专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20090286330A1 Immunoassay using insoluble carrier particles and reagent therefor 有权
标题翻译：免疫测定使用不溶性载体颗粒及其试剂
公开(公告)号：US20090286330A1
公开(公告)日：2009-11-19
申请号：US12509895
申请日：2009-07-27
申请人： Kayoko Shigenobu
发明人： Kayoko Shigenobu
IPC分类号： G01N33/544
CPC分类号： G01N33/5306 , G01N33/54313
摘要： The present invention provides a reagent for an immunoassay comprising insoluble carrier particles which can give the values to be determined with high accuracy and reliability, and can be stored for a long time; an immunoassay using the reagent; and a method for keeping the reagent stable. The present invention provides an immunoassay which comprises carrying out an antigen-antibody reaction using insoluble carrier particles in an aqueous medium comprising a buffer having its buffer capacity in a neutral or alkaline region (except for a carbonate-based buffer) and a carbonic acid compound releasing a bicarbonate ion; a reagent for an immunoassay comprising a buffer having its buffer capacity in a neutral or alkaline region (except for a carbonate-based buffer), a carbonic acid compound releasing bicarbonate ion, and insoluble carrier particles; and a method for keeping the reagent for an immunoassay comprising insoluble carrier particles stable, which comprises allowing insoluble carrier particles to co-exist in an aqueous medium comprising a buffer having its buffer capacity in a neutral or alkaline region (except for a carbonate-based buffer) and a carbonic acid compound releasing a bicarbonate ion.
摘要翻译：本发明提供了一种免疫测定用试剂，其包含不溶性载体颗粒，其可以以高精度和可靠性给出待测定的值，并且可以长时间储存; 使用试剂进行免疫测定; 和保持试剂稳定的方法。本发明提供了一种免疫测定法，其包括使用不溶性载体颗粒在水性介质中进行抗原 - 抗体反应，所述水性介质包含在中性或碱性区域（除了碳酸盐基缓冲液）之外具有缓冲能力的缓冲液和碳酸化合物释放碳酸氢根离子; 用于免疫测定的试剂，其包含在中性或碱性区域（除了碳酸酯类缓冲液）之外具有缓冲能力的缓冲液，释放碳酸氢根离子的碳酸化合物和不溶性载体颗粒; 以及用于保持包含不溶性载体颗粒的免疫测定试剂稳定的方法，其包括使不溶性载体颗粒共存于包含其在中性或碱性区域中具有缓冲能力的缓冲液的水性介质（除了碳酸酯基缓冲液）和释放碳酸氢根离子的碳酸化合物。

2. 发明授权

US5766870A Method of quantitative determination of sodium ions 失效
标题翻译：钠离子定量测定方法
公开(公告)号：US5766870A
公开(公告)日：1998-06-16
申请号：US601031
申请日：1996-05-03
申请人： Kayoko Shigenobu , Norihito Aoyama
发明人： Kayoko Shigenobu , Norihito Aoyama
IPC分类号： C12Q1/34 , C12Q1/54 , G01N33/84 , G01N33/53
CPC分类号： G01N33/84 , C12Q1/34 , C12Q1/54 , G01N2333/924 , Y10S435/962
摘要： The present invention relates to a method of quantitative determination of sodium ions in a sample using .beta.-galactosidase, wherein the .beta.-galactosidase reaction is conducted in the presence of at least one chelating agent selected from of 1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid, ethylenediamine-tetraacetic acid, triethylenetetranine-hexaacetic acid, diethylenetriamine-N,N,N',N",N"-pentaacetic acid, 1,3 -diminopropan-2-ol-N,N',N'-tetraacetic acid, ethylenediamine-N,N'-dipropionic acid dihydrochloride, ethylenediamine-tetrakis(methylenesulfonic acid), iminodiacetic acid, hydroxyimino-diacetic acid and nitrilotriacetic acid. The method of quantitative determination of sodium ions of the present invention is useful in clinical examinations and the accuracy in the measurement by the method is high.
摘要翻译： PCT No.PCT / JP94 / 01384 Sec。 371日期：1996年5月3日 102（e）日期1996年5月3日PCT 1994年8月22日PCT公布。公开号WO95 / 06135 日期1995年3月2日本发明涉及一种使用β-半乳糖苷酶定量测定样品中钠离子的方法，其中β-半乳糖苷酶反应在至少一种螯合剂存在下进行，所述螯合剂选自1,2- 环己烷二胺-N，N，N'，N'-四乙酸，乙二胺四乙酸，三亚乙基四胺六乙酸，二亚乙基三胺-N，N，N'，N“，N” - 五乙酸，1,3-二亚丙基 -2-羟基-N，N'，N'-四乙酸，乙二胺-N，N'-二丙酸二盐酸盐，乙二胺四（甲基磺酸），亚氨基二乙酸，羟基亚氨基二乙酸和次氮基三乙酸。本发明的钠离子的定量测定方法可用于临床检查，该方法的测定精度高。

