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1. 发明申请

WO2005123924A1 METHOD FOR CELL SURFACE DISPLAY OF TARGET PROTEINS USING FADL OF E. COLI 审中-公开
标题翻译：使用大肠杆菌FADL的目标蛋白的细胞表面显示方法
公开(公告)号：WO2005123924A1
公开(公告)日：2005-12-29
申请号：PCT/KR2004/002181
申请日：2004-08-30
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Seung Hwan , PARK, Si Jae
发明人： LEE, Sang Yup , LEE, Seung Hwan , PARK, Si Jae
IPC分类号： C12N15/70
CPC分类号： C12N15/70
摘要： The present invention relates to an expression vector which can effectively express target proteins or peptides on the surface of cells using an outer membrane protein (FadL) of E. coli as a surface anchoring motif. Also, the present invention relates to microorganisms transformed with the expression vector, and a method for stably expressing large amounts of target proteins on the surface of cells by culturing the transformed microorganisms. Furthermore, the present invention relates to a production method of protein arrays, a production method of antibodies, and a bioconversion method, the methods being characterized by using target proteins which have been expressed on the cell surface by the inventive method. In addition, the present invention relates to a method for improving target proteins by the inventive surface expression method. The present invention allows target proteins with normal functions to be expressed on an outer cell membrane. Thus, the present invention will be useful in recombinant live vaccines, the screening of various peptides or antibodies, whole-cell adsorbents for heavy metal removal or waste water treatment, whole-cell bioconversion, and the like.
摘要翻译：本发明涉及使用大肠杆菌的外膜蛋白（FadL）作为表面锚定基序，能够在细胞表面上有效表达靶蛋白或肽的表达载体。此外，本发明涉及用表达载体转化的微生物，以及通过培养转化的微生物在细胞表面上稳定表达大量靶蛋白的方法。此外，本发明涉及蛋白质阵列的制造方法，抗体的制造方法以及生物转化方法，其特征在于，使用通过本发明的方法在细胞表面表达的靶蛋白。此外，本发明涉及通过本发明的表面表达方法改善靶蛋白的方法。本发明允许具有正常功能的靶蛋白在外细胞膜上表达。因此，本发明可用于重组活疫苗，各种肽或抗体的筛选，用于重金属去除或废水处理的全细胞吸附剂，全细胞生物转化等。

2. 发明申请

WO2004081202A1 METHOD FOR PRODUCING TARGET PROTEINS BY DELETING OR AMPLIFYINGibpA AND/OR ibpB GENE CODING FOR INCLUSION BODY-ASSOCIATED PROTEINS 审中-公开
标题翻译：通过去除或扩增ibpA和/或用于包含身体相关蛋白的ibpB基因编码来生产目标蛋白的方法
公开(公告)号：WO2004081202A1
公开(公告)日：2004-09-23
申请号：PCT/KR2003/001371
申请日：2003-07-10
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , HAN, Mee Jung , PARK, Si Jae
发明人： LEE, Sang Yup , HAN, Mee Jung , PARK, Si Jae
IPC分类号： C12N1/21
CPC分类号： C12P21/02 , C07K14/245 , C07K14/5434 , C07K14/57 , C07K14/65 , C12N15/70
摘要： The present invention relates to a method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. The present invention provides two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli , whose effect on the production of target proteins have not yet been reported. The first method of the present invention is to enhance the secretory production and activity of target proteins using ibpA and/or ibpB genes-deleted bacteria. The second method of the present invention is to enhance the production of target proteins in the cytoplasm and also convert the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.
摘要翻译：本发明涉及通过缺失或扩增编码包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的方法。本发明提供了使用编码大肠杆菌的包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的两种方法，其对靶蛋白的产生的影响尚未报道。本发明的第一种方法是使用ibpA和/或ibpB基因缺失的细菌增强靶蛋白的分泌生产和活性。本发明的第二种方法是使用ibpA和/或ibpB基因扩增的细菌，增强细胞质中靶蛋白的产生并将靶蛋白从可溶形式转化为不溶性包涵体。

