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1. 发明申请

WO2004081202A1 METHOD FOR PRODUCING TARGET PROTEINS BY DELETING OR AMPLIFYINGibpA AND/OR ibpB GENE CODING FOR INCLUSION BODY-ASSOCIATED PROTEINS 审中-公开
标题翻译：通过去除或扩增ibpA和/或用于包含身体相关蛋白的ibpB基因编码来生产目标蛋白的方法
公开(公告)号：WO2004081202A1
公开(公告)日：2004-09-23
申请号：PCT/KR2003/001371
申请日：2003-07-10
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , HAN, Mee Jung , PARK, Si Jae
发明人： LEE, Sang Yup , HAN, Mee Jung , PARK, Si Jae
IPC分类号： C12N1/21
CPC分类号： C12P21/02 , C07K14/245 , C07K14/5434 , C07K14/57 , C07K14/65 , C12N15/70
摘要： The present invention relates to a method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. The present invention provides two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli , whose effect on the production of target proteins have not yet been reported. The first method of the present invention is to enhance the secretory production and activity of target proteins using ibpA and/or ibpB genes-deleted bacteria. The second method of the present invention is to enhance the production of target proteins in the cytoplasm and also convert the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.
摘要翻译：本发明涉及通过缺失或扩增编码包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的方法。本发明提供了使用编码大肠杆菌的包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的两种方法，其对靶蛋白的产生的影响尚未报道。本发明的第一种方法是使用ibpA和/或ibpB基因缺失的细菌增强靶蛋白的分泌生产和活性。本发明的第二种方法是使用ibpA和/或ibpB基因扩增的细菌，增强细胞质中靶蛋白的产生并将靶蛋白从可溶形式转化为不溶性包涵体。

2. 发明申请

WO2005023847A1 COMPOSITION FOR PROTECTING PROTEINS DEGRADATION COMPRISING SMALL HEAT SHOCK PROTEINS (sHSPs) AND METHOD OF TWO-DIMENSIONAL GEL ELECTROPHORESIS USING THE sHSPs 审中-公开
标题翻译：用于保护包含小热休克蛋白（sHSP）的蛋白质降解的组合物和使用sHSP的两维凝胶电泳方法
公开(公告)号：WO2005023847A1
公开(公告)日：2005-03-17
申请号：PCT/KR2003/002539
申请日：2003-11-24
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , HAN, Mee Jung , PARK, Si Jae
发明人： LEE, Sang Yup , HAN, Mee Jung , PARK, Si Jae
IPC分类号： C07K14/00
CPC分类号： C07K14/195 , C07K14/00
摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional gel electrophoresis. Furthermore, the present invention relates to the improved method of two-dimensional gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the two-dimensional gel electrophoresis, thereby obtaining two-dimensional gel with much more protein spots.
摘要翻译：本发明涉及含有用于预防蛋白质降解的sHSP和用于二维凝胶电泳的组合物的组合物。此外，本发明涉及二维凝胶电泳的改进方法，其特征在于使用sHSP。根据本发明，在二维凝胶电泳中可以防止蛋白质斑点的减少，从而获得蛋白质斑点多的二维凝胶。

3. 发明申请

WO2006126750A1 METHOD FOR PREPARING A TARGET PROTEIN USING THE sHSPs 审中-公开
标题翻译：使用sHSP制备目标蛋白的方法
公开(公告)号：WO2006126750A1
公开(公告)日：2006-11-30
申请号：PCT/KR2005/001500
申请日：2005-05-23
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , HAN, Mee Jung
发明人： LEE, Sang Yup , HAN, Mee Jung
IPC分类号： C07K1/14
CPC分类号： C07K1/14
摘要： The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs are added in cultivation, separation and purification processes for preparing a target protein, the target protein can be obtained at high yields by preventing the loss of protein by proteases. Also, when sHSPs are added in a reaction process using a whole cell enzyme or a partially purified enzyme, the yield of bioconversion using enzyme can be increased by preventing the loss of enzyme by proteases.
摘要翻译：本发明涉及用于分离和纯化靶蛋白的方法，制备靶蛋白的方法，以及由全细胞酶或部分纯化的酶进行生物转化的方法。根据本发明，为了制备目标蛋白质的培养，分离纯化方法添加sHSP，可以通过防止蛋白酶的蛋白质损失而以高收率获得目标蛋白质。此外，当使用全细胞酶或部分纯化的酶在反应过程中加入sHSP时，可以通过防止蛋白酶损失酶来增加使用酶的生物转化产率。

