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1. 发明授权

US08062489B2 Method for forming artificial lipid membrane 有权
标题翻译：人造脂膜形成方法
公开(公告)号：US08062489B2
公开(公告)日：2011-11-22
申请号：US12971885
申请日：2010-12-17
申请人： Akio Oki , Norihito Tsukahara , Masato Suzuki , Hiroaki Oka
发明人： Akio Oki , Norihito Tsukahara , Masato Suzuki , Hiroaki Oka
IPC分类号： G01N27/327 , G01N33/92 , B01L3/00
CPC分类号： G01N33/48728
摘要： An object of the present invention is to provide a method for stably forming an artificial lipid membrane while suppressing the leakage and evaporation of an electrolytic solution. The present invention is an artificial lipid membrane forming method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus. The artificial lipid membrane forming apparatus comprises a first chamber, a second chamber, a dividing wall, and an artificial lipid membrane forming portion. Each of the first chamber and the second chamber has a capacity of not smaller than 10 pl and not larger than 200 μl. The artificial lipid membrane forming method of the present invention comprises the steps of: preparing the artificial lipid membrane forming apparatus; adding to the first chamber a first electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; adding a lipid solution to the artificial lipid membrane forming portion; adding to the second chamber a second electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; and forming the artificial lipid membrane.
摘要翻译：本发明的目的是提供一种在抑制电解液的泄漏和蒸发的同时稳定地形成人造脂膜的方法。本发明是使用人造脂质膜形成装置形成人造脂质膜的人造脂质膜形成方法。人造脂膜形成装置包括第一室，第二室，分隔壁和人造脂膜形成部。第一室和第二室中的每一个具有不小于10pl且不大于200μl的容量。本发明的人造脂膜形成方法包括以下步骤：制备人造脂膜形成装置; 向第一室中加入粘度不低于1.3mPa·s且不高于200mPa·s的第一电解液; 向人造脂膜形成部分添加脂质溶液; 向第二室中加入粘度不低于1.3mPa·s且不高于200mPa·s的第二电解液; 并形成人造脂膜。

2. 发明授权

US08030059B2 Apparatus for measuring extracellular potential 有权
标题翻译：用于测量细胞外电位的装置
公开(公告)号：US08030059B2
公开(公告)日：2011-10-04
申请号：US12133461
申请日：2008-06-05
申请人： Masaya Nakatani , Nobuhiko Ozaki , Hiroaki Oka
发明人： Masaya Nakatani , Nobuhiko Ozaki , Hiroaki Oka
IPC分类号： C12M1/34
CPC分类号： G01N33/48728
摘要： A sensing element for measuring extracellular potential including a substrate, a well provided in a substrate, a guide section provided on the wall of the well, and a detective electrode formed at a lower surface of the substrate. The guide section is for guiding drug. The well is provided at the bottom with a depression, and a first throughhole penetrating through the depression and the lower surface of the substrate. The well is for mixing a subject cell, a culture solution and the drug together. The above-configured sensing element accurately measures a change generated by a subject cell.
摘要翻译：用于测量细胞外电位的感测元件，包括衬底，设置在衬底中的阱，设置在阱的壁上的引导部分和形成在衬底的下表面处的检测电极。指导部分是指导药物。井在底部设置有凹陷，并且第一通孔穿过基底的凹陷和下表面。该井用于将主体细胞，培养液和药物混合在一起。上述构成的感测元件精确地测量由被摄体单元产生的变化。

3. 发明申请

US20100147682A1 PROBE FOR MEASURING ELECTRIC POTENTIAL OF CELL 审中-公开
标题翻译：用于测量细胞电位的探针
公开(公告)号：US20100147682A1
公开(公告)日：2010-06-17
申请号：US12712370
申请日：2010-02-25
申请人： Masaya Nakatani , Nobuhiko Ozaki , Hiroaki Oka
发明人： Masaya Nakatani , Nobuhiko Ozaki , Hiroaki Oka
IPC分类号： G01N27/26
CPC分类号： G01N33/48728
摘要： A probe for measuring an electric potential of a cell includes a plate having a surface having a first cavity provided therein, and a sensor element provided in the first cavity. A second cavity is provided in the bottom surface of the first cavity. The first flow passage having first and second openings is provided in the plate. The first and second openings of the first flow passage open to the second cavity and outside the plate, respectively. The sensor element includes a thin plate, and a supporting substrate provided around the thin plate and in the first cavity of the plate. The thin plate has a through-hole therein having a first opening and a second opening communicating with the second cavity of the plate. The first flow passage allows fluid to flow therein. A sucking device is coupled with the second opening of the first flow passage as to suck the fluid flowing in the first flow passage. This probe can measure an electric potential of a cell floating in solution as it is in this environment.
摘要翻译：用于测量电池的电位的探针包括具有设置在其中的具有第一腔的表面的板和设置在第一腔中的传感器元件。在第一腔的底表面中设置第二腔。具有第一和第二开口的第一流动通道设置在板中。第一流动通道的第一和第二开口分别向第二腔室和板外开口。传感器元件包括薄板，以及设置在薄板周围和板的第一腔中的支撑基板。薄板的通孔中具有第一开口和与板的第二腔连通的第二开口。第一流道允许流体流入其中。抽吸装置与第一流动通道的第二开口联接以吸收在第一流动通道中流动的流体。该探头可以在这种环境中测量浮动在溶液中的电池的电位。

