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    • 3. 发明授权
    • Method for forming artificial lipid membrane
    • 人造脂膜形成方法
    • US08062489B2
    • 2011-11-22
    • US12971885
    • 2010-12-17
    • Akio OkiNorihito TsukaharaMasato SuzukiHiroaki Oka
    • Akio OkiNorihito TsukaharaMasato SuzukiHiroaki Oka
    • G01N27/327G01N33/92B01L3/00
    • G01N33/48728
    • An object of the present invention is to provide a method for stably forming an artificial lipid membrane while suppressing the leakage and evaporation of an electrolytic solution. The present invention is an artificial lipid membrane forming method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus. The artificial lipid membrane forming apparatus comprises a first chamber, a second chamber, a dividing wall, and an artificial lipid membrane forming portion. Each of the first chamber and the second chamber has a capacity of not smaller than 10 pl and not larger than 200 μl. The artificial lipid membrane forming method of the present invention comprises the steps of: preparing the artificial lipid membrane forming apparatus; adding to the first chamber a first electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; adding a lipid solution to the artificial lipid membrane forming portion; adding to the second chamber a second electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; and forming the artificial lipid membrane.
    • 本发明的目的是提供一种在抑制电解液的泄漏和蒸发的同时稳定地形成人造脂膜的方法。 本发明是使用人造脂质膜形成装置形成人造脂质膜的人造脂质膜形成方法。 人造脂膜形成装置包括第一室,第二室,分隔壁和人造脂膜形成部。 第一室和第二室中的每一个具有不小于10pl且不大于200μl的容量。 本发明的人造脂膜形成方法包括以下步骤:制备人造脂膜形成装置; 向第一室中加入粘度不低于1.3mPa·s且不高于200mPa·s的第一电解液; 向人造脂膜形成部分添加脂质溶液; 向第二室中加入粘度不低于1.3mPa·s且不高于200mPa·s的第二电解液; 并形成人造脂膜。
    • 4. 发明申请
    • METHOD FOR FORMING ARTIFICIAL LIPID MEMBRANE
    • 形成人造脂膜的方法
    • US20110083793A1
    • 2011-04-14
    • US12971885
    • 2010-12-17
    • Akio OKINorihito TsukaharaMasato SuzukiHiroaki Oka
    • Akio OKINorihito TsukaharaMasato SuzukiHiroaki Oka
    • B29C39/02C25F3/14B05D1/04B05D5/00B32B38/00
    • G01N33/48728
    • An object of the present invention is to provide a method for stably forming an artificial lipid membrane while suppressing the leakage and evaporation of an electrolytic solution.The present invention is an artificial lipid membrane forming method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus. The artificial lipid membrane forming apparatus comprises a first chamber, a second chamber, a dividing wall, and an artificial lipid membrane forming portion. Each of the first chamber and the second chamber has a capacity of not smaller than 10 pl and not larger than 200 μl. The artificial lipid membrane forming method of the present invention comprises the steps of: preparing the artificial lipid membrane forming apparatus; adding to the first chamber a first electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; adding a lipid solution to the artificial lipid membrane forming portion; adding to the second chamber a second electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; and forming the artificial lipid membrane.
    • 本发明的目的是提供一种在抑制电解液的泄漏和蒸发的同时稳定地形成人造脂膜的方法。 本发明是使用人造脂质膜形成装置形成人造脂质膜的人造脂质膜形成方法。 人造脂膜形成装置包括第一室,第二室,分隔壁和人造脂膜形成部。 第一室和第二室中的每一个具有不小于10pl且不大于200μl的容量。 本发明的人造脂膜形成方法包括以下步骤:制备人造脂膜形成装置; 向第一室中加入粘度不低于1.3mPa·s且不高于200mPa·s的第一电解液; 向人造脂膜形成部分添加脂质溶液; 向第二室中加入粘度不低于1.3mPa·s且不高于200mPa·s的第二电解液; 并形成人造脂膜。