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1. 发明申请

WO2013119936A2 HYDROPHILIC POLYMERIC PARTICLES AND METHODS FOR MAKING SAME 审中-公开
标题翻译：水解聚合物颗粒及其制备方法
公开(公告)号：WO2013119936A2
公开(公告)日：2013-08-15
申请号：PCT/US2013/025328
申请日：2013-02-08
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： FONNUM, Geir , MODAHL, Grete , KJUS, Nini , MOLTEBERG, Astrid Evenroed , TRAN, Diem , AASERUD, Jo , GOKMEN, M. Talha , MENCHEN, Steven , FULLER, Carl , ANDRUZZI, Luisa , HINZ, Wolfgang , GREMYACHINSKIY, Dmitriy , LIGHT, David , MASTROIANNI, Alexander , ROSENBLUM, Barnett , KHAN, Shaheer , STOLARCZYK, Craig , SOKOLSKY, Tanya
IPC分类号： C08F2/16 , C08F230/08
CPC分类号： C08F130/08 , C07F7/1852 , C07F7/188 , C08F2/20 , C08F2/26 , C08F6/24 , C08F8/12 , C08F8/32 , C08F8/34 , C08F220/58 , C08F222/385 , C08F230/08 , C08F2800/20 , C08J9/20 , C08J2343/04 , C12P19/34 , C12Q1/686 , C12Q1/6874 , G01N27/4145 , Y10T428/2982 , C08F257/02 , C08F2222/1013 , C08F212/36 , C08F220/60 , C12Q2563/149 , C12Q2563/159 , C12Q2565/543
摘要： A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.
摘要翻译：形成颗粒的方法包括在水性悬浮液中的分散相中聚合具有疏水保护基团的亲水性单体的多个聚合单元，从而形成包含多个疏水保护基团的聚合物颗粒。该方法还包括将聚合物颗粒转化为亲水性颗粒。

2. 发明申请

WO2016153999A1 MODIFIED NUCLEOTIDES AND USES THEREOF 审中-公开
标题翻译：改良的核苷酸及其用途
公开(公告)号：WO2016153999A1
公开(公告)日：2016-09-29
申请号：PCT/US2016/023139
申请日：2016-03-18
申请人： LIFE TECHNOLOGIES CORPORATION , LIFE TECHNOLOGIES GMBH
发明人： HINZ, Wolfgang , MENCHEN, Steven , GRAHAM, Ronald , VANDER HORN, Peter , HUBBELL, Earl , WOEHLER, Christian
IPC分类号： C12Q1/68 , G01N27/414
CPC分类号： C12Q1/6869 , G01N27/4145 , C12Q2525/117 , C12Q2565/30
摘要： In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. Optionally, the array contains a plurality of reaction sites having about 1-100 million, or about 100-250 million, or about 200-500 million, or about 500-900 million, or more reaction sites. Optionally, each reaction site is in contact with, operatively coupled, or capacitively coupled to one or more sensors that are ion-sensitive FETs (isFETs) or chemically-sensitive FETs (chemFETs) sensors. Optionally, the reaction sites are in fluid communication with each other.
摘要翻译：在一些实施方案中，本公开一般涉及涉及在模板依赖性中使用经修饰的核苷酸（包括终止子核苷酸和/或标记的核苷酸）的核酸分析的方法以及相关的系统，组合物，试剂盒和装置核苷酸掺入反应。在一些实施方案中，核酸分析可以在单个反应位点或反应位点阵列中的多个反应位点进行。任选地，阵列包含多个具有约1-100百万，约100-250百万，或约200-500百万，或约500-900万个或更多个反应位点的反应位点。任选地，每个反应位点与作为离子敏感FET（isFET）或化学敏感FET（chemFET）传感器的一个或多个传感器接触，可操作地耦合或电容耦合。任选地，反应位点彼此流体连通。

