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1. 发明申请

WO2010111674A2 METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION 审中-公开
标题翻译：使用能量转移检测的单分子序列的方法和装置
公开(公告)号：WO2010111674A2
公开(公告)日：2010-09-30
申请号：PCT/US2010/028952
申请日：2010-03-26
申请人： LIFE TECHNOLOGIES CORPORATION , NIKIFOROV, Theo , BEECHEM, Joseph
发明人： NIKIFOROV, Theo , BEECHEM, Joseph
IPC分类号： C12Q1/68 , C07H21/00 , G01N33/52
CPC分类号： C12Q1/6869 , C07H19/20 , C12N9/1241 , C12N9/1252 , C12N9/96 , C12Q1/6818 , C12Y207/07 , C12Y207/07007 , G01N21/6428 , G01N33/582 , G01N2021/6432
摘要： Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.
摘要翻译：本文提供了用于核苷酸掺入反应的系统和方法。该系统包含具有改变的核苷酸掺入动力学并且与能量转移供体部分连接的聚合酶，以及与至少一个能量转移受体部分连接的核苷酸分子。当核苷酸结合和/或核苷酸掺入期间聚合酶和核苷酸彼此接近时，供体和受体部分进行能量转移。当供体和受体部分进行能量转移时，它们产生可与核苷酸结合或掺入相关联的能量转移信号。检测所产生的信号的时间序列或信号的变化可用于确定掺入的核苷酸的顺序，因此可用于推导靶分子的序列。

2. 发明申请

WO2012109315A1 LINKING METHODS, COMPOSITIONS, SYSTEMS, KITS AND APPARATUSES 审中-公开
标题翻译：链接方法，组合，系统，工具和设备
公开(公告)号：WO2012109315A1
公开(公告)日：2012-08-16
申请号：PCT/US2012/024266
申请日：2012-02-08
申请人： LIFE TECHNOLOGIES CORPORATION , DAVIDSON, John , NIKIFOROV, Theo , LUO, Guobin
发明人： DAVIDSON, John , NIKIFOROV, Theo , LUO, Guobin
IPC分类号： G01N33/535 , G01N33/543 , G01N33/58
CPC分类号： C12N11/10 , C12N9/1241 , C12Q1/68 , G01N2333/9126
摘要： In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.
摘要翻译：在一些实施方案中，本公开一般涉及方法以及相关组合物，系统，试剂盒和装置，其包括使用系链将蛋白质与靶化合物和/或感兴趣的位置连接。例如，系链可用于将蛋白质连接到目标化合物，例如将酶连接至底物。类似地，系链可用于将蛋白质链接在表面上的期望位置处或附近。在一组实施方案中，系链包括多核苷酸，并且目标化合物或表面上的位置包括能够与系链杂交的另一多核苷酸。在这样的实施方案中，系链可用于使用核酸杂交将蛋白质与靶化合物或位置连接。

3. 发明申请

WO2015048763A1 POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开
标题翻译：聚合物组合物，制备方法和使用方法
公开(公告)号：WO2015048763A1
公开(公告)日：2015-04-02
申请号：PCT/US2014/058361
申请日：2014-09-30
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： VANDER HORN, Peter , MAZUR, Daniel , NIKIFOROV, Theo , LANDES, Mindy , TOZER, Eileen
IPC分类号： C12Q1/68 , C12N9/12
CPC分类号： C12N9/1252 , C12Q1/686 , C12Q1/6869
摘要： The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.
摘要翻译：本公开提供了可用于核酸聚合的组合物，方法，试剂盒，系统和装置。特别地，提供了允许核酸扩增的修饰的聚合酶及其生物活性片段。在一些方面，本公开提供与参考聚合酶相比具有较低系统误差的修饰聚合酶。一方面，本公开涉及可用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式遗传分析的修饰聚合酶。在一些方面，本公开涉及可用于产生核酸文库或核酸模板的修饰聚合酶。在一些方面，本公开涉及可以跨越聚合酶类或家族转移的同源氨基酸突变的鉴定，以提供具有改变的性质的新型聚合酶。

