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1. 发明申请

US20130145828A1 COLUMN PACKING FOR LIQUID CHROMATOGRAPHY, SEPARATION COLUMN, AND LIQUID CHROMATOGRAPHY DEVICE 审中-公开
标题翻译：用于液相色谱柱，分离柱和液相色谱装置的包装
公开(公告)号：US20130145828A1
公开(公告)日：2013-06-13
申请号：US13818035
申请日：2011-08-26
申请人： Masako Kawarai , Masahito Ito , Norimasa Minamoto
发明人： Masako Kawarai , Masahito Ito , Norimasa Minamoto
IPC分类号： B01J39/26 , G01N30/50
CPC分类号： B01J39/26 , B01J20/286 , B01J20/321 , B01J20/327 , B01J20/328 , B01J20/3282 , B01J20/3293 , B01J39/20 , B01J2220/54 , G01N30/50 , G01N2030/525 , G01N2030/567
摘要： To provide a column packing, a separation column, an analysis device, and an analysis method to reduce the analysis time without deteriorating the separation of multi-component amino acids. Disclosed are a column packing comprising a core portion made of a styrene-di-vinylbenzene copolymer having a cross-linkage degree of 50% or more and a shell portion made of a styrene-di-vinylbenzene copolymer having a cross-linkage degree of 20% or less; a separation column using the column packing; and a high-speed liquid chromatography analysis device and a analysis method using the separation column.
摘要翻译：提供柱填充，分离柱，分析装置和分析方法，以减少分析时间，而不会降低多组分氨基酸的分离。公开了一种柱填料，其包含由交联度为50％以上的苯乙烯 - 二乙烯基苯共聚物制成的核心部分和由交联度为20的苯乙烯 - 二乙烯基苯共聚物制成的壳部分％或更少; 使用柱填料的分离柱; 和高速液相色谱分析装置以及使用分离塔的分析方法。

2. 发明授权

US08232079B2 Oligosaccharide synthesizer 失效
标题翻译：寡糖合成仪
公开(公告)号：US08232079B2
公开(公告)日：2012-07-31
申请号：US11197335
申请日：2005-08-05
申请人： Kisaburo Deguchi , Genzou Hirata , Masahito Ito , Hiroaki Nakagawa , Shinichiro Nishimura
发明人： Kisaburo Deguchi , Genzou Hirata , Masahito Ito , Hiroaki Nakagawa , Shinichiro Nishimura
IPC分类号： C12M1/00 , C12P19/18
CPC分类号： C12P19/30
摘要： After various sugar nucleotide solutions and glycosyltransferases (or primers) have been mixed, they are introduced into a reaction tank (column) with primers (or glycosyltransferases) immobilized thereon. Then solutions coming out of the reaction tank are led to an ultrafiltration column. The oligosaccharide synthesizer according to the present invention is equipped with a flow path for ensuring that glycosyltransferases or primers separated by the ultrafiltration column are returned into a container for storing each solution in a sample injector.
摘要翻译：在各种糖核苷酸溶液和糖基转移酶（或引物）已被混合之后，将它们引入固定在其上的引物（或糖基转移酶）的反应罐（柱）中。然后将从反应槽出来的溶液引入超滤柱。根据本发明的寡糖合成仪配备有用于确保由超滤塔分离的糖基转移酶或引物返回到用于将每种溶液存储在样品注射器中的容器中的流动路径。

