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1. 发明申请

US20110195442A1 STERILITY INDICATING BIOLOGICAL COMPOSITIONS, ARTICLES AND METHODS 审中-公开
标题翻译：表明生物组成的无效性，文章和方法
公开(公告)号：US20110195442A1
公开(公告)日：2011-08-11
申请号：US13123824
申请日：2009-10-15
申请人： Sailaja Chandrapati , Heather M. Webb , Ai-Ping Wei
发明人： Sailaja Chandrapati , Heather M. Webb , Ai-Ping Wei
IPC分类号： C12Q1/37
CPC分类号： C12Q1/37 , A61L2/28 , C12Q1/22
摘要： A sterility indicating composition comprising a plurality of sterilization process resistant spores which contain an active protease during germination and initial outgrowth of the spores; and a germination medium comprising at least one labeled protease substrate and at least one nutrient for germination of the spores; wherein the medium is essentially free of a) any active protease other than the active protease contained by the plurality of spores and b) any protease substrate other than the at least one labeled protease substrate, other than any protease substrate originating from the plurality of spores, and other than any protease substrate which does not compete with the labeled protease substrate for the active protease; and wherein the at least one labeled protease substrate comprises a peptide which can be cleaved by the active protease and which is labeled with one or more dye groups, at least one of which undergoes a detectable change when the peptide is cleaved by the active protease, and wherein the labeled protease substrate is stable at least at a temperature for incubating the spores, a sterilization process indicator comprising the composition, and a method of determining the effectiveness of a sterilization process using the composition and indicator are disclosed.
摘要翻译：一种不育指示组合物，其包含多个灭菌过程抗性孢子，其在萌发期间含有活性蛋白酶和孢子的初始产生; 以及包含至少一种标记的蛋白酶底物和至少一种用于孢子发芽的营养物的发芽培养基; 其中所述培养基基本上不含a）除所述多个孢子所含的活性蛋白酶以外的任何活性蛋白酶，和b）除所述至少一个标记的蛋白酶底物之外的任何蛋白酶底物，而不是源于所述多个孢子的任何蛋白酶底物，而不是与活性蛋白酶的标记的蛋白酶底物竞争的任何蛋白酶底物以外的其它蛋白酶底物; 并且其中所述至少一个标记的蛋白酶底物包含可被活性蛋白酶切割并且被一个或多个染料基团标记的肽，当肽被活性蛋白酶切割时，其至少一种经历可检测的变化，并且其中标记的蛋白酶底物至少在用于培养孢子的温度下是稳定的，包括该组合物的灭菌过程指示剂以及使用该组合物和指示剂确定灭菌过程的有效性的方法。

2. 发明申请

US20080306252A1 SULFONAMIDO ETHER SUBSTITUTED IMIDAZOQUINOLINES 审中-公开
标题翻译：磺酰胺取代的咪唑啉醇
公开(公告)号：US20080306252A1
公开(公告)日：2008-12-11
申请号：US12131023
申请日：2008-05-30
申请人： Stephen L. Crooks , George W. Griesgraber , Philip D. Heppner , Bryon A. Merrill , Ralph R. Roberts , Ai-Ping Wei
发明人： Stephen L. Crooks , George W. Griesgraber , Philip D. Heppner , Bryon A. Merrill , Ralph R. Roberts , Ai-Ping Wei
IPC分类号： C09B29/36 , C07D471/04
CPC分类号： C07D471/04
摘要： Imidazoquinoline and tetrahydroimidazoquinoline compounds that contain ether and sulfonamide or sulfamide functionality at the 1-position are useful as immune response modifiers. The compounds and compositions of the invention can induce the biosynthesis of various cytokines and are useful in the treatment of a variety of conditions including viral diseases and neoplastic diseases.
摘要翻译：在1位含有醚和磺酰胺或磺酰胺官能团的咪唑并喹啉和四氢咪唑并喹啉化合物可用作免疫应答调节剂。本发明的化合物和组合物可以诱导各种细胞因子的生物合成，并且可用于治疗各种病症，包括病毒性疾病和肿瘤性疾病。