3. 发明申请

WO2009031766A2 MUTANTS HAVING CAPABILITY TO PRODUCE 1,4-BUTANEDIOL AND METHOD FOR PREPARING 1,4-BUTANEDIOL USING THE SAME 审中-公开
标题翻译：具有生产1,4-丁二醇能力的突变体以及使用该方法制备1,4-丁二醇的方法
公开(公告)号：WO2009031766A2
公开(公告)日：2009-03-12
申请号：PCT/KR2008/004700
申请日：2008-08-13
申请人： LG CHEM, LTD. , KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , PARK, Si Jae , LEE, Sang Hyun , LEE, Sang Yup , LEE, Eun Jeong
发明人： PARK, Si Jae , LEE, Sang Hyun , LEE, Sang Yup , LEE, Eun Jeong
IPC分类号： C12N15/10
CPC分类号： C12N15/77 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/13 , C12P7/18 , C12Y101/01061 , C12Y102/01024 , C12Y103/99002
摘要： A mutant capable of producing 1,4-butanediol and a method of preparing 1,4- butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4- hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.
摘要翻译：提供了能够生产1,4-丁二醇的突变体和使用该突变体制备1,4-丁二醇的方法。通过在能够产生琥珀酸的微生物中引入和扩增编码将琥珀酸转化成4-羟基丁酸和4-羟基丁酸的酶的基因并扩增到1,4-丁二醇中来制备突变体微生物。该方法包括在含有碳水化合物的培养基中培养突变体并从培养物中获得1,4-丁二醇。因此，化学工业中必不可少的1,4-丁二醇可以用生物过程制备。

4. 发明申请

WO2005023847A1 COMPOSITION FOR PROTECTING PROTEINS DEGRADATION COMPRISING SMALL HEAT SHOCK PROTEINS (sHSPs) AND METHOD OF TWO-DIMENSIONAL GEL ELECTROPHORESIS USING THE sHSPs 审中-公开
标题翻译：用于保护包含小热休克蛋白（sHSP）的蛋白质降解的组合物和使用sHSP的两维凝胶电泳方法
公开(公告)号：WO2005023847A1
公开(公告)日：2005-03-17
申请号：PCT/KR2003/002539
申请日：2003-11-24
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , HAN, Mee Jung , PARK, Si Jae
发明人： LEE, Sang Yup , HAN, Mee Jung , PARK, Si Jae
IPC分类号： C07K14/00
CPC分类号： C07K14/195 , C07K14/00
摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional gel electrophoresis. Furthermore, the present invention relates to the improved method of two-dimensional gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the two-dimensional gel electrophoresis, thereby obtaining two-dimensional gel with much more protein spots.
摘要翻译：本发明涉及含有用于预防蛋白质降解的sHSP和用于二维凝胶电泳的组合物的组合物。此外，本发明涉及二维凝胶电泳的改进方法，其特征在于使用sHSP。根据本发明，在二维凝胶电泳中可以防止蛋白质斑点的减少，从而获得蛋白质斑点多的二维凝胶。

5. 发明申请

WO2009031762A2 RECOMBINANT MICROORGANISM CAPABLE OF PRODUCING POLYLACTATE OR POLYLACTATE COPOLYMER FROM SUCROSE AND METHOD FOR PRODUCING POLYLACTATE OR POLYLACTATE COPOLYMER FROM SUCROSE USING THE SAME 审中-公开
标题翻译：由SUCROSE生产聚合物或聚氨酯共聚物的重组微生物和使用其制备聚酯或聚氨酯共聚物的方法
公开(公告)号：WO2009031762A2
公开(公告)日：2009-03-12
申请号：PCT/KR2008/004611
申请日：2008-08-08
申请人： LG CHEM, LTD. , KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , PARK, Si Jae , KIM, Tae Wan , KANG, Hye Ok , YANG, Taek Ho , LEE, Sang Yup
发明人： PARK, Si Jae , KIM, Tae Wan , KANG, Hye Ok , YANG, Taek Ho , LEE, Sang Yup
IPC分类号： C12N15/05
CPC分类号： C12N15/8243 , C12N9/1025 , C12N15/8245 , C12P7/625
摘要： Provided are a microorganism capable of producing a polylactate (PLA) or PLA copolymer from sucrose and a method of producing the PLA or PLA copolymer from sucrose using the same. A gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate are introduced into a microorganism capable of using sucrose as a substrate, and the microorganism is cultured using sucrose as the substrate, thereby allowing efficient production of a PLA or PLA copolymer.
摘要翻译：提供能够从蔗糖生产聚乳酸（PLA）或PLA共聚物的微生物和使用其从蔗糖生产PLA或PLA共聚物的方法。将使用乳酰基辅酶A作为底物的将乳酸酯转化为乳酰基-CoA的酶基因和聚羟基链烷酸酯（PHA）合成酶的基因导入到能够使用蔗糖作为底物的微生物中，并使用蔗糖作为培养基从而能够有效地生产PLA或PLA共聚物。

