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1. 发明申请

WO02097080A2 AMPLIFICATION VECTORS BASED ON TRANS-SPLICING 审中-公开
标题翻译：基于传输分离的放大矢量
公开(公告)号：WO02097080A2
公开(公告)日：2002-12-05
申请号：PCT/EP0204764
申请日：2002-04-30
申请人： ICON GENETICS AG , KLIMYUK VICTOR , IVANOV PETER , MARILLONNET SYLVESTRE , ZENK MEINHART , GLEBA YURI
发明人： KLIMYUK VICTOR , IVANOV PETER , MARILLONNET SYLVESTRE , ZENK MEINHART , GLEBA YURI
IPC分类号： C12N15/09 , C12N15/63 , C12N15/67 , C12N15/82 , C12N15/00
CPC分类号： C12N15/63 , C12N15/67 , C12N15/8203 , C12N15/8216
摘要： This invention provides process of amplification and/or expression of a sequence of interest in a cell by providing for generating within said cell at least one amplicon by trans-splicing between an RNA sequence designed for being capable of trans-splicing and a target RNA, whereby said amplicon is capable of amplifying in said cell and capable of expressing a sequence of interest.
摘要翻译：本发明提供在细胞中扩增和/或表达感兴趣的序列的方法，其通过在所述细胞内产生至少一个扩增子，通过在设计用于转接的RNA序列与靶RNA之间进行反式剪接，由此所述扩增子能够在所述细胞中扩增并且能够表达感兴趣的序列。

2. 发明专利

AT415479T 未知
公开(公告)号：AT415479T
公开(公告)日：2008-12-15
申请号：AT01940547
申请日：2001-06-01
申请人： BACHER ADELBERT , ZENK MEINHART H
发明人： BACHER ADELBERT , ZENK MEINHART , ADAM PETRA , EISENREICH WOLFGANG , FELLERMEIER MONIKA , HECHT STEFAN , ROHDICH FELIX , SCHUHR CHRISTOPH , WUNGSINTAWEEKUL JURAITHIP
IPC分类号： C12N15/52 , A01H5/10 , C12N9/00 , C12N15/82 , C12P9/00
摘要： Intermediate (A), optionally isotopically labeled, in the mevalonate-independent pathway of isoprenoid biosynthesis, is new. Independent claims are also included for the following: (a) aqueous cell fluid containing (I) and (A), with at least one present at higher concentration than in fluids from normal cells or organisms; (b) preparing (A); (c) enzymes (B) that convert (I) to (A); (d) isolated, purified nucleic acid (C) encoding (B); (e) DNA vectors containing (C); (f) recombinant cells containing the vector of (e); (g) screening chemical libraries for inhibitors of isoprenoid biosynthesis based on blocking conversion of (I); (h) identifying inhibitor-resistant variants of (B); (i) (B) variants identified by method (h); (j) nucleic acid (C') encoding the variants of (f); (k) (l) vectors containing (C'); (l) cells containing the vectors of (l); (m) imparting herbicide resistance to plants by incorporation of (C'); (n) weed control that involves growing crop plants of (n); (o) inhibitors (D) identified by method (g); (p) composition for inhibiting biosynthesis of isoprenoids containing (D); and (q) inhibiting biosynthesis of isoprenoids by treatment with (D) or the compositions of (q).

3. 发明专利

AU6280300A EISENREICH WOLFGANG 未知
公开(公告)号：AU6280300A
公开(公告)日：2001-03-05
申请号：AU6280300
申请日：2000-08-03
申请人： BACHER ADELBERT , ZENK MEINHART
发明人： BACHER ADELBERT , ZENK MEINHART , EISENREICH WOLFGANG , FELLERMEIER MONIKA , FISCHER MARKUS , HECHT STEFAN , HERZ STEFAN , KIS KLAUS , LUTTGEN HOLGER , ROHDICH FELIX , SAGNER SILVIA , SCHUHR CHRISTOPH A , WUNGSINTAWEEKUL JURAITHIP
IPC分类号： C12N9/12 , C12N9/88 , C12N15/54 , C12N15/60 , C12P23/00 , A01N61/00 , G01N33/68

