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1. 发明申请

WO9930130A3 METHOD FOR INVESTIGATING OR STRUCTURING A SURFACE LAYER 审中-公开
标题翻译：程序对表面层或结构
公开(公告)号：WO9930130A3
公开(公告)日：2002-08-29
申请号：PCT/EP9807597
申请日：1998-11-25
申请人： MICRONAS GMBH , WOLF BERNHARD , GAHLE HANS-JUERGEN , IGEL GUENTER , BAUMANN WERNER , EHRET RALF , LEHMANN MIRKO
发明人： WOLF BERNHARD , GAHLE HANS-JUERGEN , IGEL GUENTER , BAUMANN WERNER , EHRET RALF , LEHMANN MIRKO
IPC分类号： G01N33/543 , G01B11/30 , G01N13/10 , G01N21/17 , G01N21/45 , G01N21/84 , G01N21/94 , G01N27/414 , G01N27/416 , H01L21/66 , G01N13/00 , G01N21/75
CPC分类号： H01L22/24 , G01B11/30 , G01N21/94
摘要： The invention relates to a method for investigating the surface of an object for a topographical and/or chemical characteristic. According to said method, the surface of the object is subjected to the action of surface structure selective (for investigating a topographical characteristic) and/or chemoselective (for investigating a chemical characteristic) biocomponents and a nutrient- or osmotic protective medium in such a way that the biocomponents in the nutrient- or osmotic protective medium are in contact with the object surface or are at a distance from the object surface which is within their range of detection. The object surface is then investigated using the biocomponents in the nutrient- or osmotic protective medium and at least one investigation measuring value is determined. The investigation measuring value is then compared to a reference measuring value and the result of this comparison is used to draw conclusions as to the topographical and/or chemical characteristics of the object. The invention provides a simple, very precise method for investigating the surface of an object for a topographical and/or chemical characteristic.
摘要翻译：在用于检查与表面结构选择性和/或用于与化学选择性的生物成份，并用营养或渗透保护介质中的化学性质的检查地形特性的检查对象的表面上的物体表面的地形和/或化学属性，这是这样一种方法施加是在营养或渗透保护介质与生物组分相接触布置在物体表面或小于所述物体表面的生物成份的检测范围间隔开。接着，将对象表面与布置在营养物或渗透保护介质的生物成份通过确定至少一个测量值的调查研究。然后，研究测量值与一个基准测量值，并从比较的结果推导出所述物体的地形和/或化学性质。用方法，对象表面可以很容易地以高灵敏度分析，以地形和/或化学特性。

2. 发明申请

WO0071669A8 DEVICE FOR PERFORMING ANALYSES ON CELL-CULTURES 审中-公开
标题翻译：设备技术开展研究对细胞培养物
公开(公告)号：WO0071669A8
公开(公告)日：2001-02-22
申请号：PCT/EP0003860
申请日：2000-04-28
申请人： MICRONAS GMBH , LEHMANN MIRKO , BRISCHWEIN MARTIN , BAUMANN WERNER , EHRET RALF , FREUND INGO , WOLF BERNHARD
发明人： LEHMANN MIRKO , BRISCHWEIN MARTIN , BAUMANN WERNER , EHRET RALF , FREUND INGO , WOLF BERNHARD
IPC分类号： C12M1/34 , B01L3/00 , C12M3/00 , C12M3/04
CPC分类号： C12M23/12 , B01L3/505 , C12M23/38
摘要： The invention relates to a device (1) for performing analyses on cell-cultures (2) which are present in a liquid culture medium (4). The device has at least one receptacle (3) for receiving the culture medium containing the cell-culture. One or more sensors (6) are provided for the cell-culture readings and the receptacle (3) has at least one access opening for supplying and evacuating the liquid culture medium (4). A separating element (7) can be introduced into the receptacle which is open at the top and moved into position in close proximity to the base. In this position, said separating element delimits a sub-chamber which has a reduced volume (8) in relation to the total receiving volume of the receptacle. In addition, at least one flow channel (9) is provided, which on one side adjoins the reduced-volume sub-chamber (8) and on the other adjoins a reservoir chamber (14). The sensor(s) (6) are arranged in the area of the reduced-volume sub-chamber.
摘要翻译：的装置（1）位于进行在液体培养基上延伸的细胞培养物（2）的调查（4）。该装置包括：至少一个接收器（3），用于与细胞培养物，其特征在于，一个或多个传感器提供（6），用于测量在细胞培养物中，并且其中所述接收器（3）至少一个用于供给和液体培养基的排出通路接收所述培养基（4）等具有。的分离体（7）内，所述容器在它的相对限定在插座的插座小容积室（8）的总吸收体积为地电平位置可插入到敞开的顶部容器和接合。另外，至少设置一个流动通道（9），其连接在一方面的小体积隔室（8），并且在另一方面，储液空间（149）。一个或多个传感器（6）被布置在小容积子腔室的区域中。

