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1. 发明授权

US07153654B2 Assay procedure using fluorogenic tracers 失效
公开(公告)号：US07153654B2
公开(公告)日：2006-12-26
申请号：US10286600
申请日：2002-10-31
申请人： Ai-Ping Wei , James N. Herron
发明人： Ai-Ping Wei , James N. Herron
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02
CPC分类号： G01N33/582 , C12Q1/6818
摘要： Fluorescent energy transfer dyes capable of moving between a more stacked configuration to exhibit fluorescent quenching and a more spaced configuration to exhibit fluorescence can be conjugated to a peptide epitope or nucleic acid for use in the detection of an unknown antibody in bulk solution. The resulting labeled peptide reagent can be used in an immunoassay procedure by placing it in bulk solution along with the unknown antibody to be detected. When the antibody binds to the peptide epitope, the pair of dyes carried by the peptide epitope will have their configuration altered from a stacked to an unstacked configuration and will exhibit a fluorescent increase in response thereto.

2. 发明授权

US06576419B1 Assay procedure using fluorogenic tracers 失效
标题翻译：使用荧光示踪剂的测定程序
公开(公告)号：US06576419B1
公开(公告)日：2003-06-10
申请号：US08891114
申请日：1997-07-10
申请人： Ai-Ping Wei , James N. Herron
发明人： Ai-Ping Wei , James N. Herron
IPC分类号： C12Q108
CPC分类号： G01N33/582 , C12Q1/6818
摘要： Fluorescent energy transfer dyes capable of moving between a more stacked configuration to exhibit fluorescent quenching and a more spaced configuration to exhibit fluorescence can be conjugated to a peptide epitope or nucleic acid for use in the detection of an unknown antibody in bulk solution. The resulting labeled peptide reagent can be used in an immunoassay procedure by placing it in bulk solution along with the unknown antibody to be detected. When the antibody binds to the peptide epitope, the pair of dyes carried by the peptide epitope will have their configuration altered from a stacked to an unstacked configuration and will exhibit a fluorescent increase in response thereto.
摘要翻译：能够在更叠层的配置之间移动以展现荧光猝灭和更间隔的配置以显示荧光的荧光能量转移染料可以缀合到肽表位或核酸上，以用于检测本体溶液中的未知抗体。所得标记的肽试剂可以在免疫测定程序中使用，将其与待检测的未知抗体一起放入本体溶液中。当抗体与肽表位结合时，由肽表位携带的一对染料将具有从堆叠到未堆叠构型的改变，并且对其反应将呈现荧光增加。

3. 发明授权

US06632613B1 Compositions and kits for fluorescence polarization assay of large molecules 失效
标题翻译：用于大分子荧光偏振测定的组合物和试剂盒
公开(公告)号：US06632613B1
公开(公告)日：2003-10-14
申请号：US08483500
申请日：1995-06-07
申请人： Ai-Ping Wei , James N. Herron
发明人： Ai-Ping Wei , James N. Herron
IPC分类号： G01N3353
CPC分类号： G01N21/6428 , C07K14/59 , C12Q1/6818 , G01N21/6445 , G01N33/542 , G01N33/54373 , G01N33/582 , G01N2021/6441 , Y10S435/975
摘要： An improved oligopeptide composition for use in a fluorescent polarization immunoassay for a high molecular weight analyte is disclosed, along with a kit and a method using the composition. The composition comprises an oligopeptide selected by a screening procedure in which a plurality of different oligopeptides having respective amino acid sequences that represent sequential overlapping segments of the analyte amino acid sequence, and a fluorescent label bound thereto. In a preferred embodiment, the oligopeptide has an amino acid sequence which does not form internal disulfide bridges. Such a preferred oligopeptide will generally have no more than one cysteine residue. In a further preferred embodiment, the fluorescent label is tetramethylrhodamine or a cyanine dye. The kit may be packaged with instructions directing a user to prepare an assay solution containing the monoclonal antibody and the oligopeptide in certain respective concentrations. The composition, method and kit are preferably constructed to detect nanomolar concentrations of the analyte.
摘要翻译：公开了用于高分子量分析物的荧光偏振免疫测定法的改进的寡肽组合物以及试剂盒和使用该组合物的方法。该组合物包含通过筛选方法选择的寡肽，其中多个具有代表分析物氨基酸序列的顺序重叠片段的氨基酸序列的不同寡肽和与其结合的荧光标记。在优选的实施方案中，寡肽具有不形成内部二硫桥的氨基酸序列。这种优选的寡肽通常具有不超过一个半胱氨酸残基。在另一优选实施方案中，荧光标记是四甲基罗丹明或花青染料。试剂盒可以包装，指示用户在某些相应浓度下制备含有单克隆抗体和寡肽的测定溶液。组合物，方法和试剂盒优选构建成检测纳摩尔浓度的分析物

