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1. 发明授权

EP2684949B1 RECOMBINANT MICROORGANISM HAVING ABILITY TO PRODUCE [LACTATE-CO-GLYCOLATE]COPOLYMER FROM GLUCOSE, AND METHOD FOR PREPARING [LACTATE-CO-GLYCOLATE]COPOLYMER USING SAME 有权
标题翻译：具有从葡萄糖生产[乳酸酯 - 共 - 乙交酯]共聚物的能力的重组微生物以及使用相同方法制备[乳酸酯 - 共 - 乙醇酸酯]共聚物的方法
公开(公告)号：EP2684949B1
公开(公告)日：2018-05-02
申请号：EP12757647.8
申请日：2012-03-09
申请人： Korea Advanced Institute of Science and Technology , Korea Research Institute of Chemical Technology
发明人： LEE, Sang Yup , PARK, Si Jae , LEE, Seung Hwan , SONG, Bong Keun , JUNG, Yu Kyung , LEE, Tae Woo
IPC分类号： C12N1/21 , C12N15/52 , C12N15/31 , C12P7/40
CPC分类号： C12P7/625 , C12N9/0006 , C12N9/1029 , C12N15/70
摘要： There is provided a recombinant microorganism having producibility of poly(lactate-co-glycolate) from glucose, and more particularly, a recombinant microorganism having producibility of poly(lactate-co-glycolate) without adding an exogenous glycolate precursor, and a method of preparing [poly(preparing lactate-co-glycolate)] using the same. According to the present invention, the poly(lactate-co-glycolate) in which the concentration of the glycolate fraction is high may be prepared at a high concentration without supplying exogenous glyoxylate. Therefore, the present invention may be effectively used for treatment.

2. 发明公开

EP2684949A2 RECOMBINANT MICROORGANISM HAVING ABILITY TO PRODUCE [LACTATE-CO-GLYCOLATE]COPOLYMER FROM GLUCOSE, AND METHOD FOR PREPARING [LACTATE-CO-GLYCOLATE]COPOLYMER USING SAME 有权
标题翻译：需要有能力制备[LACTAT-CO-乙醇酸]葡萄糖共聚物，并产生[LACTAT-CO-乙醇酸]的方法的重组微生物共聚物SO
公开(公告)号：EP2684949A2
公开(公告)日：2014-01-15
申请号：EP12757647.8
申请日：2012-03-09
申请人： Korea Advanced Institute of Science and Technology , Korea Research Institute of Chemical Technology
发明人： LEE, Sang Yup , PARK, Si Jae , LEE, Seung Hwan , SONG, Bong Keun , JUNG, Yu Kyung , LEE, Tae Woo
IPC分类号： C12N1/21 , C12N15/52 , C12N15/31 , C12P7/40
CPC分类号： C12P7/625 , C12N9/0006 , C12N9/1029 , C12N15/70
摘要： There is provided a recombinant microorganism having producibility of poly(lactate-co-glycolate) from glucose, and more particularly, a recombinant microorganism having producibility of poly(lactate-co-glycolate) without adding an exogenous glycolate precursor, and a method of preparing [poly(preparing lactate-co-glycolate)] using the same. According to the present invention, the poly(lactate-co-glycolate) in which the concentration of the glycolate fraction is high may be prepared at a high concentration without supplying exogenous glyoxylate. Therefore, the present invention may be effectively used for treatment.
摘要翻译：提供了一种具有从葡萄糖聚（乳酸 - 共 - 乙醇酸）的可生产性，和重组微生物更特别地，具有聚（乳酸 - 共 - 乙醇酸）的可生产性，而不增加外源乙醇酸前体的重组微生物，以及制备的方法 [聚（乳酸制备 - 共 - 甘醇酸酯）]，用相同的。。根据本发明，聚（乳酸 - 共 - 乙醇酸），其中乙醇酸部分的浓度高，可以以高浓度没有提供外源乙醛酸制备。因此，本发明可以用于治疗有效。

3. 发明申请

WO2009078687A3 RECOMBINANT MICROORGANISM HAVING AN ABILITY OF USING SUCROSE AS A CARBON SOURCE 审中-公开
公开(公告)号：WO2009078687A3
公开(公告)日：2009-06-25
申请号：PCT/KR2008/007533
申请日：2008-12-18
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
发明人： LEE, Sang Yup , LEE, Jeong Wook , SONG, Hyohak , KIM, Ji Mahn , CHOI, Sol , PARK, Jin Hwan
IPC分类号： C12N9/10 , C12N9/26 , C12N15/09 , C12N15/00
摘要： The present invention relates to a recombinant microorganism capable of metabolizing sucrose, and more particularly to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding sucrose phosphotransferase and/or a gene encoding sucrose-6-phosphate hydrolase is introduced or to a recombinant microorganism capable of metabolizing sucrose in which a gene encoding β-fructofuranosidase is introduced. According to the present invention, a recombinant microorganism capable of using inexpensive sucrose as a carbon source instead of expensive glucose is provided. In addition, in a process of culturing microorganisms which have been incapable of using sucrose as a carbon source, sucrose can substitute for other carbon sources including glucose.