3. 发明申请

WO2022020731A2 COMPOSITIONS, SYSTEMS AND METHODS FOR BIOLOGICAL ANALYSIS USING DYES 审中-公开
公开(公告)号：WO2022020731A2
公开(公告)日：2022-01-27
申请号：PCT/US2021/043000
申请日：2021-07-23
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： BENSON, Scott , MULLAH, Khairuzzaman Bashar , CHANG, Chu-An , MENCHEN, Steven , FREUDENTHAL, Jacob , LEE, Linda
IPC分类号： G01N21/64 , C12Q1/68 , C12Q1/686 , C12Q1/6851 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6441 , G01N21/6428
摘要： A system (1000) comprising first and second excitation sources (101a, 101b) with respective excitation wavelengths for exciting first second and third dyes in a sample (110) and further comprising a detector (115), first and second emission spectral elements (121a, 121b) for transmitting respective first and second emission wavelengths as well as a processor (130) for automatically operating the elements of the system. The first dye comprises a first absorption spectrum comprising a first maximum absorption wavelength and the second dye comprises a second absorption spectrum comprising a second maximum absorption wavelength that is equal to or substantially equal to the first maximum absorption wavelength. The second dye comprises a second emission spectrum comprising a second maximum emission wavelength and the third dye comprises a third emission spectrum comprising a third maximum emission wavelength that is equal to or substantially equal to the second maximum emission wavelength.

4. 发明申请

WO2017196676A1 METAL CHELATION POST INCORPORATION DETECTION METHODS 审中-公开
标题翻译：金属螯合后检测方法
公开(公告)号：WO2017196676A1
公开(公告)日：2017-11-16
申请号：PCT/US2017/031403
申请日：2017-05-05
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： WONG, Chiu Tai Andrew , YANG, Xiaoling , SEEGER, Collyn , REARICK, Todd , MENCHEN, Steven , ROZHKOV, Roman , ROSENBLUM, Barnett , GRAHAM, Ronald
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2563/137 , C12Q2563/149 , C12Q2565/607
摘要： The present disclosure provides methods relating to detecting, identifying and measuring nucleic acids that includes binding metal ions to nucleic acid polymer backbones having negative charges formed as a result of nucleotide incorporation, and detecting or measuring hydrogen ions that are generated as a result of forming metal chelate complexes by addition of chelating agents.
摘要翻译：本公开提供了涉及检测，鉴定和测量核酸的方法，所述方法包括将金属离子结合至具有由于核苷酸掺入而形成负电荷的核酸聚合物骨架，并且检测或测量氢离子是由于通过添加螯合剂形成金属螯合物而产生的。

5. 发明申请

WO2013119956A1 CONJUGATED POLYMERIC PARTICLE AND METHOD OF MAKING SAME 审中-公开
标题翻译：聚合聚合物颗粒及其制备方法
公开(公告)号：WO2013119956A1
公开(公告)日：2013-08-15
申请号：PCT/US2013/025352
申请日：2013-02-08
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： MENCHEN, Steven , BLANCHARD, Alan , ANDRUZZI, Luisa , KHAN, Shaheer , GREMYACHINSKIY, Dmitriy , LUI, Alfred , STOLARCZYK, Craig , SOKOLSKY, Tanya
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C08F8/40 , C12Q1/6834 , C12Q2523/101 , C12Q2563/149 , C12Q2565/119
摘要： A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a non¬ aqueous solvent, and coupling the biomolecule complex to a sub¬ strate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immo¬ bilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.
摘要翻译：缀合底物的方法包括将与生物分子相关的抗衡离子与亲脂性反离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在存在下将生物分子复合物偶联到亚基的非水溶剂。底物或固体支持物是聚合物颗粒，待沉淀在颗粒上的生物分子是寡核苷酸，亲脂性抗衡离子是四丁基铵离子，非水溶剂是N-甲基吡咯烷酮（NMP）。寡核苷酸包被的颗粒用于核酸分离或核酸测序反应。

6. 发明申请

WO2022020723A1 ENERGY TRANSFER DYE CONJUGATES FOR USE IN BIOLOGICAL ASSAYS 审中-公开
公开(公告)号：WO2022020723A1
公开(公告)日：2022-01-27
申请号：PCT/US2021/042982
申请日：2021-07-23
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： BENSON, Scott , MULLAH, Khairuzzaman , CHANG, Chu-An , MENCHEN, Steven , LEE, Linda
IPC分类号： C07H21/00 , C12Q1/6818
摘要： Energy transfer dye pairs including a donor dye covalently attached to an acceptor dye through a linker, uses of the energy transfer dye pairs, for example, in conjugates of an energy transfer dye pair covalently attached to a quencher and an analyte (e.g., an oligonucleotide), for biological applications including, for example, amplification assays such as quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR).

