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1. 发明授权

US5739409A Endogenously sweetened transgenic plant products 失效
标题翻译：内源性甜化转基因植物产品
公开(公告)号：US5739409A
公开(公告)日：1998-04-14
申请号：US483415
申请日：1995-06-07
申请人： Robert Fischer , Sung-Hou Kim , Joong Myung Cho , Lola Penarrubia , James Giovannoni , Rosalind Kim
发明人： Robert Fischer , Sung-Hou Kim , Joong Myung Cho , Lola Penarrubia , James Giovannoni , Rosalind Kim
IPC分类号： A01H4/00 , A23L27/00 , A23L27/21 , C07H21/04 , C07K14/43 , C12N5/06 , C12N5/14 , C12N15/03 , C12N15/82 , C12P21/06
CPC分类号： C07K14/43
摘要： Edible fruit, seed and vegetables of transgenic plants modified to produce a sweetening protein such as monellin or thaumatin are useful in preparing food compositions which have enhanced sweetness improved flavor. Expression systems for the genes encoding sweetening proteins compatible with plant systems and designed to enhance the production of these proteins in the edible portions of plants, and methods for producing sweetened fruit, seeds and vegetables are described.
摘要翻译：经改性以产生甜味蛋白如芒奈林或马蹄莲的转基因植物的食用水果，种子和蔬菜可用于制备具有增强的甜度改善风味的食品组合物。描述了编码与植物系统相容的甜味蛋白的基因的表达系统，并被设计用于增强植物可食用部分中这些蛋白质的产生，以及用于生产甜果，种子和蔬菜的方法。

2. 发明授权

US5168064A Endo-1,4-.beta.-glucanase gene and its use in plants 失效
标题翻译： Endo-1,4β-葡聚糖酶基因及其在植物中的应用
公开(公告)号：US5168064A
公开(公告)日：1992-12-01
申请号：US511417
申请日：1990-04-20
申请人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
发明人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
IPC分类号： A01H5/00 , A01H1/02 , C12N5/10 , C12N9/24 , C12N9/42 , C12N15/09 , C12N15/82 , C12N15/84
CPC分类号： C12N9/2437 , A01H1/02 , C12N15/8249 , C12Y302/01004
摘要： The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense DNA constructions.
摘要翻译：本发明提供了一种通过使用反义DNA构建抑制内-4,4-β-葡聚糖酶活性来减少果实软化和细胞壁多糖降解的方法。

3. 发明授权

US07124980B2 Wire spool guide assembly 失效
标题翻译：线卷轴导向组件
公开(公告)号：US07124980B2
公开(公告)日：2006-10-24
申请号：US10921236
申请日：2004-08-19
申请人： James Giovannoni
发明人： James Giovannoni
IPC分类号： B65H75/20 , B65H49/18
CPC分类号： B65H49/321 , B65H57/06
摘要： The wire spool guide assembly is a conduit through which wire is dispensed in a controlled and orderly manner. The assembly has at least one wire spool guide, a handle bar and a spindle. The spindle may be a single spindle or part of a wire spool cart. The spindle is used to hold wire spools and the handle bar. The handle bar serves to hold the guide. The guide includes an eyebolt rotatably mounted to a support frame via a ball bearing. The eyebolt guides wire as it is dispensed from the spool acquiescing to the direction of the user's pull. Friction, created as wire is pulled through the eyebolt, slows the speed at which the spool rotates on the spindle. The frame of the guide, however, is fixed on the handle bar by a fastener disposed at a bottom end of the guide.
摘要翻译：线卷轴导向组件是以受控和有序的方式分配线的导管。该组件具有至少一个线卷轴引导件，手柄杆和主轴。主轴可以是单主轴或电线卷筒车的一部分。主轴用于保持线轴和手柄。手柄用于固定导板。引导件包括通过滚珠轴承可旋转地安装到支撑框架上的吊环螺栓。吊环引导线从线轴分配，默认为用户拉的方向。作为电线产生的摩擦被拉过眼螺栓，减慢了阀芯在主轴上旋转的速度。然而，引导件的框架通过布置在引导件的底端处的紧固件固定在把手上。