3. 发明申请

US20120058568A1 LIQUID CHROMATOGRAPH AND ANALYSIS METHOD 审中-公开
标题翻译：液相色谱和分析方法
公开(公告)号：US20120058568A1
公开(公告)日：2012-03-08
申请号：US13202710
申请日：2010-03-03
申请人： Midori Sasaki , Masako Ishikawa , Hiroaki Nakagawa , Hiroshi Suzuki , Masahito Ito
发明人： Midori Sasaki , Masako Ishikawa , Hiroaki Nakagawa , Hiroshi Suzuki , Masahito Ito
IPC分类号： G01N30/02 , G01N21/64
CPC分类号： G01N30/84 , G01N30/32 , G01N30/74 , G01N2030/8435
摘要： It is an object of the present invention to provide a liquid chromatograph which, in a high performance liquid chromatograph analysis using a post-column method, can maintain mixing precision of a sample eluted from a column and a reaction reagent without a special mixing and reacting portion, prevent diffusion of a target component, and perform measurement with high sensitivity.The liquid chromatograph of the present invention has a mobile phase feed pump 1 with an allowable pressure higher than 40 MPa, a separation column 3 having a packing material with a particle diameter of 3 μm or smaller, and a reaction reagent feed pump 8 to which a pressurizing coil 9 for increasing the pressure acting on the reaction reagent feed pump 8 is connected.
摘要翻译：本发明的目的是提供一种液相色谱仪，其在使用柱后法的高效液相色谱分析中可以保持从柱和反应试剂中洗脱的样品的混合精度，而无需特别的混合和反应防止目标成分的扩散，高灵敏度地进行测定。本发明的液相色谱仪具有容许压力高于40MPa的流动相进料泵1，具有粒径为3μm以下的填充材料的分离塔3和反应试剂供给泵8，连接用于增加作用在反应试剂供给泵8上的压力的加压线圈9。

4. 发明申请

US20110097813A1 THREE-DIMENSIONAL LIQUID CHROMATOGRAPHY 审中-公开
标题翻译：三维液相色谱
公开(公告)号：US20110097813A1
公开(公告)日：2011-04-28
申请号：US12917084
申请日：2010-11-01
申请人： Masahito ITO , Junkichi MIURA , Kisaburo DEGUCHI
发明人： Masahito ITO , Junkichi MIURA , Kisaburo DEGUCHI
IPC分类号： G01N30/02 , B01D15/08 , B01D15/04
CPC分类号： G01N30/463 , G01N30/461 , G01N30/7233 , G01N30/96 , B01D15/322 , B01D15/362 , B01D15/325 , B01D15/363
摘要： In a liquid chromatography apparatus, a separation column of intermediate stage is additionally connected between a separation column of first stage and a separation column of second stage. Preferably, a switching unit and a liquid feed unit for mixing and feeding a plurality of solutions are added to improve a separation capability. A three-dimensional liquid chromatography apparatus capable of avoiding the “solution interference” can be realized. Even a complex sample containing a hydrophilic component and a hydrophobic component in a mixed state can be separated and analyzed satisfactorily on-line.
摘要翻译：在液相色谱装置中，中间级分离塔另外连接在第一级分离塔和第二级分离塔之间。优选地，添加用于混合和供给多个溶液的切换单元和液体进料单元以提高分离能力。可以实现能够避免“溶液干扰”的三维液相色谱仪。即使是含有亲水性成分和混合状态的疏水性成分的复合物，也可以在线进行分离和分析。

5. 发明授权

US07699990B2 Separation analyzer 失效
标题翻译：分离分析仪
公开(公告)号：US07699990B2
公开(公告)日：2010-04-20
申请号：US11581147
申请日：2006-10-16
申请人： Kisaburo Deguchi , Shinya Ito , Masahito Ito
发明人： Kisaburo Deguchi , Shinya Ito , Masahito Ito
IPC分类号： B01D15/08
CPC分类号： G01N35/1097 , G01N1/38 , G01N30/20 , G01N30/34 , G01N2030/027 , Y10T436/2575
摘要： A separation analyzer for transferring solutions, while changing mixing ratios of the solutions, wherein a flow passage from a first pump, a flow passage from a second pump, and a passage for sample introduction section, the flow passages being connected, which further comprises a flow switching means for temporarily storing the solutions, whereby the flow switching means alternatively makes a first state for pushing out the solutions in a second sampling loop to the sample introduction section by the second pump, while transferring the solutions to the first sampling loop; and a second state for pushing out the solutions in the first sampling loop by the second pump, while transferring the solutions from the first pump to the second sampling loop.
摘要翻译：一种用于输送溶液的分离器，同时改变溶液的混合比，其中来自第一泵的流动通道，来自第二泵的流动通道和用于样品引入部分的通道，流动通道连接，还包括流动切换装置，用于临时存储溶液，由此流动切换装置交替地将第二状态用于将第二取样回路中的溶液推出到第二泵的样品引入部分，同时将溶液转移到第一取样回路; 以及第二状态，用于在将所述解决方案从所述第一泵传送到所述第二采样回路的同时，通过所述第二泵推出所述第一采样回路中的所述解。

