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1. 发明授权

US06468749B1 Sequence-dependent gene sorting techniques 失效
标题翻译：序列依赖基因分选技术
公开(公告)号：US06468749B1
公开(公告)日：2002-10-22
申请号：US09538709
申请日：2000-03-30
申请人： Levy Ulanovsky , Raja C. Mugasimangalam , Paz Einat , Dina Zezin-Sonkin , Shlomit Gilad
发明人： Levy Ulanovsky , Raja C. Mugasimangalam , Paz Einat , Dina Zezin-Sonkin , Shlomit Gilad
IPC分类号： C12Q168
CPC分类号： C12N15/66 , C12N15/10 , C12N15/1096
摘要： This invention provides a method of sorting genes comprising: (1) preparing ds cDNA molecules from mRNA molecules (2) digesting the ds cDNA molecules (3) ligating to the digested cDNA molecules a set of dsDNA oligonucleotide adaptors; (4) amplifying the ligated cDNA molecules; and (5) sorting the amplified cDNA molecules into nonoverlapping groups. This invention also provides two additional methods of sorting genes. This invention further provides a method of making sub-libraries of ligation sets.
摘要翻译：本发明提供了一种分选基因的方法，包括：（1）从mRNA分子制备ds cDNA分子（2）消化ds cDNA分子（3）连接到消化的cDNA分子一组dsDNA寡核苷酸适配子; （4）扩增连接的cDNA分子; 和（5）将扩增的cDNA分子分选成非重叠基团。本发明还提供了两种分选基因的另外的方法。本发明还提供了制备连接组的子文库的方法。

2. 发明授权

US06986988B2 Method for enrichment of natural antisense messenger RNA 失效
公开(公告)号：US06986988B2
公开(公告)日：2006-01-17
申请号：US09833031
申请日：2001-04-11
申请人： Shlomit Gilad , Paz Einat , Avital Grossman
发明人： Shlomit Gilad , Paz Einat , Avital Grossman
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N15/113 , C12N2330/10 , C12Q1/6809
摘要： A method for enrichment of natural antisense mRNA which involves hybridization of cDNA obtained from sense RNA with cDNA obtained from antisense RNA, followed by DNA polymerase treatment of the sense-antisense hybrid DNA molecule. A natural antisense library can be generated by cloning of sense-antisense hybrid DNA molecules in a vector.

3. 发明授权

US06528262B1 Method for enrichment of natural antisense messenger RNA 失效
标题翻译：富含天然反义信使RNA的方法
公开(公告)号：US06528262B1
公开(公告)日：2003-03-04
申请号：US09680420
申请日：2000-10-06
申请人： Shlomit Gilad , Paz Einat , Avital Grossman
发明人： Shlomit Gilad , Paz Einat , Avital Grossman
IPC分类号： C07H2104
CPC分类号： C12N15/113 , C12N2330/10 , C12Q1/6809
摘要： A method for enrichment of natural antisense mRNA which involves hybridization of cDNA obtained from sense RNA with cDNA obtained from antisense RNA, followed by DNA polymerase treatment of the sense-antisense hybrid DNA molecule. A natural antisense library can be generated by cloning of sense-antisense hybrid DNA molecules in a vector.
摘要翻译：富含天然反义mRNA的方法，其涉及从有义RNA获得的cDNA与从反义RNA获得的cDNA杂交，然后进行有义反义杂交DNA分子的DNA聚合酶处理。可以通过在载体中克隆正义反义杂交DNA分子来产生天然反义文库。

4. 发明申请

US20080138863A1 Methods for Cloning Nucleic Acids in a Desired Orientation 审中-公开
标题翻译：在所需方向克隆核酸的方法
公开(公告)号：US20080138863A1
公开(公告)日：2008-06-12
申请号：US10561005
申请日：2004-06-15
申请人： Paz Einat , Dina Zevin-Sonkin , Shlomit Gilad
发明人： Paz Einat , Dina Zevin-Sonkin , Shlomit Gilad
IPC分类号： C12N15/64 , C40B40/06 , C12N9/00 , C07H21/04
CPC分类号： C12N15/64 , C12N15/10 , C12N15/66
摘要： The present invention provides highly accurate methods for cloning nucleic acids in a desired, pre-defined orientation, and additionally provides for a library of cloned oriented nucleic acids. The ability to control the orientation of a nucleic acid being cloned is highly important in various molecular biology applications.
摘要翻译：本发明提供用于以期望的预定义取向克隆核酸的高度精确的方法，并另外提供克隆的定向核酸文库。控制被克隆的核酸取向的能力在各种分子生物学应用中是非常重要的。