4. 发明申请

US20100137742A1 SKIN INCISION INSTRUMENT AND METHOD FOR INCISING SKIN WITH THE SAME 有权
公开(公告)号：US20100137742A1
公开(公告)日：2010-06-03
申请号：US12699538
申请日：2010-02-03
申请人： Akio OKI , Hiroaki Oka
发明人： Akio OKI , Hiroaki Oka
IPC分类号： A61B17/3209 , A61B5/15
CPC分类号： A61B5/15186 , A61B5/150022 , A61B5/150068 , A61B5/150335 , A61B5/150389 , A61B5/150427 , A61B5/150442 , A61B5/150503 , A61B5/150541 , A61B5/150618 , A61B5/150717 , A61B5/15113 , A61B5/15117 , A61B5/1519 , A61B5/157 , A61B5/411
摘要： Provided are skin incision instrument to efficiently incise minimal portions and a method for incising skin with the skin incision instrument. The skin incision instrument according to the present invention comprises a holder, a needle, a needle drive unit, two or more skin expander sets, and a reader, wherein the skin expander sets comprise a first skin expander and a second skin expander; the first skin expander and the second skin expander are capable of expanding skin at both sides of the linear incision portion away from the linear incision portion and expanding the linear incision portion in a direction to expand the linear incision portion; and, minimal portions are efficiently incised by selecting a single skin expander set from two or more of the skin expander set based on the direction of the linear incision portion, such that an angle is adjusted to 45 degrees or more and 90 degrees or less wherein the angle is formed by the direction of the linear incision portion read by the reader and the direction to expand the skin by the single skin expander set.

5. 发明授权

US07678249B2 Instrument and system for pharmacologic measurement and well vessel used therein 有权
标题翻译：用于药物测量的仪器和系统及其中使用的井筒
公开(公告)号：US07678249B2
公开(公告)日：2010-03-16
申请号：US11213961
申请日：2005-08-30
申请人： Nobuhiko Ozaki , Hiroaki Oka
发明人： Nobuhiko Ozaki , Hiroaki Oka
IPC分类号： G01N33/487
CPC分类号： G01N33/48728
摘要： An instrument is provided for pharmacologic measurement capable of detecting a very small and short time change in electric signal caused by a pharmacologic action of a biologic specimen with quickness and a high sensitivity by steeply decreasing an external disturbance component mixed into the system while dropping/exchange of medicinal solutions is conducted in a batch mode.The pharmacologic measurement instrument detects a change in electric signal caused by a pharmacologic action or electrophysiologic action of a biologic specimen, and includes an electrically conductive box having an opening section at the top surface thereof, and a well vessel disposed in the opening section thereof, wherein the well vessel includes a base having recesses in which the biologic specimens are put; measurement electrodes formed on the bottom surfaces of the respective recesses; and reference electrodes electrically insulated from the respective measurement electrodes, and the reference electrodes together with the electrically conductive box electrostatically shields the well vessel.
摘要翻译：提供了用于药物测量的仪器，其能够通过快速和高灵敏度检测由生物样品的药理作用引起的电信号的非常小的和短的时间变化，通过急剧降低混合到系统中的外部干扰成分，同时进行下降/交换药物溶液以批式进行。药理学测量仪器检测由生物样本的药理作用或电生理作用引起的电信号变化，并且包括在其顶表面具有开口部分的导电盒和设置在其开口部分中的井管，其中所述井管包括具有其中放置生物样本的凹部的基部; 形成在各个凹部的底面上的测量电极; 以及与各测量电极电绝缘的参考电极，并且参考电极与导电盒一起静电屏蔽阱容器。

