的引物使用，其核苷酸序列以SEQ ID NO：2的核苷酸编号243开始，长度为12〜30个核苷酸。还公开了通过使用用荧光染料末端标记的核酸探针来检测具有线粒体DNA 3243突变的DNA的方法。荧光染料的荧光在杂交时降低。核酸探针具有与SEQ ID NO：2的核苷酸序列中的核苷酸230号开始的核苷酸序列互补的核苷酸序列，长度为14〜40个核苷酸。探针的3'末端用荧光染料标记。

3. 发明申请

US20120270215A1 PROBE FOR DETECTING POLYMORPHISM IN CYP3A GENE, METHOD OF DETECTING POLYMORPHISM, METHOD OF EVALUATING DRUG EFFICACY, AND REAGENT KIT FOR DETECTING POLYMORPHISM 有权
标题翻译：用于检测CYP3A基因多态性的检测方法，检测多态性的方法，评估药物效能的方法以及用于检测多态性的试剂盒
公开(公告)号：US20120270215A1
公开(公告)日：2012-10-25
申请号：US13416357
申请日：2012-03-09
申请人： Aki Iguchi , Mitsuharu Hirai
发明人： Aki Iguchi , Mitsuharu Hirai
IPC分类号： C07H21/00 , C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6876 , C12Q2600/112 , C12Q2600/156
摘要： Provided in the present disclosure is a probe for detecting polymorphism that enables a simple detection of polymorphism in the CYP3A gene with high sensitivity.
摘要翻译：在本公开中提供了用于检测多态性的探针，其能够以高灵敏度简单检测CYP3A基因的多态性。

4. 发明申请

US20120231463A1 Primer Set for Amplification of MTHFR Gene, MTHFR Gene Amplification Reagent Containing the Same, and Use of the Same 审中-公开
标题翻译：用于扩增MTHFR基因的引物，包含其的MTHFR基因扩增试剂及其用途
公开(公告)号：US20120231463A1
公开(公告)日：2012-09-13
申请号：US13510523
申请日：2010-11-19
申请人： Mitsuharu Hirai , Masahiro Kozuka
发明人： Mitsuharu Hirai , Masahiro Kozuka
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6883 , C12Q2600/156
摘要： The present invention provides a primer set for specifically amplifying a target region in a MTHFR gene by a nucleic acid amplification method, a MTHFR gene amplification reagent containing the primer set, and use of the primer set.
摘要翻译：本发明提供了通过核酸扩增法特异性扩增MTHFR基因中的靶区域的引物组，含有引物组的MTHFR基因扩增试剂和引物组的使用。

5. 发明授权

US08232051B2 Primer set for gene amplification, reagent for gene amplification including the same, and uses thereof 有权
标题翻译：用于基因扩增的引物，用于包含其的基因扩增用试剂及其用途
公开(公告)号：US08232051B2
公开(公告)日：2012-07-31
申请号：US12306370
申请日：2008-03-24
申请人： Mitsuharu Hirai , Satoshi Majima
发明人： Mitsuharu Hirai , Satoshi Majima
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6883 , C12Q2600/156 , C12Q2600/16
摘要： Primer sets for amplifying two genes (the CYP2C9 gene and the VKORC1 gene) by a gene amplification method are provided, wherein the primer sets can amplify respective target regions of the two genes specifically and efficiently in the same reaction system simultaneously. Two pairs of primer sets are used including forward primers consisting of the base sequences of SEQ ID NOs: 5 and 29 as well as reverse primers consisting of the base sequences of SEQ ID NOs: 18 and 38, respectively. The use of these primer sets makes it possible to specifically amplify target regions including sites where polymorphisms to be detected are generated in the CYP2C9 gene and the VKORC1 gene, in the same reaction solution simultaneously.
摘要翻译：提供了通过基因扩增方法扩增两个基因（CYP2C9基因和VKORC1基因）的引物组，其中引物可以同时在同一反应体系中特异性和有效地扩增两个基因的各个靶区域。使用两对引物组，包括由SEQ ID NO：5和29的碱基序列组成的正向引物，以及分别由SEQ ID NO：18和38的碱基序列组成的反向引物。这些引物组的使用使得可以同时在相同的反应溶液中特异性扩增包括在CYP2C9基因和VKORC1基因中产生待检测多态性的位点的靶区域。

