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1. 发明申请

WO2009078687A3 RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE 审中-公开
公开(公告)号：WO2009078687A3
公开(公告)日：2009-06-25
申请号：PCT/KR2008/007533
申请日：2008-12-18
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
发明人： LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
IPC分类号： C12N9/10 , C12N9/26 , C12N15/09 , C12N15/00
摘要： The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.

2. 发明申请

WO2009048202A1 METHOD FOR PREPARING SUCCINIC ACID USING GLYCEROL AS CARBON SOURCE 审中-公开
标题翻译：使用甘油作为碳源制备磺酸的方法
公开(公告)号：WO2009048202A1
公开(公告)日：2009-04-16
申请号：PCT/KR2008/000237
申请日：2008-01-15
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , CHOI, Sol , KIM, Ji Mahn
发明人： LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , CHOI, Sol , KIM, Ji Mahn
IPC分类号： C12P7/40
CPC分类号： C12P7/46
摘要： The present invention relates to a method for preparing succinic acid using glycerol as a carbon source, and more particularly, a method for preparing succinic acid with high purity and high yield, which comprises inoculating Mannheimia sp., which is a facultative anaerobic microorganism, into a culture medium containing glycerol as a carbon source and anaerobically culturing, then recovering succinic acid. According to the present invention, a significant amount of succinic acid can be produced compared with the methods using carbon sources such as glucose or sucrose, and formation of total by-products is inhibited or prevented upon microbial fermentation, thus making it possible to reduce costs required for separating and purifying succinic acid from final culture broth.
摘要翻译：本发明涉及一种使用甘油作为碳源制备琥珀酸的方法，更具体地说，涉及一种制备高纯度和高产率的琥珀酸的方法，其中包括将作为兼性厌氧微生物的Mannheimia sp。将含有甘油作为碳源的培养基进行厌氧培养，然后回收琥珀酸。根据本发明，与使用碳源如葡萄糖或蔗糖的方法相比，可以生产显着量的琥珀酸，并且在微生物发酵时抑制或防止总副产物的形成，从而可以降低成本从最终培养液中分离和纯化琥珀酸所需的。

3. 发明申请

WO2009014289A1 METHOD FOR PREPARING SUCCINIC ACID USING SUCROSE AS A CARBON SOURCE 审中-公开
标题翻译：使用蔗糖作为碳源制备磺酸的方法
公开(公告)号：WO2009014289A1
公开(公告)日：2009-01-29
申请号：PCT/KR2008/000242
申请日：2008-01-15
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , SONG, Hyohak , LEE, Jeong Wook , CHOI, Sol , KIM, Ji Mahn
发明人： LEE, Sang Yup , SONG, Hyohak , LEE, Jeong Wook , CHOI, Sol , KIM, Ji Mahn
IPC分类号： C12P7/40
CPC分类号： C12P7/46
摘要： The present invention relates to a method for preparing succinic acid, which comprises culturing a succinic acid-producing microorganism in a medium containing sucrose as a carbon source, and more particularly, a method for preparing succinic acid, which comprises culturing a succinic acid-producing microorganism under batch or fed-batch culture conditions in a medium containing sucrose as a carbon source at a high concentration. According to the present invention, when inexpensive sucrose, whose price corresponds to 28.9% of glucose price, is used as a carbon source, microorganisms have improved resistance against organic acids including succinic acid compared to the case using other carbon sources such as glucose to significantly increase succinic acid productivity as well as final succinic acid concentration, thus making it possible to reduce costs required for separating and purifying succinic acid.
摘要翻译：本发明涉及一种制备琥珀酸的方法，其包括在含有蔗糖的碳源的培养基中培养产琥珀酸微生物，更具体地说，涉及制备琥珀酸的方法，其包括培养产生琥珀酸的琥珀酸在分批或补料分批培养条件下在含有高浓度蔗糖作为碳源的培养基中的微生物。根据本发明，当价格对应于葡萄糖价格的28.9％的廉价蔗糖用作碳源时，与使用其它碳源如葡萄糖的情况相比，微生物对包括琥珀酸在内的有机酸的耐受性提高，提高琥珀酸生产率以及最终的琥珀酸浓度，从而可以降低分离和纯化琥珀酸所需的成本。

