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1. 发明授权

US08520280B2 Method and apparatus for dynamically shifting a light beam with regard to an optic focussing the light beam 有权
标题翻译：相对于聚焦光束的光学器件动态地移动光束的方法和装置
公开(公告)号：US08520280B2
公开(公告)日：2013-08-27
申请号：US13162055
申请日：2011-06-16
申请人： Johann Engelhardt
发明人： Johann Engelhardt
IPC分类号： G02B26/08
CPC分类号： G02B21/0048 , G02B26/101
摘要： For dynamically shifting a light beam (7) with regard to an optic focussing the light beam to scan an object in a two-dimensional scanning range with the focussed light beam, at least two beam deflectors (26) are connected in series per each direction in which the light beam is to be deflected with regard to the optical axis of the focussing optic. The two beam deflectors deflect the light beam by two deflection angles (31, 32 and 33, 34, respectively) which are dynamically variable independently on each other. The deflection angles (31 to 34) of all beam deflectors (26) are predetermined for each point of the scanning range (35) in such a way that the light beam (7), in scanning the whole two-dimensional scanning range, always runs through the pupil of the optic (4) at essentially the same point.
摘要翻译：为了相对于聚焦光束的光束来动态移动光束（7），所述光束以聚焦光束在二维扫描范围内扫描对象，至少两个光束偏转器（26）每个方向串联连接其中光束将相对于聚焦光学器件的光轴偏转。两个光束偏转器将光束偏转两个彼此独立地动态变化的偏转角（31,32和33,34）。所有光束偏转器（26）的偏转角（31至34）对于扫描范围（35）的每个点是预定的，使得光束（7）在扫描整个二维扫描范围时始终在基本相同的点处穿过光学元件（4）的光瞳。

2. 发明授权

US07428104B2 Optical device for the combination of light beams 有权
标题翻译：用于光束组合的光学装置
公开(公告)号：US07428104B2
公开(公告)日：2008-09-23
申请号：US10521892
申请日：2003-06-13
申请人： Johann Engelhardt
发明人： Johann Engelhardt
IPC分类号： G02B27/12
CPC分类号： G02B27/145 , G02B21/002 , G02B26/0875 , G02B27/123
摘要： An optical device for combining a first light beam and at least one second light beam includes a first beam splitting device, a second beam splitting device and a position detector. The first beam splitting device splits a first reference beam from the first light beam and a second reference beam from the second light beam. The second beam splitting device splits a third reference beam from the first light beam and a fourth reference beam from the second light beam. The position detector detects respective positions of the reference beams so as to enable a respective propagation direction and/or a respective position of the first and/or second light beams to be adjusted as a function of the detected positions of the reference beams.
摘要翻译：用于组合第一光束和至少一个第二光束的光学装置包括第一分束装置，第二分束装置和位置检测器。第一分束装置分离来自第一光束的第一参考光束和来自第二光束的第二参考光束。第二分束装置将来自第一光束的第三参考光束和来自第二光束的第四参考光束分离。位置检测器检测参考光束的各个位置，以使得能够根据检测到的参考光束的位置来调节第一和/或第二光束的相应传播方向和/或相应位置。

3. 发明授权

US07110645B2 Method and instrument for microscopy 有权
标题翻译：显微镜法和仪器
公开(公告)号：US07110645B2
公开(公告)日：2006-09-19
申请号：US10964034
申请日：2004-10-13
申请人： Holger Birk , Rafael Storz , Johann Engelhardt , Kyra Moellmann
发明人： Holger Birk , Rafael Storz , Johann Engelhardt , Kyra Moellmann
IPC分类号： G02B6/06 , G02B21/00
CPC分类号： G02B6/02376 , B82Y20/00 , G02B6/02366 , G02B6/02371 , G02B6/1225 , G02B6/2552 , G02B21/002 , G02B21/0024 , G02B21/0032 , G02B21/0056 , G02B21/0064 , G02B21/0068 , G02B21/0076 , G02B21/008 , G02B21/06 , H01S3/005 , H01S3/1625 , H01S3/1636 , Y10S385/901
摘要： A microscope includes a light source that emits an illuminating light beam for illumination of a specimen, a beam splitter separating measuring light out of the illuminating light beam, and an apparatus for determining the light power level of the illuminating light beam. The apparatus for determining the light power level of the illuminating light beam receives the measuring light and includes an apparatus for simultaneous color-selective detection of the measuring light.
摘要翻译：显微镜包括发射用于照射样本的照明光束的光源，分离出照射光束的测量光的分束器和用于确定照明光束的光功率水平的装置。用于确定照明光束的光功率电平的装置接收测量光，并且包括用于同时对测量光进行颜色选择性检测的装置。