6. 发明申请

US20110281265A1 Primer set for amplifying UGT1A1 gene, reagent for amplifying UGT1A1 gene containing the same, and the uses thereof 审中-公开
标题翻译：用于扩增UGT1A1基因的引物，用于扩增含有其的UGT1A1基因的试剂及其用途
公开(公告)号：US20110281265A1
公开(公告)日：2011-11-17
申请号：US13078285
申请日：2011-04-01
申请人： Mitsuharu Hirai , Satoshi Majima
发明人： Mitsuharu Hirai , Satoshi Majima
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N9/1051 , C12Q1/6886 , C12Q2600/156 , C12Q2600/16
摘要： Primer sets for amplifying target regions containing sites to be detected in the UGT1A1 gene by a gene amplification method are provided, wherein the primer sets can amplify the regions specifically Three pairs of primer sets are used including forward primers consisting of the base sequences of SEQ ID NOs: 4 or 81, 21, and 42 as well as reverse primers consisting of the base sequences of SEQ ID NOs: 13 or 91, 29 and 48, respectively. The use of these primer sets makes it possible to amplify three target regions including parts where three types of polymorphisms (UGT1A1*6, UGT1A1*27, and UGT1A1*28) of the UGT1A1 gene are generated, respectively, in the same reaction solution at the same time.
摘要翻译：提供了通过基因扩增方法扩增含有在UGT1A1基因中检测的位点的靶区的引物组，其中引物组可以特异性地扩增区域。使用三对引物组，包括由SEQ ID NO：4或81,21和42以及分别由SEQ ID NO：13或91,29和48的碱基序列组成的反向引物。这些引物组的使用使得可以扩增三个靶区，包括分别在相同的反应溶液中产生UGT1A1基因的三种类型的多态性（UGT1A1 * 6，UGT1A1 * 27和UGT1A1 * 28）的部分同一时间。

7. 发明授权

US08021845B2 Probes for detecting obesity gene 有权
标题翻译：检测肥胖基因的探针
公开(公告)号：US08021845B2
公开(公告)日：2011-09-20
申请号：US12306417
申请日：2007-11-30
申请人： Mitsuharu Hirai , Satoshi Majima
发明人： Mitsuharu Hirai , Satoshi Majima
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6883 , C12Q2600/156 , C12Q2600/16
摘要： Primer sets for amplifying target regions containing sites to be detected in the obesity gene (the β2AR gene, the β3AR gene, and the UCP1 gene) by a gene amplification method are provided, wherein the primer sets can amplify the regions specifically. Three pairs of primer sets are used including forward primers composed of the base sequences of SEQ ID NO: 9 or SEQ ID NO: 109, SEQ ID NO: 25, and SEQ ID NO:43 as well as reverse primers composed of the base sequences of SEQ ID NO: 18, SEQ ID NO: 30, and SEQ ID NO: 63, respectively. The use of these primer sets makes it possible to specifically amplify a target region including a site where a polymorphism to be detected is generated in the β2AR gene, the β3AR gene, and the UCP1 gene, in the same reaction solution at the same time.
摘要翻译：提供了通过基因扩增方法扩增含有在肥胖基因中检测到的位点（“bgr2AR基因”，“3AR基因”，“UCP1基因”）的靶区域的引物组，其特征在于，。使用三对引物组，包括由SEQ ID NO：9或SEQ ID NO：109，SEQ ID NO：25和SEQ ID NO：43的碱基序列组成的正向引物，以及由碱基序列组成的反向引物分别为SEQ ID NO：18，SEQ ID NO：30和SEQ ID NO：63。这些引物组的使用使得可以特异性地扩增包含在第2AR基因，第3AR基因和UCP1基因中产生待检测多态性的位点的靶区域，在同一反应溶液中同一时间。

8. 发明申请

US20100068713A1 PROBE FOR DETECTING MUTATION IN JAK2 GENE AND USE THEREOF 审中-公开
标题翻译：用于检测JAK2基因中的突变的探针及其用途
公开(公告)号：US20100068713A1
公开(公告)日：2010-03-18
申请号：US12513642
申请日：2008-07-11
申请人： Mitsuharu Hirai , Satoshi Majima , Taira Maekawa , Shinya Kimura
发明人： Mitsuharu Hirai , Satoshi Majima , Taira Maekawa , Shinya Kimura
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/04
CPC分类号： C12Q1/6883 , C12Q2600/156 , Y10T436/143333
摘要： A probe for detecting a mutation in the JAK2 gene is provided that can detect a target sequence containing a mutation even when the target sequence containing the mutation and a non-target sequence containing no mutation coexist, which are different only in a single base from each other. The probe to be used is an oligonucleotide that is at least one oligonucleotide having a sequence identical to that of a region extending from a cytosine base (C) at position 84 to be considered as the first base to any one of the 17th to 22nd bases in the direction toward the 5′ end in exon 12 of the JAK2 gene consisting of the base sequence of SEQ ID NO: 1, with the cytosine base (C) being the 3′ end. Even in the case of a sample containing both the JAK2 genes with a mutation that has occurred and without a mutation that has occurred, the use of such a probe in, for example, Tm analysis allows the former mutation to be detected. Preferably, the probe is labeled with a fluorescent dye.
摘要翻译：提供了用于检测JAK2基因突变的探针，即使当含有突变的靶序列和不含突变的非靶序列共存时也可以检测到含有突变的靶序列，其仅在单个碱基中不同其他。要使用的探针是至少一种寡核苷酸，其具有与从第84位的胞嘧啶碱基（C）延伸的区域的序列相同的寡核苷酸，被认为是第17位至第22位碱基的第一碱基在由SEQ ID NO：1的碱基序列组成的JAK2基因的外显子12的5'端的方向上，胞嘧啶碱基（C）为3'末端。即使在含有具有发生突变且没有发生突变的JAK2基因的样品的情况下，在例如Tm分析中使用这种探针也可以检测到前述突变。优选地，探针用荧光染料标记。