4. 发明申请

WO2009008574A1 HOMO-SUCCINIC ACID PRODUCING MICROORGANISM VARIANT AND PROCESS FOR PREPARING SUCCINIC ACID USING THE SAME 审中-公开
标题翻译：制备微生物的肝细胞生长因子及其制备方法
公开(公告)号：WO2009008574A1
公开(公告)日：2009-01-15
申请号：PCT/KR2008/000241
申请日：2008-01-15
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , KIM, Ji Mahn , LEE, Jeong Wook , SONG, Hyohak
发明人： LEE, Sang Yup , KIM, Ji Mahn , LEE, Jeong Wook , SONG, Hyohak
IPC分类号： C12N15/04 , C12N1/15
CPC分类号： C12P7/46 , C12N9/0006 , C12N9/1217
摘要： The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydrogenase-encoding gene ( idhA ) and an acetate kinase-encoding gene ( ackA ), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.
摘要翻译：本发明涉及以高产率产生高聚琥珀酸的微生物变异体，以及使用该方法生产同型琥珀酸的方法，更具体地说，涉及通过破坏编码乳酸脱氢酶基因（idhA）和乙酸盐激酶编码基因（ackA）以及高浓度均聚琥珀酸的方法，包括在厌氧条件下使用葡萄糖作为碳源培养这些变体。

5. 发明授权

EP2993225B1 NOVEL MUTANT MICROORGANISM PRODUCING SUCCINIC ACID SIMULTANEOUSLY USING SUCROSE AND GLYCEROL, AND METHOD FOR PREPARING SUCCINIC ACID USING SAME 有权
标题翻译：利用蔗糖和甘油同时产生琥珀酸的新型突变微生物产生琥珀酸及其使用相同方法制备琥珀酸的方法
公开(公告)号：EP2993225B1
公开(公告)日：2017-11-29
申请号：EP15188336.0
申请日：2011-08-30
申请人： Korea Advanced Institute of Science and Technology
发明人： Lee, Sang Yup , Choi, Sol , Yi, Jongho , LEE, Jeong Wook
IPC分类号： C12N1/21 , C12N15/52 , C12P7/40 , C12N1/20 , C12P7/46 , C12R1/01
CPC分类号： C12P7/46 , C12N1/20 , C12N15/52 , C12R1/01
摘要： The present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously as carbon sources. More particularly, the present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously for succinic acid production, the mutant organism being obtained by relieving the mechanism of sucrose-mediated catabolite repression of glycerol in a succinic acid-producing microorganism. As described above, when the succinic acid-producing mutant microorganism is cultured, it utilizes sucrose and glycerol simultaneously so that succinic acid can be produced with high productivity in a maximum yield approaching the theoretical yield while the production of byproducts is minimized. In addition, according to the present invention, various reduced chemicals which have been produced in low yields in conventional methods can be more effectively produced.

6. 发明公开

EP2612905A2 NOVEL MUTANT MICROORGANISM PRODUCING SUCCINIC ACID SIMULTANEOUSLY USING SUCROSE AND GLYCEROL, AND METHOD FOR PREPARING SUCCINIC ACID USING SAME 有权
标题翻译： NEW突变微生物琥珀酸与琥珀酸的生产，使蔗糖和甘油和方法的同时使用PRODUCTION
公开(公告)号：EP2612905A2
公开(公告)日：2013-07-10
申请号：EP11822105.0
申请日：2011-08-30
申请人： Korea Advanced Institute Of Science And Technology
发明人： LEE, Sang Yup , LEE, Jeong Wook , CHOI, Sol , YI, Jongho
IPC分类号： C12N1/21 , C12N15/52 , C12P7/40
CPC分类号： C12P7/46 , C12N1/20 , C12N15/52 , C12R1/01
摘要： The present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously as carbon sources. More particularly, the present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously for succinic acid production, the mutant organism being obtained by relieving the mechanism of sucrose-mediated catabolite repression of glycerol in a succinic acid-producing microorganism. As described above, when the succinic acid-producing mutant microorganism is cultured, it utilizes sucrose and glycerol simultaneously so that succinic acid can be produced with high productivity in a maximum yield approaching the theoretical yield while the production of byproducts is minimized. In addition, according to the present invention, various reduced chemicals which have been produced in low yields in conventional methods can be more effectively produced.
摘要翻译：本发明涉及一种产琥珀酸微生物突变体确实能够利用蔗糖和甘油同时作为碳源。更具体地，本发明涉及一种产琥珀酸微生物突变体也能够利用蔗糖和甘油同时用于琥珀酸生产，所述突变体有机体由缓和甘油的蔗糖介导的分解代谢物抑制的机制在琥珀酸获得 - 生产微生物。如上所述，当产生琥珀酸的突变体微生物进行培养时，它利用蔗糖和甘油同时所以也琥珀酸可以以高生产率在最大产率接近理论产率而生产的副产物最小化产生的。此外，雅鼎在本发明中，已在低产量被生产中的常规方法减少各种化学品可以更有效地生产。

