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1. 发明申请

WO2020037284A1 SYSTEM AND METHOD FOR PREPARING A SEQUENCING DEVICE 审中-公开
公开(公告)号：WO2020037284A1
公开(公告)日：2020-02-20
申请号：PCT/US2019/046941
申请日：2019-08-16
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： YU, Hua , ZHENG, Rui , YANG, Xiaoling , WONG, Chiu Tai Andrew , GRAY, Jeremy
IPC分类号： C12Q1/6806 , C12Q1/6874 , B01L3/00 , C12Q1/6844 , C40B50/14 , G01N35/00
摘要： The disclosure generally relates to systems, methods, and apparatuses for magnetic bead loading. An example embodiment of the disclosure relates to mixing magnetic beads with sequencing beads to form a solution. The solution containing both beads is injected onto a microchip having a plurality of microwells. The magnetic beads may have larger diameter than the microwell while the sequencing beads may have a smaller diameter, allowing them to enter and reside in the microwell. One or more magnets positioned under the microchip move back and forth across the microchip surface. The magnetic beads form a line and follow the movement of the magnets. During rounds of sweeping, the sequencing beads load into the respective wells. The magnets may be disengaged and the magnetic beads may be washed away after the sequencing beads are loaded.

2. 发明申请

WO2019094524A1 METHODS AND COMPOSITIONS FOR MANIPULATING NUCLEIC ACIDS 审中-公开
公开(公告)号：WO2019094524A1
公开(公告)日：2019-05-16
申请号：PCT/US2018/059710
申请日：2018-11-07
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： ROSENBAUM, Abraham , SEEGER, Collyn , GRAY, Jeremy , YU, Hua
IPC分类号： C12Q1/6806 , C12Q1/6874
CPC分类号： C12Q1/686 , C12Q1/6806 , C12Q1/6834 , C12Q1/6853 , C12Q1/6874 , C12Q1/6876 , C12Q2600/16 , C12Q2525/197 , C12Q2535/122 , C12Q2563/143 , C12Q2563/149 , C12Q2565/519
摘要： The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

3. 发明申请

WO2017214561A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：核酸扩增的方法和组合物
公开(公告)号：WO2017214561A1
公开(公告)日：2017-12-14
申请号：PCT/US2017/036842
申请日：2017-06-09
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： YU, Hua , NIKIFOROV, Theo , ROSENBAUM, Abraham
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68
摘要： In some embodiments, the disclosure relates generally to methods, as well as related compositions and kits for recombinase-mediated nucleic acid amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using at least one blocked primer that contains a 5' domain, at least one nucleotide that is cleavable by an RNase H enzyme, a 3' domain, wherein the primer is not extendable by a polymerase, and wherein the 3' domain has a length of 7-100 nucleotides, for example 10-30 nucleotides. These methods and the use of a blocked primer reduce or eliminate non-specific amplification products, such as primer dimers, which are generated in RPA reactions.
摘要翻译：在一些实施方案中，本公开内容一般涉及使用核酸模板的核酸模板的用于重组酶介导的核酸扩增例如重组酶 - 聚合酶扩增（RPA）的方法以及相关组合物和试剂盒至少一种含有5'结构域的封闭引物，至少一个可被RNase H酶切割的核苷酸，3'结构域，其中引物不能被聚合酶延伸，并且其中3'结构域的长度为 7-100个核苷酸，例如10-30个核苷酸。这些方法和封闭引物的使用减少或消除在RPA反应中产生的非特异性扩增产物，例如引物二聚体。

4. 发明公开

EP3469078A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 有权
公开(公告)号：EP3469078A1
公开(公告)日：2019-04-17
申请号：EP17811116.7
申请日：2017-06-09
申请人： Life Technologies Corporation
发明人： YU, Hua , NIKIFOROV, Theo , ROSENBAUM, Abraham
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68

5. 发明公开

EP3707274A1 METHODS AND COMPOSITIONS FOR MANIPULATING NUCLEIC ACIDS 有权
公开(公告)号：EP3707274A1
公开(公告)日：2020-09-16
申请号：EP18808182.2
申请日：2018-11-07
申请人： Life Technologies Corporation
发明人： ROSENBAUM, Abraham , SEEGER, Collyn , GRAY, Jeremy , YU, Hua
IPC分类号： C12Q1/6806 , C12Q1/6874

6. 发明公开

EP3963103A1 METHODS AND COMPOSITIONS FOR MANIPULATING NUCLEIC ACIDS 有权
公开(公告)号：EP3963103A1
公开(公告)日：2022-03-09
申请号：EP20729277.2
申请日：2020-04-30
申请人： Life Technologies Corporation
发明人： ROSENBAUM, Abraham , SEEGER, Collyn , GRAY, Jeremy , YU, Hua
IPC分类号： C12Q1/6853

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