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1. 发明授权

US07947876B2 Plant and plant storage organ having GLP-1 derivative accumulated therein and method of producing the same 失效
标题翻译：蓄积有GLP-1衍生物的植物贮藏器具及其制造方法
公开(公告)号：US07947876B2
公开(公告)日：2011-05-24
申请号：US11662650
申请日：2004-09-14
申请人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Humio Takaiwa , Hiroshi Yasuda , Takahito Jomori , Yuji Hayashi , Akira Tashita
发明人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Humio Takaiwa , Hiroshi Yasuda , Takahito Jomori , Yuji Hayashi , Akira Tashita
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： A61K38/26 , A23L33/105 , C12N15/8257
摘要： The present invention is regarding plants and plant storage organs thereof in which GLP-1 derivatives are accumulated, and methods of producing them. The transgenic plants and plant storage organs thereof accumulate tandem repeated GLP-1 derivatives cleavable with intestinal digestive enzyme to monomeric molecules and are produced by methods comprising: integrating into vectors linked DNAs which comprise tandem repeated DNAs encoding the GLP-1 derivative with trypsin resistance in which the amino acid in the 26th position is Gln, the amino acid in the 34th position is Asn or Asp, and C-terminal consists of Arg or Lys to produce monomeric molecules; introducing the vectors into plant cells; and redifferentiating the obtained transformants. The edible transgenic plants and plant storage organs are useful for treating diabetes and can be ingested by diabetic patients.
摘要翻译：本发明涉及其中积累GLP-1衍生物的植物和植物贮藏器官及其生产方法。其转基因植物和植物储存器官将可与肠消化酶切割的串联重复的GLP-1衍生物积聚到单体分子中，并通过以下方法产生：包括：将包含编码GLP-1衍生物的串联重复DNA与胰蛋白酶抗性的连锁DNA连接其中第26位的氨基酸是Gln，第34位的氨基酸是Asn或Asp，C末端由Arg或Lys组成以产生单体分子; 将载体导入植物细胞; 并重新分化获得的转化体。可食用的转基因植物和植物贮藏器官可用于治疗糖尿病并可被糖尿病患者摄入。

2. 发明申请

US20070033676A1 Process for producing plant storage organ with high production of recombinant protein and novel recombinant protein 失效
标题翻译：生产重组蛋白和新型重组蛋白的植物贮藏器具生产工艺
公开(公告)号：US20070033676A1
公开(公告)日：2007-02-08
申请号：US10550624
申请日：2004-03-26
申请人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Humio Takaiwa , Takahito Jomori , Yuji Hayashi , Akira Tashita , Yukari Kobara
发明人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Humio Takaiwa , Takahito Jomori , Yuji Hayashi , Akira Tashita , Yukari Kobara
IPC分类号： A01H5/00 , C12N15/82 , A01H1/00 , C07K14/605
CPC分类号： C12N15/8257 , C07K14/605 , C12N15/8216
摘要： The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the cytokinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.
摘要翻译：本发明提供了在植物贮藏器官和GLP-1衍生物中高度产生重组蛋白的方法。通过使用包含重组蛋白基因，细胞分裂素相关基因，耐药性基因和可移除DNA元件的载体转化获得重组蛋白质高度产生的植物贮藏器官，其中细胞分裂素相关基因和耐药基因存在于与DNA元件一起表现的位置，而在植物储存器官中表达的重组蛋白存在于与DNA不一致的位置元件。通过使用该方法制备GLP-1，进一步提供了稳定的酶促消化的衍生物。