7. 发明申请

WO2018237024A1 MESYLATE BASED MASTER MIX 审中-公开
公开(公告)号：WO2018237024A1
公开(公告)日：2018-12-27
申请号：PCT/US2018/038531
申请日：2018-06-20
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： ZHONG, Chang , MULERO, Julio , MENCHEN, Steven
IPC分类号： C12Q1/686 , C12Q1/6848
摘要： Disclosed herein are mesylate containing compositions, methods of use of such compositions and kits of components encompassing such compositions for improving the amplification of nucleic acids, especially in the presence of enzymatic inhibitors. These find particular use in forensic and environmental analyses. The instant disclosure provides new compositions, methods and kits of components for overcoming PCR inhibition. In some embodiments disclosed herein is a PCR master mix, the PCR master mix encompassing a mesylate.

8. 发明申请

WO2016077324A1 THIOLATED NUCLEOTIDE ANALOGUES FOR NUCLEIC ACID SYNTHESIS 审中-公开
标题翻译：用于核酸合成的硫酸核苷酸类似物
公开(公告)号：WO2016077324A1
公开(公告)日：2016-05-19
申请号：PCT/US2015/059921
申请日：2015-11-10
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： MENCHEN, Steven , NIKIFOROV, Theo
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , G01N27/4145 , C12Q2525/125 , C12Q2535/113 , C12Q2565/301 , C12Q2565/607
摘要： The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety.
摘要翻译：本公开提供用于扩展核酸的系统，组合物，方法，试剂，试剂盒和产品，其包括使用聚合酶和至少一个核苷酸在核酸的末端掺入核苷酸残基，其中所述至少一个核苷酸包括硫代磷酸酯部分，并且其中所述至少一个核苷酸耐磷酸酶水解。在一些实施方案中，核苷酸掺入可以在磷酸酶的存在下进行。在一些实施方案中，核苷酸掺入可以在配置成结合正磷酸盐部分的至少一个螯合部分的存在下进行。

9. 发明申请

WO2013119894A1 HYDROPHOBIC DIACRYLAMIDE COMPOUND 审中-公开
标题翻译：疏水性二丙烯酰胺化合物
公开(公告)号：WO2013119894A1
公开(公告)日：2013-08-15
申请号：PCT/US2013/025259
申请日：2013-02-08
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： KJUS, Nini , FONNUM, Geir , BRANCHAUD, Bruce , YING, Lai-Qiang , MENCHEN, Steven , GREMYACHINSKIY, Dmitriy , KANG, Hee Chol
IPC分类号： C07F7/18 , C08F222/38
CPC分类号： C08F230/08 , C07F7/10 , C07F7/1804 , C07F7/1852 , C08F220/58 , C08F222/385 , C08F236/20 , C08K3/20
摘要： A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.
摘要翻译：描述了甲硅烷基保护的二丙烯酰胺化合物。形成这种化合物的方法包括在第一反应条件下将甲硅烷基化试剂与羟基化二胺化合物混合以在第一溶液中形成产物，将产物与第一溶液分离，并在第二反应条件下将产物与丙烯酰氯混合形成甲硅烷基保护的二丙烯酰胺化合物的第二溶液。

10. 发明申请

WO2012142132A1 POLYMER PARTICLES AND METHODS OF MAKING AND USING SAME 审中-公开
标题翻译：聚合物颗粒及其制备方法和使用方法
公开(公告)号：WO2012142132A1
公开(公告)日：2012-10-18
申请号：PCT/US2012/033084
申请日：2012-04-11
申请人： LIFE TECHNOLOGIES CORPORATION , MENCHEN, Steven , MASTROIANNI, Alexander
发明人： MENCHEN, Steven , MASTROIANNI, Alexander
IPC分类号： B01J13/00 , C08J3/00
CPC分类号： C12N15/1093 , C08F2/22 , C08F220/56 , C08L33/26 , Y10T428/2982
摘要： A method of making polymer particles includes making an aqueous gel reaction mixture; forming an emulsion comprising dispersed aqueous phase micelles of gel reaction mixture in a continuous phase at a temperature less than about 10°C; and performing a polymerization reaction in the micelles. Further, the emulsion comprises at least one polymerization initiator in the micelles of gel reaction mixture. The gel reaction mixture can be maintained at a temperature less than about 10°C when it comprises the polymerization initiator.
摘要翻译：制备聚合物颗粒的方法包括制备水凝胶反应混合物; 在小于约10℃的温度下，在连续相中形成包含分散的凝胶反应混合物的水相胶束的乳液; 并在胶束中进行聚合反应。此外，乳液在凝胶反应混合物的胶束中包含至少一种聚合引发剂。当凝胶反应混合物包含聚合引发剂时，可将其保持在低于约10℃的温度。

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