4. 发明授权

US07054062B2 Double confocal scanning microscope 有权
标题翻译：双共焦扫描显微镜
公开(公告)号：US07054062B2
公开(公告)日：2006-05-30
申请号：US09826712
申请日：2001-04-05
申请人： Johann Engelhardt , Jörg Bewersdorf , Hilmar Gugel , Juergen Hoffmann
发明人： Johann Engelhardt , Jörg Bewersdorf , Hilmar Gugel , Juergen Hoffmann
IPC分类号： G02B21/06 , G02B21/00
CPC分类号： G02B21/0032 , G02B21/0056 , G02B21/0072 , G02B21/0076
摘要： The present invention concerns a double confocal scanning microscope (1) having an illuminating beam path (2) of at least one light source (3), and a detected beam path (4) of at least one detector (5), and in order to achieve almost the theoretically possible resolution capability, in particular in the context of multi-color fluorescence applications, is characterized in that the optical properties in particular of the components (6, 10, 13, 14) arranged in the beam path are coordinated with one another in such a way that the accumulated aberrations, with respect to the optical axis (33) and/or at least one surface (18, 19, 20) in the specimen region, are at least of the order of magnitude of the theoretically achievable resolution capability.
摘要翻译：本发明涉及具有至少一个光源（3）的照明光束路径（2）和至少一个检测器（5）的检测光束路径（4）的双共聚焦扫描显微镜（1），并且按顺序为了实现几乎理论上可能的分辨能力，特别是在多色荧光应用的上下文中，其特征在于布置在光束路径中的特别是组件（6,10,13,14）的光学特性与彼此相对于样本区域中相对于光轴（33）和/或至少一个表面（18,19,20）的累积像差至少在理论上的数量级上可实现的分辨能力。

5. 发明申请

US20060109547A1 Adjustment of a microscope 有权
标题翻译：调整显微镜
公开(公告)号：US20060109547A1
公开(公告)日：2006-05-25
申请号：US11327783
申请日：2006-01-06
申请人： Johann Engelhardt
发明人： Johann Engelhardt
IPC分类号： G02B21/00 , G02B21/06
CPC分类号： G02B21/241 , G02B21/18
摘要： In adjusting a microscope having at least two objectives for superimposing measuring beams of light in an object space to the end of obtaining an interference pattern and for monitoring the object space, each objective having a focal point, a focal plane and a pupil, auxiliary beams of light which are distinguishable from the measuring beams are directed into the objectives, and the auxiliary beams getting back out of the objectives are superimposed to obtain an auxiliary interference pattern. Further, the auxiliary beams getting back out of the objectives are imaged as spots.
摘要翻译：在调整具有至少两个物镜的显微镜的情况下，将物镜空间中的测量光束叠加到获得干涉图案的末端并用于监视物体空间，每个物镜具有焦点，焦平面和瞳孔，辅助光束与测量光束区分开的光被引导到物镜中，并且重叠回目标的辅助光束被叠加以获得辅助干涉图案。此外，从目标回退的辅助光束成像为点。

6. 发明授权

US07018042B2 Scanning microscope having an optical component, and optical component 失效
标题翻译：具有光学部件的扫描显微镜和光学部件
公开(公告)号：US07018042B2
公开(公告)日：2006-03-28
申请号：US10618345
申请日：2003-07-11
申请人： Johann Engelhardt
发明人： Johann Engelhardt
IPC分类号： G02B27/14
CPC分类号： G02B21/0056 , G02B21/0032 , G02B21/0064
摘要： An optical component is arranged in the beam path of a scanning microscope. The optical component has a plane entrance surface through which a light beam bundle can be incoupled at an entrance angle, and a plane exit surface through which the light beam bundle can be outcoupled at an exit angle, which is different from the entrance angle. The optical component contains at least two elements that exhibit at least two different refractive indices.
摘要翻译：光学部件布置在扫描显微镜的光束路径中。光学部件具有平面入射面，通过该平面入射面，光束束可以以入射角被耦合，并且平面出射面，光束束可以以与入射角不同的出射角被外耦合出来。光学部件包含至少两个表现出至少两个不同折射率的元件。

7. 发明授权

US06977773B2 Scanning microscope and coupling-out element 有权
标题翻译：扫描显微镜和耦合元件
公开(公告)号：US06977773B2
公开(公告)日：2005-12-20
申请号：US10766260
申请日：2004-01-27
申请人： Johann Engelhardt , Werner Knebel , Heinrich Ulrich
发明人： Johann Engelhardt , Werner Knebel , Heinrich Ulrich
IPC分类号： G02B21/00
CPC分类号： G02B21/008 , G02B21/0032
摘要： A scanning microscope has a light source that emits illuminating light for illumination of a specimen, at least one first detector for detection of the detected light proceeding from the specimen, an objective arranged in both an illumination beam path and a detection beam path, and a coupling-out element that is selectably for descan detection and non-descan detection positionable in the illumination and detection beam path. A light-guiding fiber is provided for transporting at least a portion of the detection light from the coupling-out element to the first detector.
摘要翻译：扫描显微镜具有发射用于照射样本的照明光的光源，用于检测从样本进行的检测光的至少一个第一检测器，布置在照明光束路径和检测光束路径中的物镜，以及耦合输出元件，其可选择地用于在照明和检测光束路径中定位的分辨率检测和非降低检测。提供了一种导光纤维，用于将来自耦合输出元件的检测光的至少一部分传输到第一检测器。