4. 发明专利

DE19948616A1 Screening for inhibitors of the terpenoid biosynthetic pathway that may be used as herbicides 未知
公开(公告)号：DE19948616A1
公开(公告)日：2001-04-12
申请号：DE19948616
申请日：1999-10-08
申请人： BACHER ADELBERT , ZENK MEINHART
发明人： BACHER ADELBERT , ZENK MEINHART , FELLERMEIER MONIKA , KIS KLAUS
IPC分类号： C07H3/02 , C07H13/00 , C12Q1/02 , G01N33/50 , G01N33/92 , C07H11/04 , C07F9/09 , C12N9/99
摘要： A method (I) of screening for inhibitors of enzymes in the biosynthetic pathway that produces terpenoids via 1-deoxy-D-xylulose 5-phosphate in plants, comprises incubating plastids in the presence of a labeled precursor, ATP, CTP (or a source of CTP) and a candidate inhibitor and detecting the formation of labeled products, is new. Independent claims are included for the following: (1) a method (I) for screening for the presence of inhibition of at least one enzyme in the biosynthetic pathway to terpenoids via 1-deoxy-D-xylulose 5-phosphate in plants, comprising: (a) preparing a suspension of cells or plastids of a plastid-bearing organism in a culture medium for supporting the metabolism of the cells or plastids at least to the extent of the biosynthetic pathway; (b) adding to the suspension a predetermined amount of a carbon-13-, carbon-14-, deuterium-, or tritium-labeled biochemical precursor for generating terpenoids via the pathway; (c) incubating the mixture obtained in step (b) for a predetermined period of time at a predetermined temperature; (d) separating from the incubated mixture obtained in step (c), a fraction comprising a product or intermediate downstream from 1-deoxy-D-xylulose-5-phosphate in the pathway; (e) detecting the concentration of labeled product(s) in the fraction obtained in step (d); (f) repeating step (b) with the addition of a predetermined amount of a chemical test sample under otherwise identical conditions; (g) repeating steps (c) to (e) with the mixture obtained in step (f) under the same conditions as in steps (c) to (e); and (h) determining the presence of inhibition of at least one enzyme in the pathway by observation of whether the concentration of labeled product(s) detected in step (e) is higher than that detected when step (e) is repeated in step (g); (2) a method (II) for inhibiting the growth of a plant by treatment with a herbicide selected from a class of compounds exhibiting inhibition of the biosynthetic pathway to terpenoids via 1-deoxy-D-xylulose 5 phosphate in the test (I); (3) a method (III) of inhibiting, in the chloroplasts of plants, an enzyme in the biosynthetic pathway to terpenoids via 1-deoxy-D-xylulose 5 phosphate by treatment with a compound that inhibits that biosynthetic pathway identified in the test (I); (4) an inhibitor (IV) exhibiting inhibition in (I); (5) a method (V) for increasing the titer of an intermediate in the biosynthetic pathway via 1-deoxy-D-xylulose 5 phosphate upstream from IPP (undefined) in plants, plant cells or plastids by treatment with a synthetic inhibitor exhibiting inhibition in (I); (6) plant cells or plasmids containing a synthetic inhibitor and exhibiting a titer of an intermediate in the biosynthetic terpenoid pathway via 1-deoxy-D-xylulose 5 phosphate upstream from IPP which is increased over the titer absent in the inhibitor; (7) 14-carbon-, 13-carbon-, deuterium- or tritium-labelled 1-deoxy-D-xylulose 5 phosphate or 2C-methyl-D-erythritol 4-phosphate; (8) tritiated 2C-methyl-D-erythritol 4-phosphate or tritiated 1-deoxy-xylulose 5-phosphate; (9) 2C--methyl-D-erythritol 4-phosphate tritiated at positions 1 and/or 3 or in the methyl group; and (10) 1-deoxy-D-xylulose 5-phosphate, tritiated in positions 1, 3 and/or 4.

