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1. 发明申请

US20080109918A1 Materials and methods for tissue-specific targeting of ethylene insensitivity in transgenic plants 审中-公开
标题翻译：在转基因植物中组织特异性靶向乙烯不敏感的材料和方法
公开(公告)号：US20080109918A1
公开(公告)日：2008-05-08
申请号：US11823839
申请日：2007-06-28
申请人： Harry Klee , Coralie Lashbrook , Lori Shrode
发明人： Harry Klee , Coralie Lashbrook , Lori Shrode
IPC分类号： A01H5/00 , C07H21/04 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8266 , C07K14/415
摘要： The subject invention concerns materials and methods for controlling agricultural traits in plants that are mediated by the plant hormone ethylene. One aspect of the invention concerns a polynucleotide that comprises a sequence encoding a mutant ethylene receptor that is operably linked to a regulatory sequence that drives expression of the mutant receptor in a tissue-specific manner. In an exemplified embodiment, the mutant receptor sequence is an etr1-1 sequence, or a functional fragment or variant thereof, and the regulatory sequence is a promoter sequence from a cotton chitinase gene that can promote expression of the mutant ethylene receptor in abscission zone tissue of a plant. The subject invention also concerns plants and plant tissue transformed with the polynucleotide of the subject invention. Plants expressing the polynucleotide of the subject invention do not drop their flowers in response to exposure to ethylene.
摘要翻译：本发明涉及用于控制由植物激素乙烯介导的植物中的农业性状的材料和方法。本发明的一个方面涉及一种多核苷酸，其包含编码突变体乙烯受体的序列，其与以驱动组织特异性方式驱动突变受体表达的调节序列可操作地连接。在一个示例性实施方案中，突变体受体序列是etr1-1序列或其功能片段或变体，并且调节序列是来自棉花几丁质酶基因的启动子序列，其可促进突变乙烯受体在脱落区组织中的表达的植物。本发明还涉及用本发明的多核苷酸转化的植物和植物组织。表达本发明多核苷酸的植物不会因暴露于乙烯而降花。

2. 发明授权

US5554743A Endo-1,4-.beta.-glucanase genes and their use in plants 失效
公开(公告)号：US5554743A
公开(公告)日：1996-09-10
申请号：US434702
申请日：1995-05-04
申请人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
发明人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
IPC分类号： A01H5/00 , A01H1/02 , C12N5/10 , C12N9/24 , C12N9/42 , C12N15/09 , C12N15/82 , C12N15/84 , C12N15/29 , C12N15/55
CPC分类号： A01H1/02 , C12N15/8249 , C12N9/2437 , C12N9/244 , C12Y302/01004 , C12Y302/01006
摘要： The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense nucleic acid constructions.

3. 发明授权

US5168064A Endo-1,4-.beta.-glucanase gene and its use in plants 失效
标题翻译： Endo-1,4β-葡聚糖酶基因及其在植物中的应用
公开(公告)号：US5168064A
公开(公告)日：1992-12-01
申请号：US511417
申请日：1990-04-20
申请人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
发明人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
IPC分类号： A01H5/00 , A01H1/02 , C12N5/10 , C12N9/24 , C12N9/42 , C12N15/09 , C12N15/82 , C12N15/84
CPC分类号： C12N9/2437 , A01H1/02 , C12N15/8249 , C12Y302/01004
摘要： The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense DNA constructions.
摘要翻译：本发明提供了一种通过使用反义DNA构建抑制内-4,4-β-葡聚糖酶活性来减少果实软化和细胞壁多糖降解的方法。

4. 发明授权

US5585545A Endo-1,4-.beta.-glucanase genes and their use in plants 失效
标题翻译： Endo-1,4β-葡聚糖酶基因及其在植物中的应用
公开(公告)号：US5585545A
公开(公告)日：1996-12-17
申请号：US271883
申请日：1994-07-07
申请人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
发明人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
IPC分类号： A01H5/00 , A01H1/02 , C12N5/10 , C12N9/24 , C12N9/42 , C12N15/09 , C12N15/82 , C12N15/84 , C12N15/29 , C12N15/56
CPC分类号： A01H1/02 , C12N15/8249 , C12N9/2437 , C12N9/244 , C12Y302/01004 , C12Y302/01006
摘要： The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense nucleic acid constructions.
摘要翻译：本发明提供了一种通过使用反义核酸构建抑制内-4,4-β-葡聚糖酶活性来减少果实软化和细胞壁多糖降解的方法。

5. 发明授权

US5328999A Endo-1,4-.beta.-glucanase genes and their use in plants 失效
标题翻译： Endo-1,4β-葡聚糖酶基因及其在植物中的应用
公开(公告)号：US5328999A
公开(公告)日：1994-07-12
申请号：US687466
申请日：1991-04-18
申请人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
发明人： Alan B. Bennett , Robert L. Fischer , Coralie Lashbrook , James Giovannoni
IPC分类号： A01H5/00 , A01H1/02 , C12N5/10 , C12N9/24 , C12N9/42 , C12N15/09 , C12N15/82 , C12N15/84 , C07H15/12 , C12N5/00 , C12N15/00
CPC分类号： C12N9/2437 , A01H1/02 , C12N15/8249 , C12Y302/01004
摘要： The present invention provides a method for reducing fruit softening and cell wall polysaccharide degradation by inhibiting endo-1,4-.beta.-glucanase activity using antisense DNA constructions.
摘要翻译：本发明提供了一种通过使用反义DNA构建抑制内-4,4-β-葡聚糖酶活性来减少果实软化和细胞壁多糖降解的方法。

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