专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2008111708A1 L-THREONINE OVERPRODUCING MICROORGANISM AND METHOD FOR PREPARING L-THREONINE USING THE SAME 审中-公开
标题翻译： L-THREONINE OVERPRODUCTION MICROORGANISM AND METHOD FOR PRIPARING L-THREONINE WITH THE SAME
公开(公告)号：WO2008111708A1
公开(公告)日：2008-09-18
申请号：PCT/KR2007/003200
申请日：2007-07-02
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Kwang Ho , PARK, Jin Hwan , KIM, Tae Yong
发明人： LEE, Sang Yup , LEE, Kwang Ho , PARK, Jin Hwan , KIM, Tae Yong
IPC分类号： C12N15/09 , C12N1/21 , C12P13/08
CPC分类号： C12P13/08
摘要： The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.
摘要翻译：本发明涉及使用位点特异性突变而不是任意突变（例如用突变诱导剂处理）制备高产量的L-苏氨酸的高浓度的突变型微生物，其制备方法和使用产生L-苏氨酸的突变体微生物制备L-苏氨酸。通过使用根据本发明的突变微生物，可以高产率制备L-苏氨酸，由于可以鉴定L-苏氨酸生产微生物的遗传信息，所以可以进行附加应变发展，并且可以容易地理解其生理现象。

2. 发明申请

WO2008088104A1 MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 审中-公开
标题翻译：具有改进的分支氨基酸生产能力的突变微生物及使用其制备分支氨基酸的方法
公开(公告)号：WO2008088104A1
公开(公告)日：2008-07-24
申请号：PCT/KR2007/001263
申请日：2007-03-14
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
发明人： LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
IPC分类号： C12N1/16
CPC分类号： C12P13/08 , C12N9/1014 , C12Y201/02011
摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site- specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.
摘要翻译：本发明涉及具有提高的支链氨基酸生产力的突变微生物，以及使用突变微生物生产支链氨基酸的方法。更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

3. 发明申请

WO2009078687A3 RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE 审中-公开
公开(公告)号：WO2009078687A3
公开(公告)日：2009-06-25
申请号：PCT/KR2008/007533
申请日：2008-12-18
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
发明人： LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
IPC分类号： C12N9/10 , C12N9/26 , C12N15/09 , C12N15/00
摘要： The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.

4. 发明申请

WO2008072921A1 ENHANCED BUTANOL PRODUCING MICROORGANISMS AND METHOD FOR PREPARING BUTANOL USING THE SAME 审中-公开
标题翻译：生产微生物的增强的丁醇和使用它们制备丁醇的方法
公开(公告)号：WO2008072921A1
公开(公告)日：2008-06-19
申请号：PCT/KR2007/006525
申请日：2007-12-14
申请人： BIOFUELCHEM CO., LTD. , LEE, Sang Yup , PARK, Jin Hwan , PAPOUTSAKIS, Eleftherios, Terry
发明人： LEE, Sang Yup , PARK, Jin Hwan , PAPOUTSAKIS, Eleftherios, Terry
IPC分类号： C12N15/00
CPC分类号： C12P7/16 , Y02E50/10
摘要： The present invention relates to a recombinant mutant microorganism having enhanced butanol producing capacity and a method for producing butanol using the same. In the microorganism, genes coding for enzymes responsible for the biosynthesis of lactate, ethanol and/or acetate are deleted or attenuated and genes coding for enzymes involved in butanol biosynthesis are introduced and amplified.
摘要翻译：本发明涉及具有提高的丁醇生产能力的重组突变体微生物和使用其的丁醇生产方法。在微生物中，编码负责乳酸盐，乙醇和/或乙酸盐的生物合成的酶的基因被缺失或减弱，并且引入并扩增了参与丁醇生物合成的酶编码基因。

5. 发明申请

WO2008072920A1 METHOD FOR PREPARING BUTANOL THROUGH BUTYRYL-COA AS AN INTERMEDIATE USING BACTERIA 审中-公开
标题翻译：通过丙二醇作为中间体使用细菌制备丁醇的方法
公开(公告)号：WO2008072920A1
公开(公告)日：2008-06-19
申请号：PCT/KR2007/006524
申请日：2007-12-14
申请人： BIOFUELCHEM CO., LTD. , PAPOUTSAKIS, Eleftherios, Terry , LEE, Sang Yup , PARK, Jin Hwan
发明人： PAPOUTSAKIS, Eleftherios, Terry , LEE, Sang Yup , PARK, Jin Hwan
IPC分类号： C12P7/16 , C12N1/21
CPC分类号： C12P7/16 , Y02E50/10
摘要： The present invention relates to a method for producing butanol using a bacterium capable of biosynthesizing butanol from butyryl-CoA as an intermediate. More particularly, a method for producing butanol, the method comprising generating bytyryl-CoA in a bacterium which contains a gene coding for AdhE (an enzyme responsible for the conversion of butyryl-CoA to butanol) using various methods, and converting the butyryl-CoA into butanol.
摘要翻译：本发明涉及使用能够以丁酰辅酶A作为中间体生物合成丁醇的细菌生产丁醇的方法。更具体地，涉及一种生产丁醇的方法，该方法包括使用各种方法在含有编码AdhE的基因（负责将丁酰辅酶A转化为丁醇的酶）的细菌中生成二丁基-CoA，并将丁酰辅酶A 变成丁醇。

6. 发明公开

EP2481793A2 RECOMBINANT MICROORGANISM WITH INCREASED BUTANOL PRODUCTION ABILITY, AND PREPARATION METHOD OF BUTANOL USING SAME 有权转让
标题翻译：随着对丁醇INCREASEDBUTANOLHERSTELLUNGSFÄHIGKEIT和方法重组微生物SO
公开(公告)号：EP2481793A2
公开(公告)日：2012-08-01
申请号：EP10819056.2
申请日：2010-09-21
申请人： Korea Advanced Institute of Science and Technology , Biofuelchem Co. , GS Caltex Corporation
发明人： LEE, Sang Yup , JANG, Yu-Sin , LEE, Jin Young , LEE, Joung Min , PARK, Jin Hwan
IPC分类号： C12N1/21 , C12P7/16 , C12N15/52
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.