3. 发明专利

JP2002055102A METHOD FOR TESTING MEMBRANE ENCLOSED BIOCOMPARTMENT 失效
公开(公告)号：JP2002055102A
公开(公告)日：2002-02-20
申请号：JP2001171289
申请日：2001-06-06
申请人： MICRONAS GMBH
发明人： LEHMANN MIRKO , BAUMANN WERNER DR , MARTIN BURISSHUVAIN , RALF EHRET , HEINZ-PETER FRERIKS , FREUND INGO , GAHLE HANS-JUERGEN DR , HOFMANN KARL , MARTIN KLEINTGES , JOACHIM RUF , SIEBEN ULRICH , WOLF BERNHARD
IPC分类号： G01N33/48 , C12M1/34 , C12Q1/00 , G01N27/30 , G01N27/416 , G01N33/483
摘要： PROBLEM TO BE SOLVED: To provide a method for testing membrane enclosed biocompartments. SOLUTION: OH-ions and/or H+ions are formed from a substance existing in a culture medium and releasable and/or ingestible by the biocompartments in a culture medium region adjacently existing to the biocompartments; a first measured value of pH value of the culture medium is measured while impressing voltage; the voltage is cut off or changed so as to finish the formation of the OH-ions and the H+ions from the substance; and before or after measuring the first measured value, a second measured value of the pH value of the culture medium is measured when cutting the voltage or at a voltage of not forming hydroxy ions and hydrogen ions from the substance. After repeating this whole measurement by at least one time, the concentration of the substance in the culture medium, acidification or basification of the culture medium are decided from the first measured value and the second measured value. Thus, plural substance exchange parameters of the biocompartments can be measured in one place by this method.

4. 发明申请

WO2011116746A3 DUAL CLUTCH 审中-公开
标题翻译：双离合器
公开(公告)号：WO2011116746A3
公开(公告)日：2015-06-25
申请号：PCT/DE2011000266
申请日：2011-03-15
申请人： SCHAEFFLER TECHNOLOGIES AG , KREBS FLORIAN , NÖHL OLIVER , DAIKELER RENÉ , WOLF BERNHARD , KRAHTOV LUBEN , BOSNJAK DANIEL , AGNER IVO
发明人： KREBS FLORIAN , NÖHL OLIVER , DAIKELER RENÉ , WOLF BERNHARD , KRAHTOV LUBEN , BOSNJAK DANIEL , AGNER IVO
IPC分类号： F16D21/06 , F16D25/10
CPC分类号： F16D3/77 , F16D21/06 , F16D2021/0669
摘要： A dual clutch for coupling an engine-side input shaft (12) with a first transmission-side output shaft (28) and/or a second transmission-side output shaft (40) comprises a first clutch (18) having a first pressure plate (22) for coupling with a first clutch disk (24) connected to the first output shaft, said first pressure plate being axially mobile relative to a first counter plate (16). The dual clutch further comprises a second clutch (34) having a second pressure plate (36) for coupling with a clutch disk (38) connected to the second output shaft, said second pressure plate being axially mobile relative to a second counter plate (32). A co-rotating clutch cover (68) is connected to the first counter plate and the second counter plate. The dual clutch further comprises an actuator (48) for displacing the first pressure plate and/or the second pressure plate. According to the invention, the actuator is radially supported on the input side on the input shaft by the clutch cover and a torsional vibration damper which is indirectly or directly connected to the clutch cover, especially a flexible disk (14), and is radially mounted on the output side on one of the output shafts and/or on a transmission housing (70), thereby simplifying the design of the dual clutch, especially making a simplified absorption of the forces generated during actuation possible.
摘要翻译：对于具有第一发送侧输出轴（28）的发动机侧的输入轴（12）和/或第二传输侧输出轴（40）耦合的双离合器具有第一联接件（18），其具有相对于第一后板（16）轴向可移动的第一压力板（22），用于连接到所述第一离合器盘（24）的第一输出轴联接。此外，一个第二离合器（34）设置，其是一个相对于第二支承板（32）轴向可移动的第二压板（36），用于耦合连接到第二输出轴（38）的第二离合器板。与所述第一背板和所述第二支承板共同旋转的离合器罩（68）连接。此外，致动器（48）被提供用于移动所述第一压板和/或第二压力板，其中，所述致动装置根据在所述输入轴上的本发明通过在离合器盖的输入侧和直接地或间接地连接到离合器盖扭转振动阻尼器，特别是柔性板（14）是径向地支撑，并且被安装在输出侧的输出轴和/或变速器壳体（70）的径向中的一个。双离合器的结构简化，特别是，从致动器力产生的简化的推导是可能的。

