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1. 发明申请

US20050219686A1 Microscope 审中-公开
标题翻译：显微镜
公开(公告)号：US20050219686A1
公开(公告)日：2005-10-06
申请号：US11085475
申请日：2005-03-22
申请人： Kazuhiko Osa , Yasunori Makara
发明人： Kazuhiko Osa , Yasunori Makara
IPC分类号： G02B21/00
CPC分类号： G02B21/0028
摘要： A laser scanning-type confocal microscope is provided, which can obtain a clear image at all times without being affected by pulsation or the like of a sample. The laser scanning-type confocal microscope comprises an abutting member at an end of an objective optical system to be arranged close to the sample, which is capable of moving in an optical axis direction and being positioned at a desired position in the optical axis direction with respect to the objective optical system.
摘要翻译：提供了一种激光扫描式共聚焦显微镜，可以始终获得清晰的图像，而不受样品的脉动等的影响。激光扫描型共焦显微镜包括在物镜光学系统的端部处的靠近样品布置的抵接构件，该邻接构件能够在光轴方向上移动并且位于光轴方向上的期望位置，并且尊重客观的光学系统。

2. 发明授权

US07385758B2 Total internal reflection fluorescence microscope 有权
标题翻译：全内反射荧光显微镜
公开(公告)号：US07385758B2
公开(公告)日：2008-06-10
申请号：US10816489
申请日：2004-03-31
申请人： Yasushi Aono , Tsuyoshi Mochizuki , Kazuhiko Osa
发明人： Yasushi Aono , Tsuyoshi Mochizuki , Kazuhiko Osa
IPC分类号： G02B21/06
CPC分类号： G01N21/6458 , G01N21/648 , G02B21/16
摘要： A condenser lens is disposed in a position facing an objective lens via a specimen, a reflection mirror is movably disposed in the vicinity of an outermost part of a transmitted illuminative light path on the side of a transmitted illuminative light source from the condenser lens, and a laser beam output from a laser oscillation unit is reflected by the reflection mirror and is incident upon the condenser lens.
摘要翻译：聚光透镜通过试样设置在面向物镜的位置，反射镜可移动地设置在透镜照明光路的从聚光透镜侧透射的照明光源侧的最外部附近，以及从激光振荡单元输出的激光束被反射镜反射并入射到聚光透镜。

3. 发明授权

US06829051B2 Optical apparatus 有权
标题翻译：光学仪器
公开(公告)号：US06829051B2
公开(公告)日：2004-12-07
申请号：US10189622
申请日：2002-07-08
申请人： Katsuyuki Abe , Kazuhiko Osa
发明人： Katsuyuki Abe , Kazuhiko Osa
IPC分类号： G01N2164
CPC分类号： G01N21/6458
摘要： An optical apparatus including an illuminating optical system for illuminating a sample with light from a light source, a first wavelength selecting member, a light beam splitter for directing illuminating light toward the sample, an objective lens, a detector, and a second wavelength selecting member disposed between the objective lens and the detector, in which the first wavelength selecting member has a wavelength selecting element for selectively transmitting light having wavelengths in a predetermined region out of the illuminating light, the second wavelength selecting member has a wavelength selecting element for transmitting light having a predetermined wavelength out of light reflected by the sample, and the light beam splitter has an optical element having transmittance not lower than 85% and reflectance not higher than 14% in a wavelength region not shorter than 400 nm and not longer than 700 nm. The optical apparatus permits observing fluorescent light at the same time with a high efficiency.
摘要翻译：一种光学装置，包括：用来自光源的光照射样品的照明光学系统;第一波长选择部件;将光照射到样品的光束分离器;物镜;检测器;以及第二波长选择部件配置在所述物镜与所述检测器之间，所述第一波长选择部件具有用于选择性地透过所述照明光中的预定区域的波长的光的波长选择元件，所述第二波长选择部件具有用于透射光的波长选择元件具有由样品反射的光的预定波长，并且光束分离器具有在不小于400nm且不大于700nm的波长区域中具有不低于85％的透射率和不高于14％的透射率的光学元件。光学装置允许以高效率同时观察荧光。