6. 发明授权

US07594984B2 Apparatus and method for measuring activity signals of biological samples 有权
标题翻译：用于测量生物样品活动信号的装置和方法
公开(公告)号：US07594984B2
公开(公告)日：2009-09-29
申请号：US11652605
申请日：2007-01-12
申请人： Hiroaki Oka , Nobuhiko Ozaki , Hirokazu Sugihara
发明人： Hiroaki Oka , Nobuhiko Ozaki , Hirokazu Sugihara
IPC分类号： G01N33/50 , G01N27/00 , G01N33/543 , C12Q1/00 , G01N17/04
CPC分类号： G01N33/48728 , G01N33/54373 , G01N33/5438
摘要： The present invention provides an apparatus for measuring activity signals of a biological sample comprising: a measurement chamber (A) storing a target liquid containing a biological sample; a porous insulating substrate (5) provided with a measurement electrode (1) on at least one side; and a conveying device (8) which conveys the target liquid stored in the measurement chamber (A) and passes the target liquid through the porous insulating substrate (5) from the measurement electrode (1) side, wherein the conveying device (8) is operated to trap the biological sample contained in the target liquid onto the measurement electrode (1), so that the activity signals of the biological sample are measured through the measurement electrode (1). According to the apparatus, activity signals emitted from the biological sample can be detected easily, rapidly and accurately.
摘要翻译：本发明提供了一种用于测量生物样品的活动信号的装置，包括：存储含有生物样品的目标液体的测量室（A）在至少一侧设置有测量电极（1）的多孔绝缘基板（5）以及输送装置（8），其输送存储在测量室（A）中的目标液体，并将目标液体通过多孔绝缘基板（5）从测量电极（1）侧移动，其中输送装置（8）为操作以将包含在目标液体中的生物样品捕获到测量电极（1）上，从而通过测量电极（1）测量生物样品的活性信号。根据该装置，可以容易，快速且准确地检测从生物样品发射的活动信号。

7. 发明申请

US20080293128A1 SENSOR OF PYROPHOSPHATE AND SNP TYPING SENSOR USING THE SAME 有权
标题翻译：使用相同的磷酸酯和SNP型传感器的传感器
公开(公告)号：US20080293128A1
公开(公告)日：2008-11-27
申请号：US12112249
申请日：2008-04-30
申请人： Tetsuo YUKIMASA , Hiroaki Oka
发明人： Tetsuo YUKIMASA , Hiroaki Oka
IPC分类号： C12M1/00
CPC分类号： C12Q1/6827 , C12Q2565/301
摘要： A sensor of pyrophosphate which can detect pyrophosphate conveniently with high sensitivity in a method for measuring pyrophosphate in SNP typing utilizing a primer extension reaction is provided.A sensor of pyrophosphate which is characterized by including: an insulative substrate 1; an electrode system that is formed thereon and has a measurement electrode 2 and a counter electrode 3; and a plurality of reaction reagent layers that are provided on the substrate 1 and include pyrophosphatase, glyceraldehyde-3-phosphate dehydrogenase, diaphorase, glyceraldehyde-3-phosphate, oxidized nicotineamide adenine dinucleotide, an electronic mediator, a magnesium salt and a buffer component, reaction reagent layer 36 including the enzyme being separated from reaction reagent layer 35 including the buffer component, and reaction reagent layer 37 including glyceraldehyde-3-phosphate being separated from the reaction reagent layer 35 including the buffer component.
摘要翻译：提供了一种焦磷酸盐传感器，其可以在使用引物延伸反应的SNP分型中测量焦磷酸盐的方法中以高灵敏度方便地检测焦磷酸盐。焦磷酸盐传感器，其特征在于包括：绝缘基板1; 形成在其上并具有测量电极2和对电极3的电极系统; 以及设置在基板1上的多个反应试剂层，包括焦磷酸酶，甘油醛-3-磷酸脱氢酶，心肌黄酶，甘油醛-3-磷酸，氧化尼古丁胺腺嘌呤二核苷酸，电子介体，镁盐和缓冲成分，将包含缓冲成分的反应试剂层35分离出的酶的反应试剂层36和包含甘油醛-3-磷酸的反应试剂层37与包含缓冲成分的反应试剂层35分离。

8. 发明授权

US07396648B2 Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
标题翻译： DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物
公开(公告)号：US07396648B2
公开(公告)日：2008-07-08
申请号：US11607907
申请日：2006-12-04
申请人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
发明人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6827 , Y10T436/143333
摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is G. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of T or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.
摘要翻译：本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基一个基座间隔开的是G.根据本发明，3'端基被设计为对应于SNP的基极; 从3'端的第二个基地是T或G; 从3'的第三个基地是T或G的任何一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

9. 发明授权

US07348150B1 Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
公开(公告)号：US07348150B1
公开(公告)日：2008-03-25
申请号：US11633587
申请日：2006-12-05
申请人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
发明人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6851 , C12Q2565/301
摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is C. According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of A, T or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

10. 发明申请

US20070281341A1 Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
标题翻译： DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物
公开(公告)号：US20070281341A1
公开(公告)日：2007-12-06
申请号：US11633584
申请日：2006-12-05
申请人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
发明人： Hidenobu Yaku , Hiroaki Oka , Tetsuo Yukimasa
IPC分类号： C12P19/34
CPC分类号： C12Q1/6858 , C12Q1/6869 , C12Q2535/125
摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is C.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be any one of A, T or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.
摘要翻译：本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基其中一个基座间隔开C.根据本发明，3'端基被设计为对应于SNP的基底; 从3'端的第二个基地是T或G; 3'端的第3个碱基为A，T或C之一; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

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