5. 发明授权

US07524935B2 Sequences characteristic of hypoxia-regulated gene transcription 有权
标题翻译：缺氧调控基因转录特征的序列
公开(公告)号：US07524935B2
公开(公告)日：2009-04-28
申请号：US11440016
申请日：2006-05-25
申请人： Paz Einat , Rami Skaliter , Elena Feinstein , Aviv Regev , Hagar Kalinski
发明人： Paz Einat , Rami Skaliter , Elena Feinstein , Aviv Regev , Hagar Kalinski
IPC分类号： C07K16/00
CPC分类号： C12Q1/6883 , A61K38/00 , A61K48/00 , C07K14/47 , C07K14/4702 , C07K14/4747 , C12N9/0004 , C12Q2600/158 , C12Y114/11004
摘要： There are provided polynucleotides that are modulated by hypoxic conditions. The disclosure includes such genes and proteins as well as analogs, salts and functional derivatives of such proteins, and DNA encoding such analogs, and methods of use. Methods for treating the effects of stroke, hypoxia and/or ischemia by regulating such genes or proteins are also disclosed. The presence of hypoxia or a hypoxia-associated pathology may be diagnosed by screening for the presence of at least one polynucleotide having the nucleic acid sequence according to the present invention. Methods of regulating hypoxia associated pathologies are also provided.
摘要翻译：提供了由缺氧条件调节的多核苷酸。本公开包括此类基因和蛋白质以及此类蛋白质的类似物，盐和功能性衍生物，以及编码此类类似物的DNA及其使用方法。还公开了通过调节这些基因或蛋白质治疗中风，缺氧和/或缺血的作用的方法。可以通过筛选至少一种具有本发明的核酸序列的多核苷酸的存在来诊断缺氧或缺氧相关病理的存在。还提供了调节缺氧相关病变的方法。

6. 发明申请

US20090036662A1 Hypoxia-regulated genes 有权
标题翻译：低氧调控基因
公开(公告)号：US20090036662A1
公开(公告)日：2009-02-05
申请号：US12157821
申请日：2008-06-13
申请人： Paz Einat , Rami Skaliter , Sylvie Luria
发明人： Paz Einat , Rami Skaliter , Sylvie Luria
IPC分类号： C07H21/02
CPC分类号： C07K14/47 , A61K38/00 , A61K48/00 , C07K14/4702 , C07K14/4747
摘要： According to the present invention, purified, isolated and cloned nucleic acid polynucleotide encoding hypoxia-regulating genes and the proteins thereof and antibodies directed against the proteins which have sequences as set forth in SEQ ID No:1, SEQ ID No:2, SEQ ID No:3, SEQ ID No:4, SEQ ID No:5 and SEQ ID No:6 are provided. The present invention further provides transgenic animals and cell lines as well as knock-out organisms of these sequences. The present invention further provides methods of regulating angiogenesis or apoptosis or regulating response to hypoxic conditions in a patient in need of such treatment. The present invention also provides a method of diagnosing the presence of ischemia in a patient including the steps of analyzing a bodily fluid or tissue sample from the patient for the presence or gene product of at least one expressed gene (up-regulated) as set forth in the group comprising SEQ ID No:2; SEQ ID No:3; SEQ ID No:4; SEQ ID No:5; and SEQ ID No:6 and where ischemia is determined if the up-regulated gene or gene product is ascertained.
摘要翻译：根据本发明，编码缺氧调节基因及其蛋白质的纯化，分离和克隆的核酸多核苷酸和针对具有SEQ ID No：1，SEQ ID No：2，SEQ ID NO：2所示序列的蛋白质的抗体提供了No.3，SEQ ID No：4，SEQ ID No：5和SEQ ID No：6。本发明进一步提供这些序列的转基因动物和细胞系以及敲除生物体。本发明还提供了在需要这种治疗的患者中调节血管发生或凋亡或调节对缺氧条件的反应的方法。本发明还提供了一种诊断患者局部缺血的方法，包括以下步骤：从患者体内分析至少一种表达基因（上调）的存在或基因产物，如上所述在包含SEQ ID No：2的组中; SEQ ID No：3; SEQ ID No：4; SEQ ID No：5; 和SEQ ID No：6，如果确定上调基因或基因产物，则确定缺血。