7. 发明授权

EP2481793B1 RECOMBINANT MICROORGANISM WITH INCREASED BUTANOL PRODUCTION ABILITY, AND PREPARATION METHOD OF BUTANOL USING SAME 有权转让
标题翻译：重组微生物提高丁醇生产能力，以及使用相同的丁醇制备方法
公开(公告)号：EP2481793B1
公开(公告)日：2017-08-30
申请号：EP10819056.2
申请日：2010-09-21
申请人： Korea Advanced Institute of Science and Technology , Biofuelchem Co. , GS Caltex Corporation
发明人： LEE, Sang Yup , JANG, Yu-Sin , LEE, Jin Young , LEE, Joung Min , PARK, Jin Hwan
IPC分类号： C12N1/21 , C12P7/16 , C12N15/52
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/1029 , C12Y101/01001 , C12Y203/01008 , C12Y203/01019 , Y02E50/10
摘要： The present invention relates to recombinant microorganisms having an increased ability to produce butanol, and a method of producing butanol using the same. More specifically, the invention relates to recombinant microorganisms whose ability to produce butanol was increased by manipulation of their metabolic networks, and a method of producing butanol using the same. The recombinant microorganisms having an increased ability to produce butanol comprise a deletion of a gene, which encodes an enzyme that converts acetyl CoA to acetate, in host microorganisms having genes that encode enzymes involved in acetyl CoA and butyryl CoA biosynthetic pathway. The recombinant microorganisms obtained by manipulating the metabolic flux of microorganisms are able to selectively produce butanol with high efficiency, and thus are useful as microorganisms for producing industrial solvents and transportation fuels.
摘要翻译：本发明涉及具有提高的丁醇生产能力的重组微生物以及使用该微生物生产丁醇的方法。更具体地说，本发明涉及通过操作其代谢网络来增加丁醇生产能力的重组微生物，以及使用该微生物生产丁醇的方法。具有增加的丁醇生产能力的重组微生物包括在编码参与乙酰辅酶A和丁酰辅酶A生物合成途径的酶的基因的宿主微生物中缺失编码将乙酰辅酶A转化为乙酸的酶的基因。通过操作微生物的代谢流而获得的重组微生物能够高效选择性地生产丁醇，因此可用作用于生产工业溶剂和运输燃料的微生物。

8. 发明公开

EP2038403A1 MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 审中-公开
标题翻译：支链氨基酸提高生产能力的突变微生物及用其制备支链氨基酸的方法
公开(公告)号：EP2038403A1
公开(公告)日：2009-03-25
申请号：EP07715659.4
申请日：2007-03-14
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
IPC分类号： C12N1/16
CPC分类号： C12P13/08 , C12N9/1014 , C12Y201/02011
摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site- specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.
摘要翻译：本发明涉及具有提高的支链氨基酸生产率的突变微生物以及使用该突变微生物生产支链氨基酸的方法。更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码参与L-亮氨酸的酶的基因和编码L-异亮氨酸生物合成酶的基因而产生的L-缬氨酸的生产率提高的突变微生物参与D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，从而增加其表达，以及使用该突变体微生物生产L-缬氨酸的方法。通过位点特异性诱变和代谢途径工程产生的本发明的突变微生物可高效生产支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

9. 发明授权

EP2121918B1 L-THREONINE OVERPRODUCING MICROORGANISM AND METHOD FOR PREPARING L-THREONINE USING THE SAME 有权
标题翻译： L-苏氨酸关于生产微生物群落和SO生产L-苏氨酸方法
公开(公告)号：EP2121918B1
公开(公告)日：2011-09-28
申请号：EP07768570.9
申请日：2007-07-02
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , LEE, Kwang Ho , PARK, Jin Hwan , KIM, Tae Yong, Korea Advanced Institute of Science & Technology
IPC分类号： C12N15/09 , C12N1/21 , C12P13/08
CPC分类号： C12P13/08
摘要： The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.

10. 发明公开

EP2227541A2 RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE 有权
标题翻译：以能力重组微生物利用蔗糖作为碳源
公开(公告)号：EP2227541A2
公开(公告)日：2010-09-15
申请号：EP08862527.2
申请日：2008-12-18
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
IPC分类号： C12N9/10 , C12N9/26 , C12N15/09 , C12N15/00
CPC分类号： C12N15/70 , C12N1/20 , C12N9/1205 , C12N9/16 , C12N9/2408 , C12P1/04 , C12P7/065 , C12P7/40 , C12P7/46 , C12P7/54 , C12P7/56 , C12P13/08 , C12Y207/01004 , C12Y207/01069 , C12Y302/01026 , Y02E50/17
摘要： The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式