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1. 发明授权

US08119360B2 Method, reagent and kit for determination of cholesterol in remnant-like particles (RLP) 有权
标题翻译：用于测定残留样颗粒（RLP）中胆固醇的方法，试剂和试剂盒
公开(公告)号：US08119360B2
公开(公告)日：2012-02-21
申请号：US11815225
申请日：2006-02-14
申请人： Kazuhito Miyauchi , Mayumi Fujinaka
发明人： Kazuhito Miyauchi , Mayumi Fujinaka
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60 , C12Q1/26 , C12Q1/32 , C12Q1/44 , G01N33/92
摘要： The present invention provides a method, a reagent and a kit for simple and sensitive determination of cholesterol in remnant-like particles without separation of components of a sample. A method for quantitatively determining remnant-like particle cholesterol in a sample, which comprises: in an aqueous medium containing the sample and in the presence of a combination of specific surfactants and a phospholipid-hydrolyzing enzyme, allowing remnant-like particle cholesterol in the sample to react with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase (in the presence of oxidized coenzyme); and determining the formed hydrogen peroxide or reduced coenzyme.
摘要翻译：本发明提供了用于简单且灵敏地测定残留样颗粒中胆固醇而不分离样品组分的方法，试剂和试剂盒。一种用于定量测定样品中残留样颗粒胆固醇的方法，其包括：在含有样品的水性介质中，并且在特定表面活性剂和磷脂水解酶的组合存在下，允许样品中的残留物颗粒胆固醇与胆固醇酯酶和胆固醇氧化酶或胆固醇脱氢酶（在氧化型辅酶存在下）反应; 并测定形成的过氧化氢或还原型辅酶。

2. 发明申请

US20090023167A1 METHOD, REAGENT AND KIT FOR DETERMINATION OF CHOLESTEROL IN REMNANT-LIKE PARTICLES (RLP) 有权
标题翻译：方法，试剂和试剂盒，用于确定残留样颗粒中的胆固醇（RLP）
公开(公告)号：US20090023167A1
公开(公告)日：2009-01-22
申请号：US11815225
申请日：2006-02-14
申请人： Kazuhito Miyauchi , Mayumi Fujinaka
发明人： Kazuhito Miyauchi , Mayumi Fujinaka
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60 , C12Q1/26 , C12Q1/32 , C12Q1/44 , G01N33/92
摘要： The present invention provides a method, a reagent and a kit for simple and sensitive determination of cholesterol in remnant-like particles without separation of components of a sample. A method for quantitatively determining remnant-like particle cholesterol in a sample, which comprises: in an aqueous medium containing the sample and in the presence of a combination of specific surfactants and a phospholipid-hydrolyzing enzyme, allowing remnant-like particle cholesterol in the sample to react with cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase (in the presence of oxidized coenzyme); and determining the formed hydrogen peroxide or reduced coenzyme.
摘要翻译：本发明提供了用于简单且灵敏地测定残留样颗粒中胆固醇而不分离样品组分的方法，试剂和试剂盒。一种用于定量测定样品中残留样颗粒胆固醇的方法，其包括：在含有样品的水性介质中，并且在特定表面活性剂和磷脂水解酶的组合存在下，允许样品中的残留物颗粒胆固醇与胆固醇酯酶和胆固醇氧化酶或胆固醇脱氢酶（在氧化型辅酶存在下）反应; 并测定形成的过氧化氢或还原型辅酶。

3. 发明授权

US09051600B2 Sphingomyelin measurement method using sequential phospholipase D reactions 有权
标题翻译：鞘磷脂测定方法采用顺序磷脂酶D反应
公开(公告)号：US09051600B2
公开(公告)日：2015-06-09
申请号：US14232427
申请日：2012-07-25
申请人： Takehide Kimura , Kazuhito Miyauchi , Hideyuki Kuwata
发明人： Takehide Kimura , Kazuhito Miyauchi , Hideyuki Kuwata
IPC分类号： C12Q1/44 , C12Q1/61 , G01N33/92 , C12Q1/28
CPC分类号： C12Q1/61 , C12Q1/28 , C12Q1/44 , C12Y101/03017 , C12Y301/01005 , C12Y301/04004 , G01N33/92 , G01N2333/92 , G01N2405/08
摘要： Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefore. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.
摘要翻译：本发明提供了简单且准确地测量样品中鞘磷脂的方法，因此提供了一种试剂盒。该方法是测量样品中鞘磷脂的方法，包括使样品与磷脂酶D反应，磷脂酶D不与鞘磷脂和溶血磷脂酰胆碱反应，但与磷脂酰胆碱，溶血磷脂酶或单甘油脂肪酶和胆碱氧化酶反应，消除形成的过氧化氢使所得物与不与甘油-3-磷酸胆碱和游离脂肪酸反应但与鞘磷脂反应的磷脂酶D和胆碱氧化酶，并测量形成的过氧化氢。