7. 发明公开

EP2993225A3 NOVEL MUTANT MICROORGANISM PRODUCING SUCCINIC ACID SIMULTANEOUSLY USING SUCROSE AND GLYCEROL, AND METHOD FOR PREPARING SUCCINIC ACID USING SAME 有权
标题翻译：利用蔗糖和甘油同时产生琥珀酸的新型突变微生物产生琥珀酸及其使用相同方法制备琥珀酸的方法
公开(公告)号：EP2993225A3
公开(公告)日：2016-04-20
申请号：EP15188336.0
申请日：2011-08-30
申请人： Korea Advanced Institute of Science and Technology
发明人： Lee, Sang Yup , Choi, Sol , Yi, Jongho , LEE, Jeong Wook
IPC分类号： C12N1/21 , C12N15/52 , C12P7/40 , C12N1/20 , C12P7/46 , C12R1/01
CPC分类号： C12P7/46 , C12N1/20 , C12N15/52 , C12R1/01
摘要： The present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously as carbon sources. More particularly, the present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously for succinic acid production, the mutant organism being obtained by relieving the mechanism of sucrose-mediated catabolite repression of glycerol in a succinic acid-producing microorganism.
As described above, when the succinic acid-producing mutant microorganism is cultured, it utilizes sucrose and glycerol simultaneously so that succinic acid can be produced with high productivity in a maximum yield approaching the theoretical yield while the production of byproducts is minimized. In addition, according to the present invention, various reduced chemicals which have been produced in low yields in conventional methods can be more effectively produced.
摘要翻译：本发明涉及能够同时利用蔗糖和甘油作为碳源的产琥珀酸的突变微生物。更具体地说，本发明涉及能够同时利用蔗糖和甘油生产琥珀酸的琥珀酸生产突变体微生物，该突变生物体通过减轻琥珀酸中蔗糖介导的分解代谢物抑制甘油的机制而获得生产微生物。如上所述，当培养产琥珀酸的突变体微生物时，它同时利用蔗糖和甘油，从而可以高生产率生产琥珀酸，其最大产量接近理论产量，而副产物的产量最小化。另外，根据本发明，可以更有效地生产在常规方法中以低收率生产的各种还原化学品。

8. 发明公开

EP2546263A2 HIGH MOLECULAR WEIGHT RECOMBINANT SILK OR SILK-LIKE PROTEIN, AND MICRO- OR NANOSCALE SPIDER-WEB FIBER OR SPIDER-WEB-LIKE FIBER PRODUCED USING THE RECOMBINANT SILK OR SILK-LIKE PROTEIN 审中-公开
标题翻译：重组丝蛋白或组织样蛋白由高分子量，并从重组丝蛋白或像蛋白生产或超细纤维纳米级蜘蛛网和蜘蛛网状纤维
公开(公告)号：EP2546263A2
公开(公告)日：2013-01-16
申请号：EP11713459.3
申请日：2011-03-11
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , XIA, Xiaoxia , QIAN, Zhi Gang , LEE, Jeong Wook , PARK, Young Hwan
IPC分类号： C07K14/435 , C12N15/12 , C12N15/63 , D01F4/00
CPC分类号： C07K14/43518 , D01D5/0007 , D01D5/06 , D01F4/02
摘要： The present invention relates to a high-molecular-weight recombinant silk or silk-like protein having a molecular weight which is substantially similar to that of native silk protein, and to a micro- or nano-sized spider silk or silk-like fiber having improved physical properties, produced therefrom. The recombinant silk or silk-like protein according to the invention has high molecular weight, like dragline silk proteins from spiders, while a fiber produced therefrom has excellent physical properties compared to a fiber produced from native silk protein. Thus, the recombinant silk or silk-like protein and the spider silk or silk-like fiber produced therefrom will be highly useful in various industrial applications, including bioengineering applications and medical applications.
摘要翻译：本发明涉及一种高分子量的重组丝或丝样蛋白质基本上所有的分子量，其类似于做天然丝蛋白的，并以微米或纳米尺寸的蜘蛛丝或丝状纤维具有改进的物理性能，从那里产生的。重组丝或丝样蛋白gemäß到，本发明具有高的分子量，例如从蜘蛛拖丝蛋白质，而纤维产生有从具有优异的物理性能相比于从天然丝蛋白生产的纤维。因此，重组丝或丝样蛋白和蜘蛛丝或由其产生丝状纤维将是在各种工业应用中，包括生物工程应用和医疗应用中非常有用的。