4. 发明申请

WO2013019361A1 SEQUENCING METHODS 审中-公开
标题翻译：序列方法
公开(公告)号：WO2013019361A1
公开(公告)日：2013-02-07
申请号：PCT/US2012/045783
申请日：2012-07-06
申请人： LIFE TECHNOLOGIES CORPORATION , MYERS, Jason , CHEN, Zhoutao , DRESSMAN, Devin , NIKIFOROV, Theo
发明人： MYERS, Jason , CHEN, Zhoutao , DRESSMAN, Devin , NIKIFOROV, Theo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q1/6806 , C12Q1/6869 , C12Q2521/307 , C12Q2521/325 , C12Q2525/125 , C12Q2535/119 , C12Q2565/518
摘要： Provided herein are compositions, systems, methods and kits useful for obtaining sequence information from a nucleic acid molecule. In some embodiments, the compositions, systems, methods and kits are useful for sequencing of natural or modified nucleic acids. In some embodiments, the compositions, systems, methods and kits relate to bi-directional sequencing of nucleic acids. In some embodiments, the compositions, systems, methods and kits relate to sequencing of nucleic acids linked to a solid support. In some embodiments, the methods useful for obtaining sequence information from a nucleic acid molecule include label-free or ion-based sequencing methods.
摘要翻译：本文提供了可用于从核酸分子获得序列信息的组合物，系统，方法和试剂盒。在一些实施方案中，组合物，系统，方法和试剂盒可用于测序天然或修饰的核酸。在一些实施方案中，组合物，系统，方法和试剂盒涉及核酸的双向测序。在一些实施方案中，组合物，系统，方法和试剂盒涉及与固体支持物连接的核酸的测序。在一些实施方案中，可用于从核酸分子获得序列信息的方法包括无标记或基于离子的测序方法。

5. 发明申请

WO2017214561A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：核酸扩增的方法和组合物
公开(公告)号：WO2017214561A1
公开(公告)日：2017-12-14
申请号：PCT/US2017/036842
申请日：2017-06-09
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： YU, Hua , NIKIFOROV, Theo , ROSENBAUM, Abraham
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68
摘要： In some embodiments, the disclosure relates generally to methods, as well as related compositions and kits for recombinase-mediated nucleic acid amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using at least one blocked primer that contains a 5' domain, at least one nucleotide that is cleavable by an RNase H enzyme, a 3' domain, wherein the primer is not extendable by a polymerase, and wherein the 3' domain has a length of 7-100 nucleotides, for example 10-30 nucleotides. These methods and the use of a blocked primer reduce or eliminate non-specific amplification products, such as primer dimers, which are generated in RPA reactions.
摘要翻译：在一些实施方案中，本公开内容一般涉及使用核酸模板的核酸模板的用于重组酶介导的核酸扩增例如重组酶 - 聚合酶扩增（RPA）的方法以及相关组合物和试剂盒至少一种含有5'结构域的封闭引物，至少一个可被RNase H酶切割的核苷酸，3'结构域，其中引物不能被聚合酶延伸，并且其中3'结构域的长度为 7-100个核苷酸，例如10-30个核苷酸。这些方法和封闭引物的使用减少或消除在RPA反应中产生的非特异性扩增产物，例如引物二聚体。

6. 发明申请

WO2013023176A2 POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-公开
标题翻译：聚合酶组合物，其制备和使用方法
公开(公告)号：WO2013023176A2
公开(公告)日：2013-02-14
申请号：PCT/US2012/050423
申请日：2012-08-10
申请人： LIFE TECHNOLOGIES CORPORATION , VANDER HORN, Peter , NIKIFOROV, Theo , LUO, Guobin , LANDES, Mindy , MAZUR, Daniel , TOZER, Eileen , LINCECUM, Tommie
发明人： VANDER HORN, Peter , NIKIFOROV, Theo , LUO, Guobin , LANDES, Mindy , MAZUR, Daniel , TOZER, Eileen , LINCECUM, Tommie
IPC分类号： C12Q1/68
CPC分类号： C12N9/1252 , C12Q1/6846 , C12Q1/686 , C12Y207/07007
摘要： The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.
摘要翻译：本公开提供了用于核酸聚合的组合物，方法，试剂盒，系统和装置。具体而言，提供了允许核酸扩增的修饰聚合酶及其生物活性片段。在一个方面，本公开涉及用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式的遗传分析的修饰的聚合酶。在一些方面，本公开涉及用于产生供各种下游过程使用的核酸文库或核酸模板的修饰聚合酶。在一些方面，本公开涉及鉴定可以跨聚合酶的类或家族转移以提供具有改变的催化性质的新聚合酶的同源氨基酸突变。在一些方面，本公开内容提供了与参考聚合酶相比具有增强的催化性质的修饰聚合酶。