4. 发明申请

US20140162300A1 SPHINGOMYELIN MEASUREMENT METHOD AND MEASUREMENT KIT 有权
标题翻译： SPHINGOMYELIN测量方法和测量套件
公开(公告)号：US20140162300A1
公开(公告)日：2014-06-12
申请号：US14232427
申请日：2012-07-25
申请人： Takehide Kimura , Kazuhito Miyauchi , Hideyuki Kuwata
发明人： Takehide Kimura , Kazuhito Miyauchi , Hideyuki Kuwata
IPC分类号： C12Q1/61
CPC分类号： C12Q1/61 , C12Q1/28 , C12Q1/44 , C12Y101/03017 , C12Y301/01005 , C12Y301/04004 , G01N33/92 , G01N2333/92 , G01N2405/08
摘要： Provided is a method for simply and accurately measuring sphingomyelin in a sample, and a kit therefor. The method is a method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine, a lysophospholipase or a monoglyceride lipase, and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin, and a choline oxidase, and measuring the formed hydrogen peroxide.
摘要翻译：本发明提供了简单且准确地测定样品中鞘磷脂的方法及其用途。该方法是测量样品中鞘磷脂的方法，包括使样品与磷脂酶D反应，磷脂酶D不与鞘磷脂和溶血磷脂酰胆碱反应，但与磷脂酰胆碱，溶血磷脂酶或单甘油脂肪酶和胆碱氧化酶反应，消除形成的过氧化氢使所得物与不与甘油-3-磷酸胆碱和游离脂肪酸反应但与鞘磷脂反应的磷脂酶D和胆碱氧化酶，并测量形成的过氧化氢。

5. 发明授权

US07202047B2 Aqueous method for determining cholesterol in remnant-like particles using cholesterol esterase, phospholipase, surfactant and cholesterol oxidase or dehydrogenase 有权
标题翻译：使用胆固醇酯酶，磷脂酶，表面活性剂和胆固醇氧化酶或脱氢酶测定残留样颗粒中的胆固醇的水性方法
公开(公告)号：US07202047B2
公开(公告)日：2007-04-10
申请号：US10430231
申请日：2003-05-06
申请人： Kazuhito Miyauchi
发明人： Kazuhito Miyauchi
IPC分类号： C12Q1/60
CPC分类号： G01N33/92 , C12Q1/60
摘要： The present invention provides a simple and sensitive aqueous method for determining cholesterol in remnant-like particles in a biological sample without the separation of components of the sample. The method comprises contacting the sample with (i) cholesterol esterase, (ii) cholesterol oxidase or cholesterol dehydrogenase, and (iii) phospholipase with a surfactant that inhibits cholesterol oxidase or cholesterol dehydrogenase in lipoproteins other than remnant-like particles in the presence of oxygen or an oxidized coenzyme. Formed hydrogen peroxide or reduced coenzyme is measured and correlated to a quantitative determination of cholesterol in remnant-like particles.
摘要翻译：本发明提供了用于在不分离样品的组分的情况下测定生物样品中残留样颗粒中的胆固醇的简单且灵敏的水性方法。该方法包括使样品与（i）胆固醇酯酶，（ii）胆固醇氧化酶或胆固醇脱氢酶，和（iii）磷脂酶与在氧气存在下除残留样颗粒之外的脂蛋白中抑制胆固醇氧化酶或胆固醇脱氢酶的表面活性剂接触或氧化型辅酶。测量形成的过氧化氢或还原型辅酶，并与残留样颗粒中胆固醇的定量测定相关。