3. 发明授权

US07256012B2 Fluorogenic protease substrates based on dye-dimerization 失效
标题翻译：基于染料二聚化的荧光蛋白酶底物
公开(公告)号：US07256012B2
公开(公告)日：2007-08-14
申请号：US10930522
申请日：2004-08-31
申请人： Ai-Ping Wei , Michael George Williams
发明人： Ai-Ping Wei , Michael George Williams
IPC分类号： C12Q1/37 , C12Q1/04 , C07K7/06 , G01N2/64
CPC分类号： C07K7/06 , C12Q1/37 , C12Q2337/40 , Y02A50/451
摘要： A method of biological assay comprises the steps of providing an enzyme substrate comprising two fluorescence dye groups bound to a flexible peptide, the dye groups being of proximity sufficiently close so as to allow free energy attractions to draw the dyes together to essentially self-quench fluorescence of the dye groups, wherein self quenching of fluorescence of the dye groups is effected by dye dimerization or stacking, and enzymatically cleaving the peptide to release the fluorescence dye groups from dye dimerization or stacking, thereby producing an increase in fluorescence intensity. A protease substrate for use in the method of the invention is also disclosed. This invention finds use in detection and identification of microorganisms, sterilization assurance, pharmaceutical discovery, enzyme assays, immunoassays, and other biological assays.
摘要翻译：一种生物测定方法包括以下步骤：提供包含与柔性肽结合的两个荧光染料基团的酶底物，所述染料组的接近度足够接近，以便使自由能吸引力将染料一起吸收到基本上自熄灭荧光的染料基团，其中染料基团的荧光自发淬灭通过染料二聚化或堆叠进行，并且酶切割肽以使荧光染料基团从染料二聚化或堆叠释放，从而产生荧光强度的增加。还公开了用于本发明方法的蛋白酶底物。本发明用于微生物的检测和鉴定，灭菌保证，药物发现，酶测定，免疫测定和其他生物测定。

4. 发明授权

US07132429B2 Sulfonamido ether substituted imidazoquinolines 失效
公开(公告)号：US07132429B2
公开(公告)日：2006-11-07
申请号：US11069033
申请日：2005-02-28
申请人： George W. Griesgraber , Bryon A. Merrill , Ralph R. Roberts , Ai-Ping Wei
发明人： George W. Griesgraber , Bryon A. Merrill , Ralph R. Roberts , Ai-Ping Wei
IPC分类号： A01N43/42 , A01N31/44 , C07D47/00 , C07D491/00 , C07D498/00
CPC分类号： C07D471/04 , A61K31/4745 , Y02A50/409
摘要： Imidazoquinoline and tetrahydroimidazoquinoline compounds that contain ether and sulfonamide or sulfamide functionality at the 1-position are useful as immune response modifiers. The compounds and compositions of the invention can induce the biosynthesis of various cytokines and are useful in the treatment of a variety of conditions including viral diseases and neoplastic diseases.

5. 发明申请

US20050148619A1 Sulfonamido ether substituted imidazoquinolines 失效
公开(公告)号：US20050148619A1
公开(公告)日：2005-07-07
申请号：US11069033
申请日：2005-02-28
申请人： Stephen Crooks , George Griesgraber , Philip Heppner , Bryon Merrill , Ralph Roberts , Ai-Ping Wei
发明人： Stephen Crooks , George Griesgraber , Philip Heppner , Bryon Merrill , Ralph Roberts , Ai-Ping Wei
IPC分类号： C12P21/02 , A61K31/437 , A61K31/4745 , A61K31/506 , A61K31/5377 , A61K31/655 , A61P1/16 , A61P7/00 , A61P11/06 , A61P15/00 , A61P17/00 , A61P17/02 , A61P17/04 , A61P17/14 , A61P25/00 , A61P25/28 , A61P27/02 , A61P29/00 , A61P31/00 , A61P31/04 , A61P31/06 , A61P31/08 , A61P31/10 , A61P31/12 , A61P31/14 , A61P31/16 , A61P31/18 , A61P31/20 , A61P31/22 , A61P33/00 , A61P33/02 , A61P33/08 , A61P35/00 , A61P35/02 , A61P37/00 , A61P37/02 , A61P37/04 , A61P37/06 , A61P37/08 , A61P43/00 , C07D471/02 , C07D471/04
CPC分类号： C07D471/04 , A61K31/4745 , Y02A50/409
摘要： Imidazoquinoline and tetrahydroimidazoquinoline compounds that contain ether and sulfonamide or sulfamide functionality at the 1-position are useful as immune response modifiers. The compounds and compositions of the invention can induce the biosynthesis of various cytokines and are useful in the treatment of a variety of conditions including viral diseases and neoplastic diseases.