6. 发明公开

EP2314638A2 METHOD FOR PREPARING POLYLACTATE AND COPOLYMER THEREOF USING A MUTANT MICROORGANISM WITH ENHANCED POLYLACTATE, AND THE COPOLYMER PRODUCING CAPABILITY THEREOF 审中-公开
标题翻译：用于生产聚乳酸及其共聚物通过改进聚乳酸与能力COPOLYMERERZEUGENDER突变的微生物手段
公开(公告)号：EP2314638A2
公开(公告)日：2011-04-27
申请号：EP09770374.8
申请日：2009-06-24
申请人： LG Chem, Ltd. , Korea Advanced Institute of Science and Technology
发明人： YANG, Taek Ho , PARK, Si Jae , KIM, Tae Wan , LEE, Sang Yup , JUNG, Yu Kyung
IPC分类号： C08G63/91
CPC分类号： C08G63/06 , C12P7/625 , C12P21/02
摘要： Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful.

7. 发明公开

EP3594350A1 METHOD FOR PRODUCING POLYHYDROXYALKANOATE USING 2-HYDROXYISOCAPROATE-COA TRANSFERASE 审中-实审
公开(公告)号：EP3594350A1
公开(公告)日：2020-01-15
申请号：EP18760488.9
申请日：2018-02-26
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , YANG, Jung Eun , PARK, Si Jae
IPC分类号： C12N15/63 , C12N9/10 , C12P7/62 , C08G63/688
摘要： The present invention relates to a recombinant microorganism to which a gene coding for 2-hydroxyisocaproate-CoA transferase and a gene coding for polyhydroxyalkanoate synthase are introduced and which has a potential of producing polyhydroxyalkanoate bearing an aromatic monomer or a long-chain 2-HA monomer and a method for producing polyhydroxyalkanoate bearing an aromatic monomer or a long-chain 2-HA monomer, using the recombinant microorganism. According to the present invention, a biodegradable polymer bearing an aromatic monomer or a long-chain 2-HA monomer can be produced.

8. 发明公开

EP1604015A1 METHOD FOR PRODUCING TARGET PROTEINS BY DELETING OR AMPLIFYING ibpA AND/OR ibpB GENE CODING FOR INCLUSION BODY-ASSOCIATED PROTEINS 有权
标题翻译：方法的靶蛋白借助于距离或者是身体相关蛋白编码IBPA AND / OR ibpB基因扩增夹杂物
公开(公告)号：EP1604015A1
公开(公告)日：2005-12-14
申请号：EP03816262.4
申请日：2003-07-10
申请人： Korea Advanced Institute of Science and Technology
发明人： LEE, Sang Yup , HAN, Mee Jung , PARK, Si Jae
IPC分类号： C12N1/21
CPC分类号： C12P21/02 , C07K14/245 , C07K14/5434 , C07K14/57 , C07K14/65 , C12N15/70
摘要： The present invention relates to a method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. The present invention provides two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli, whose effect on the production of target proteins have not yet been reported. The first method of the present invention is to enhance the secretory production and activity of target proteins using ibpA and/or ibpB genes-deleted bacteria. The second method of the present invention is to enhance the production of target proteins in the cytoplasm and also convert the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.