4. 发明授权

US06787687B1 Rin gene compositions and methods for use thereof 有权
标题翻译： Rin基因组合物及其使用方法
公开(公告)号：US06787687B1
公开(公告)日：2004-09-07
申请号：US09614981
申请日：2000-07-12
申请人： James Giovannoni , Steven Tanksley , Veeraragavan Padmanabhan , Diane Ruezinsky , Julie Vrebalov , Ruth White
发明人： James Giovannoni , Steven Tanksley , Veeraragavan Padmanabhan , Diane Ruezinsky , Julie Vrebalov , Ruth White
IPC分类号： C12N1529
CPC分类号： C07K14/415 , C12N15/8216 , C12N15/8223 , C12N15/8235 , C12N15/8249 , C12N15/825 , C12N15/8291
摘要： The current invention provides nucleic acid sequences encoding the RIN and MC genes. Compositions comprising these sequences are described, as are plants transformed with such compositions. Further provided are methods for the expression of the RIN and MC genes. The methods of the invention include the direct creation of transgenic plants with the RIN and MC genes by genetic transformnation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.
摘要翻译：本发明提供编码RIN和MC基因的核酸序列。描述了包含这些序列的组合物，以及用这种组合物转化的植物也是如此。还提供了表达RIN和MC基因的方法。本发明的方法包括通过遗传转化直接产生具有RIN和MC基因的转基因植物，以及通过植物育种方法。本发明的序列代表了用于产生转基因植物的有价值的新工具，优选具有一个或多个添加的有益特征。

5. 发明申请

US20050076408A1 RIN gene compositions and methods for use thereof 审中-公开
标题翻译： RIN基因组合物及其使用方法
公开(公告)号：US20050076408A1
公开(公告)日：2005-04-07
申请号：US10934086
申请日：2004-09-03
申请人： James Giovannoni , Steven Tanksley , Veeraragavan Padmanabhan , Diane Ruezinsky , Julie Vrebalov , Ruth White
发明人： James Giovannoni , Steven Tanksley , Veeraragavan Padmanabhan , Diane Ruezinsky , Julie Vrebalov , Ruth White
IPC分类号： C07K14/415 , C12N15/29 , C12N15/82 , C12N15/84 , A01H1/00 , C07H21/04
CPC分类号： C07K14/415 , C12N15/8216 , C12N15/8223 , C12N15/8235 , C12N15/8249 , C12N15/825 , C12N15/8291
摘要： The current invention provides nucleic acid sequences encoding the RIN and MC genes. Compositions comprising these sequences are described, as are plants transformed with such compositions. Further provided are methods for the expression of the RIN and MC genes. The methods of the invention include the direct creation of transgenic plants with the RIN and MC genes by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.
摘要翻译：本发明提供编码RIN和MC基因的核酸序列。描述了包含这些序列的组合物，以及用这种组合物转化的植物也是如此。还提供了表达RIN和MC基因的方法。本发明的方法包括通过遗传转化直接产生具有RIN和MC基因的转基因植物，以及通过植物育种方法。本发明的序列代表了用于产生转基因植物的有价值的新工具，优选具有一个或多个添加的有益特征。

