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1. 发明申请

US20100015672A1 Gene Expression Cassette and a Transformant, and a Method for Manufacturing 2-Deoxy-Scyllo-Inosose and a Method for Purifying 2-Deoxy-Scyllo-Inosose Using Said Transformant 有权
标题翻译：基因表达盒和转化体及其制备方法，以及使用所述转化体纯化2-脱氧肌氨酸的方法
公开(公告)号：US20100015672A1
公开(公告)日：2010-01-21
申请号：US11887445
申请日：2006-03-23
申请人： Masamichi Takagi , Takahisa Kogure , Naoki Wakisaka , Hiroaki Takaku , Katsumi Ajisaka , Tatsuo Miyazaki , Masao Hirayama
发明人： Masamichi Takagi , Takahisa Kogure , Naoki Wakisaka , Hiroaki Takaku , Katsumi Ajisaka , Tatsuo Miyazaki , Masao Hirayama
IPC分类号： C12P19/02 , C12N15/00 , C12N1/21 , C07H3/02
CPC分类号： C12P17/06 , C07D309/30 , C12N9/88 , C12N9/90 , C12N9/92 , C12P7/26 , C12Y503/01009
摘要： A transformant is prepared to insert at least a gene expression cassette comprising a gene involved in the synthesis of 2-deoxy-scyllo-inosose into E. coli as host cells. A 2-deoxy-scyllo-inosose is synthesized from D-glucose, oligosaccharide, polysaccharide, starch and rice bran, using the transformant. A culture solution containing the 2-deoxy-scyllo-inosose is treated with a mixed bed or double bed type column comprising a hydrogen form of strong acidic cation exchange resin and an organic ion form of basic anion exchange resin. The 2-deoxy-scyllo-inosose as purified is reacted with trimethoxymethane to convert into 2-deoxy-scyllo-inosose dimethylketal, and the dimethylketal is crystallized and purified. Then, DOI is highly purified through hydrolyzing the dimethylketal in the presence of acid.
摘要翻译：制备转化体至少将含有参与合成2-脱氧 - 青蟹肌糖的基因的基因表达盒插入大肠杆菌作为宿主细胞。使用转化体从D-葡萄糖，寡糖，多糖，淀粉和米糠合成2-脱氧 - 青蟹肌糖。含有2-脱氧 - 青蟹肌糖的培养液用含氢形式的强酸性阳离子交换树脂和有机离子形式的碱性阴离子交换树脂的混合床或双床型柱处理。将纯化的2-脱氧 - 青蟹肌糖与三甲氧基甲烷反应以转化成2-脱氧 - 鲨糖醛糖二甲基缩酮，并将二甲基缩酮结晶并纯化。然后，DOI通过在酸存在下水解二甲基缩酮而高度纯化。

2. 发明授权

US08758741B2 Gene expression cassette and a transformant, and a method for manufacturing 2-deoxy-scyllo-inosose and a method for purifying 2-deoxy-scyllo-inosose using said transformant 有权
标题翻译：基因表达盒和转化体，以及2-脱氧十字鸟苷的制造方法以及使用所述转化体纯化2-脱氧十字鸟苷的方法
公开(公告)号：US08758741B2
公开(公告)日：2014-06-24
申请号：US11887445
申请日：2006-03-23
申请人： Masamichi Takagi , Takahisa Kogure , Naoki Wakisaka , Hiroaki Takaku , Katsumi Ajisaka , Tatsuo Miyazaki , Masao Hirayama
发明人： Masamichi Takagi , Takahisa Kogure , Naoki Wakisaka , Hiroaki Takaku , Katsumi Ajisaka , Tatsuo Miyazaki , Masao Hirayama
IPC分类号： A01N63/00 , C12N15/09 , C12N15/63 , C07H21/04
CPC分类号： C12P17/06 , C07D309/30 , C12N9/88 , C12N9/90 , C12N9/92 , C12P7/26 , C12Y503/01009
摘要： A transformant is prepared to insert at least a gene expression cassette comprising a gene involved in the synthesis of 2-deoxy-scyllo-inosose into E. coli as host cells. A 2-deoxy-scyllo-inosose is synthesized from D-glucose, oligosaccharide, polysaccharide, starch and rice bran, using the transformant. A culture solution containing the 2-deoxy-scyllo-inosose is treated with a mixed bed or double bed type column comprising a hydrogen form of strong acidic cation exchange resin and an organic ion form of basic anion exchange resin. The 2-deoxy-scyllo-inosose as purified is reacted with trimethoxymethane to convert into 2-deoxy-scyllo-inosose dimethylketal, and the dimethylketal is crystallized and purified. Then, DOI is highly purified through hydrolyzing the dimethylketal in the presence of acid.
摘要翻译：制备转化体至少将含有参与合成2-脱氧 - 青蟹肌糖的基因的基因表达盒插入大肠杆菌作为宿主细胞。使用转化体从D-葡萄糖，寡糖，多糖，淀粉和米糠合成2-脱氧 - 青蟹肌糖。含有2-脱氧 - 青蟹肌糖的培养液用含氢形式的强酸性阳离子交换树脂和有机离子形式的碱性阴离子交换树脂的混合床或双床型柱处理。将纯化的2-脱氧 - 青蟹肌糖与三甲氧基甲烷反应以转化成2-脱氧 - 鲨糖醛糖二甲基缩酮，并将二甲基缩酮结晶并纯化。然后，DOI通过在酸存在下水解二甲基缩酮而高度纯化。