6. 发明申请

US20050042703A1 Method for quantitating triglycerides in lipoproteins 审中-公开
标题翻译：脂蛋白中甘油三酯的定量方法
公开(公告)号：US20050042703A1
公开(公告)日：2005-02-24
申请号：US10951683
申请日：2004-09-29
申请人： Kazuhito Miyauchi , Shizuyo Takada , Tomomi Murakami , Akira Miike
发明人： Kazuhito Miyauchi , Shizuyo Takada , Tomomi Murakami , Akira Miike
IPC分类号： C12Q1/44 , C12Q1/61 , C12Q1/60 , C12Q1/26
CPC分类号： C12Q1/61 , C12Q1/44 , G01N2333/904 , G01N2333/908 , G01N2333/918
摘要： The present invention relates to a method for conveniently quantitating TGs contained in various lipoproteins. A method for quantitating trigyceride (TG) in a particular lipoprotein which comprises eliminating free glycerol from a sample containing free glycerol and TG in the particular lipoprotein, then allowing the resulting sample to react with lipoprotein lipase and an enzyme system which generates hydrogen peroxide from free glycerol, and quantitating the generated hydrogen peroxide, is provided.
摘要翻译：本发明涉及方便地定量各种脂蛋白中含有的TG的方法。一种在特定脂蛋白中定量三环霉素（TG）的方法，其包括从含有游离甘油和特定脂蛋白中的TG的样品中除去游离甘油，然后使得到的样品与脂蛋白脂肪酶和从游离体产生过氧化氢的酶系统反应甘油，并定量生成的过氧化氢。

7. 发明授权

US5858695A Method of quantitative determination of bilirubin and a reagent therefor 失效
标题翻译：定量测定胆红素及其试剂的方法
公开(公告)号：US5858695A
公开(公告)日：1999-01-12
申请号：US736058
申请日：1996-10-22
申请人： Akira Kadota , Kayoko Shigenobu , Akira Miike , Kazuhito Miyauchi
发明人： Akira Kadota , Kayoko Shigenobu , Akira Miike , Kazuhito Miyauchi
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26
摘要： Disclosed are a method for quantitative determination of bilirubin and a reagent for the method, which are useful in clinical examinations. The method comprises subjecting the bilirubin in a sample to coexist with ascorbate oxidase and a reaction promoter in aqueous medium to thereby oxidize the bilirubin, measuring the change in absorbance of the aqueous medium and comparing the change in absorbance with a calibration curve. The reagent comprises ascorbate oxidase and a reaction promoter.
摘要翻译：公开了用于定量测定胆红素和该方法的试剂的方法，其可用于临床检查。该方法包括使样品中的胆红素与水性介质中的抗坏血酸氧化酶和反应促进剂共存，从而氧化胆红素，测量水性介质的吸光度变化，并将吸光度的变化与校准曲线进行比较。试剂包含抗坏血酸氧化酶和反应促进剂。

8. 发明授权

US5736406A Method of determining the amount of cholesterol in a high-density lipoprotein 失效
标题翻译：确定高密度脂蛋白中胆固醇含量的方法
公开(公告)号：US5736406A
公开(公告)日：1998-04-07
申请号：US545725
申请日：1995-11-02
申请人： Kazuhito Miyauchi , Norihiko Kayahara , Toshio Tatano , Eiko Shutoh , Hiroyuki Sugiuchi , Tetsumi Irie , Kaneto Uekama , Susumu Ohsawa
发明人： Kazuhito Miyauchi , Norihiko Kayahara , Toshio Tatano , Eiko Shutoh , Hiroyuki Sugiuchi , Tetsumi Irie , Kaneto Uekama , Susumu Ohsawa
IPC分类号： C12Q1/26 , C12Q1/28 , C12Q1/44 , C12Q1/60 , G01N21/31 , G01N21/77 , G01N33/92 , G01N33/50
CPC分类号： C12Q1/60 , G01N33/92
摘要： Provided are a method of determining the amount of cholesterol in high-density lipoprotein (HDL), which comprises measuring the amount of cholesterol in low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL) and chylomicron (CM) in a sample in the presence of a sugar compound and/or a protein solubilizing agent, and calculating the difference between the amount of cholesterol in LDL, VLDL and CM and the total amount of cholesterol in the sample, and a method of determining the amount of cholesterol in HDL, which comprises measuring the amount of cholesterol in HDL in a sample in the presence of a sugar compound and/or a protein solubilizing agent.
摘要翻译： PCT No.PCT / JP95 / 00379 Sec。 371日期：1995年11月2日 102（e）1995年11月2日日期PCT 1995年3月8日PCT PCT。出版物WO95 / 24647 日期1995年9月14日提供了一种测定高密度脂蛋白（HDL）胆固醇含量的方法，包括测定低密度脂蛋白（LDL），极低密度脂蛋白（VLDL）和乳糖微粒（CM）在糖化合物和/或蛋白质增溶剂存在下的样品中，并计算LDL，VLDL和CM中的胆固醇量与样品中胆固醇总量之间的差异，以及方法测定HDL中胆固醇的含量，其包括在糖化合物和/或蛋白质增溶剂的存在下测量样品中HDL中胆固醇的量。