5. 发明申请

WO9928037A8 DEVICE FOR MEASURING PHYSIOLOGICAL PARAMETERS 审中-公开
标题翻译：用于测量生理参数的装置
公开(公告)号：WO9928037A8
公开(公告)日：1999-09-02
申请号：PCT/EP9807598
申请日：1998-11-25
申请人： MICRONAS INTERMETALL GMBH , WOLF BERNHARD , SIEBEN ULRICH
发明人： WOLF BERNHARD , SIEBEN ULRICH
IPC分类号： G01N33/48 , B01L3/00 , C12M1/34 , G01N27/414 , G01N33/487 , G01N35/02
CPC分类号： G01N33/48728 , B01L3/5027 , B01L3/508 , B01L2200/025 , B01L2300/0636 , B01L2300/0645 , B01L2300/0822 , Y10S435/808
摘要： The invention relates to a device (1) for measuring physiological parameters having a testing chamber (2) which is bordered by a first semiconductor chip (3) and a second semiconductor chip (4). A seal (7) which bounds the testing chamber (2) is arranged as a spacer between said semiconductor chips. The first semiconductor chip (3) has an active side which faces the testing chamber (2) and comprises the planar sensors. Biological cells located in a nutrient medium can be adherently attached on said active side in order to directly measure physiological parameters of the cells (6). The second semiconductor chip (4) comprises at least one addition sensor situated on an active side which faces the testing chamber (2) in order to measure global physiological parameters. The semiconductor chips (3, 4) are held in a sealing position by means of a holding device (8) which externally overlaps said chips. Outside the testing chamber (2), the semiconductor chips (3, 4) each comprise electrical connection contacts (2) which contact counter-contacts of the holding device. The invention is constructed in a simple and economical manner.
摘要翻译：一种用于测量生理参数的装置（1）具有测试室（2）由第一半导体芯片（3）和第二半导体芯片（4）被该边界作为间隔物的测试室（2）密封件之间限定（7）被安排。在第一半导体芯片（3）具有（2）的面向测试室，其具有有源侧平面传感器在其上位于在营养培养基中的生物细胞（6）的贴壁anlagerbar测量生理参数直接在细胞（6）。第二半导体芯片（4）具有至少一个附加传感器，用于测量面向测试室（2）的有效侧上的全局生理参数。半导体芯片（3,4）通过与外部重叠的支架（8）保持在密封位置。在测试室（2）的外部，半导体芯片（3,4）各自具有电连接触点，所述电连接触点使保持器与配合触点接触。该设备简单且便宜。

6. 发明专利

DE19937778A1 Characterizing the functional state of living cells, especially tumor cells, comprises analyzing multiparametric data and comparing the resulting signal patterns with pre-established patterns 未知
公开(公告)号：DE19937778A1
公开(公告)日：2001-02-15
申请号：DE19937778
申请日：1999-08-10
申请人： WOLF BERNHARD
发明人： WOLF BERNHARD
IPC分类号： G01N33/50 , C12Q1/02 , C12M3/00 , C12Q3/00
摘要： Characterizing the functional state of living cells, comprising collecting data embracing several parameters of the cells, generating signal patterns based on time-series and curve analysis of the data, and subjecting the signal patterns to computer-aided comparison with physiological reaction patterns established for the cells during a learning phase, is new.

7. 发明专利

DE2938915A1 Two-chamber boiler operating system - has insulated buffer storage vessel for water above predetermined temperature 未知
公开(公告)号：DE2938915A1
公开(公告)日：1981-04-09
申请号：DE2938915
申请日：1979-09-26
申请人： WOLF BERNHARD
发明人： WOLF BERNHARD
IPC分类号： F24D3/08 , F24D11/02 , F24H1/22
摘要： The system operates a two-chamber boiler, e.g. for central heating and hot water supply in a private house, having a first fireplace for oil or gas heating and a second one for coal, coke or wood etc. When using solid fuel a common heating water circuit is used for both chambers, and the fumes pass through the other fireplace. A thermostatically-controlled pipe directs water heated above a predetermined temperature between 60 and 75 deg.C. when using solid fuel to an insulated buffer storage vessel. When heating water temp. falls below a predetermined level, heat is recovered from this vessel via a heat pump having a heat exchanger in the heating water circuit.

8. 发明专利

DE10019796A1 Adjustable-width slide-in tray for oven, comprises two parts held together in overlapping region by fastenings passed through holes 未知
公开(公告)号：DE10019796A1
公开(公告)日：2001-10-31
申请号：DE10019796
申请日：2000-04-20
申请人： WOLF BERNHARD
发明人： WOLF BERNHARD
IPC分类号： F24C15/16 , A21B3/15 , A21B3/00
摘要： Adjustable-width slide-in tray comprises two parts (2, 3) of the tray which slide together in a region (A). In the overlapping portion (B) of this region, they can be fixed together in different positions. This is achieved using fastenings which are inserted in openings (14) of the base.

9. 发明专利

CH81787A Elektrische Betätigungsvorrichtung für photographische Verschlüsse für Moment- und Zeitaufnahmen 未知
公开(公告)号：CH81787A
公开(公告)日：1919-07-16
申请号：CH81787D
申请日：1919-02-19
申请人： WOLF BERNHARD
发明人： WOLF BERNHARD

10. 发明专利

CH81622A Vorrichtung zur Betätigung eines Drahtauslösers für photographische Verschlüsse 未知
公开(公告)号：CH81622A
公开(公告)日：1919-07-01
申请号：CH81622D
申请日：1919-02-14
申请人： WOLF BERNHARD
发明人： WOLF BERNHARD

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