6. 发明授权

US06787329B1 Fluorogenic protease substrates based on dye-dimerization 失效
标题翻译：基于染料二聚化的荧光蛋白酶底物
公开(公告)号：US06787329B1
公开(公告)日：2004-09-07
申请号：US09448633
申请日：1999-11-24
申请人： Ai-Ping Wei , Michael George Williams
发明人： Ai-Ping Wei , Michael George Williams
IPC分类号： C12Q137
CPC分类号： C07K7/06 , C12Q1/37 , C12Q2337/40 , Y02A50/451
摘要： A method of biological assay comprises the steps of providing an enzyme substrate comprising two fluorescence dye groups bound to a flexible peptide, the dye groups being of proximity sufficiently close so as to allow free energy attractions to draw the dyes together to essentially self-quench fluorescence of the dye groups, wherein self quenching of fluorescence of the dye groups is effected by dye dimerization or stacking, and enzymatically cleaving the peptide to release the fluorescence dye groups from dye dimerization or stacking, thereby producing an increase in fluorescence intensity. A protease substrate for use in the method of the invention is also disclosed. This invention finds use in detection and identification of microorganisms, sterilization assurance, pharmaceutical discovery, enzyme assays, immunoassays, and other biological assays.
摘要翻译：一种生物测定方法包括以下步骤：提供包含与柔性肽结合的两个荧光染料基团的酶底物，所述染料组的接近度足够接近，以便使自由能吸引力将染料一起吸收到基本上自熄灭荧光的染料基团，其中染料基团的荧光自发淬灭通过染料二聚化或堆叠进行，并且酶切割肽以使荧光染料基团从染料二聚化或堆叠释放，从而产生荧光强度的增加。还公开了用于本发明方法的蛋白酶底物。本发明用于微生物的检测和鉴定，灭菌保证，药物发现，酶测定，免疫测定和其他生物测定。

7. 发明授权

US06683088B2 Sulfonamido ether substituted imidazoquinolines 失效
公开(公告)号：US06683088B2
公开(公告)日：2004-01-27
申请号：US10012599
申请日：2001-12-06
申请人： Stephen L. Crooks , George W. Griesgraber , Philip D. Heppner , Bryon A. Merrill , Ralph R. Roberts , Ai-Ping Wei
发明人： Stephen L. Crooks , George W. Griesgraber , Philip D. Heppner , Bryon A. Merrill , Ralph R. Roberts , Ai-Ping Wei
IPC分类号： A61K314745
CPC分类号： C07D471/04 , A61K31/4745 , Y02A50/409
摘要： Imidazoquinoline and tetrahydroimidazoquinoline compounds that contain ether and sulfonamide or sulfamide functionality at the 1-position are useful as immune response modifiers. The compounds and compositions of the invention can induce the biosynthesis of various cytokines and are useful in the treatment of a variety of conditions including viral diseases and neoplastic diseases.