3. 发明授权

US09243071B2 Fusion protein for suppression of autoantibodies 有权
标题翻译：用于抑制自身抗体的融合蛋白
公开(公告)号：US09243071B2
公开(公告)日：2016-01-26
申请号：US14110287
申请日：2012-04-02
申请人： Masayuki Homma , Takahisa Kogure , Kenji Nakajima
发明人： Masayuki Homma , Takahisa Kogure , Kenji Nakajima
IPC分类号： A61K39/00 , C12N15/62 , C12N15/63 , C07K16/46 , C07K16/42 , C07K14/705 , A61K38/00
CPC分类号： C07K16/42 , A61K38/00 , C07K14/705 , C07K2319/00 , C07K2319/30
摘要： A new fusion protein which can specifically suppress the autoantibodies, which can effectively prevent or treat the autoimmune disease of autoantibody type, and which can be expressed in an amount sufficient for industrial production. A fusion protein, characterized in that, a protein (X) containing a site recognized by autoantibodies which are a cause of the autoimmune disease of autoantibody type is connected to a protein (A) containing a fragment of the antibody heavy chain constant region which exhibits the antibody-dependent cellular cytotoxicity with a linker peptide (L) consisting of one or more amino acid(s), wherein the protein (X), the linker peptide (L) and the protein (A) are connected in this order by means of peptide bond from N terminal to C terminal.
摘要翻译：一种可以特异性抑制自身抗体的新的融合蛋白，其可以有效预防或治疗自身抗体型的自身免疫性疾病，并且其表达量足以用于工业生产。一种融合蛋白，其特征在于，含有自身抗体自身抗体的自身抗体识别部位的蛋白质（X）与包含抗体重链恒定区片段的蛋白质（A）连接，所述蛋白质与由一个或多个氨基酸组成的接头肽（L）的抗体依赖性细胞毒性，其中所述蛋白质（X），接头肽（L）和蛋白质（A）以该顺序依次连接的肽键从N端到C端。

4. 发明申请

US20140335085A1 FUSION PROTEIN 有权
标题翻译：融合蛋白
公开(公告)号：US20140335085A1
公开(公告)日：2014-11-13
申请号：US14110287
申请日：2012-04-02
申请人： Masayuki Homma , Takahisa Kogure , Kenji Nakajima
发明人： Masayuki Homma , Takahisa Kogure , Kenji Nakajima
IPC分类号： C07K16/42 , C07K14/705
CPC分类号： C07K16/42 , A61K38/00 , C07K14/705 , C07K2319/00 , C07K2319/30
摘要： A new fusion protein which can specifically suppress the autoantibodies, which can effectively prevent or treat the autoimmune disease of autoantibody type, and which can be expressed in an amount sufficient for industrial production. A fusion protein, characterized in that, a protein (X) containing a site recognized by autoantibodies which are a cause of the autoimmune disease of autoantibody type is connected to a protein (A) containing a fragment of the antibody heavy chain constant region which exhibits the antibody-dependent cellular cytotoxicity with a linker peptide (L) consisting of one or more amino acid(s), wherein the protein (X), the linker peptide (L) and the protein (A) are connected in this order by means of peptide bond from N terminal to C terminal.
摘要翻译：一种可以特异性抑制自身抗体的新的融合蛋白，其可以有效预防或治疗自身抗体型的自身免疫性疾病，并且其表达量足以用于工业生产。一种融合蛋白，其特征在于，含有自身抗体自身抗体的自身抗体识别部位的蛋白质（X）与包含抗体重链恒定区片段的蛋白质（A）连接，所述蛋白质与由一个或多个氨基酸组成的接头肽（L）的抗体依赖性细胞毒性，其中所述蛋白质（X），接头肽（L）和蛋白质（A）以该顺序依次连接的肽键从N端到C端。

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