4. 发明授权

US5245551A Method of determining extinction coefficient of fluorescent dye and protein concentration of dye-protein conjugate 失效
标题翻译：确定荧光染料消光系数和染料 - 蛋白质结合蛋白浓度的方法
公开(公告)号：US5245551A
公开(公告)日：1993-09-14
申请号：US683145
申请日：1991-04-10
申请人： James N. Herron , Ai-Ping Wei
发明人： James N. Herron , Ai-Ping Wei
IPC分类号： G01N21/64 , G01N33/68
CPC分类号： G01N33/68 , G01N21/64 , G01N2201/129
摘要： The extinction coefficient of fluorescent dyes in donor-acceptor energy transfer processes is determined for dye-protein conjugates by forming short polypeptide fragment-dye conjugates and measuring the change in ultraviolet light absorbance of the conjugates with changing concentration. The protein concentration is determined by comparing the absorption spectrum of a conjugate of donor dye, acceptor dye, and protein with the individual components thereof using a multiple linear regression technique based on the following model:A.sub.p-d =.alpha..multidot.A.sub.p +.beta..multidot.A.sub.d +.epsilon.where, A.sub.p-d, A.sub.p, A.sub.d are the absorption spectra of the dye-protein conjugate, the protein alone, and the dye-polypeptide compound, respectively, .alpha., .beta. are the regression coefficients to be determined, and .epsilon. is the error term.
摘要翻译：通过形成短的多肽片段 - 染料缀合物并测量缀合物的紫外光吸光度随着浓度变化的变化，确定了染料 - 蛋白质缀合物中荧光染料在供体 - 受体能量转移过程中的消光系数。通过使用基于以下模型的多元线性回归技术比较供体染料，受体染料和蛋白质的缀合物与其各个组分的吸收光谱来确定蛋白质浓度：Ap-d =α×ΔP+β×Ad +ε，其中， Ap-d，Ap，Ad分别是染料 - 蛋白质缀合物，单独的蛋白质和染料 - 多肽化合物的吸收光谱，α，β是要确定的回归系数，ε是误差项。

5. 发明授权

US06482655B1 Immunoassay procedure utilizing fluorogenic tracer antigens 失效
标题翻译：使用荧光示踪剂抗原的免疫测定方法
公开(公告)号：US06482655B1
公开(公告)日：2002-11-19
申请号：US08096338
申请日：1993-07-23
申请人： Ai-Ping Wei , James N. Herron
发明人： Ai-Ping Wei , James N. Herron
IPC分类号： G01N3353
CPC分类号： G01N33/582 , C12Q1/6818 , Y10S436/80 , Y10S436/805
摘要： Disclosed are fluorescent energy transfer dyes which are capable of moving between a more stacked configuration to exhibit fluorescent quenching and a more spaced configuration to exhibit fluorescence can be conjugated to a peptide epitope for use in the detection of an unknown antibody in bulk solution. The resulting labeled peptide reagent can be used in an immunoassay procedure by placing it in bulk solution along with the unknown antibody to be detected. When the antibody binds to the peptide epitope, the pair of dyes carried by the peptide epitope will have their configuration altered from a stacked to an unstacked configuration and will exhibit a fluorescent increase in response thereto.
摘要翻译：公开了能够在更叠层的配置之间移动以展示荧光猝灭和更间隔的构型以显示荧光的荧光能量转移染料可以缀合到肽表位，以用于检测本体溶液中的未知抗体。所得标记的肽试剂可以在免疫测定程序中使用，将其与待检测的未知抗体一起放入本体溶液中。当抗体与肽表位结合时，由肽表位携带的一对染料将具有从堆叠到未堆叠构型的改变，并且对其反应将呈现荧光增加。

