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1. 发明申请

WO2008013405A1 NOVEL ENGINEERED MICROORGANISM PRODUCING HOMO-SUCCINIC ACID AND METHOD FOR PREPARING SUCCINIC ACID USING THE SAME 审中-公开
标题翻译：生产同型酸的新型工程化微生物及其制备方法
公开(公告)号：WO2008013405A1
公开(公告)日：2008-01-31
申请号：PCT/KR2007/003574
申请日：2007-07-25
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LIM, Sung Won , SONG, Hyohak
发明人： LEE, Sang Yup , LIM, Sung Won , SONG, Hyohak
IPC分类号： C12N1/21
CPC分类号： C12P7/46 , C12N1/20 , C12N9/0006 , C12N9/1029 , C12N9/1217 , C12N15/902 , C12Y203/01054 , Y02P20/52
摘要： The present invention relates to a rumen bacterial mutant producing homo- succinic acid and a method for producing homo-succinic acid using the same, and more particularly to a rumen bacterial mutant producing succinic acid at a high concentration while producing little or no other organic acids in anaerobic conditions, which is obtained by disrupting a gene encoding lactate dehydrogenase (idhA), a gene encoding phosphotransacetylase (pta), and a gene encoding acetate kinase (ackA), without disrupting a gene encoding pyruvate formate lyase (pβ), as well as a method for producing succinic acid using the same. The inventive rumen bacterial mutant has the property of having a high growth rate and succinic acid productivity while producing little or no organic acids, as compared to the prior strains producing succinic acid. Thus, the inventive rumen bacterial mutant is useful to produce succinic acid for industrial use.
摘要翻译：本发明涉及产生同型琥珀酸的瘤胃细菌突变体和使用其的均琥珀酸的制备方法，更具体地说，涉及产生很少或不含其它有机酸的高浓度琥珀酸的瘤胃细菌突变体在厌氧条件下，其通过破坏编码乳酸脱氢酶（idhA）的基因，编码磷酸转乙酰酶（pta）的基因和编码乙酸激酶（ackA）的基因而不破坏编码丙酮酸甲酯裂解酶（pβ）的基因而得到作为使用其生产琥珀酸的方法。与生产琥珀酸的现有菌株相比，本发明的瘤胃细菌突变体具有高生长速率和琥珀酸生产力，同时产生很少或没有有机酸的性质。因此，本发明的瘤胃细菌突变体可用于生产用于工业用途的琥珀酸。

2. 发明申请

WO2009078687A3 RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE 审中-公开
公开(公告)号：WO2009078687A3
公开(公告)日：2009-06-25
申请号：PCT/KR2008/007533
申请日：2008-12-18
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
发明人： LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
IPC分类号： C12N9/10 , C12N9/26 , C12N15/09 , C12N15/00
摘要： The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.

3. 发明申请

WO2009008574A1 HOMO-SUCCINIC ACID PRODUCING MICROORGANISM VARIANT AND PROCESS FOR PREPARING SUCCINIC ACID USING THE SAME 审中-公开
标题翻译：制备微生物的肝细胞生长因子及其制备方法
公开(公告)号：WO2009008574A1
公开(公告)日：2009-01-15
申请号：PCT/KR2008/000241
申请日：2008-01-15
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , KIM, Ji Mahn , LEE, Jeong Wook , SONG, Hyohak
发明人： LEE, Sang Yup , KIM, Ji Mahn , LEE, Jeong Wook , SONG, Hyohak
IPC分类号： C12N15/04 , C12N1/15
CPC分类号： C12P7/46 , C12N9/0006 , C12N9/1217
摘要： The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydrogenase-encoding gene ( idhA ) and an acetate kinase-encoding gene ( ackA ), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.
摘要翻译：本发明涉及以高产率产生高聚琥珀酸的微生物变异体，以及使用该方法生产同型琥珀酸的方法，更具体地说，涉及通过破坏编码乳酸脱氢酶基因（idhA）和乙酸盐激酶编码基因（ackA）以及高浓度均聚琥珀酸的方法，包括在厌氧条件下使用葡萄糖作为碳源培养这些变体。

