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    • 7. 发明申请
    • METHOD FOR DETERMINING HEMATOCRIT CORRECTED ANALYTE CONCENTRATIONS
    • 用于确定HEMATOCRIT校正分析物浓度的方法
    • WO2008040982A1
    • 2008-04-10
    • PCT/GB2007/003770
    • 2007-10-05
    • LIFESCAN SCOTLAND LIMITEDBLYTHE, Stephen, PatrickCARDOSI, Marco, F.GILL, AndrewMILLS, LeanneLEACH, Christopher, Philip
    • BLYTHE, Stephen, PatrickCARDOSI, Marco, F.GILL, AndrewMILLS, LeanneLEACH, Christopher, Philip
    • G01N33/50G01N27/32
    • C12Q1/006G01N27/3271
    • Description is provided herein for an embodiment of a method determining a hematocrit-corrected glucose concentration. The exemplary method includes providing a test strip having a reference electrode and a working electrode, wherein the working electrode includes a plurality of microelectrodes and is coated with at least an enzyme and a mediator. The method can be achieved by: providing a test strip comprising a reference electrode and a working electrode formed with a plurality of microelectrodes and coated with a reagent layer; applying a fluid sample to the test strip for a reaction period; applying a test voltage between the reference electrode and the working electrode; measuring a test current as a function of time; measuring a steady state current value when the test current has reached an equilibrium; calculating a ratio of the test current to the steady state current value; plotting the ratio of the test current to the steady state current value as a function of the inverse square root of time; calculating an effective diffusion coefficient from the slope of the linearly regressed plot of the ratio of the test current to the steady state current value as a function of the inverse square root of time; and calculating a hematocrit-corrected concentration of analyte.
    • 本文提供了确定血细胞比容校正葡萄糖浓度的方法的实施方案的描述。 该示例性方法包括提供具有参考电极和工作电极的测试条,其中工作电极包括多个微电极并且至少涂覆有酶和介体。 该方法可以通过以下方式实现:提供包括参考电极和形成有多个微电极并涂覆有试剂层的工作电极的测试条; 将流体样品施加到测试条上一段反应期; 在参考电极和工作电极之间施加测试电压; 测量作为时间的函数的测试电流; 当测试电流达到平衡时测量稳态电流值; 计算测试电流与稳态电流值的比值; 将测试电流与稳态电流值的比率绘制为时间的逆平方根的函数; 根据测试电流与稳态电流值的比值的线性回归曲线的斜率计算有效扩散系数作为时间的平方根的函数; 并计算分析物的血细胞比容校正浓度。