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1. 发明申请

US20060121559A1 Saccharide composition purification instrument 有权
公开(公告)号：US20060121559A1
公开(公告)日：2006-06-08
申请号：US11061536
申请日：2005-02-18
申请人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12P21/06 , C12M1/34 , B01D15/08
CPC分类号： C12P19/00 , B01J20/3242 , B01J2220/603 , C12N11/00 , C12P21/005 , C12P21/06
摘要： The present invention provides a sugar chain purification instrument which can continuously and efficiently purify the sugar chain. According to the present invention, the instrument includes an immobilized protease column in which a carrier which can be packed in the column and on which protease is immobilized is packed and an immobilized glycopeptidase column in which a carrier which can be packed in the column and on which glycopeptidase is immobilized is packed, these columns being connected in series, and the reaction is carried out while continuously passing a glycoprotein to be decomposed together with a suitable buffer solution through the above columns. The step of liberation of sugar chain from the glycoprotein is highly simplified and labor-saving, and liberation of the sugar chain can be moderately and simply performed.

2. 发明授权

US07556962B2 Saccharide composition purification instrument 有权
标题翻译：糖组成净化仪
公开(公告)号：US07556962B2
公开(公告)日：2009-07-07
申请号：US11061536
申请日：2005-02-18
申请人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12M1/14 , C12M3/04
CPC分类号： C12P19/00 , B01J20/3242 , B01J2220/603 , C12N11/00 , C12P21/005 , C12P21/06
摘要： The present invention provides a sugar chain purification instrument which can continuously and efficiently purify the sugar chain. According to the present invention, the instrument includes an immobilized protease column in which a carrier which can be packed in the column and on which protease is immobilized is packed and an immobilized glycopeptidase column in which a carrier which can be packed in the column and on which glycopeptidase is immobilized is packed, these columns being connected in series, and the reaction is carried out while continuously passing a glycoprotein to be decomposed together with a suitable buffer solution through the above columns. The step of liberation of sugar chain from the glycoprotein is highly simplified and labor-saving, and liberation of the sugar chain can be moderately and simply performed.
摘要翻译：本发明提供一种可连续高效地纯化糖链的糖链纯化装置。根据本发明，仪器包括固定化的蛋白酶柱，其中可以填充在柱中并且固定有蛋白酶的载体和固定的糖肽酶柱，其中可以包装在柱中的载体和将这些糖肽酶固定化，将这些柱串联连接，在连续通过糖蛋白的同时进行反应，并通过上述柱与合适的缓冲溶液一起分解。从糖蛋白释放糖链的步骤被高度简化和省力，可以适度简单地进行糖链的释放。

3. 发明授权

US07883873B2 Saccharide composition synthesizer 有权
标题翻译：糖组成合成仪
公开(公告)号：US07883873B2
公开(公告)日：2011-02-08
申请号：US11060810
申请日：2005-02-18
申请人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12P19/18 , C12P19/16 , B01J19/00
CPC分类号： C12P19/18
摘要： The present invention provides a sugar chain synthesizer capable of continuously reacting sugar chains when a plurality of sugar chains are successively reacted. The sugar chain synthesizer of the present invention includes a plurality of vessels containing respective sugar nucleotide solutions, a plurality of vessels containing respective glycosyltransferases, and a reactor containing a primer that is a water-soluble polymer, into which the above described sugar nucleotide solution and glycosyltransferase are introduced. In the present invention, components in a reaction solution obtained in the reactor are separated through an ultrafiltration column, and a reaction product is then returned to the above described reactor, so as to continuously synthesize sugar chains. Although it is a complicated synthesis of sugar chains, it becomes possible to carry out such synthesis continuously and automatically.
摘要翻译：本发明提供了当多个糖链连续反应时能够连续反应糖链的糖链合成器。本发明的糖链合成装置包括含有各糖苷溶液的多个容器，含有各糖基转移酶的多个容器，以及含有水溶性聚合物的底物的反应器，上述糖核苷酸溶液和糖基转移酶。在本发明中，通过超滤塔将反应器中得到的反应溶液中的成分分离，然后将反应产物返回到上述反应器中，以连续合成糖链。糖链的合成虽然复杂，但可以连续自动进行合成。

4. 发明申请

US20050221472A1 Saccharide composition synthesizer 有权
标题翻译：糖组成合成仪
公开(公告)号：US20050221472A1
公开(公告)日：2005-10-06
申请号：US11060810
申请日：2005-02-18
申请人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Masanori Takaki , Kuriko Yamada , Kisaburo Deguchi , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12P19/18 , B01J8/04 , C12M1/34 , C12M1/40
CPC分类号： C12P19/18
摘要： The present invention provides a sugar chain synthesizer capable of continuously reacting sugar chains when a plurality of sugar chains are successively reacted. The sugar chain synthesizer of the present invention includes a plurality of vessels containing respective sugar nucleotide solutions, a plurality of vessels containing respective glycosyltransferases, and a reactor containing a primer that is a water-soluble polymer, into which the above described sugar nucleotide solution and glycosyltransferase are introduced. In the present invention, components in a reaction solution obtained in the reactor are separated through an ultrafiltration column, and a reaction product is then returned to the above described reactor, so as to continuously synthesize sugar chains. Although it is a complicated synthesis of sugar chains, it becomes possible to carry out such synthesis continuously and automatically.
摘要翻译：本发明提供了当多个糖链连续反应时能够连续反应糖链的糖链合成器。本发明的糖链合成装置包括含有各糖苷溶液的多个容器，含有各糖基转移酶的多个容器，以及含有水溶性聚合物的底物的反应器，上述糖核苷酸溶液和糖基转移酶。在本发明中，通过超滤塔将反应器中得到的反应溶液中的成分分离，然后将反应产物返回到上述反应器，以连续合成糖链。糖链的合成虽然复杂，但可以连续自动进行合成。