4. 发明申请

WO2009031766A2 MUTANTS HAVING CAPABILITY TO PRODUCE 1,4-BUTANEDIOL AND METHOD FOR PREPARING 1,4-BUTANEDIOL USING THE SAME 审中-公开
标题翻译：具有生产1,4-丁二醇能力的突变体以及使用该方法制备1,4-丁二醇的方法
公开(公告)号：WO2009031766A2
公开(公告)日：2009-03-12
申请号：PCT/KR2008/004700
申请日：2008-08-13
申请人： LG CHEM, LTD. , KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , PARK, Si Jae , LEE, Sang Hyun , LEE, Sang Yup , LEE, Eun Jeong
发明人： PARK, Si Jae , LEE, Sang Hyun , LEE, Sang Yup , LEE, Eun Jeong
IPC分类号： C12N15/10
CPC分类号： C12N15/77 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/13 , C12P7/18 , C12Y101/01061 , C12Y102/01024 , C12Y103/99002
摘要： A mutant capable of producing 1,4-butanediol and a method of preparing 1,4- butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4- hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.
摘要翻译：提供了能够生产1,4-丁二醇的突变体和使用该突变体制备1,4-丁二醇的方法。通过在能够产生琥珀酸的微生物中引入和扩增编码将琥珀酸转化成4-羟基丁酸和4-羟基丁酸的酶的基因并扩增到1,4-丁二醇中来制备突变体微生物。该方法包括在含有碳水化合物的培养基中培养突变体并从培养物中获得1,4-丁二醇。因此，化学工业中必不可少的1,4-丁二醇可以用生物过程制备。

5. 发明申请

WO2005123924A1 METHOD FOR CELL SURFACE DISPLAY OF TARGET PROTEINS USING FADL OF E. COLI 审中-公开
标题翻译：使用大肠杆菌FADL的目标蛋白的细胞表面显示方法
公开(公告)号：WO2005123924A1
公开(公告)日：2005-12-29
申请号：PCT/KR2004/002181
申请日：2004-08-30
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Seung Hwan , PARK, Si Jae
发明人： LEE, Sang Yup , LEE, Seung Hwan , PARK, Si Jae
IPC分类号： C12N15/70
CPC分类号： C12N15/70
摘要： The present invention relates to an expression vector which can effectively express target proteins or peptides on the surface of cells using an outer membrane protein (FadL) of E. coli as a surface anchoring motif. Also, the present invention relates to microorganisms transformed with the expression vector, and a method for stably expressing large amounts of target proteins on the surface of cells by culturing the transformed microorganisms. Furthermore, the present invention relates to a production method of protein arrays, a production method of antibodies, and a bioconversion method, the methods being characterized by using target proteins which have been expressed on the cell surface by the inventive method. In addition, the present invention relates to a method for improving target proteins by the inventive surface expression method. The present invention allows target proteins with normal functions to be expressed on an outer cell membrane. Thus, the present invention will be useful in recombinant live vaccines, the screening of various peptides or antibodies, whole-cell adsorbents for heavy metal removal or waste water treatment, whole-cell bioconversion, and the like.
摘要翻译：本发明涉及使用大肠杆菌的外膜蛋白（FadL）作为表面锚定基序，能够在细胞表面上有效表达靶蛋白或肽的表达载体。此外，本发明涉及用表达载体转化的微生物，以及通过培养转化的微生物在细胞表面上稳定表达大量靶蛋白的方法。此外，本发明涉及蛋白质阵列的制造方法，抗体的制造方法以及生物转化方法，其特征在于，使用通过本发明的方法在细胞表面表达的靶蛋白。此外，本发明涉及通过本发明的表面表达方法改善靶蛋白的方法。本发明允许具有正常功能的靶蛋白在外细胞膜上表达。因此，本发明可用于重组活疫苗，各种肽或抗体的筛选，用于重金属去除或废水处理的全细胞吸附剂，全细胞生物转化等。

6. 发明申请

WO2009031762A2 RECOMBINANT MICROORGANISM CAPABLE OF PRODUCING POLYLACTATE OR POLYLACTATE COPOLYMER FROM SUCROSE AND METHOD FOR PRODUCING POLYLACTATE OR POLYLACTATE COPOLYMER FROM SUCROSE USING THE SAME 审中-公开
标题翻译：由SUCROSE生产聚合物或聚氨酯共聚物的重组微生物和使用其制备聚酯或聚氨酯共聚物的方法
公开(公告)号：WO2009031762A2
公开(公告)日：2009-03-12
申请号：PCT/KR2008/004611
申请日：2008-08-08
申请人： LG CHEM, LTD. , KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , PARK, Si Jae , KIM, Tae Wan , KANG, Hye Ok , YANG, Taek Ho , LEE, Sang Yup
发明人： PARK, Si Jae , KIM, Tae Wan , KANG, Hye Ok , YANG, Taek Ho , LEE, Sang Yup
IPC分类号： C12N15/05
CPC分类号： C12N15/8243 , C12N9/1025 , C12N15/8245 , C12P7/625
摘要： Provided are a microorganism capable of producing a polylactate (PLA) or PLA copolymer from sucrose and a method of producing the PLA or PLA copolymer from sucrose using the same. A gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate are introduced into a microorganism capable of using sucrose as a substrate, and the microorganism is cultured using sucrose as the substrate, thereby allowing efficient production of a PLA or PLA copolymer.
摘要翻译：提供能够从蔗糖生产聚乳酸（PLA）或PLA共聚物的微生物和使用其从蔗糖生产PLA或PLA共聚物的方法。将使用乳酰基辅酶A作为底物的将乳酸酯转化为乳酰基-CoA的酶基因和聚羟基链烷酸酯（PHA）合成酶的基因导入到能够使用蔗糖作为底物的微生物中，并使用蔗糖作为培养基从而能够有效地生产PLA或PLA共聚物。