7. 发明授权

US06555667B1 Hypoxia-regulated genes 有权
标题翻译：低氧调控基因
公开(公告)号：US06555667B1
公开(公告)日：2003-04-29
申请号：US09604728
申请日：2000-06-28
申请人： Paz Einat , Rami Skaliter
发明人： Paz Einat , Rami Skaliter
IPC分类号： C07H2104
CPC分类号： C07K14/4747 , A61K38/00 , A61K48/00 , C07K14/47 , C07K14/4702
摘要： Neuroleukin (SEQ ID NO:8) has been discovered to be hypoxia regulated. Furthermore, it serves as an astrocyte motility factor. Astrocyte motility can be inhibited by a neuroleukin inhibitor.
摘要翻译：已经发现神经白细胞介素（SEQ ID NO：8）是缺氧调节的。此外，它作为星形胶质细胞运动因子。星形胶质细胞运动能被神经白细胞介素抑制剂抑制。

8. 发明申请

US20080300208A1 Isociatrate dehydrogenase and uses therof 审中-公开
标题翻译：联合脱氢酶并使用
公开(公告)号：US20080300208A1
公开(公告)日：2008-12-04
申请号：US12069188
申请日：2008-02-07
申请人： Paz Einat , Louis Deiss , Ruth Maya
发明人： Paz Einat , Louis Deiss , Ruth Maya
IPC分类号： A61K31/7088 , C07H21/04 , C12N15/85 , C12N5/08 , C12N5/06
CPC分类号： C07K16/40 , A61K2039/505
摘要： The present invention discloses uses for the IDH gene and/or polypeptide and/or modulators thereof in the diagnosis and treatment of apoptosis-related diseases.
摘要翻译：本发明公开了IDH基因和/或多肽和/或其调节剂在凋亡相关疾病的诊断和治疗中的用途。

9. 发明申请

US20070042418A1 Therapeutic modulation of the fas pathway 失效
标题翻译： fas途径的治疗调节
公开(公告)号：US20070042418A1
公开(公告)日：2007-02-22
申请号：US11586021
申请日：2006-10-24
申请人： Fruma Yehiely , Louis Deiss , Paz Einat
发明人： Fruma Yehiely , Louis Deiss , Paz Einat
IPC分类号： C12Q1/68
CPC分类号： A61K48/00
摘要： A method for the identification of genes that are essential for the maintenance of specific cell phenotypes is disclosed. The method includes the initial step of identifying a cell type with a phenotype of interest. Gene inactivation is performed on an aliquot of cells of the cell type of interest. Selection is then performed to an aliquot of the cell culture to which gene inactivation has been applied. Cells which continue to maintain the phenotype following gene inactivation have not had the gene of interest inactivated whereas cells in which genes necessary for maintaining the phenotype have been inactivated have been lost. Utilizing subtraction analysis between treated and untreated aliquots the gene in the cells which has been inactivated that affects the phenotype of interest is identified. Genes that are identified by the method are also disclosed as well as antibodies directed against the gene product of the identified genes. Further a customized kit for the practice of the gene identification method is also disclosed.
摘要翻译：公开了用于鉴定对维持特定细胞表型必不可少的基因的方法。该方法包括鉴定具有感兴趣的表型的细胞类型的初始步骤。对感兴趣的细胞类型的细胞的等分试样进行基因灭活。然后对已应用基因失活的细胞培养物的等分试样进行选择。在基因失活后继续保持表型的细胞没有使目的基因失活，而其中维持表型所必需的基因已被灭活的细胞已经丧失。鉴定了处理和未处理等分试样之间的减法分析，已经灭活的影响感兴趣的表型的细胞中的基因被鉴定出来。还公开了通过该方法鉴定的基因以及针对所鉴定的基因的基因产物的抗体。还公开了用于实施基因鉴定方法的定制试剂盒。

10. 发明授权

US07074895B2 Sequences characteristic of hypoxia-regulated gene transcription 有权
公开(公告)号：US07074895B2
公开(公告)日：2006-07-11
申请号：US09802472
申请日：2001-03-09
申请人： Paz Einat , Rami Skaliter , Elena Feinstein , Aviv Regev
发明人： Paz Einat , Rami Skaliter , Elena Feinstein , Aviv Regev
IPC分类号： C07K1/00 , C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6883 , A61K38/00 , A61K48/00 , C07K14/47 , C07K14/4702 , C07K14/4747 , C12N9/0004 , C12Q2600/158 , C12Y114/11004
摘要： There are provided polynucleotides which are modulated by hypoxic conditions. The disclosure includes such genes and proteins as well as analogs, salts and functional derivatives of such proteins, and DNA encoding such analogs, and methods of use. Methods for treating the effects of stroke, hypoxia and/or ischemia by regulating such genes or proteins are also disclosed. The presence of hypoxia or a hypoxia-associated pathology may be diagnosed by screening for the presence of at least one polynucleotide having the nucleic acid sequence according to the present invention. Methods of regulating hypoxia associated pathologies are also provided.

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