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1. 发明申请

US20050042703A1 Method for quantitating triglycerides in lipoproteins 审中-公开
标题翻译：脂蛋白中甘油三酯的定量方法
公开(公告)号：US20050042703A1
公开(公告)日：2005-02-24
申请号：US10951683
申请日：2004-09-29
申请人： Kazuhito Miyauchi , Shizuyo Takada , Tomomi Murakami , Akira Miike
发明人： Kazuhito Miyauchi , Shizuyo Takada , Tomomi Murakami , Akira Miike
IPC分类号： C12Q1/44 , C12Q1/61 , C12Q1/60 , C12Q1/26
CPC分类号： C12Q1/61 , C12Q1/44 , G01N2333/904 , G01N2333/908 , G01N2333/918
摘要： The present invention relates to a method for conveniently quantitating TGs contained in various lipoproteins. A method for quantitating trigyceride (TG) in a particular lipoprotein which comprises eliminating free glycerol from a sample containing free glycerol and TG in the particular lipoprotein, then allowing the resulting sample to react with lipoprotein lipase and an enzyme system which generates hydrogen peroxide from free glycerol, and quantitating the generated hydrogen peroxide, is provided.
摘要翻译：本发明涉及方便地定量各种脂蛋白中含有的TG的方法。一种在特定脂蛋白中定量三环霉素（TG）的方法，其包括从含有游离甘油和特定脂蛋白中的TG的样品中除去游离甘油，然后使得到的样品与脂蛋白脂肪酶和从游离体产生过氧化氢的酶系统反应甘油，并定量生成的过氧化氢。

2. 发明授权

US06811994B1 Method for quantitating triglycerides in lipoproteins 有权
标题翻译：脂蛋白中甘油三酯的定量方法
公开(公告)号：US06811994B1
公开(公告)日：2004-11-02
申请号：US09889742
申请日：2001-07-20
申请人： Kazuhito Miyauchi , Shizuyo Takada , Tomomi Murakami , Akira Miike
发明人： Kazuhito Miyauchi , Shizuyo Takada , Tomomi Murakami , Akira Miike
IPC分类号： C12Q134
CPC分类号： C12Q1/61 , C12Q1/44 , G01N2333/904 , G01N2333/908 , G01N2333/918
摘要： The present invention relates to a method for conveniently quantitating TGs contained in various lipoproteins. A method for quantitating trigyceride (TG) in a particular lipoprotein which comprises eliminating free glycerol from a sample containing free glycerol and TG in the particular lipoprotein, then allowing the resulting sample to react with lipoprotein lipase and an enzyme system which generates hydrogen peroxide from free glycerol, and quantitating the generated hydrogen peroxide, is provided.
摘要翻译：本发明涉及方便地定量各种脂蛋白中含有的TG的方法。一种在特定脂蛋白中定量三环霉素（TG）的方法，其包括从含有游离甘油和特定脂蛋白中的TG的样品中除去游离甘油，然后使得到的样品与脂蛋白脂肪酶和从游离体产生过氧化氢的酶系统反应甘油，并定量生成的过氧化氢。