7. 发明申请

WO2009078687A3 RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE 审中-公开
公开(公告)号：WO2009078687A3
公开(公告)日：2009-06-25
申请号：PCT/KR2008/007533
申请日：2008-12-18
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
发明人： LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
IPC分类号： C12N9/10 , C12N9/26 , C12N15/09 , C12N15/00
摘要： The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.

8. 发明申请

WO2008088104A1 MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 审中-公开
标题翻译：具有改进的分支氨基酸生产能力的突变微生物及使用其制备分支氨基酸的方法
公开(公告)号：WO2008088104A1
公开(公告)日：2008-07-24
申请号：PCT/KR2007/001263
申请日：2007-03-14
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
发明人： LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
IPC分类号： C12N1/16
CPC分类号： C12P13/08 , C12N9/1014 , C12Y201/02011
摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site- specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.
摘要翻译：本发明涉及具有提高的支链氨基酸生产力的突变微生物，以及使用突变微生物生产支链氨基酸的方法。更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

9. 发明申请

WO2004061453A1 A PROTEIN CHIP FOR ANALYZING INTERACTION BETWEEN PROTEIN AND SUBSTRATE PEPTIDE THEREOF 审中-公开
标题翻译：蛋白质芯片，用于分析蛋白质与其底物之间的相互作用
公开(公告)号：WO2004061453A1
公开(公告)日：2004-07-22
申请号：PCT/KR2003/002183
申请日：2003-10-18
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Seok Jae
发明人： LEE, Sang Yup , LEE, Seok Jae
IPC分类号： G01N33/543
CPC分类号： C40B30/04 , C07K1/1077 , C07K14/5759 , C12N9/0006 , G01N33/54353 , G01N33/6803
摘要： The present invention relates to a protein chip of a S-L-SP form wherein a substrate peptide (SP) is immobilized on a solid substrate (S) by the mediation of a linker protein (L), as well as a method for analyzing the interaction between a reactive protein and its substrate peptide using such a protein chip. This analysis method for the interaction between a reactive protein and its substrate peptide using the protein chip comprises the steps of: adding a reactive protein to the protein chip, the reactive protein showing a specific interaction with the substrate protein immobilized on the protein chip; and detecting the interaction between the reactive protein and the substrate peptide. The present invention allows an increase in the reactivity between a peptide with low molecular weight and an enzyme with high molecular weight and between the peptide and a reactive antibody on the protein chip, so that the interaction between the peptide and the protein can be analyzed rapidly and massively.
摘要翻译：本发明涉及SL-SP形式的蛋白质芯片，其中通过连接蛋白（L）的介导将底物肽（SP）固定在固体底物（S）上，以及分析相互作用的方法在使用这种蛋白质芯片的反应蛋白和其底物肽之间。使用蛋白质芯片的反应蛋白与其底物肽之间的相互作用的分析方法包括以下步骤：向蛋白质芯片添加反应蛋白，反应蛋白与固定在蛋白质芯片上的底物蛋白显示特异性相互作用; 并检测反应蛋白和底物肽之间的相互作用。本发明允许增加低分子量肽和高分子量酶之间以及蛋白质芯片上的肽与反应性抗体之间的反应性，从而可以快速分析肽与蛋白质之间的相互作用并大量。

10. 发明申请

WO2009008574A1 HOMO-SUCCINIC ACID PRODUCING MICROORGANISM VARIANT AND PROCESS FOR PREPARING SUCCINIC ACID USING THE SAME 审中-公开
标题翻译：制备微生物的肝细胞生长因子及其制备方法
公开(公告)号：WO2009008574A1
公开(公告)日：2009-01-15
申请号：PCT/KR2008/000241
申请日：2008-01-15
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , KIM, Ji Mahn , LEE, Jeong Wook , SONG, Hyohak
发明人： LEE, Sang Yup , KIM, Ji Mahn , LEE, Jeong Wook , SONG, Hyohak
IPC分类号： C12N15/04 , C12N1/15
CPC分类号： C12P7/46 , C12N9/0006 , C12N9/1217
摘要： The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydrogenase-encoding gene ( idhA ) and an acetate kinase-encoding gene ( ackA ), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.
摘要翻译：本发明涉及以高产率产生高聚琥珀酸的微生物变异体，以及使用该方法生产同型琥珀酸的方法，更具体地说，涉及通过破坏编码乳酸脱氢酶基因（idhA）和乙酸盐激酶编码基因（ackA）以及高浓度均聚琥珀酸的方法，包括在厌氧条件下使用葡萄糖作为碳源培养这些变体。

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