9. 发明申请

US20100047806A1 PROBES FOR DETECTING IMMUNE-RELATED GENE POLYMORPHISMS AND APPLICATIONS OF THE SAME 有权
标题翻译：用于检测与免疫相关基因多态性及其应用的探针
公开(公告)号：US20100047806A1
公开(公告)日：2010-02-25
申请号：US12594519
申请日：2008-10-03
申请人： Mitsuharu Hirai , Satoshi Majima
发明人： Mitsuharu Hirai , Satoshi Majima
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q2600/156 , C12Q2527/107
摘要： Polymorphism detection probes that can distinguish polymorphisms that have only one different base are provided. At least one oligonucleotide selected from the group consisting of the oligonucleotides of SEQ ID NOS. 4, 23, 30, 47, 57 and 64 is used as a probe in a Tm analysis. A Tm analysis using such probes allows easy detection of specific polymorphisms of the FCGR3A gene, the FCGR2A gene, the IL-10 gene, the TNF α gene and the TNF 8 gene that have an effect on the pharmaceutical effects of antibody drugs or the like. Moreover, such probes allow detection of two or more types of polymorphisms in a single reaction system by introducing two or more types of the probes concomitantly.
摘要翻译：提供可以区分只有一个不同基数的多态性的多态性检测探针。至少一种选自下组的寡核苷酸：SEQ ID NO：在Tm分析中使用4,23,30,47,57和64作为探针。使用这种探针的Tm分析允许容易地检测对抗体药物等的药物作用有影响的FCGR3A基因，FCGR2A基因，IL-10基因，TNFα基因和TNF8基因的特异性多态性。此外，这样的探针允许通过同时引入两种或更多种类型的探针来在单个反应系统中检测两种或更多种类型的多态性。

10. 发明申请

US20090208954A1 PRIMER SET FOR AMPLIFICATION OF UGT1A1 GENE, REAGENT FOR AMPLIFICATION OF UGT1A1 GENE CONTAINING THE SAME, AND THE USES THEREOF 有权
标题翻译：用于放大UGT1A1基因的引物组，用于扩增含有其的UGT1A1基因的试剂及其用途
公开(公告)号：US20090208954A1
公开(公告)日：2009-08-20
申请号：US12293954
申请日：2007-11-29
申请人： Mitsuharu Hirai , Satoshi Majima
发明人： Mitsuharu Hirai , Satoshi Majima
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12N9/1051 , C12Q1/6886 , C12Q2600/156 , C12Q2600/16
摘要： Primer sets for amplifying target regions containing sites to be detected in the UGT1A1 gene by a gene amplification method are provided, wherein the primer sets can amplify the regions specifically. Three pairs of primer sets are used including forward primers consisting of the base sequences of SEQ ID NOs: 4 or 81, 21, and 42 as well as reverse primers consisting of the base sequences of SEQ ID NOs: 13 or 91, 29 and 48, respectively. The use of these primer sets makes it possible to amplify three target regions including parts where three types of polymorphisms (UGT1A1*6, UGT1A1*27, and UGT1A1*28) of the UGT1A1 gene are generated, respectively, in the same reaction solution at the same time.
摘要翻译：提供了通过基因扩增方法扩增含有UGT1A1基因中待检测位点的靶区的引物组，其中引物组可以特异性扩增该区域。使用三对引物组，包括由SEQ ID NO：4或81,21和42的碱基序列组成的正向引物，以及由SEQ ID NO：13或91,29和48的碱基序列组成的反向引物，分别。这些引物组的使用使得可以扩增三个靶区，包括分别在相同的反应溶液中产生UGT1A1基因的三种类型的多态性（UGT1A1 * 6，UGT1A1 * 27和UGT1A1 * 28）的部分同一时间。

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