4. 发明申请

WO2009082050A1 METHOD FOR PURIFYING SUCCINIC ACID BY CRYSTALLIZATION OF CULTURE BROTH 审中-公开
标题翻译：通过文化结晶的纯化方法
公开(公告)号：WO2009082050A1
公开(公告)日：2009-07-02
申请号：PCT/KR2008/000238
申请日：2008-01-15
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , HONG, Won Hi , LEE, Sang Yup , HONG, Yeon Ki , WON, Hyo Jin , HUH, Yun Suk , SONG, Hyohak , LEE, Eun Zoo
发明人： HONG, Won Hi , LEE, Sang Yup , HONG, Yeon Ki , WON, Hyo Jin , HUH, Yun Suk , SONG, Hyohak , LEE, Eun Zoo
IPC分类号： C12P7/40
CPC分类号： C07C51/44 , C07C51/43 , C07C51/47 , C07C55/10
摘要： The present invention relates to a method for separating and purifying succinic acid with high purity and high yield by crystallization of culture broth and, more particularly, a method for recovering succinic acid with high purity and high yield, which comprises concentrating culture broth from which succinic acid-producing microorganism is removed and then adding an acid solution at low temperature, thus directly crystallizing without other pretreatment processes. According to the present invention, succinic acid is separated and purified using culture broth, from which succinic acid producing microorganism is removed, without other pretreatment processes, and thus a cost-saving effect due to process simplification, and an effect of environmental pollution prevention due to the prevention of sludge generation during succinic acid recovery process, can be achieved. In addition, succinic acid was recovered with high purity and high yield, thus making it possible to achieve a technical effect unprecedented in the prior art in terms of cost efficiency.
摘要翻译：本发明涉及通过培养液结晶分离和纯化琥珀酸的高纯度和高产率的方法，更具体地说，涉及一种以高纯度和高收率回收琥珀酸的方法，该方法包括将培养液从琥珀酸除去酸产生微生物，然后在低温下加入酸溶液，从而直接结晶而无需其它预处理方法。根据本发明，使用除去琥珀酸微生物的培养液分离和纯化琥珀酸，而无需其它预处理过程，因此由于过程简化而导致的成本节约效果以及由此引起的环境污染防治的效果为了防止在琥珀酸回收过程中产生污泥，可以实现。此外，以高纯度和高收率回收琥珀酸，从而在成本效率方面可以实现现有技术中前所未有的技术效果。

5. 发明申请

WO2009048202A1 METHOD FOR PREPARING SUCCINIC ACID USING GLYCEROL AS CARBON SOURCE 审中-公开
标题翻译：使用甘油作为碳源制备磺酸的方法
公开(公告)号：WO2009048202A1
公开(公告)日：2009-04-16
申请号：PCT/KR2008/000237
申请日：2008-01-15
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , CHOI, Sol , KIM, Ji Mahn
发明人： LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , CHOI, Sol , KIM, Ji Mahn
IPC分类号： C12P7/40
CPC分类号： C12P7/46
摘要： The present invention relates to a method for preparing succinic acid using glycerol as a carbon source, and more particularly, a method for preparing succinic acid with high purity and high yield, which comprises inoculating Mannheimia sp., which is a facultative anaerobic microorganism, into a culture medium containing glycerol as a carbon source and anaerobically culturing, then recovering succinic acid. According to the present invention, a significant amount of succinic acid can be produced compared with the methods using carbon sources such as glucose or sucrose, and formation of total by-products is inhibited or prevented upon microbial fermentation, thus making it possible to reduce costs required for separating and purifying succinic acid from final culture broth.
摘要翻译：本发明涉及一种使用甘油作为碳源制备琥珀酸的方法，更具体地说，涉及一种制备高纯度和高产率的琥珀酸的方法，其中包括将作为兼性厌氧微生物的Mannheimia sp。将含有甘油作为碳源的培养基进行厌氧培养，然后回收琥珀酸。根据本发明，与使用碳源如葡萄糖或蔗糖的方法相比，可以生产显着量的琥珀酸，并且在微生物发酵时抑制或防止总副产物的形成，从而可以降低成本从最终培养液中分离和纯化琥珀酸所需的。