3. 发明申请

US20080292732A1 Plant and Plant Storage Organ Having Glp-1 Derivative Accumulated Therein and Method of Producing the Same 失效
标题翻译：含有Glp-1衍生物的植物和植物储存器官及其生产方法
公开(公告)号：US20080292732A1
公开(公告)日：2008-11-27
申请号：US11662650
申请日：2004-09-14
申请人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa , Hiroshi Yasuda , Takahito Jomori , Yuji Hayashi , Akira Tashita
发明人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa , Hiroshi Yasuda , Takahito Jomori , Yuji Hayashi , Akira Tashita
IPC分类号： A61K36/00 , C12N15/82 , A01H5/00 , A61P31/10
CPC分类号： A61K38/26 , A23L33/105 , C12N15/8257
摘要： The present invention is to provide a plant and a plant storage organ thereof in which GLP-1 derivatives are accumulated, and a method of producing them in order to develop a method for ingesting orally GLP-1 derivatives at a low cost and to make use of it in diabetic treatment. A transgenic plant or a plant storage organ thereof in which GLP-1 derivatives are accumulated cleavable with a digestive enzyme is produced by a method comprising: integrating into a vector a linked GLP-1s-DNA which comprises tandem repeated “n” DNAs (“n” being an integer of 3 or more) encoding a GLP-1 derivative consisting of GLP-1 (7-36) or of an amino acid sequence of GLP-1 (7-36) in which one or a few amino acids are deleted, substituted and/or added, and C-terminal consists of Arg or Lys, and having GLP-1 activity; introducing the vector into a plant cell; and redifferentiating the obtained transformant. The transgenic plant and the plant storage organ are useful as a pharmaceutical composition or a food or drink for treating or preventing diabetes.
摘要翻译：本发明提供一种其中积聚有GLP-1衍生物的植物和植物贮藏器官及其生产方法，以便开发低成本摄取口服GLP-1衍生物的方法并使用的糖尿病治疗。 GLP-1衍生物可以用消化酶积聚可切割的转基因植物或植物贮藏器官是通过以下方法产生的：将连接的GLP-1s-DNA整合到载体中，所述连接的GLP-1s-DNA包含串联重复的“n”DNA（“ 编号由GLP-1（7-36）或GLP-1（7-36）的氨基酸序列组成的GLP-1衍生物的n“为3以上的整数，其中一个或几个氨基酸为缺失，取代和/或添加，C-末端由Arg或Lys组成，具有GLP-1活性; 将载体导入植物细胞; 并对所得转化体进行重新分化。转基因植物和植物贮藏器官可用作药物组合物或用于治疗或预防糖尿病的食物或饮料。

4. 发明授权

US07847063B2 GLP-1 derivative 失效
标题翻译： GLP-1衍生物
公开(公告)号：US07847063B2
公开(公告)日：2010-12-07
申请号：US10550624
申请日：2004-03-26
申请人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa , Takahito Jomori , Yuji Hayashi , Akira Tashita , Yukari Kobara
发明人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa , Takahito Jomori , Yuji Hayashi , Akira Tashita , Yukari Kobara
IPC分类号： A61K38/00 , C07K14/00
CPC分类号： C12N15/8257 , C07K14/605 , C12N15/8216
摘要： The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the cytokinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.
摘要翻译：本发明提供了在植物贮藏器官和GLP-1衍生物中高度产生重组蛋白的方法。通过使用包含重组蛋白基因，细胞分裂素相关基因，耐药性基因和可移除DNA元件的载体转化获得重组蛋白质高度产生的植物贮藏器官，其中细胞分裂素相关基因和耐药基因存在于可以与DNA元件一起表现的位置，而在植物储存器官中表达的重组蛋白存在于与DNA不一致的位置元件。通过使用该方法制备GLP-1，进一步提供了稳定的酶促消化的衍生物。

5. 发明申请

US20070192906A1 Rice plant having vaccine gene transferred thereinto 审中-公开
标题翻译：具有转移疫苗基因的水稻植物
公开(公告)号：US20070192906A1
公开(公告)日：2007-08-16
申请号：US11547910
申请日：2005-04-08
申请人： Yoshikazu Yuki , Hiroshi Kiyono , Takachika Hiroi , Tomonori Nochi , Fumio Takaiwa , Hidenori Takagi , Lijyun Yang , Kazuya Suzuki , Hiroyasu Ebinuma , Koichi Sugita , Saori Kasahara
发明人： Yoshikazu Yuki , Hiroshi Kiyono , Takachika Hiroi , Tomonori Nochi , Fumio Takaiwa , Hidenori Takagi , Lijyun Yang , Kazuya Suzuki , Hiroyasu Ebinuma , Koichi Sugita , Saori Kasahara
IPC分类号： A01H5/00 , A61K39/00 , C12N15/82
CPC分类号： A61K39/08 , A23L7/196 , A23V2002/00 , A61K39/107 , A61K39/12 , A61K39/21 , C07K14/005 , C07K2319/55 , C12N15/8258 , C12N2740/16122 , C12N2740/16134 , A23V2300/21
摘要： To provide a transgenic rice plant that can produce rice which can be used as an “edible vaccine”, i.e., rice capable of inducing a desired immune response when mucosally administered such as an oral administration. There is provided a transgenic rice plant including a genomic DNA, wherein a DNA construct is incorporated into the genomic DNA so that the DNA construct is capable of being expressed, wherein the DNA construct includes a DNA encoding an antigenic protein, and a rice endosperm specific promoter linked upstream thereof.
摘要翻译：提供可以产生可以用作“可食用疫苗”的水稻的转基因水稻植物，即当粘膜给药如口服给药时能够诱导所需免疫应答的水稻。提供了包括基因组DNA的转基因水稻植物，其中将DNA构建体并入基因组DNA中，使得DNA构建体能够被表达，其中所述DNA构建体包括编码抗原蛋白的DNA和水稻胚乳特异性启动子连接上游。