6. 发明申请

US20090090173A1 LIQUID CHROMATOGRAPHY APPARATUS AND LIQUID CHROMATOGRAPHY ANALYSIS METHOD 失效
标题翻译：液相色谱仪和液相色谱分析方法
公开(公告)号：US20090090173A1
公开(公告)日：2009-04-09
申请号：US12244846
申请日：2008-10-03
申请人： Masato Fukuda , Hiroyuki Wada , Masahito Ito , Hironori Kaji , Kosaku Toyosaki
发明人： Masato Fukuda , Hiroyuki Wada , Masahito Ito , Hironori Kaji , Kosaku Toyosaki
IPC分类号： G01N30/04
CPC分类号： G01N30/34
摘要： An object of the present invention is to improve the accuracy in analysis repeatability of a liquid chromatography apparatus that feeds different eluent by use of a plurality of pumps and achieves the mixture of eluent.Each of the pumps includes means for notifying an automatic sampler and a higher-level control unit that the specified timing of a liquid feeding cycle is reached. A pump whose liquid feeding cycle is the slowest transmits own information so that analysis is synchronized with a liquid feeding cycle. Moreover, a cycle position of a liquid feeding cycle at the end of analysis is expected, and the analysis time is automatically adjusted so that the wait time until the start of the next analysis becomes the shortest.
摘要翻译：本发明的目的是提高通过使用多个泵进料不同洗脱液的液相色谱仪的分析重复性的准确性，并实现洗脱液的混合。每个泵包括用于通知自动取样器和上位控制单元的装置，以达到给定循环的指定时间。送液周期最慢的泵传送自己的信息，使分析与液体供给循环同步。此外，期望在分析结束时的送液循环的循环位置，并且自动调整分析时间，使得直到下一次分析开始的等待时间变得最短。

7. 发明申请

US20090062151A1 COMPUTERIZED METHOD AND APPARATUS FOR ANALYZING AMINO ACIDS 失效
标题翻译：用于分析氨基酸的计算方法和装置
公开(公告)号：US20090062151A1
公开(公告)日：2009-03-05
申请号：US12196693
申请日：2008-08-22
申请人： Yoshio Fujii , Masahito Ito , Kimiyoshi Koda
发明人： Yoshio Fujii , Masahito Ito , Kimiyoshi Koda
IPC分类号： C40B60/12
CPC分类号： G01N33/6806 , G01N30/34 , G01N30/8631 , G01N2030/8804 , G01N2030/8813 , Y10T436/2575
摘要： A method for analyzing a plurality of amino acids in a fluid sample by a user is provided comprising the steps of introducing the sample into a buffer solution, passing the sample in the buffer solution through a separation column and setting a lithium ion concentration in the buffer to no more than 0.3 mols/L up to a time before β-aminoisobutyric acid (β-AiBA) is eluted.
摘要翻译：提供了一种用户分析流体样品中的多种氨基酸的方法，包括以下步骤：将样品引入缓冲溶液中，使样品在缓冲溶液中通过分离柱，并将缓冲液中的锂离子浓度设定在缓冲液在β-氨基异丁酸（β-AiBA）洗脱之前至多不超过0.3摩尔/升。

8. 发明授权

US07432108B2 Computerized method and apparatus for analyzing amino acids 失效
标题翻译：用于分析氨基酸的计算机化方法和装置
公开(公告)号：US07432108B2
公开(公告)日：2008-10-07
申请号：US11355988
申请日：2006-02-17
申请人： Yoshio Fujii , Masahito Ito , Kimiyoshi Koda
发明人： Yoshio Fujii , Masahito Ito , Kimiyoshi Koda
IPC分类号： G01N33/00
CPC分类号： G01N33/6806 , G01N30/34 , G01N30/8631 , G01N2030/8804 , G01N2030/8813 , Y10T436/2575
摘要： A method for analyzing a plurality of amino acids in a fluid sample by a user is provided comprising the steps of introducing the sample into a buffer solution, passing the sample in the buffer solution through a separation column and setting a lithium ion concentration in the buffer to no more than 0.3 mols/L up to a time before β-aminoisobutyric acid (β-AiBA) is eluted.
摘要翻译：提供了一种用户分析流体样品中的多种氨基酸的方法，包括以下步骤：将样品引入缓冲溶液中，使样品在缓冲溶液中通过分离柱，并将缓冲液中的锂离子浓度设定在缓冲液在β-氨基异丁酸（β-AiBA）洗脱之前至多不超过0.3摩尔/升。