6. 发明申请

WO2009014289A1 METHOD FOR PREPARING SUCCINIC ACID USING SUCROSE AS A CARBON SOURCE 审中-公开
标题翻译：使用蔗糖作为碳源制备磺酸的方法
公开(公告)号：WO2009014289A1
公开(公告)日：2009-01-29
申请号：PCT/KR2008/000242
申请日：2008-01-15
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , SONG, Hyohak , LEE, Jeong Wook , CHOI, Sol , KIM, Ji Mahn
发明人： LEE, Sang Yup , SONG, Hyohak , LEE, Jeong Wook , CHOI, Sol , KIM, Ji Mahn
IPC分类号： C12P7/40
CPC分类号： C12P7/46
摘要： The present invention relates to a method for preparing succinic acid, which comprises culturing a succinic acid-producing microorganism in a medium containing sucrose as a carbon source, and more particularly, a method for preparing succinic acid, which comprises culturing a succinic acid-producing microorganism under batch or fed-batch culture conditions in a medium containing sucrose as a carbon source at a high concentration. According to the present invention, when inexpensive sucrose, whose price corresponds to 28.9% of glucose price, is used as a carbon source, microorganisms have improved resistance against organic acids including succinic acid compared to the case using other carbon sources such as glucose to significantly increase succinic acid productivity as well as final succinic acid concentration, thus making it possible to reduce costs required for separating and purifying succinic acid.
摘要翻译：本发明涉及一种制备琥珀酸的方法，其包括在含有蔗糖的碳源的培养基中培养产琥珀酸微生物，更具体地说，涉及制备琥珀酸的方法，其包括培养产生琥珀酸的琥珀酸在分批或补料分批培养条件下在含有高浓度蔗糖作为碳源的培养基中的微生物。根据本发明，当价格对应于葡萄糖价格的28.9％的廉价蔗糖用作碳源时，与使用其它碳源如葡萄糖的情况相比，微生物对包括琥珀酸在内的有机酸的耐受性提高，提高琥珀酸生产率以及最终的琥珀酸浓度，从而可以降低分离和纯化琥珀酸所需的成本。

7. 发明申请

WO2008088104A1 MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 审中-公开
标题翻译：具有改进的分支氨基酸生产能力的突变微生物及使用其制备分支氨基酸的方法
公开(公告)号：WO2008088104A1
公开(公告)日：2008-07-24
申请号：PCT/KR2007/001263
申请日：2007-03-14
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
发明人： LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
IPC分类号： C12N1/16
CPC分类号： C12P13/08 , C12N9/1014 , C12Y201/02011
摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site- specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.
摘要翻译：本发明涉及具有提高的支链氨基酸生产力的突变微生物，以及使用突变微生物生产支链氨基酸的方法。更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

8. 发明申请

WO2006126750A1 METHOD FOR PREPARING A TARGET PROTEIN USING THE sHSPs 审中-公开
标题翻译：使用sHSP制备目标蛋白的方法
公开(公告)号：WO2006126750A1
公开(公告)日：2006-11-30
申请号：PCT/KR2005/001500
申请日：2005-05-23
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , HAN, Mee Jung
发明人： LEE, Sang Yup , HAN, Mee Jung
IPC分类号： C07K1/14
CPC分类号： C07K1/14
摘要： The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs are added in cultivation, separation and purification processes for preparing a target protein, the target protein can be obtained at high yields by preventing the loss of protein by proteases. Also, when sHSPs are added in a reaction process using a whole cell enzyme or a partially purified enzyme, the yield of bioconversion using enzyme can be increased by preventing the loss of enzyme by proteases.
摘要翻译：本发明涉及用于分离和纯化靶蛋白的方法，制备靶蛋白的方法，以及由全细胞酶或部分纯化的酶进行生物转化的方法。根据本发明，为了制备目标蛋白质的培养，分离纯化方法添加sHSP，可以通过防止蛋白酶的蛋白质损失而以高收率获得目标蛋白质。此外，当使用全细胞酶或部分纯化的酶在反应过程中加入sHSP时，可以通过防止蛋白酶损失酶来增加使用酶的生物转化产率。