4. 发明授权

US06643061B2 Microscope transmitted-illumination apparatus 失效
公开(公告)号：US06643061B2
公开(公告)日：2003-11-04
申请号：US10114529
申请日：2002-04-01
申请人： Kazuhiko Osa , Minoru Sukekawa , Kenji Kawasaki
发明人： Kazuhiko Osa , Minoru Sukekawa , Kenji Kawasaki
IPC分类号： G02B2106
CPC分类号： G02B21/06 , G02B21/086
摘要： In the present invention, a transmission-illumination apparatus for use in a microscope has a transmission-illumination optical system comprising a condenser lens for radiating light emitted from a light source onto a sample, and an observation optical system including an objective lens for observing the sample. At least two shield members for controlling a shape of an aperture created in a pupil of the objective lens are disposed at or near positions conjugate with a pupil position of the objective lens. Thereby, a contrast can be successively varied without disposing a purpose-specific optical element, etc. in the observation optical system, and illumination with an optimal contrast can be provided for various samples.

5. 外观设计

USD445813S1 Body of microscope 失效
标题翻译：显微镜身体
公开(公告)号：USD445813S1
公开(公告)日：2001-07-31
申请号：US29129507
申请日：2000-09-15
申请人： Takeshi Asaka , Kouji Suzuki , Kazuhiko Osa
设计人： Takeshi Asaka , Kouji Suzuki , Kazuhiko Osa

6. 发明授权

US06396628B1 Microscope transmitted-illumination apparatus 有权
公开(公告)号：US06396628B1
公开(公告)日：2002-05-28
申请号：US09514863
申请日：2000-02-28
申请人： Kazuhiko Osa , Minoru Sukekawa , Kenji Kawasaki
发明人： Kazuhiko Osa , Minoru Sukekawa , Kenji Kawasaki
IPC分类号： G02B2106
CPC分类号： G02B21/06 , G02B21/086
摘要： In the present invention, a transmission-illumination apparatus for use in a microscope has a transmission-illumination optical system comprising a condenser lens for radiating light emitted from a light source onto a sample, and an observation optical system including an objective lens for observing the sample. At least two shield members for controlling a shape of an aperture created in a pupil of the objective lens are disposed at or near positions conjugate with a pupil position of the objective lens. Thereby, a contrast can be successively varied without disposing a purpose-specific optical element, etc. in the observation optical system, and illumination with an optimal contrast can be provided for various samples.

7. 发明申请

US20060050276A1 Dark box apparatus for fluoroscopy, fluoroscopy system, and fluoroscopy method 有权
标题翻译：用于荧光透视，荧光透视系统和荧光透视法的暗盒装置
公开(公告)号：US20060050276A1
公开(公告)日：2006-03-09
申请号：US11213906
申请日：2005-08-30
申请人： Kei Tsuyuki , Kazuhiko Osa , Nobuyuki Nagasawa
发明人： Kei Tsuyuki , Kazuhiko Osa , Nobuyuki Nagasawa
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G01N21/6456
摘要： Noise in a fluorescence image acquired during fluoroscopy is eliminated to present a clear fluorescence image, and the relative positional relationship between the fluoroscopy unit and the specimen can be recognized even while fluoroscopy is in progress. A dark box apparatus for fluoroscopy includes: a dark-box main body enclosing a specimen and a fluoroscopy unit for illuminating the specimen with excitation light with a first spectral band and for detecting fluorescence with a second spectral band generated by the specimen; an illumination light source disposed in the dark-box main body to emit light with a third spectral band different from the first spectral band and the second spectral band; and an observation window disposed in the dark-box main body, the observation window being capable of transmitting light with a fourth spectral band which includes at least part of the third spectral band and does not include the first spectral band and the second spectral band.
摘要翻译：在荧光透视期间获取的荧光图像中的噪声被消除以呈现清晰的荧光图像，并且即使在进行荧光透视时也能够识别透视单元和样本之间的相对位置关系。用于荧光透视的暗盒装置包括：包围样本的暗盒主体和用于用具有第一光谱带的激发光照射样本并用于用由样本产生的第二光谱带检测荧光的荧光透视单元; 照明光源，其设置在所述暗盒主体内，以与所述第一光谱带和所述第二光谱带不同的第三光谱带发光; 以及设置在暗箱主体中的观察窗，所述观察窗能够透射具有包括所述第三光谱带的至少一部分的第四光谱带的光，并且不包括所述第一光谱带和所述第二光谱带。