6. 发明授权

US06762347B1 NOR gene compositions and methods for use thereof 有权
标题翻译： NOR基因组合物及其使用方法
公开(公告)号：US06762347B1
公开(公告)日：2004-07-13
申请号：US09614408
申请日：2000-07-12
申请人： James Giovannoni , Steven Tanksley , Julia Vrebalov , Frederick Noensie
发明人： James Giovannoni , Steven Tanksley , Julia Vrebalov , Frederick Noensie
IPC分类号： C12N1582
CPC分类号： C12N15/825 , C07K14/415 , C12N15/8249 , C12N15/8291
摘要： The current invention provides nucleic acid sequences encoding the NOR gene. Compositions comprising this sequence are described, as are plants transformed with such compositions. Further provided are methods for the expression of the NOR gene. The methods of the invention include the direct creation of transgenic plants with the NOR gene by genetic transformation, as well as by plant breeding methods. The sequences of the invention represent a valuable new tool for the creation of transgenic plants, preferably having one or more added beneficial characteristics.
摘要翻译：本发明提供了编码NOR基因的核酸序列。描述了包含该序列的组合物，以及用这种组合物转化的植物。还提供了用于表达NOR基因的方法。本发明的方法包括通过遗传转化直接产生具有NOR基因的转基因植物，以及通过植物育种方法。本发明的序列代表了用于产生转基因植物的有价值的新工具，优选具有一个或多个添加的有益特征。

7. 发明授权

US5585545A Endo-1,4-.beta.-glucanase genes and their use in plants 失效
标题翻译： Endo-1,4β-葡聚糖酶基因及其在植物中的应用
公开(公告)号：US5585545A
公开(公告)日：1996-12-17
申请号：US271883
申请日：1994-07-07
申请人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
发明人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
IPC分类号： A01H5/00 , A01H1/02 , C12N5/10 , C12N9/24 , C12N9/42 , C12N15/09 , C12N15/82 , C12N15/84 , C12N15/29 , C12N15/56
CPC分类号： A01H1/02 , C12N15/8249 , C12N9/2437 , C12N9/244 , C12Y302/01004 , C12Y302/01006
摘要： The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense nucleic acid constructions.
摘要翻译：本发明提供了一种通过使用反义核酸构建抑制内-4,4-β-葡聚糖酶活性来减少果实软化和细胞壁多糖降解的方法。

8. 发明授权

US5328999A Endo-1,4-.beta.-glucanase genes and their use in plants 失效
标题翻译： Endo-1,4β-葡聚糖酶基因及其在植物中的应用
公开(公告)号：US5328999A
公开(公告)日：1994-07-12
申请号：US687466
申请日：1991-04-18
申请人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
发明人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
IPC分类号： A01H5/00 , A01H1/02 , C12N5/10 , C12N9/24 , C12N9/42 , C12N15/09 , C12N15/82 , C12N15/84 , C07H15/12 , C12N5/00 , C12N15/00
CPC分类号： C12N9/2437 , A01H1/02 , C12N15/8249 , C12Y302/01004
摘要： The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense DNA constructions.
摘要翻译：本发明提供了一种通过使用反义DNA构建抑制内-4,4-β-葡聚糖酶活性来减少果实软化和细胞壁多糖降解的方法。

9. 发明授权

US5234834A Constructs for expression of monellin in plant cells 失效
标题翻译：在植物细胞中表达MONELLIN的构建体
公开(公告)号：US5234834A
公开(公告)日：1993-08-10
申请号：US557222
申请日：1990-07-24
申请人： Robert Fischer , Sung-Hou Kim , Joong M. Cho , Lola Penarrubia , James Giovannoni , Rosalind Kim
发明人： Robert Fischer , Sung-Hou Kim , Joong M. Cho , Lola Penarrubia , James Giovannoni , Rosalind Kim
IPC分类号： C12N15/09 , A01H1/00 , A23L1/00 , A23L1/236 , C07K1/04 , C07K1/22 , C07K14/00 , C07K14/415 , C07K14/43 , C07K19/00 , C12N1/21 , C12N15/29 , C12N15/82 , C12P21/02 , C12R1/19
CPC分类号： C12N15/8242 , C07K14/43
摘要： Edible fruit, seed and vegetables of transgenic plants modified to produce a sweetening protein such as monellin or thaumatin are useful in preparing food compositions which have enhanced sweetness improved flavor. Expression systems for the genes encoding sweetening proteins compatible with plant systems and designed to enhance the production of these proteins in the edible portions of plants, and methods for producing sweetened fruit, seeds and vegetables are described.
摘要翻译：经改性以产生甜味蛋白如芒奈林或马蹄莲的转基因植物的食用水果，种子和蔬菜可用于制备具有增强的甜度改善风味的食品组合物。描述了编码与植物系统相容的甜味蛋白的基因的表达系统，并被设计用于增强植物可食用部分中这些蛋白质的产生，以及用于生产甜果，种子和蔬菜的方法。