7. 发明申请

WO2016077324A1 THIOLATED NUCLEOTIDE ANALOGUES FOR NUCLEIC ACID SYNTHESIS 审中-公开
标题翻译：用于核酸合成的硫酸核苷酸类似物
公开(公告)号：WO2016077324A1
公开(公告)日：2016-05-19
申请号：PCT/US2015/059921
申请日：2015-11-10
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： MENCHEN, Steven , NIKIFOROV, Theo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , G01N27/4145 , C12Q2525/125 , C12Q2535/113 , C12Q2565/301 , C12Q2565/607
摘要： The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.
摘要翻译：本公开提供用于扩展核酸的系统，组合物，方法，试剂，试剂盒和产品，其包括使用聚合酶和至少一个核苷酸在核酸的末端掺入核苷酸残基，其中所述至少一个核苷酸包括硫代磷酸酯部分，并且其中所述至少一个核苷酸耐磷酸酶水解。在一些实施方案中，核苷酸掺入可以在磷酸酶的存在下进行。在一些实施方案中，核苷酸掺入可以在配置成结合正磷酸盐部分的至少一个螯合部分的存在下进行。

8. 发明公开

EP4219739A3 POLYMERASE COMPOSITIONS, METHODS OF MAKING AND USING SAME 审中-实审
公开(公告)号：EP4219739A3
公开(公告)日：2023-08-23
申请号：EP22208638.1
申请日：2014-09-30
申请人： Life Technologies Corporation
发明人： VANDER HORN, Peter , MAZUR, Daniel , NIKIFOROV, Theo , LANDES, Mindy , TOZER, Eileen
IPC分类号： C12Q1/68 , C12N9/12
摘要： The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. In some aspects, the disclosure provides modified polymerases having lower systematic error as compared to a reference polymerase. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered properties.

9. 发明公开

EP3293274A3 POLYMERASE COMPOSITIONS 有权
标题翻译：聚合酶组合物
公开(公告)号：EP3293274A3
公开(公告)日：2018-03-28
申请号：EP17198055.0
申请日：2012-08-10
申请人： Life Technologies Corporation
发明人： VANDER HORN, Peter , NIKIFOROV, Theo , LUO, Guobin , LANDES, Mindy , MAZUR, Daniel , TOZER, Eileen , LINCECUM, Tommie
IPC分类号： C12Q1/68 , C12N9/12
摘要： The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.
摘要翻译：本公开提供可用于核酸聚合的组合物，方法，试剂盒，系统和装置。具体而言，提供了允许核酸扩增的修饰聚合酶及其生物活性片段。在一个方面，本公开涉及用于核酸测序，基因分型，拷贝数变异分析，配对末端测序和其他形式的遗传分析的修饰的聚合酶。在一些方面，本公开涉及用于产生供各种下游过程使用的核酸文库或核酸模板的修饰聚合酶。在一些方面，本公开涉及鉴定可以跨聚合酶的类或家族转移以提供具有改变的催化性质的新聚合酶的同源氨基酸突变。在一些方面，本公开提供了与参考聚合酶相比具有增强的催化性质的修饰的聚合酶。

10. 发明公开

EP3293274A2 POLYMERASE COMPOSITIONS 有权
公开(公告)号：EP3293274A2
公开(公告)日：2018-03-14
申请号：EP17198055.0
申请日：2012-08-10
申请人： Life Technologies Corporation
发明人： VANDER HORN, Peter , NIKIFOROV, Theo , LUO, Guobin , LANDES, Mindy , MAZUR, Daniel , TOZER, Eileen , LINCECUM, Tommie
IPC分类号： C12Q1/68 , C12N9/12
摘要： The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. In one aspect, the disclosure relates to modified polymerases useful for nucleic acid sequencing, genotyping, copy number variation analysis, paired-end sequencing and other forms of genetic analysis. In some aspects, the disclosure relates to modified polymerases useful for the generation of nucleic acid libraries or nucleic acid templates for use in various downstream processes. In some aspects, the disclosure relates to the identification of homologous amino acid mutations that can be transferred across classes or families of polymerases to provide novel polymerases with altered catalytic properties. In some aspects, the disclosure provides modified polymerases having enhanced catalytic properties as compared to a reference polymerase.

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