4. 发明申请

WO2008088104A1 MUTANT MICROORGANISM HAVING IMPROVED PRODUCTION ABILITY OF BRANCHED AMINO ACID AND METHOD FOR PREPARING BRANCHED AMINO ACID USING THE SAME 审中-公开
标题翻译：具有改进的分支氨基酸生产能力的突变微生物及使用其制备分支氨基酸的方法
公开(公告)号：WO2008088104A1
公开(公告)日：2008-07-24
申请号：PCT/KR2007/001263
申请日：2007-03-14
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
发明人： LEE, Sang Yup , PARK, Jin Hwan , LEE, Kwang Ho , KIM, Tae Yong
IPC分类号： C12N1/16
CPC分类号： C12P13/08 , C12N9/1014 , C12Y201/02011
摘要： The present invention relates to mutant microorganisms having improved productivity of branched-chain amino acids, and a method for producing branched-chain amino acids using the mutant microorganisms. More specifically, relates to mutant microorganisms having improved productivity of L-valine, which are produced by attenuating or deleting a gene encoding an enzyme involved in L-isoleucine biosynthesis, a gene encoding an enzyme involved in L-leucine, and a gene encoding an enzyme involved in D-pantothenic acid biosynthesis, and mutating a gene encoding an enzyme involved in L-valine biosynthesis, such that the expression thereof is increased, as well as a method for producing L-valine using the mutant microorganisms. The inventive mutant microorganisms produced by site- specific mutagenesis and metabolic pathway engineering can produce branched-chain amino acids, particularly L-valine, with high efficiency, and thus will be useful as industrial microorganisms for producing L-valine.
摘要翻译：本发明涉及具有提高的支链氨基酸生产力的突变微生物，以及使用突变微生物生产支链氨基酸的方法。更具体地，涉及通过减弱或缺失编码参与L-异亮氨酸生物合成的酶的基因，编码L-亮氨酸的酶的基因和编码L-亮氨酸的基因而产生的L-缬氨酸的生产率提高的突变体微生物涉及D-泛酸生物合成的酶，以及编码参与L-缬氨酸生物合成的酶的基因的突变，使得其表达增加，以及使用突变微生物产生L-缬氨酸的方法。通过位点特异性诱变和代谢途径工程产生的本发明的突变体微生物可以高效地产生支链氨基酸，特别是L-缬氨酸，因此可用作生产L-缬氨酸的工业微生物。

5. 发明申请

WO2006126750A1 METHOD FOR PREPARING A TARGET PROTEIN USING THE sHSPs 审中-公开
标题翻译：使用sHSP制备目标蛋白的方法
公开(公告)号：WO2006126750A1
公开(公告)日：2006-11-30
申请号：PCT/KR2005/001500
申请日：2005-05-23
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , HAN, Mee Jung
发明人： LEE, Sang Yup , HAN, Mee Jung
IPC分类号： C07K1/14
CPC分类号： C07K1/14
摘要： The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs are added in cultivation, separation and purification processes for preparing a target protein, the target protein can be obtained at high yields by preventing the loss of protein by proteases. Also, when sHSPs are added in a reaction process using a whole cell enzyme or a partially purified enzyme, the yield of bioconversion using enzyme can be increased by preventing the loss of enzyme by proteases.
摘要翻译：本发明涉及用于分离和纯化靶蛋白的方法，制备靶蛋白的方法，以及由全细胞酶或部分纯化的酶进行生物转化的方法。根据本发明，为了制备目标蛋白质的培养，分离纯化方法添加sHSP，可以通过防止蛋白酶的蛋白质损失而以高收率获得目标蛋白质。此外，当使用全细胞酶或部分纯化的酶在反应过程中加入sHSP时，可以通过防止蛋白酶损失酶来增加使用酶的生物转化产率。