9. 发明申请

WO2004061453A1 A PROTEIN CHIP FOR ANALYZING INTERACTION BETWEEN PROTEIN AND SUBSTRATE PEPTIDE THEREOF 审中-公开
标题翻译：蛋白质芯片，用于分析蛋白质与其底物之间的相互作用
公开(公告)号：WO2004061453A1
公开(公告)日：2004-07-22
申请号：PCT/KR2003/002183
申请日：2003-10-18
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Seok Jae
发明人： LEE, Sang Yup , LEE, Seok Jae
IPC分类号： G01N33/543
CPC分类号： C40B30/04 , C07K1/1077 , C07K14/5759 , C12N9/0006 , G01N33/54353 , G01N33/6803
摘要： The present invention relates to a protein chip of a S-L-SP form wherein a substrate peptide (SP) is immobilized on a solid substrate (S) by the mediation of a linker protein (L), as well as a method for analyzing the interaction between a reactive protein and its substrate peptide using such a protein chip. This analysis method for the interaction between a reactive protein and its substrate peptide using the protein chip comprises the steps of: adding a reactive protein to the protein chip, the reactive protein showing a specific interaction with the substrate protein immobilized on the protein chip; and detecting the interaction between the reactive protein and the substrate peptide. The present invention allows an increase in the reactivity between a peptide with low molecular weight and an enzyme with high molecular weight and between the peptide and a reactive antibody on the protein chip, so that the interaction between the peptide and the protein can be analyzed rapidly and massively.
摘要翻译：本发明涉及SL-SP形式的蛋白质芯片，其中通过连接蛋白（L）的介导将底物肽（SP）固定在固体底物（S）上，以及分析相互作用的方法在使用这种蛋白质芯片的反应蛋白和其底物肽之间。使用蛋白质芯片的反应蛋白与其底物肽之间的相互作用的分析方法包括以下步骤：向蛋白质芯片添加反应蛋白，反应蛋白与固定在蛋白质芯片上的底物蛋白显示特异性相互作用; 并检测反应蛋白和底物肽之间的相互作用。本发明允许增加低分子量肽和高分子量酶之间以及蛋白质芯片上的肽与反应性抗体之间的反应性，从而可以快速分析肽与蛋白质之间的相互作用并大量。

10. 发明申请

WO2008111708A1 L-THREONINE OVERPRODUCING MICROORGANISM AND METHOD FOR PREPARING L-THREONINE USING THE SAME 审中-公开
标题翻译： L-THREONINE OVERPRODUCTION MICROORGANISM AND METHOD FOR PRIPARING L-THREONINE WITH THE SAME
公开(公告)号：WO2008111708A1
公开(公告)日：2008-09-18
申请号：PCT/KR2007/003200
申请日：2007-07-02
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Kwang Ho , PARK, Jin Hwan , KIM, Tae Yong
发明人： LEE, Sang Yup , LEE, Kwang Ho , PARK, Jin Hwan , KIM, Tae Yong
IPC分类号： C12N15/09 , C12N1/21 , C12P13/08
CPC分类号： C12P13/08
摘要： The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.
摘要翻译：本发明涉及使用位点特异性突变而不是任意突变（例如用突变诱导剂处理）制备高产量的L-苏氨酸的高浓度的突变型微生物，其制备方法和使用产生L-苏氨酸的突变体微生物制备L-苏氨酸。通过使用根据本发明的突变微生物，可以高产率制备L-苏氨酸，由于可以鉴定L-苏氨酸生产微生物的遗传信息，所以可以进行附加应变发展，并且可以容易地理解其生理现象。

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