10. 发明授权

US07947867B1 Ripening inhibition in the tomato Green-ripe mutant results from ectopic expression of a novel protein which disrupts ethylene signal transduction 有权
标题翻译：番茄中的成熟抑制绿色成熟突变体来自破坏乙烯信号转导的新蛋白质的异位表达
公开(公告)号：US07947867B1
公开(公告)日：2011-05-24
申请号：US11442028
申请日：2006-05-26
申请人： James Giovannoni , Cornelius S. Barry
发明人： James Giovannoni , Cornelius S. Barry
IPC分类号： C12N15/29 , C12N15/82 , C12N5/04 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8249
摘要： To achieve full development of the ripe phenotype, climacteric fruits, such as tomato, apple and banana, require synthesis, perception and signal transduction of the plant hormone ethylene. The non-ripening phenotype of the dominant Green-ripe (Gr) and Never-ripe 2 (Nr-2) mutants of tomato is the result of reduced ethylene responsiveness in fruit tissues. In addition a subset of ethylene responses associated with floral senescence, abscission and root elongation are also impacted in mutant plants but to a lesser extent. Using positional cloning we have identified an identical 334 by deletion in a gene of unknown biochemical function residing at the Gr/Nr-2 locus. Consistent with a dominant gain of function mutation, this deletion causes ectopic expression of GR/NR-2, which in turn leads to ripening inhibition. A CaMV35:GR transgene recreates the Gr/Nr-2 mutant phenotype but does not lead to a global reduction in ethylene responsiveness suggesting tissue-specific modulation of ethylene responses in tomato. GR/NR-2 encodes a novel evolutionary conserved membrane localized protein of unknown biochemical function that has not previously been associated with ethylene signaling. Because GR/NR-2 has no sequence homology with the previously described Nr (Never-ripe) ethylene receptor of tomato we now refer to this gene only as GR. Identification of GR expands the current repertoire of ethylene signaling components in plants and provides a tool for further elucidation of ethylene response mechanisms and for controlling ethylene signal specificity in crop plants.
摘要翻译：为了实现成熟表型的充分发展，更年期果实如番茄，苹果和香蕉需要植物激素乙烯的合成，感知和信号转导。番茄优势绿熟（Gr）和未成熟2（Nr-2）突变体的非成熟表型是果实组织中乙烯反应降低的结果。此外，与花衰老，脱落和根伸长相关的乙烯应答亚组也在突变植物中受到影响，但程度较小。使用位置克隆，我们通过在位于Gr / Nr-2位点处的未知生物化学功能的基因中缺失鉴定了相同的334。与功能突变的主要增益一致，该缺失导致GR / NR-2的异位表达，其又导致成熟抑制。 CaMV35：GR转基因重现Gr / Nr-2突变体表型，但不会导致乙烯反应性的全面降低，表明番茄中乙烯应答的组织特异性调节。 GR / NR-2编码未知的生物化学功能的新进化保守膜定位蛋白，以前没有与乙烯信号传导有关。因为GR / NR-2与以前描述的Nr（未成熟）番茄的乙烯受体没有序列同源性，所以我们现在将该基因仅称为GR。 GR的鉴定扩展了植物中乙烯信号传导成分的当前目录，为进一步阐明乙烯反应机制和控制作物中乙烯信号特异性提供了一个工具。

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