6. 发明申请

WO2004061453A1 A PROTEIN CHIP FOR ANALYZING INTERACTION BETWEEN PROTEIN AND SUBSTRATE PEPTIDE THEREOF 审中-公开
标题翻译：蛋白质芯片，用于分析蛋白质与其底物之间的相互作用
公开(公告)号：WO2004061453A1
公开(公告)日：2004-07-22
申请号：PCT/KR2003/002183
申请日：2003-10-18
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Seok Jae
发明人： LEE, Sang Yup , LEE, Seok Jae
IPC分类号： G01N33/543
CPC分类号： C40B30/04 , C07K1/1077 , C07K14/5759 , C12N9/0006 , G01N33/54353 , G01N33/6803
摘要： The present invention relates to a protein chip of a S-L-SP form wherein a substrate peptide (SP) is immobilized on a solid substrate (S) by the mediation of a linker protein (L), as well as a method for analyzing the interaction between a reactive protein and its substrate peptide using such a protein chip. This analysis method for the interaction between a reactive protein and its substrate peptide using the protein chip comprises the steps of: adding a reactive protein to the protein chip, the reactive protein showing a specific interaction with the substrate protein immobilized on the protein chip; and detecting the interaction between the reactive protein and the substrate peptide. The present invention allows an increase in the reactivity between a peptide with low molecular weight and an enzyme with high molecular weight and between the peptide and a reactive antibody on the protein chip, so that the interaction between the peptide and the protein can be analyzed rapidly and massively.
摘要翻译：本发明涉及SL-SP形式的蛋白质芯片，其中通过连接蛋白（L）的介导将底物肽（SP）固定在固体底物（S）上，以及分析相互作用的方法在使用这种蛋白质芯片的反应蛋白和其底物肽之间。使用蛋白质芯片的反应蛋白与其底物肽之间的相互作用的分析方法包括以下步骤：向蛋白质芯片添加反应蛋白，反应蛋白与固定在蛋白质芯片上的底物蛋白显示特异性相互作用; 并检测反应蛋白和底物肽之间的相互作用。本发明允许增加低分子量肽和高分子量酶之间以及蛋白质芯片上的肽与反应性抗体之间的反应性，从而可以快速分析肽与蛋白质之间的相互作用并大量。

7. 发明申请

WO2009008574A1 HOMO-SUCCINIC ACID PRODUCING MICROORGANISM VARIANT AND PROCESS FOR PREPARING SUCCINIC ACID USING THE SAME 审中-公开
标题翻译：制备微生物的肝细胞生长因子及其制备方法
公开(公告)号：WO2009008574A1
公开(公告)日：2009-01-15
申请号：PCT/KR2008/000241
申请日：2008-01-15
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , KIM, Ji Mahn , LEE, Jeong Wook , SONG, Hyohak
发明人： LEE, Sang Yup , KIM, Ji Mahn , LEE, Jeong Wook , SONG, Hyohak
IPC分类号： C12N15/04 , C12N1/15
CPC分类号： C12P7/46 , C12N9/0006 , C12N9/1217
摘要： The present invention relates to microbial variants producing homo-succinic acid at high yields and a method for producing homo-succinic acid using the same, more particularly, to a microbial variant constructed by disrupting a lactate dehydrogenase-encoding gene ( idhA ) and an acetate kinase-encoding gene ( ackA ), as well as a method for producing homo-succinic acid at high concentration, which comprises culturing such variants using glucose as a carbon source in anaerobic conditions.
摘要翻译：本发明涉及以高产率产生高聚琥珀酸的微生物变异体，以及使用该方法生产同型琥珀酸的方法，更具体地说，涉及通过破坏编码乳酸脱氢酶基因（idhA）和乙酸盐激酶编码基因（ackA）以及高浓度均聚琥珀酸的方法，包括在厌氧条件下使用葡萄糖作为碳源培养这些变体。

8. 发明申请

WO2008111708A1 L-THREONINE OVERPRODUCING MICROORGANISM AND METHOD FOR PREPARING L-THREONINE USING THE SAME 审中-公开
标题翻译： L-THREONINE OVERPRODUCTION MICROORGANISM AND METHOD FOR PRIPARING L-THREONINE WITH THE SAME
公开(公告)号：WO2008111708A1
公开(公告)日：2008-09-18
申请号：PCT/KR2007/003200
申请日：2007-07-02
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , LEE, Kwang Ho , PARK, Jin Hwan , KIM, Tae Yong
发明人： LEE, Sang Yup , LEE, Kwang Ho , PARK, Jin Hwan , KIM, Tae Yong
IPC分类号： C12N15/09 , C12N1/21 , C12P13/08
CPC分类号： C12P13/08
摘要： The present invention relates to a mutant microorganism producing a high concentration of L-threonine in high yield, prepared using site-specific mutation, not random mutation, such as treatment with a mutation inducer, a method for preparing the same, and a method for preparing L-threonine using the mutant microorganism producing L-threonine. By using the mutant microorganism according to the present invention, L-threonine can be prepared at high yield, additional strain development becomes possible and their physiological phenomena can be easily understood since genetic information of L-threonine producing microorganism can be identified.
摘要翻译：本发明涉及使用位点特异性突变而不是任意突变（例如用突变诱导剂处理）制备高产量的L-苏氨酸的高浓度的突变型微生物，其制备方法和使用产生L-苏氨酸的突变体微生物制备L-苏氨酸。通过使用根据本发明的突变微生物，可以高产率制备L-苏氨酸，由于可以鉴定L-苏氨酸生产微生物的遗传信息，所以可以进行附加应变发展，并且可以容易地理解其生理现象。