8. 发明授权

US06958858B2 Method for scanning microscopy; and scanning microscope 有权
标题翻译：扫描显微镜的方法; 和扫描显微镜
公开(公告)号：US06958858B2
公开(公告)日：2005-10-25
申请号：US10207334
申请日：2002-07-29
申请人： Johann Engelhardt , Juergen Hoffmann
发明人： Johann Engelhardt , Juergen Hoffmann
IPC分类号： G02B21/00 , G02B21/06 , G01J3/30
CPC分类号： G02B21/06 , G02B21/002
摘要： A method for scanning microscopy is disclosed. It contains the step of generating an illuminating light beam that exhibits at least a first substantially continuous wavelength spectrum whose spectral width is greater than 5 nm; the choosing of a second wavelength spectrum that is arranged spectrally within the first wavelength spectrum; the step of selecting the light of the second wavelength spectrum out of the illuminating light beam using an acoustooptical component; and the step of illuminating a specimen with the illuminating light beam. A scanning microscope is also disclosed.
摘要翻译：公开了扫描显微术的方法。它包括产生照明光束的步骤，其展现出至少第一基本连续的波长谱，其光谱宽度大于5nm; 选择在第一波长光谱内频谱排列的第二波长光谱; 使用声光分量从照明光束中选择第二波长光谱的光的步骤; 以及利用照明光束照射样本的步骤。还公开了扫描显微镜。

9. 发明授权

US06909540B2 Microscope objective, microscope, and method for imaging a specimen 有权
标题翻译：显微镜目标，显微镜和成像成像方法
公开(公告)号：US06909540B2
公开(公告)日：2005-06-21
申请号：US10308608
申请日：2002-12-02
申请人： Johann Engelhardt , Rafael Storz
发明人： Johann Engelhardt , Rafael Storz
IPC分类号： G02B7/04 , G02B21/00 , G02B21/02 , G02B21/24
CPC分类号： G02B21/02 , G02B21/241
摘要： A microscope objective that comprises an objective housing and contains several lens elements, at least one lens element being arranged displaceably in motor-driven fashion within the objective housing, is disclosed. A microscope and a method for imaging a specimen are additionally disclosed.
摘要翻译：公开了一种显微镜物镜，其包括物镜壳体并且包含若干透镜元件，至少一个透镜元件以电机驱动的方式在物镜壳体内可移位地布置。另外公开了用于成像试样的显微镜和方法。

10. 发明授权

US06710337B2 Laser scanning microscope and shutter for an optical system 有权
标题翻译：激光扫描显微镜和光学系统快门
公开(公告)号：US06710337B2
公开(公告)日：2004-03-23
申请号：US09884625
申请日：2001-06-19
申请人： Johann Engelhardt , William C. Hay , Juergen Hoffmann
发明人： Johann Engelhardt , William C. Hay , Juergen Hoffmann
IPC分类号： G21K700
CPC分类号： G02B21/002
摘要： An optical arrangement, in particular a laser scanning microscope, having a light source, in particular a laser light source, and an interruption device (1) for a light beam (2) of the light source, is configured, in the interest of reliable operation, in such a way that means (3) for monitoring the functioning of the interruption device (1) are associated with the interruption device (1). The invention additionally concerns a shutter (5) for a light beam (2) of a light source, in particular a laser light source, which, again in the interest of reliable operation of an optical arrangement, is characterized by at least two movable components (6, 7) which are configured and arranged such that the mechanical momentum generated by a moving component (6) or by several moving components is compensated for by the motion of the other component (7) or components.
摘要翻译：具有光源，特别是激光光源的光学装置，特别是激光扫描显微镜，以及用于光源的光束（2）的中断装置（1）被配置为有可能以这种方式，用于监视中断装置（1）的功能的装置（3）与中断装置（1）相关联。本发明还涉及用于光源的光束（2）的快门（5），特别是激光光源，其再次为了光学装置的可靠操作而特征在于至少两个可移动部件（6,7），其被构造和布置成使得由运动部件（6）产生的机械动量或由多个运动部件产生的机械动量通过另一部件（7）或部件的运动来补偿。

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