6. 发明授权

US5237515A Method of determining energy transfer efficiency of a donor-acceptor pair of fluorescent dyes 失效
标题翻译：确定荧光染料供体 - 受体对能量转移效率的方法
公开(公告)号：US5237515A
公开(公告)日：1993-08-17
申请号：US683146
申请日：1991-04-10
申请人： James N. Herron , Ai-Ping Wei
发明人： James N. Herron , Ai-Ping Wei
IPC分类号： G01N21/64
CPC分类号： G01N21/6428 , G01N2021/6421 , G01N2201/129
摘要： The energy transfer efficiency of a donor-acceptor pair of fluorescent dyes can be determined by first measuring the fluorescence and absorption spectra of donor-protein, acceptor-protein, mixture of donor-protein and acceptor-protein, and donor-acceptor-protein conjugates, then separating the respective spectra into their respective donor-protein complex and acceptor-protein complex components using multiple linear regression, and then determining the transfer efficiency on the basis of the quenching of the donor fluorescence based on the spectral data thus obtained.
摘要翻译：供体 - 受体对荧光染料的能量转移效率可以通过首先测量供体 - 蛋白质，受体 - 蛋白质，供体 - 蛋白质和受体 - 蛋白质的混合物和供体 - 受体 - 蛋白质缀合物的荧光和吸收光谱来确定，然后使用多元线性回归将相应的光谱分离成它们各自的供体 - 蛋白质复合物和受体 - 蛋白质复合物组分，然后基于由此获得的光谱数据基于供体荧光的猝灭确定转移效率。

7. 发明申请

US20050148620A1 Sulfonamido ether substituted imidazoquinolines 审中-公开
标题翻译：磺酰胺基醚取代的咪唑并喹啉
公开(公告)号：US20050148620A1
公开(公告)日：2005-07-07
申请号：US11069035
申请日：2005-02-28
申请人： Stephen Crooks , George Griesgraber , Philip Heppner , Bryon Merrill , Ralph Roberts , Ai-Ping Wei
发明人： Stephen Crooks , George Griesgraber , Philip Heppner , Bryon Merrill , Ralph Roberts , Ai-Ping Wei
IPC分类号： A61P31/12 , A61P37/02 , C07D471/04 , A61K31/4745 , C07D471/02
CPC分类号： C07D471/04
摘要： Imidazoquinoline and tetrahydroimidazoquinoline compounds that contain ether and sulfonamide or sulfamide functionality at the 1-position are useful as immune response modifiers. The compounds and compositions of the invention can induce the biosynthesis of various cytokines and are useful in the treatment of a variety of conditions including viral diseases and neoplastic diseases.
摘要翻译：在1位含有醚和磺酰胺或磺酰胺官能团的咪唑并喹啉和四氢咪唑并喹啉化合物可用作免疫应答调节剂。本发明的化合物和组合物可以诱导各种细胞因子的生物合成，并且可用于治疗各种病症，包括病毒性疾病和肿瘤性疾病。