3. 发明授权

US09360432B2 Method for measuring triglyceride in low-density lipoprotein 有权
标题翻译：在低密度脂蛋白中测量甘油三酯的方法
公开(公告)号：US09360432B2
公开(公告)日：2016-06-07
申请号：US12090906
申请日：2006-10-31
申请人： Yuki Katayama , Kazuhito Miyauchi , Shizuyo Takada , Tomomi Murakami
发明人： Yuki Katayama , Kazuhito Miyauchi , Shizuyo Takada , Tomomi Murakami
IPC分类号： G01N21/78 , G01N33/92 , C12Q1/44 , C12Q1/61
CPC分类号： G01N21/78 , C12Q1/44 , C12Q1/61 , G01N33/92 , G01N2333/91215 , G01N2333/918
摘要： The present invention provides a method for a simple and accurate measurement of triglycerides in low-density lipoprotein in a sample comprising performing the following steps sequentially:(i) a step of generating free glycerol by allowing lipoprotein lipase to act on a sample, in an aqueous medium comprising the sample and a specific surfactant such as polyoxyethylene polyoxyalkylene polycyclic phenyl ether; (ii) a step of removing free glycerol present in the reaction solution of the above step (i); (iii) a step of generating free glycerol by allowing lipoprotein lipase to act on the reaction solution from which free glycerol has been removed in step (ii), in the presence of a specific surfactant such as polyoxyethylene polyoxyalkylene alkyl ether; and (iv) a step of measuring free glycerol generated in step (iii), and a kit used for the method.
摘要翻译：本发明提供了一种用于简单且准确地测量样品中低密度脂蛋白中的甘油三酸酯的方法，包括依次执行以下步骤：（i）通过使脂蛋白脂肪酶作用于样品，产生游离甘油的步骤，包含样品的水性介质和特定表面活性剂如聚氧乙烯聚氧化烯多环苯基醚; （ii）除去上述步骤（i）的反应溶液中存在的游离甘油的步骤; （iii）在特定表面活性剂如聚氧乙烯聚氧化烯烷基醚的存在下，通过使脂蛋白脂肪酶作用于在步骤（ii）中除去游离甘油的反应溶液中，产生游离甘油的步骤; 和（iv）测量步骤（iii）中产生的游离甘油的步骤和用于该方法的试剂盒。

4. 发明申请

US20090226944A1 METHOD FOR MEASURING TRIGLYCERIDE IN LOW-DENSITY LIPOPROTEIN AND KIT FOR MEASUREMENT 有权
标题翻译：用于测量低密度脂蛋白中的TRIGLYCERIDE的方法和用于测量的试剂盒
公开(公告)号：US20090226944A1
公开(公告)日：2009-09-10
申请号：US12090906
申请日：2006-10-31
申请人： Yuki Katayama , Kazuhito Miyauchi , Shizuyo Takada , Tomomi Murakami
发明人： Yuki Katayama , Kazuhito Miyauchi , Shizuyo Takada , Tomomi Murakami
IPC分类号： C12Q1/60
CPC分类号： G01N21/78 , C12Q1/44 , C12Q1/61 , G01N33/92 , G01N2333/91215 , G01N2333/918
摘要： The present invention provides a method for a simple and accurate measurement of triglycerides in low-density lipoprotein in a sample comprising performing the following steps sequentially:(i) a step of generating free glycerol by allowing lipoprotein lipase to act on a sample, in an aqueous medium comprising the sample and a specific surfactant such as polyoxyethylene polyoxyalkylene polycyclic phenyl ether; (ii) a step of removing free glycerol present in the reaction solution of the above step (i); (iii) a step of generating free glycerol by allowing lipoprotein lipase to act on the reaction solution from which free glycerol has been removed in step (ii), in the presence of a specific surfactant such as polyoxyethylene polyoxyalkylene alkyl ether; and (iv) a step of measuring free glycerol generated in step (iii), and a kit used for the method.
摘要翻译：本发明提供了一种用于简单且准确地测量样品中低密度脂蛋白中的甘油三酸酯的方法，包括依次执行以下步骤：（i）通过使脂蛋白脂肪酶作用于样品，产生游离甘油的步骤，包含样品的水性介质和特定表面活性剂如聚氧乙烯聚氧化烯多环苯基醚; （ii）除去上述步骤（i）的反应溶液中存在的游离甘油的步骤; （iii）在特定表面活性剂如聚氧乙烯聚氧化烯烷基醚的存在下，通过使脂蛋白脂肪酶作用于在步骤（ii）中除去游离甘油的反应溶液中，产生游离甘油的步骤; 和（iv）测量步骤（iii）中产生的游离甘油的步骤和用于该方法的试剂盒。

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