9. 发明申请

WO2008078911A1 METHOD FOR SCREENING ESSENTIAL METABOLITES IN GROWTH OF MICROORGANISMS 审中-公开
标题翻译：筛选微生物生长中基本代谢物的方法
公开(公告)号：WO2008078911A1
公开(公告)日：2008-07-03
申请号：PCT/KR2007/006708
申请日：2007-12-21
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , JEONG, Hawoong , KIM, Tae Yong , KIM, Pan-Jun , LEE, Kwang Ho
发明人： LEE, Sang Yup , JEONG, Hawoong , KIM, Tae Yong , KIM, Pan-Jun , LEE, Kwang Ho
IPC分类号： G01N33/48
CPC分类号： C12Q1/18 , G01N33/5038
摘要： The present invention disclosed is a method for screening metabolites essential for the growth of microorganism using metabolic flux analysis. More specifically, the present invention relates to the method for screening metabolites essential for the growth of microorganism, by selecting a target microorganism, constructing a metabolic network model of the selected microorganism, inactivating the consumption reaction of each of metabolites in the constructed metabolic network model, analyzing the metabolic flux of the metabolites to select metabolites essential for the growth of the microorganism, and confirming the selected metabolites using the utilization of each of the metabolites, defined as flux sum (Φ ). According to the present invention, metabolites essential for the growth of microorganism, and genes involved in the essential metabolites, can be screened in a convenient manner, and drug-target genes against pathogenic microorganisms can be predicted by deleting genes associated with the metabolites screened according to the method.
摘要翻译：所公开的本发明是使用代谢通量分析来筛选微生物生长所必需的代谢物的方法。更具体地说，本发明涉及通过选择靶微生物，构建所选择的微生物的代谢网络模型，在构建的代谢网络模型中灭活各代谢物的消耗反应来筛选微生物生长所必需的代谢物的方法分析代谢物的代谢通量以选择对微生物生长至关重要的代谢物，并使用定义为通量（F）的每种代谢物来确认所选择的代谢物。根据本发明，可以以便利的方式筛选微生物生长所必需的代谢物和基本代谢物所涉及的基因，并且可以通过删除与代谢物相关的基因来预测针对病原微生物的药物靶基因的方法。

10. 发明申请

WO2006107127A1 METHOD FOR IMPROVING A STRAIN BASED ON IN-SILICO ANALYSIS 审中-公开
标题翻译：用于改进基于硅分析的应变的方法
公开(公告)号：WO2006107127A1
公开(公告)日：2006-10-12
申请号：PCT/KR2005/001501
申请日：2005-05-23
申请人： KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY , LEE, Sang Yup , KIM, Tae Yong , LEE, Dong Yup , LEE, Sang Jun
发明人： LEE, Sang Yup , KIM, Tae Yong , LEE, Dong Yup , LEE, Sang Jun
IPC分类号： C12N1/00
CPC分类号： C12P7/46 , C12N9/1205 , C12N15/1089
摘要： The present invention is related to a method for improving a strain on the basis of in silico analysis, in which it compares the genomic information of a target strain for producing a useful substance to the genomic information of a strain overproducing the useful substance so as to primarily screen genes unnecessary for the overproduction of the useful substance, and then to secondarily screen genes to be deleted through performing simulation with metabolic flux analysis. According to the present invention, an improved strain can be effectively constructed by the metabolic and genetic engineering approach comprising comparatively analyzing the genomic information of a target strain for producing a useful substance and the genomic information of a strain producing a large amount of the useful substance to screen candidate genes and performing in silico simulation on the screened candidate genes to select a combination of genes to be deleted, which shows an improvement in the production of the useful substance. Accordingly, the time, effort and cost required for an actual wet test can be significantly reduced.
摘要翻译：本发明涉及一种基于在计算机分析中改进菌株的方法，其中将用于产生有用物质的靶菌株的基因组信息与过量产生有用物质的菌株的基因组信息进行比较，以便主要是用于过量生产有用物质的筛选基因，然后通过用代谢通量分析进行模拟来次要筛选待缺失的基因。根据本发明，可以通过代谢和基因工程方法有效地构建改进的菌株，其包括比较分析用于产生有用物质的靶菌株的基因组信息和产生大量有用物质的菌株的基因组信息筛选候选基因并在筛选的候选基因的计算机模拟中进行选择要缺失的基因的组合，其显示有用物质的生产的改善。因此，可以显着降低实际湿测试所需的时间，精力和成本。

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