6. 发明申请

US20110203016A1 PROCESS FOR PRODUCING PLANT STORAGE ORGAN WITH HIGH PRODUCTION OF RECOMBINANT PROTEIN AND NOVEL RECOMBINANT PROTEIN 审中-公开
标题翻译：生产重组蛋白和新型重组蛋白的植物储存制剂的生产工艺
公开(公告)号：US20110203016A1
公开(公告)日：2011-08-18
申请号：US12765711
申请日：2010-04-22
申请人： Koichi SUGITA , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa
发明人： Koichi SUGITA , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa
IPC分类号： A01H1/00 , A01H5/00 , C12N15/63
CPC分类号： C12N15/8257 , C07K14/605 , C12N15/8216
摘要： The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the cytokinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.
摘要翻译：本发明提供了在植物贮藏器官和GLP-1衍生物中高度产生重组蛋白的方法。通过使用包含重组蛋白基因，细胞分裂素相关基因，耐药性基因和可移除DNA元件的载体转化获得重组蛋白质高度产生的植物贮藏器官，其中细胞分裂素相关基因和耐药基因存在于可以与DNA元件一起表现的位置，而在植物储存器官中表达的重组蛋白存在于与DNA不一致的位置元件。通过使用该方法制备GLP-1，进一步提供了稳定的酶促消化的衍生物。

7. 发明申请

US20120054923A1 METHOD FOR CULTIVATION OF GENETICALLY-MODIFIED PLANT 有权
标题翻译：基因改造植物的培育方法
公开(公告)号：US20120054923A1
公开(公告)日：2012-03-01
申请号：US13147436
申请日：2009-08-26
申请人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
发明人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
IPC分类号： A01H5/10 , A01G31/00 , A01H5/00
CPC分类号： C12N15/8261 , C12N15/8234 , C12N15/8238 , C12N15/8251 , C12N15/8257 , Y02A40/146
摘要： The present invention provides a method for cultivation of a genetically-modified plant that can highly produce a desired protein. More specifically, the present invention provides a method for cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a medium, wherein the genetically-modified plant is transformed by introducing an expression vector comprising a promoter regulating expression of RNA and a seed storage protein isolated from a plant that highly expresses RNA and the seed storage protein under a high nitrogen condition; and a polynucleotide encoding an objective protein, and wherein the medium is adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a definite period in a period from 30 days before an expected flowering date to a date on or before flowering of the genetically-modified plant.
摘要翻译：本发明提供了能够高度产生所需蛋白质的遗传修饰植物的培养方法。更具体地说，本发明提供了一种遗传修饰植物的培养方法，包括：在培养基中培养经遗传修饰的植物，其中转基因修饰植物通过引入包含调节RNA表达的启动子的表达载体和在高氮条件下从高度表达RNA和种子储存蛋白的植物中分离的种子储存蛋白; 和编码目标蛋白质的多核苷酸，并且调节培养基使得硝酸盐氮在70mg / L至750mg / L和/或铵氮为70mg / L至750mg / L的条件下在一定期间内从预计开花日期前30天到转基因植物开花前后的日期。

8. 发明授权

US09353379B2 Method for cultivation of genetically-modified plant 有权
标题翻译：转基因植物栽培方法
公开(公告)号：US09353379B2
公开(公告)日：2016-05-31
申请号：US13147436
申请日：2009-08-26
申请人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
发明人： Saori Kasahara , Masafumi Wasai , Koichi Sugita , Teruhisa Shimada
IPC分类号： A01H1/00 , C12N15/82 , A01H5/00 , A01H5/10 , C12N15/00
CPC分类号： C12N15/8261 , C12N15/8234 , C12N15/8238 , C12N15/8251 , C12N15/8257 , Y02A40/146
摘要： The present invention provides a method for cultivation of a genetically-modified plant that can highly produce a desired protein. More specifically, the present invention provides a method for cultivation of a genetically-modified plant comprising: cultivating the genetically-modified plant in a medium, wherein the genetically-modified plant is transformed by introducing an expression vector comprising a promoter regulating expression of RNA and a seed storage protein isolated from a plant that highly expresses RNA and the seed storage protein under a high nitrogen condition; and a polynucleotide encoding an objective protein, and wherein the medium is adjusted so that nitrate nitrogen is 70 mg/L to 750 mg/L and/or ammonium nitrogen is 70 mg/L to 750 mg/L for a definite period in a period from 30 days before an expected flowering date to a date on or before flowering of the genetically-modified plant.
摘要翻译：本发明提供了能够高度产生所需蛋白质的遗传修饰植物的培养方法。更具体地说，本发明提供了一种遗传修饰植物的培养方法，包括：在培养基中培养经遗传修饰的植物，其中转基因修饰植物通过引入包含调节RNA表达的启动子的表达载体和在高氮条件下从高度表达RNA和种子储存蛋白的植物中分离的种子储存蛋白; 和编码目标蛋白质的多核苷酸，并且调节培养基使得硝酸盐氮在70mg / L至750mg / L和/或铵氮为70mg / L至750mg / L的条件下在一定期间内从预计开花日期前30天到转基因植物开花前后的日期。