5. 发明专利

DE19948615A1 Screening for inhibitors of the terpenoid biosynthetic pathway that may be used as herbicides 未知
公开(公告)号：DE19948615A1
公开(公告)日：2001-04-12
申请号：DE19948615
申请日：1999-10-08
申请人： BACHER ADELBERT , ZENK MEINHART H
发明人： BACHER ADELBERT , ZENK MEINHART , FELLERMEIER MONIKA , KIS KLAUS
IPC分类号： C07H3/02 , C07H13/00 , C12Q1/02 , G01N33/50 , G01N33/92 , C07H11/04 , A01H3/04 , C12N5/04 , C12N9/99 , C12Q1/25
摘要： A method (I) of screening for inhibitors of enzymes in the biosynthetic pathway that produces terpenoids via 1-deoxy-D-xylulose 5-phosphate in plants, comprises incubating plastids in the presence of a labeled precursor, ATP, CTP (or a source of CTP) and a candidate inhibitor and detecting the formation of labeled products, is new. Independent claims are included for the following: (1) a method (I) for screening for the presence of inhibition of at least one enzyme in the biosynthetic pathway to terpenoids via 1-deoxy-D-xylulose 5-phosphate in plants, comprising: (a) preparing a suspension of cells or plastids of a plastid-bearing organism in a culture medium for supporting the metabolism of the cells or plastids at least to the extent of the biosynthetic pathway; (b) adding to the suspension a predetermined amount of a carbon-13-, carbon-14-, deuterium-, or tritium-labeled biochemical precursor for generating terpenoids via the pathway; (c) incubating the mixture obtained in step (b) for a predetermined period of time at a predetermined temperature; (d) separating from the incubated mixture obtained in step (c), a fraction comprising a product or intermediate downstream from 1-deoxy-D-xylulose-5-phosphate in the pathway; (e) detecting the concentration of labeled product(s) in the fraction obtained in step (d); (f) repeating step (b) with the addition of a predetermined amount of a chemical test sample under otherwise identical conditions; (g) repeating steps (c) to (e) with the mixture obtained in step (f) under the same conditions as in steps (c) to (e); and (h) determining the presence of inhibition of at least one enzyme in the pathway by observation of whether the concentration of labeled product(s) detected in step (e) is higher than that detected when step (e) is repeated in step (g); (2) a method (II) for inhibiting the growth of a plant by treatment with a herbicide selected from a class of compounds exhibiting inhibition of the biosynthetic pathway to terpenoids via 1-deoxy-D-xylulose 5 phosphate in the test (I); (3) a method (III) of inhibiting, in the chloroplasts of plants, an enzyme in the biosynthetic pathway to terpenoids via 1-deoxy-D-xylulose 5 phosphate by treatment with a compound that inhibits that biosynthetic pathway identified in the test (I); (4) an inhibitor (IV) exhibiting inhibition in (I); (5) a method (V) for increasing the titer of an intermediate in the biosynthetic pathway via 1-deoxy-D-xylulose 5 phosphate upstream from IPP (undefined) in plants, plant cells or plastids by treatment with a synthetic inhibitor exhibiting inhibition in (I); (6) plant cells or plasmids containing a synthetic inhibitor and exhibiting a titer of an intermediate in the biosynthetic terpenoid pathway via 1-deoxy-D-xylulose 5 phosphate upstream from IPP which is increased over the titer absent in the inhibitor; (7) 14-carbon-, 13-carbon-, deuterium- or tritium-labelled 1-deoxy-D-xylulose 5 phosphate or 2C-methyl-D-erythritol 4-phosphate; (8) tritiated 2C-methyl-D-erythritol 4-phosphate or tritiated 1-deoxy-xylulose 5-phosphate; (9) 2C--methyl-D-erythritol 4-phosphate tritiated at positions 1 and/or 3 or in the methyl group; and (10) 1-deoxy-D-xylulose 5-phosphate, tritiated in positions 1, 3 and/or 4.

6. 发明专利

DE10020996A1 未知
公开(公告)号：DE10020996A1
公开(公告)日：2001-03-22
申请号：DE10020996
申请日：2000-04-28
申请人： BACHER ADELBERT , ZENK MEINHART
发明人： BACHER ADELBERT , EISENREICH WOLFGANG , FELLERMEIER MONIKA , FISCHER MARKUS , HECHT STEFAN , HERZ STEFAN , KIS KLAUS , LUETTGEN HOLGER , ROHDICH FELIX , SAGNER SILVIA , SCHUHR CHRISTOPH A , WUNGSINTAWEEKUL JURAITHIP , ZENK MEINHART
IPC分类号： C12N9/12 , C12N9/88 , C12N15/54 , C12N15/60 , C12P23/00 , C07K14/245 , C07K14/415

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