8. 发明授权

US06482655B1 Immunoassay procedure utilizing fluorogenic tracer antigens 失效
标题翻译：使用荧光示踪剂抗原的免疫测定方法
公开(公告)号：US06482655B1
公开(公告)日：2002-11-19
申请号：US08096338
申请日：1993-07-23
申请人： Ai-Ping Wei , James N. Herron
发明人： Ai-Ping Wei , James N. Herron
IPC分类号： G01N3353
CPC分类号： G01N33/582 , C12Q1/6818 , Y10S436/80 , Y10S436/805
摘要： Disclosed are fluorescent energy transfer dyes which are capable of moving between a more stacked configuration to exhibit fluorescent quenching and a more spaced configuration to exhibit fluorescence can be conjugated to a peptide epitope for use in the detection of an unknown antibody in bulk solution. The resulting labeled peptide reagent can be used in an immunoassay procedure by placing it in bulk solution along with the unknown antibody to be detected. When the antibody binds to the peptide epitope, the pair of dyes carried by the peptide epitope will have their configuration altered from a stacked to an unstacked configuration and will exhibit a fluorescent increase in response thereto.
摘要翻译：公开了能够在更叠层的配置之间移动以展示荧光猝灭和更间隔的构型以显示荧光的荧光能量转移染料可以缀合到肽表位，以用于检测本体溶液中的未知抗体。所得标记的肽试剂可以在免疫测定程序中使用，将其与待检测的未知抗体一起放入本体溶液中。当抗体与肽表位结合时，由肽表位携带的一对染料将具有从堆叠到未堆叠构型的改变，并且对其反应将呈现荧光增加。

9. 发明授权

US5237515A Method of determining energy transfer efficiency of a donor-acceptor pair of fluorescent dyes 失效
标题翻译：确定荧光染料供体 - 受体对能量转移效率的方法
公开(公告)号：US5237515A
公开(公告)日：1993-08-17
申请号：US683146
申请日：1991-04-10
申请人： James N. Herron , Ai-Ping Wei
发明人： James N. Herron , Ai-Ping Wei
IPC分类号： G01N21/64
CPC分类号： G01N21/6428 , G01N2021/6421 , G01N2201/129
摘要： The energy transfer efficiency of a donor-acceptor pair of fluorescent dyes can be determined by first measuring the fluorescence and absorption spectra of donor-protein, acceptor-protein, mixture of donor-protein and acceptor-protein, and donor-acceptor-protein conjugates, then separating the respective spectra into their respective donor-protein complex and acceptor-protein complex components using multiple linear regression, and then determining the transfer efficiency on the basis of the quenching of the donor fluorescence based on the spectral data thus obtained.
摘要翻译：供体 - 受体对荧光染料的能量转移效率可以通过首先测量供体 - 蛋白质，受体 - 蛋白质，供体 - 蛋白质和受体 - 蛋白质的混合物和供体 - 受体 - 蛋白质缀合物的荧光和吸收光谱来确定，然后使用多元线性回归将相应的光谱分离成它们各自的供体 - 蛋白质复合物和受体 - 蛋白质复合物组分，然后基于由此获得的光谱数据基于供体荧光的猝灭确定转移效率。

10. 发明授权

US08697374B2 Antibodies to Clostridium difficile spores and uses thereof 失效
标题翻译：艰难梭菌孢子的抗体及其用途
公开(公告)号：US08697374B2
公开(公告)日：2014-04-15
申请号：US12919254
申请日：2009-02-25
申请人： Raj Rajagopal , Ai-Ping Wei
发明人： Raj Rajagopal , Ai-Ping Wei
IPC分类号： G01N33/554 , G01N33/569
CPC分类号： G01N33/56911 , C07K16/1282 , C07K16/40 , G01N2333/33 , G01N2469/10 , G01N2800/067
摘要： The present invention provides antibodies that bind to the endospore of the bacterium Clostridium difficile, methods of making such antibodies, and methods of using such antibodies, including methods of detecting C. difficile endospores.
摘要翻译：本发明提供了结合细菌艰难梭菌的内生孢子的抗体，制备这种抗体的方法，以及使用这些抗体的方法，包括检测艰难梭菌内切孢子的方法。

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