9. 发明申请

US20120244176A1 PROTEIN HAVING IMMUNOGENICITY OF CEDAR POLLEN, POLYNUCLEOTIDE ENCODING THE PROTEIN, AND USE THEREOF 有权
标题翻译：蛋白质具有免疫原性的蛋白质，编码蛋白质的多核苷酸及其用途
公开(公告)号：US20120244176A1
公开(公告)日：2012-09-27
申请号：US13504636
申请日：2010-10-28
申请人： Fumio Takaiwa , Hidenori Takagi , Yuhya Wakasa , Yuji Fujii , Tomotaka Shinya , Saori Kasahara
发明人： Fumio Takaiwa , Hidenori Takagi , Yuhya Wakasa , Yuji Fujii , Tomotaka Shinya , Saori Kasahara
IPC分类号： A61K39/36 , C12N15/29 , A61P37/08 , A01H5/00 , C12P21/02 , C12N15/82 , C07K14/415 , C12N15/63
CPC分类号： C07K14/415 , A61K38/00 , A61K39/36
摘要： It is an object of the present invention to provide a protein that can successfully treats almost all patients with cedar pollinosis and can be ingested as a food. That is, the present invention provides a protein comprising an amino acid sequence represented by SEQ ID NO:1 or comprising an amino acid sequence having a mutation(s) in said amino acid sequence, and having an immunogenicity of a cedar pollen; a polynucleotide encoding said protein; a vector comprising said polynucleotide; a transformant comprising said polynucleotide or said vector; and intended uses of said protein, said polynucleotide, and said transformant.
摘要翻译：本发明的目的是提供一种可以成功地治疗几乎所有患有雪松花粉症的患者的蛋白质，并且可以作为食物摄取。也就是说，本发明提供了包含由SEQ ID NO：1所示的氨基酸序列或包含在所述氨基酸序列中具有突变并具有雪松花粉免疫原性的氨基酸序列的蛋白质的蛋白质。编码所述蛋白质的多核苷酸; 包含所述多核苷酸的载体; 包含所述多核苷酸或所述载体的转化体; 和所述蛋白质，所述多核苷酸和所述转化体的预期用途。

10. 发明授权

US09260491B2 Protein having immunogenicity of cedar pollen, polynucleotide encoding the protein, and use thereof 有权
标题翻译：具有雪松花粉免疫原性的蛋白质，编码蛋白质的多核苷酸及其用途
公开(公告)号：US09260491B2
公开(公告)日：2016-02-16
申请号：US13504636
申请日：2010-10-28
申请人： Fumio Takaiwa , Hidenori Takagi , Yuhya Wakasa , Yuji Fujii , Tomotaka Shinya , Saori Kasahara
发明人： Fumio Takaiwa , Hidenori Takagi , Yuhya Wakasa , Yuji Fujii , Tomotaka Shinya , Saori Kasahara
IPC分类号： A61K39/36 , C07K14/415 , A61K38/00
CPC分类号： C07K14/415 , A61K38/00 , A61K39/36
摘要： It is an object of the present invention to provide a protein that can successfully treats almost all patients with cedar pollinosis and can be ingested as a food. That is, the present invention provides a protein comprising an amino acid sequence represented by SEQ ID NO:1 or comprising an amino acid sequence having a mutation(s) in said amino acid sequence, and having an immunogenicity of a cedar pollen; a polynucleotide encoding said protein; a vector comprising said polynucleotide; a transformant comprising said polynucleotide or said vector; and intended uses of said protein, said polynucleotide, and said transformant.
摘要翻译：本发明的目的是提供一种可以成功地治疗几乎所有患有雪松花粉症的患者的蛋白质，并且可以作为食物摄取。也就是说，本发明提供了包含由SEQ ID NO：1所示的氨基酸序列或包含在所述氨基酸序列中具有突变并具有雪松花粉免疫原性的氨基酸序列的蛋白质的蛋白质。编码所述蛋白质的多核苷酸; 包含所述多核苷酸的载体; 包含所述多核苷酸或所述载体的转化体; 和所述蛋白质，所述多核苷酸和所述转化体的预期用途。

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