9. 发明申请

US20090170139A1 METHOD, REAGENT AND KIT FOR MEASUREMENT OF CHOLESTEROL IN REMNANT-LIKE PARTICLES 审中-公开
标题翻译：方法，试剂和试剂盒，用于测定残留颗粒中的胆固醇
公开(公告)号：US20090170139A1
公开(公告)日：2009-07-02
申请号：US12095331
申请日：2006-12-08
申请人： Shingo Mishima , Kazuhito Miyauchi
发明人： Shingo Mishima , Kazuhito Miyauchi
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60 , G01N33/92 , G01N2333/904
摘要： The present invention provides a method for simple and accurate measurement of cholesterol in remnant-like lipoprotein (RLP) (RLP-C) in a sample, which comprises the steps of: (i) eliminating cholesterol in lipoproteins other than RLP by reacting the sample with cholesterol oxidase, or cholesterol dehydrogenase and oxidized coenzyme, in an aqueous medium comprising surfactant A; (ii) reacting the reaction solution from which cholesterol in lipoproteins other than RLP has been eliminated in the above step (i) with cholesterol esterase and cholesterol oxidase, or cholesterol esterase, cholesterol dehydrogenase and oxidized coenzyme, in the presence of surfactant B to form hydrogen peroxide or reduced coenzyme; and (iii) measuring the hydrogen peroxide or reduced coenzyme formed in the above step (ii).
摘要翻译：本发明提供了一种用于简单且精确地测量样品中残留样脂蛋白（RLP）（RLP-C）中的胆固醇的方法，其包括以下步骤：（i）通过使样品反应来消除除RLP之外的脂蛋白中的胆固醇胆固醇氧化酶或胆固醇脱氢酶和氧化的辅酶，在包含表面活性剂A的水性介质中; （ii）在表面活性剂B存在下，使上述步骤（i）中的胆固醇在胆固醇酯酶和胆固醇氧化酶或胆固醇酯酶，胆固醇脱氢酶和氧化型辅酶之外已经消除了除了RLP之外的脂蛋白中的胆固醇的反应溶液以形成过氧化氢或还原型辅酶; 和（iii）测量在上述步骤（ii）中形成的过氧化氢或还原型辅酶。

10. 发明申请

US20070161068A1 Method, reagent and kit for determination of cholesterol in very low-density lipoprotein remnant (vldl remnant) 审中-公开
标题翻译：用于测定非常低密度脂蛋白残留物（vldl残留物）中胆固醇的方法，试剂和试剂盒
公开(公告)号：US20070161068A1
公开(公告)日：2007-07-12
申请号：US10586868
申请日：2005-01-28
申请人： Kazuhito Miyauchi
发明人： Kazuhito Miyauchi
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60 , C12Q1/34 , G01N33/92 , G01N2800/044
摘要： According to the present invention, esterified and free cholesterol in very low-density lipoprotein remnant (hereinafter collectively referred to as very low-density lipoprotein remnant cholesterol) in a sample is determined in an aqueous medium containing the sample in the presence of a combination of (a) a polyoxyethylene-polyoxyalkylene alkylaryl ether and (b) one kind of surfactant selected from the group consisting of a polyoxyethylene-polyoxybutylene copolymer, a polyoxyethylene styrenated-phenyl ether and a polyoxyalkylene long-chain alkyl ether which combination is capable of causing cholesterol esterase and cholesterol oxidase or cholesterol dehydrogenase to act specifically on very low-density lipoprotein remnant cholesterol.
摘要翻译：根据本发明，在包含样品的水性介质中，在存在以下组合的情况下，测定样品中极低密度脂蛋白残留物（以下统称为极低密度脂蛋白残留胆固醇）中的酯化游离胆固醇（a）聚氧乙烯 - 聚氧化烯烷基芳基醚和（b）一种选自聚氧乙烯 - 聚氧丁烯共聚物，聚氧乙烯苯乙烯基苯基醚和聚氧化烯长链烷基醚的表面活性剂，其组合能够引起胆固醇酯酶和胆固醇氧化酶或胆固醇脱氢酶作用于非常低密度脂蛋白残留胆固醇。

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