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    • 5. 发明授权
    • Sucrose accumulating tomato cultivar
    • 蔗糖积累番茄品种
    • US6072106A
    • 2000-06-06
    • US396408
    • 1995-02-28
    • Alan B. BennettSerge Yelle
    • Alan B. BennettSerge Yelle
    • A01H1/02A01H5/08A01H5/10A01H1/04A01H5/00
    • A01H5/10A01H1/02A01H5/08
    • A new tomato cultivar is provided which is homozygous for a genetically fixable recessive genetic factor which confers the ability to bear fruit that accumulate sucrose. The increased sucrose content, in turn, leads to a higher total soluble solids content in the mature fruit. Higher total soluble solids in tomato fruit are of particular importance to the tomato processing industry. A method for producing the claimed cultivar is also provided.The claimed genetic factor can be derived from any Lycopersicon species which accumulates sucrose in the mature fruit. Once genetically fixed in a tomato cultivar, the factor can be transmitted to other plants in a predictable manner.
    • 提供了一种新的番茄栽培品种,其为遗传上可固定的隐性遗传因子是纯合的,其赋予积累蔗糖的果实的能力。 增加的蔗糖含量又导致成熟果实中总可溶性固体含量较高。 番茄果实中较高的总可溶性固体对番茄加工业尤为重要。 还提供了生产所要求保护的品种的方法。 所要求的遗传因子可以来自在成熟果实中积累蔗糖的任何番茄种子。 一旦在番茄品种中遗传修复,该因子可以以可预测的方式传播给其他植物。
    • 8. 发明授权
    • Fructokinase genes and their use in metabolic engineering of fruit
sweetness
    • 果糖激酶基因及其在水果甜味代谢工程中的应用
    • US6031154A
    • 2000-02-29
    • US826611
    • 1997-04-05
    • Alan B. BennettYoshinori Kanayama
    • Alan B. BennettYoshinori Kanayama
    • C12N9/12C12N15/29C12N15/82A01H5/00C12N15/54C12P19/04
    • C12N9/1205C12N15/8245
    • This invention provides for novel cDNA and regulatory DNA sequences which modify carbohydrate metabolism in ripening fruit. In order to suppress fructokinase gene expression in transgenic plants and their fruit, we have isolated the cDNA of two tomato fructokinase genes that are expressed in developing and ripening fruit. These cDNA sequences, antisense sequences or ribozymes can be used to suppress expression of the transgenic plant's endogenous genes in ripening fruit by the use of chimeric constructions of the cDNA or antisense using either constitutive or fruit-specific and ripening-regulating regulatory sequences.In addition to a novel method of modifying carbohydrate metabolism, we have also found a novel fructokinase gene. Of the two tomato fructokinase genes described in this invention, one is highly homologous to previously described potato fructokinase gene; the other is highly divergent and represents a kinetically unique plant fructokinase.
    • 本发明提供了修饰成熟果实中碳水化合物代谢的新型cDNA和调控DNA序列。 为了抑制转基因植物及其果实中的果糖激酶基因表达,我们分离了在发育和成熟果实中表达的两种番茄果糖激酶基因的cDNA。 这些cDNA序列,反义序列或核酶可用于通过使用组成型或水果特异性和成熟调节调节序列的cDNA或反义体的嵌合构建来抑制转基因植物在成熟果实中的内源基因的表达。 除了一种新的碳水化合物代谢修饰方法外,我们还发现了一种新的果糖激酶基因。 在本发明描述的两种番茄果糖激酶基因中,一种与先前描述的马铃薯果糖激酶基因高度同源; 另一种是高度分歧的,代表动力学独特的植物果糖激酶。
    • 9. 发明授权
    • Fruit-specific and ripening-regulation expansin gene to control fruit texture and softening
    • 水果特异性和成熟调控扩增蛋白基因,以控制果实的质地和软化
    • US06350935B1
    • 2002-02-26
    • US09362642
    • 1999-07-27
    • Alan B. BennettJocelyn K. C. Rose
    • Alan B. BennettJocelyn K. C. Rose
    • C12N504
    • C12N15/8261C07K14/415C12N15/8249Y02A40/146
    • Expansins are proteins that induce extension in isolated plant cell walls in vitro and have been proposed to disrupt non-covalent interactions between hemicellulose and cellulose microfibrils. Because the plant primary cell wall acts as a constraint to cell enlargement, this process may be integral to plant cell expansion and studies of expansins have focused on their role in growth. We have discovered an expansin (Ex1) from tomato, melon and strawberry that is highly abundant and specifically expressed in ripening fruit, a developmental period when growth has ceased but when selective disassembly of cell wall components is pronounced. Also disclosed are expression vectors containing the Ex1 coding sequence, expression vectors containing an Ex1 sequence in the antisense orientation, Ex1 proteins, and transgenic plants which express both sense and antisense exogenous Ex1.
    • 扩展蛋白是在体外诱导分离的植物细胞壁中延伸的蛋白质,并且已被提议破坏半纤维素和纤维素微原纤维之间的非共价相互作用。 因为植物原发细胞壁是细胞扩大的一个制约因素,这个过程可能是植物细胞扩增的一个组成部分,而扩展蛋白的研究集中在它们在生长中的作用。 我们已经发现番茄,甜瓜和草莓中的膨化素(Ex1),其在成熟果实中是高度丰富和特异性表达的,发育期已经停止,但是细胞壁组分的选择性分解是显着的。 还公开了含有Ex1编码序列的表达载体,含有反义方向的Ex1序列的表达载体,Ex1蛋白和表达正义和反义外源性Ex1的转基因植物。
    • 10. 发明授权
    • Sucrose accumulating tomato technology
    • 蔗糖积累番茄技术
    • US5434344A
    • 1995-07-18
    • US509673
    • 1990-04-16
    • Alan B. BennettSerge Yelle
    • Alan B. BennettSerge Yelle
    • A01H1/02A01H5/08A01H5/10A01H1/00A01H5/00
    • A01H5/10A01H1/02A01H5/08
    • A new tomato cultivar is provided which is homozygous for a genetically fixable recessive genetic factor which confers the ability to bear fruit that accumulate sucrose. The increased sucrose content, in turn, leads to a higher total soluble solids content in the mature fruit. Higher total soluble solids in tomato fruit are of particular importance to the tomato processing industry. A method for producing the claimed cultivar is also provided.The claimed genetic factor can be derived from any Lycopersicon species which accumulates sucrose in the mature fruit. Once genetically fixed in a tomato cultivar, the factor can be transmitted to other plants in a predictable manner.
    • 提供了一种新的番茄栽培品种,其为遗传上可固定的隐性遗传因子是纯合的,其赋予积累蔗糖的果实的能力。 增加的蔗糖含量又导致成熟果实中总可溶性固体含量较高。 番茄果实中较高的总可溶性固体对番茄加工业尤为重要。 还提供了生产所要求保护的品种的方法。 所要求的遗传因子可以来自在成熟果实中积累蔗糖的任何番茄种子。 一旦在番茄品种中遗传修复,该因子可以以可预测的方式传播给其他植物。