8. 发明申请

US20050089946A1 Fluorogenic protease substrates based on dye-dimerization 失效
标题翻译：基于染料二聚化的荧光蛋白酶底物
公开(公告)号：US20050089946A1
公开(公告)日：2005-04-28
申请号：US10930522
申请日：2004-08-31
申请人： Ai-Ping Wei , Michael Williams
发明人： Ai-Ping Wei , Michael Williams
IPC分类号： C07K7/06 , C12Q1/04 , C12Q1/37 , C12Q1/68
CPC分类号： C07K7/06 , C12Q1/37 , C12Q2337/40 , Y02A50/451
摘要： A method of biological assay comprises the steps of providing an enzyme substrate comprising two fluorescence dye groups bound to a flexible peptide, the dye groups being of proximity sufficiently close so as to allow free energy attractions to draw the dyes together to essentially self-quench fluorescence of the dye groups, wherein self quenching of fluorescence of the dye groups is effected by dye dimerization or stacking, and enzymatically cleaving the peptide to release the fluorescence dye groups from dye dimerization or stacking, thereby producing an increase in fluorescence intensity. A protease substrate for use in the method of the invention is also disclosed. This invention finds use in detection and identification of microorganisms, sterilization assurance, pharmaceutical discovery, enzyme assays, immunoassays, and other biological assays.
摘要翻译：一种生物测定方法包括以下步骤：提供包含与柔性肽结合的两个荧光染料基团的酶底物，所述染料组的接近度足够接近，以便使自由能吸引力将染料一起吸收到基本上自熄灭荧光的染料基团，其中染料基团的荧光自发淬灭通过染料二聚化或堆叠进行，并且酶切割肽以使荧光染料基团从染料二聚化或堆叠释放，从而产生荧光强度的增加。还公开了用于本发明方法的蛋白酶底物。本发明用于微生物的检测和鉴定，灭菌保证，药物发现，酶测定，免疫测定和其他生物测定。

9. 发明授权

US08325345B2 Methods and devices for monitoring of frying oil quality 有权
标题翻译：油炸油质量监测方法和装置
公开(公告)号：US08325345B2
公开(公告)日：2012-12-04
申请号：US12920520
申请日：2009-03-02
申请人： Abolghassem B. Mahmoodi , Milind Balwant Sabade , Ai-Ping Wei
发明人： Abolghassem B. Mahmoodi , Milind Balwant Sabade , Ai-Ping Wei
IPC分类号： G01N21/55 , G01N33/03 , G01N33/26
CPC分类号： G01N33/03 , G01N21/78 , G01N31/22
摘要： Herein are disclosed methods and devices for evaluating the quality of oils (e.g., cooking oil or flying oil). The methods can provide an indication of the free fatty acid content of the oil. The methods use an optical interrogation device to provide an indication of free fatty acid based on quantitative measurements of optical reflectance from test zones on a sampling substrate.
摘要翻译：这里公开了用于评估油（例如烹饪油或飞油）的质量的方法和装置。该方法可以提供油的游离脂肪酸含量的指示。该方法使用光学询问装置来提供游离脂肪酸的指示，其基于在取样基底上的测试区域的光学反射率的定量测量。

10. 发明申请

US20110183360A1 ANTIBODIES TO CLOSTRIDIUM DIFFICILE SPORES AND USES THEREOF 失效
标题翻译：抗精神分裂症的运动及其用途
公开(公告)号：US20110183360A1
公开(公告)日：2011-07-28
申请号：US12919254
申请日：2009-02-25
申请人： Raj Rajagopal , Ai-Ping Wei
发明人： Raj Rajagopal , Ai-Ping Wei
IPC分类号： C12N5/12 , G01N33/569 , C07K16/12 , C07K16/40 , C12N5/10 , C07H21/00 , C12N15/63 , C12N1/00
CPC分类号： G01N33/56911 , C07K16/1282 , C07K16/40 , G01N2333/33 , G01N2469/10 , G01N2800/067
摘要： The present invention provides antibodies that bind to the endospore of the bacterium Clostridium difficile, methods of making such antibodies, and methods of using such antibodies, including methods of detecting C. difficile endospores.
摘要翻译：本发明提供了结合细菌艰难梭菌的内生孢子的抗体，制备这种抗体的方法，以及使用这些抗体的方法，包括检测艰难梭菌内切孢子的方法。

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