8. 发明授权

US5325231A Microscope illuminating apparatus 失效
标题翻译：显微镜照明设备
公开(公告)号：US5325231A
公开(公告)日：1994-06-28
申请号：US854333
申请日：1992-03-19
申请人： Keisuke Tamura , Kazuhiko Osa , Takashi Nagano
发明人： Keisuke Tamura , Kazuhiko Osa , Takashi Nagano
IPC分类号： G02B21/08 , G02B21/10 , G02B21/06 , G02B7/24
CPC分类号： G02B21/088 , G02B21/10
摘要： A microscope illuminating apparatus includes a light-shielding tube surrounding an objective lens, detachably mounted on a revolver of a system microscope in order to guide illumination light to irradiate an observation field, thus separating an illumination optical path and an observation optical path. An annular illuminating section serving as a light source of the illumination light is provided at a position to oppose an end portion aperture of the illumination optical path in the revolver.
摘要翻译：显微镜照明装置包括围绕物镜的遮光管，可拆卸地安装在系统显微镜的左轮上以引导照明光照射观察场，从而分离照明光路和观察光路。作为照明光的光源的环状照明部设置在与左轮中的照明光路的端部孔相对的位置。

9. 发明申请

US20070177142A1 Dark box apparatus for fluoroscopy, fluoroscopy system, and fluoroscopy method 有权
标题翻译：用于荧光透视，荧光透视系统和荧光透视法的暗盒装置
公开(公告)号：US20070177142A1
公开(公告)日：2007-08-02
申请号：US11726885
申请日：2007-03-23
申请人： Kei Tsuyuki , Kazuhiko Osa , Nobuyuki Nagasawa
发明人： Kei Tsuyuki , Kazuhiko Osa , Nobuyuki Nagasawa
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G01N21/6456
摘要： Noise in a fluorescence image acquired during fluoroscopy is eliminated to present a clear fluorescence image, and the relative positional relationship between the fluoroscopy unit and the specimen can be recognized even while fluoroscopy is in progress. A dark box apparatus for fluoroscopy includes: a dark-box main body enclosing a specimen and a fluoroscopy unit for illuminating the specimen with excitation light with a first spectral band and for detecting fluorescence with a second spectral band generated by the specimen; an illumination light source disposed in the dark-box main body to emit light with a third spectral band different from the first spectral band and the second spectral band; and an observation window disposed in the dark-box main body, the observation window being capable of transmitting light with a fourth spectral band which includes at least part of the third spectral band and does not include the first spectral band and the second spectral band.
摘要翻译：在荧光透视期间获取的荧光图像中的噪声被消除以呈现清晰的荧光图像，并且即使在进行荧光透视时也能够识别透视单元和样本之间的相对位置关系。用于荧光透视的暗盒装置包括：包围样本的暗盒主体和用于用具有第一光谱带的激发光照射样本并用于用由样本产生的第二光谱带检测荧光的荧光透视单元; 照明光源，其设置在所述暗盒主体内，以与所述第一光谱带和所述第二光谱带不同的第三光谱带发光; 以及设置在暗箱主体中的观察窗，所述观察窗能够透射具有包括所述第三光谱带的至少一部分的第四光谱带的光，并且不包括所述第一光谱带和所述第二光谱带。

10. 发明授权

US07215426B2 Dark box apparatus for fluoroscopy, fluoroscopy system, and fluoroscopy method 有权
公开(公告)号：US07215426B2
公开(公告)日：2007-05-08
申请号：US11213906
申请日：2005-08-30
申请人： Kei Tsuyuki , Kazuhiko Osa , Nobuyuki Nagasawa
发明人： Kei Tsuyuki , Kazuhiko Osa , Nobuyuki Nagasawa
IPC分类号： G01N21/64
CPC分类号： G01N21/6458 , G01N21/6456
摘要： Noise in a fluorescence image acquired during fluoroscopy is eliminated to present a clear fluorescence image, and the relative positional relationship between the fluoroscopy unit and the specimen can be recognized even while fluoroscopy is in progress. A dark box apparatus for fluoroscopy includes: a dark-box main body enclosing a specimen and a fluoroscopy unit for illuminating the specimen with excitation light with a first spectral band and for detecting fluorescence with a second spectral band generated by the specimen; an illumination light source disposed in the dark-box main body to emit light with a third spectral band different from the first spectral band and the second spectral band; and an observation window disposed in the dark-box main body, the observation window being capable of transmitting light with a fourth spectral band which includes at least part of the third spectral band and does not include the first spectral band and the second spectral band.

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