3. 发明授权

US08431415B2 Immunoassay using insoluble carrier particles and reagent therefor 有权
公开(公告)号：US08431415B2
公开(公告)日：2013-04-30
申请号：US12509895
申请日：2009-07-27
申请人： Kayoko Shigenobu
发明人： Kayoko Shigenobu
IPC分类号： G01N33/546
CPC分类号： G01N33/5306 , G01N33/54313
摘要： The present invention provides a reagent for an immunoassay comprising insoluble carrier particles which can give the values to be determined with high accuracy and reliability, and can be stored for a long time; an immunoassay using the reagent; and a method for keeping the reagent stable. The present invention provides an immunoassay which comprises carrying out an antigen-antibody reaction using insoluble carrier particles in an aqueous medium comprising a buffer having its buffer capacity in a neutral or alkaline region (except for a carbonate-based buffer) and a carbonic acid compound releasing a bicarbonate ion; a reagent for an immunoassay comprising a buffer having its buffer capacity in a neutral or alkaline region (except for a carbonate-based buffer), a carbonic acid compound releasing bicarbonate ion, and insoluble carrier particles; and a method for keeping the reagent for an immunoassay comprising insoluble carrier particles stable, which comprises allowing insoluble carrier particles to co-exist in an aqueous medium comprising a buffer having its buffer capacity in a neutral or alkaline region (except for a carbonate-based buffer) and a carbonic acid compound releasing a bicarbonate ion.

4. 发明授权

US07166476B2 Highly reproducible agglutination immunoassay method and reagents 有权
标题翻译：高度可重复的凝集免疫测定法和试剂
公开(公告)号：US07166476B2
公开(公告)日：2007-01-23
申请号：US10363038
申请日：2001-08-28
申请人： Kayoko Shigenobu , Kenshiro Shuto , Shujiro Sakaki
发明人： Kayoko Shigenobu , Kenshiro Shuto , Shujiro Sakaki
IPC分类号： G01N33/536 , G01N33/546 , G01N33/53 , G01N33/539 , C08F130/02 , C07F9/09
CPC分类号： C08F220/60 , C08F230/02 , G01N33/54313 , G01N33/545 , G01N33/721 , Y10S435/81 , Y10S435/961
摘要： The present invention provides an agglutination immunoassay, wherein the agglutination of insoluble carrier particles such as latex are stabilized and uniformized to give good reproducibility, and a reagent therefor. In the agglutination immunoassay which comprises allowing an antigenic substance in a sample to bind to insoluble carrier particles carrying substantially neither antigens nor antibodies thereon, and allowing an antibody or an antibody complex which reacts specifically to the antigenic substance to bind to the antigenic substance to give a selective agglutination of the insoluble carrier particles, a homopolymer prepared by polymerization of a monomer such as 2-methacryloyloxyethyl phosphorylcholine having a phosphorylcholine group and a vinyl group, or a copolymer prepared by polymerization of a monomer having a phosphorylcholine group and a vinyl group, with a monomer having a vinyl group such as n-butyl methacrylate is used.
摘要翻译：本发明提供凝集免疫测定法，其中不溶性载体颗粒如胶乳的凝集是稳定和均匀的，以提供良好的再现性，以及其试剂。在凝集免疫测定中，包括使样品中的抗原性物质与基本上既不携带抗原也不携带抗体的不溶性载体颗粒结合，并且使与抗原物质特异性反应的抗体或抗体复合物与抗原物质结合，得到不溶性载体颗粒的选择性凝集，通过具有磷酸胆碱基和乙烯基的2-甲基丙烯酰氧基乙基磷酰胆碱等单体的聚合制备的均聚物或通过具有磷酸胆碱基和乙烯基的单体的聚合制备的共聚物，使用具有乙烯基的单体如甲基丙烯酸正丁酯。

5. 发明授权

US5858695A Method of quantitative determination of bilirubin and a reagent therefor 失效
标题翻译：定量测定胆红素及其试剂的方法
公开(公告)号：US5858695A
公开(公告)日：1999-01-12
申请号：US736058
申请日：1996-10-22
申请人： Akira Kadota , Kayoko Shigenobu , Akira Miike , Kazuhito Miyauchi
发明人： Akira Kadota , Kayoko Shigenobu , Akira Miike , Kazuhito Miyauchi
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26
摘要： Disclosed are a method for quantitative determination of bilirubin and a reagent for the method, which are useful in clinical examinations. The method comprises subjecting the bilirubin in a sample to coexist with ascorbate oxidase and a reaction promoter in aqueous medium to thereby oxidize the bilirubin, measuring the change in absorbance of the aqueous medium and comparing the change in absorbance with a calibration curve. The reagent comprises ascorbate oxidase and a reaction promoter.
摘要翻译：公开了用于定量测定胆红素和该方法的试剂的方法，其可用于临床检查。该方法包括使样品中的胆红素与水性介质中的抗坏血酸氧化酶和反应促进剂共存，从而氧化胆红素，测量水性介质的吸光度变化，并将吸光度的变化与校准曲线进行比较。试剂包含抗坏血酸氧化酶和反应促进剂。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式