5. 发明授权

US07163379B2 Gradient liquid feed pump system, and liquid chromatograph 有权
标题翻译：梯度液体进料泵系统和液相色谱仪
公开(公告)号：US07163379B2
公开(公告)日：2007-01-16
申请号：US10504880
申请日：2003-03-17
申请人： Kiyotoshi Mori , Kenichiro Takahashi , Masanori Takaki , Masato Fukuda , Masahito Ito , Hironori Kaji
发明人： Kiyotoshi Mori , Kenichiro Takahashi , Masanori Takaki , Masato Fukuda , Masahito Ito , Hironori Kaji
IPC分类号： F04B41/06
CPC分类号： F04B11/0075 , F04B13/02 , F04B2201/1201 , F04B2201/1208 , F04B2205/05 , G01N30/32 , G01N30/34 , G01N2030/326
摘要： A gradient liquid feed pump system capable of liquid feed in a correct mixing ratio even in the case of liquid feed in which the mixing ratio varies. Specifically, it comprises a first plunger pump for sucking and delivering an eluate, a second the first and second plunger pumps through a cam, a first pressure sensor for measuring the pressure in the flow path of the second plunger pump, a second pressure sensor for measuring the pressure in the fist plunger pump, a cam position detecting sensor for detecting the cam position, wherein when the cam position is in a delivery interval, the rotary speed of the motor is increased, and the period for decreasing the rotary speed is determined on the basis of the difference between the pressure values obtained from the first and second pressure sensors, respectively. Thereby, liquid feed can be smoothly effected with correct mixing ratio while avoiding pressure interference between pumps.
摘要翻译：即使在混合比变化的液体进料的情况下，也能以正确的混合比液体进料的梯度液体供给泵系统。具体地，它包括用于吸入和输送洗脱液的第一柱塞泵，通过凸轮的第一和第二柱塞泵，用于测量第二柱塞泵的流动路径中的压力的第一压力传感器，用于测量第二柱塞泵的流路中的压力的第二压力传感器，测量第一柱塞泵中的压力，用于检测凸轮位置的凸轮位置检测传感器，其中当凸轮位置处于输送间隔时，马达的转速增加，并且确定用于降低转速的周期基于从第一和第二压力传感器获得的压力值之间的差异。由此，能够以正确的混合比平稳地进行液体供给，同时避免泵之间的压力干扰。

6. 发明申请

US20050084386A1 Gradient liquid feed pump system, and liquid chromatograph 有权
标题翻译：梯度液体进料泵系统和液相色谱仪
公开(公告)号：US20050084386A1
公开(公告)日：2005-04-21
申请号：US10504880
申请日：2003-03-17
申请人： Kiyotoshi Mori , Kenichiro Takahashi , Masanori Takaki , Masato Fukuda , Masahito Ito , Hironori Kaji
发明人： Kiyotoshi Mori , Kenichiro Takahashi , Masanori Takaki , Masato Fukuda , Masahito Ito , Hironori Kaji
IPC分类号： F04B11/00 , F04B13/02 , G01N30/32 , G01N30/34 , F04B23/08 , F04B3/00
CPC分类号： F04B11/0075 , F04B13/02 , F04B2201/1201 , F04B2201/1208 , F04B2205/05 , G01N30/32 , G01N30/34 , G01N2030/326
摘要： A gradient liquid feed pump system capable of liquid feed in a correct mixing ratio even in the case of liquid feed in which the mixing ratio varies. Specifically, it comprises a first plunger pump for sucking and delivering an eluate, a second the first and second plunger pumps through a cam, a first pressure sensor for measuring the pressure in the flow path of the second plunger pump, a second pressure sensor for measuring the pressure in the fist plunger pump, a cam position detecting sensor for detecting the cam position, wherein when the cam position is in a delivery interval, the rotary speed of the motor is increased, and the period for decreasing the rotary speed is determined on the basis of the difference between the pressure values obtained from the first and second pressure sensors, respectively. Thereby, liquid feed can be smoothly effected with correct mixing ratio while avoiding pressure interference between pumps.
摘要翻译：即使在混合比变化的液体进料的情况下，也能以正确的混合比液体进料的梯度液体供给泵系统。具体地，它包括用于吸入和输送洗脱液的第一柱塞泵，通过凸轮的第一和第二柱塞泵，用于测量第二柱塞泵的流动路径中的压力的第一压力传感器，用于测量第二柱塞泵的流路中的压力的第二压力传感器，测量第一柱塞泵中的压力，用于检测凸轮位置的凸轮位置检测传感器，其中当凸轮位置处于输送间隔时，马达的转速增加，并且确定用于降低转速的周期基于从第一和第二压力传感器获得的压力值之间的差异。由此，能够以正确的混合比平稳地进行液体供给，同时避免泵之间的压力干扰。

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