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    • 1. 发明授权
    • Method of multiquantification for cholesterol of low-density lipoprotein
    • 低密度脂蛋白胆固醇多量化方法
    • US07811779B2
    • 2010-10-12
    • US10594898
    • 2005-03-30
    • Keiko MatsumotoHiroshi Matsui
    • Keiko MatsumotoHiroshi Matsui
    • C12Q1/60C12Q1/44C12Q1/28
    • C12Q1/28C12Q1/60
    • Provided is a method of stabilizing a reagent that allows simultaneous quantification of LDL cholesterol and total cholesterol by a single measurement by suppressing spontaneous color development thereof. A method of quantification for cholesterol in low density lipoprotein and total cholesterol in a biological sample by the single measurement comprises a first step of treating lipoproteins other than low density lipoprotein in the biological sample to generate hydrogen peroxide and a second step of converting the hydrogen peroxide obtained in the first step to a quinone dye and treating remaining low density lipoprotein and converting generated hydrogen peroxide to the quinone dye, where the quinone dye is not formed in the first step, and cholesterol in low density lipoprotein and total cholesterol are quantified from the amount of the quinone dye formed in the second step by the single measurement.
    • 提供一种稳定试剂的方法,其通过抑制其自发发色来通过单次测量同时定量LDL胆固醇和总胆固醇。 通过单次测量对生物样品中的低密度脂蛋白和总胆固醇的胆固醇进行定量的方法包括在生物样品中处理低密度脂蛋白以外的脂蛋白以产生过氧化氢的第一步骤,以及转化过氧化氢 在第一步中得到醌染料并处理剩余的低密度脂蛋白,并将产生的过氧化氢转化为醌染料,其中在第一步中不形成醌染料,并且从低密度脂蛋白和总胆固醇中的胆固醇从 通过单次测量在第二步中形成的醌染料的量。
    • 3. 发明申请
    • Method Of Multiquantification For Cholesterol Of Low-Density Lipoprotein
    • 低密度脂蛋白胆固醇多功能化方法
    • US20070207515A1
    • 2007-09-06
    • US10594898
    • 2005-03-30
    • Keiko MatsumotoHiroshi Matsui
    • Keiko MatsumotoHiroshi Matsui
    • C12Q1/60G01N33/92
    • C12Q1/28C12Q1/60
    • Provided is a method of stabilizing a reagent that allows simultaneous quantification of LDL cholesterol and total cholesterol by a single measurement by suppressing spontaneous color development thereof A method of quantification for cholesterol in low density lipoprotein and total cholesterol in a biological sample by the single measurement comprises a first step of treating lipoproteins other than low density lipoprotein in the biological sample to generate hydrogen peroxide and a second step of converting the hydrogen peroxide obtained in the first step to a quinone dye and treating remaining low density lipoprotein and converting generated hydrogen peroxide to the quinone dye, where the quinone dye is not formed in the first step, and cholesterol in low density lipoprotein and total cholesterol are quantified from the amount of the quinone dye formed in the second step by the single measurement.
    • 提供了通过抑制自发发色来通过单次测量同时定量LDL胆固醇和总胆固醇的方法。通过单次测量在生物样品中定量低密度脂蛋白中的胆固醇和总胆固醇的方法包括 在生物样品中处理除了低密度脂蛋白之外的脂蛋白以产生过氧化氢的第一步骤,以及将第一步中获得的过氧化氢转化为醌染料并处理剩余的低密度脂蛋白并将产生的过氧化氢转化为 醌染料,其中在第一步中不形成醌染料,并且通过单次测量由第二步中形成的醌染料的量定量低密度脂蛋白和总胆固醇中的胆固醇。