9. 发明申请

US20080172186A1 Method and apparatus for analyzing multi-channel chromatogram 失效
标题翻译：用于分析多通道色谱图的方法和装置
公开(公告)号：US20080172186A1
公开(公告)日：2008-07-17
申请号：US12073964
申请日：2008-03-12
申请人： Masahito Ito , Kisaburo Deguchi
发明人： Masahito Ito , Kisaburo Deguchi
IPC分类号： G01N31/00 , G06F19/00
CPC分类号： G01N30/8624 , G01N30/8631 , G01N30/8679 , G01N2201/1293
摘要： A method for analyzing a multi-channel chromatogram is realized for accurately resolving overlapping peaks on a multi-channel chromatogram to permit analysis of the composition of a sample. First, components of overlapping peaks are specified. A data matrix Dij is determined, and compressed in the wavelength direction to obtain a data matrix vector Di. Next, the two-dimensional data is deconvoluted, final standard deviation s0 is registered, and the deconvoluted two-dimensional data is reconvoluted to a retention intensity matrix. A quantitative spectral intensity matrix is computed, components are identified, and the identified components are quantified. Subsequently, an eigenvalue problem is solved, and the number n of components is estimated. The deconvolution and computation of the eigenvalue problem are iteratively executed until the peaks are isolated. Then, elution profiles are calculated, the components are identified, and the identified components are quantified.
摘要翻译：实现了分析多通道色谱图的方法，用于准确地分辨多通道色谱图上的重叠峰，以便分析样品的组成。首先，指定重叠峰的分量。确定数据矩阵Dij，并在波长方向上压缩以获得数据矩阵向量Di。接下来，对二维数据进行解卷积，记录最终标准偏差s 0，并将去卷积的二维数据重新卷积到保留强度矩阵。计算定量光谱强度矩阵，识别组件，并对所识别的组分进行量化。随后，解决了特征值问题，并且估计了分量的数量n。迭代执行反褶积和特征值问题的计算，直到峰被隔离。然后，计算洗脱曲线，识别组分，并对所鉴定的组分进行定量。

10. 发明申请

US20070219199A1 Use of Sulfonanilides as Agricultural and Horticultural Fungicide 有权
标题翻译：磺酰苯胺作为农业和园艺杀菌剂的使用
公开(公告)号：US20070219199A1
公开(公告)日：2007-09-20
申请号：US11632832
申请日：2005-07-21
申请人： Koichi Araki , Sachio Kudo , Yoshitaka Sato , Masahito Ito , Takuya Gomibuchi , Yasuo Araki , Tetsuya Inuta , Keiji Endo , Shinichi Shirakura , Shin Nakamura
发明人： Koichi Araki , Sachio Kudo , Yoshitaka Sato , Masahito Ito , Takuya Gomibuchi , Yasuo Araki , Tetsuya Inuta , Keiji Endo , Shinichi Shirakura , Shin Nakamura
IPC分类号： A01N43/66 , A01N43/54 , C07D239/00 , C07D251/00
CPC分类号： A01N43/54 , A01N43/66 , C07D239/24 , C07D239/52 , C07D251/12 , C07D251/20
摘要： Compounds of the formula (I), wherein Z represents C—R7 or N, and R represents alkyl that may be optionally substituted, alkenyl, alkynyl, cycloalkyl or alkoxycarbonylamino, are useful as agricultural and horticultural fungicides.
摘要翻译：式（I）化合物，其中Z表示C-R 7或N，R表示可任选取代的烷基，烯基，炔基，环烷基或烷氧基羰基氨基，可用作农业和园艺杀真菌剂。

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