9. 发明授权

EP2612905B1 NOVEL MUTANT MICROORGANISM PRODUCING SUCCINIC ACID SIMULTANEOUSLY USING SUCROSE AND GLYCEROL, AND METHOD FOR PREPARING SUCCINIC ACID USING SAME 有权
标题翻译： NEW突变微生物琥珀酸与琥珀酸的生产，使蔗糖和甘油和方法的同时使用PRODUCTION
公开(公告)号：EP2612905B1
公开(公告)日：2016-10-05
申请号：EP11822105.0
申请日：2011-08-30
申请人： Korea Advanced Institute Of Science And Technology
发明人： LEE, Sang Yup , LEE, Jeong Wook , CHOI, Sol , YI, Jongho
IPC分类号： C12N1/21 , C12N15/52 , C12P7/40 , C12N1/20 , C12P7/46 , C12R1/01
CPC分类号： C12P7/46 , C12N1/20 , C12N15/52 , C12R1/01

10. 发明公开

EP2993225A2 NOVEL MUTANT MICROORGANISM PRODUCING SUCCINIC ACID SIMULTANEOUSLY USING SUCROSE AND GLYCEROL, AND METHOD FOR PREPARING SUCCINIC ACID USING SAME 有权
标题翻译： NEW突变微生物琥珀酸与琥珀酸的生产，使蔗糖和方法的同时使用PRODUCTION
公开(公告)号：EP2993225A2
公开(公告)日：2016-03-09
申请号：EP15188336.0
申请日：2011-08-30
申请人： Korea Advanced Institute of Science and Technology
发明人： Lee, Sang Yup , Choi, Sol , Yi, Jongho , LEE, Jeong Wook
IPC分类号： C12N1/21 , C12N15/52 , C12P7/40
CPC分类号： C12P7/46 , C12N1/20 , C12N15/52 , C12R1/01
摘要： The present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously as carbon sources. More particularly, the present invention relates to a succinic acid-producing mutant microorganism that is able to utilize sucrose and glycerol simultaneously for succinic acid production, the mutant organism being obtained by relieving the mechanism of sucrose-mediated catabolite repression of glycerol in a succinic acid-producing microorganism.
As described above, when the succinic acid-producing mutant microorganism is cultured, it utilizes sucrose and glycerol simultaneously so that succinic acid can be produced with high productivity in a maximum yield approaching the theoretical yield while the production of byproducts is minimized. In addition, according to the present invention, various reduced chemicals which have been produced in low yields in conventional methods can be more effectively produced.
摘要翻译：本发明涉及一种产琥珀酸微生物突变体确实能够利用蔗糖和甘油同时作为碳源。更具体地，本发明涉及一种产琥珀酸微生物突变体也能够利用蔗糖和甘油同时用于琥珀酸生产，所述突变体有机体由缓和甘油的蔗糖介导的分解代谢物抑制的机制在琥珀酸获得 - 生产微生物。如上所述，当产生琥珀酸的突变体微生物进行培养时，它利用蔗糖和甘油同时所以也琥珀酸可以以高生产率在最大产率接近理论产率而生产的副产物最小化产生的。此外，雅鼎在本发明中，已在低产量被生产中的常规方法减少各种化学品可以更有效地生产。

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