9. 发明授权

EP2684949B1 RECOMBINANT MICROORGANISM HAVING ABILITY TO PRODUCE [LACTATE-CO-GLYCOLATE]COPOLYMER FROM GLUCOSE, AND METHOD FOR PREPARING [LACTATE-CO-GLYCOLATE]COPOLYMER USING SAME 有权
标题翻译：具有从葡萄糖生产[乳酸酯 - 共 - 乙交酯]共聚物的能力的重组微生物以及使用相同方法制备[乳酸酯 - 共 - 乙醇酸酯]共聚物的方法
公开(公告)号：EP2684949B1
公开(公告)日：2018-05-02
申请号：EP12757647.8
申请日：2012-03-09
申请人： Korea Advanced Institute of Science and Technology , Korea Research Institute of Chemical Technology
发明人： LEE, Sang Yup , PARK, Si Jae , LEE, Seung Hwan , SONG, Bong Keun , JUNG, Yu Kyung , LEE, Tae Woo
IPC分类号： C12N1/21 , C12N15/52 , C12N15/31 , C12P7/40
CPC分类号： C12P7/625 , C12N9/0006 , C12N9/1029 , C12N15/70
摘要： There is provided a recombinant microorganism having producibility of poly(lactate-co-glycolate) from glucose, and more particularly, a recombinant microorganism having producibility of poly(lactate-co-glycolate) without adding an exogenous glycolate precursor, and a method of preparing [poly(preparing lactate-co-glycolate)] using the same. According to the present invention, the poly(lactate-co-glycolate) in which the concentration of the glycolate fraction is high may be prepared at a high concentration without supplying exogenous glyoxylate. Therefore, the present invention may be effectively used for treatment.

10. 发明公开

EP2684949A2 RECOMBINANT MICROORGANISM HAVING ABILITY TO PRODUCE [LACTATE-CO-GLYCOLATE]COPOLYMER FROM GLUCOSE, AND METHOD FOR PREPARING [LACTATE-CO-GLYCOLATE]COPOLYMER USING SAME 有权
标题翻译：需要有能力制备[LACTAT-CO-乙醇酸]葡萄糖共聚物，并产生[LACTAT-CO-乙醇酸]的方法的重组微生物共聚物SO
公开(公告)号：EP2684949A2
公开(公告)日：2014-01-15
申请号：EP12757647.8
申请日：2012-03-09
申请人： Korea Advanced Institute of Science and Technology , Korea Research Institute of Chemical Technology
发明人： LEE, Sang Yup , PARK, Si Jae , LEE, Seung Hwan , SONG, Bong Keun , JUNG, Yu Kyung , LEE, Tae Woo
IPC分类号： C12N1/21 , C12N15/52 , C12N15/31 , C12P7/40
CPC分类号： C12P7/625 , C12N9/0006 , C12N9/1029 , C12N15/70
摘要： There is provided a recombinant microorganism having producibility of poly(lactate-co-glycolate) from glucose, and more particularly, a recombinant microorganism having producibility of poly(lactate-co-glycolate) without adding an exogenous glycolate precursor, and a method of preparing [poly(preparing lactate-co-glycolate)] using the same. According to the present invention, the poly(lactate-co-glycolate) in which the concentration of the glycolate fraction is high may be prepared at a high concentration without supplying exogenous glyoxylate. Therefore, the present invention may be effectively used for treatment.
摘要翻译：提供了一种具有从葡萄糖聚（乳酸 - 共 - 乙醇酸）的可生产性，和重组微生物更特别地，具有聚（乳酸 - 共 - 乙醇酸）的可生产性，而不增加外源乙醇酸前体的重组微生物，以及制备的方法 [聚（乳酸制备 - 共 - 甘醇酸酯）]，用相同的。。根据本发明，聚（乳酸 - 共 - 乙醇酸），其中乙醇酸部分的浓度高，可以以高浓度没有提供外源乙醛酸制备。因此，本发明可以用于治疗有效。

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