专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

IL194479D0 PLANT VIRUS-BASED INDUCIBLE EXPRESSION SYSTEM 未知
公开(公告)号：IL194479D0
公开(公告)日：2011-08-01
申请号：IL19447908
申请日：2008-10-02
申请人： ICON GENETICS GMBH , WERNER STEFAN , MARILLONNET SYLVESTRE , KLIMYUK VICTOR , GLEBA YURI
IPC分类号： C12N20060101
摘要： A process of producing one or more than one protein of interest, comprising: (a) providing a plant or plant cells comprising a first heterologous nucleotide sequence comprising a nucleotide sequence encoding an RNA replicon, and a first inducible promoter operably linked to said nucleotide sequence encoding said RNA replicon; said RNA replicon not encoding a protein providing for cell-to-cell movement of said RNA replicon in said plant; said RNA replicon encoding a polymerase and said one or more than one protein of interest, said polymerase being adapted for replicating said RNA replicon; and (b) inducing, in said plant or plant cells of step (a), said inducible promoter, thereby producing said one or more than one protein of interest in said plant or plant cells.

2. 发明专利

IL173859A RNA VIRUS-DERIVED PLANT EXPRESSION SYSTEM 未知
公开(公告)号：IL173859A
公开(公告)日：2012-06-28
申请号：IL17385906
申请日：2006-02-21
申请人： ICON GENETICS GMBH , KLIMYUK VICTOR , GLEBA YURI , MARILLONNET SYLVESTRE , THOERINGER CAROLA
发明人： KLIMYUK VICTOR , GLEBA YURI , MARILLONNET SYLVESTRE , THOERINGER CAROLA
IPC分类号： C12N15/82

3. 发明专利

IL194479A PLANT VIRUS-BASED INDUCIBLE EXPRESSION SYSTEM 未知
公开(公告)号：IL194479A
公开(公告)日：2013-03-24
申请号：IL19447908
申请日：2008-10-02
申请人： ICON GENETICS GMBH , WERNER STEFAN , MARILLONNET SYLVESTRE , KLIMYUK VICTOR , GLEBA YURI
发明人： WERNER STEFAN , MARILLONNET SYLVESTRE , KLIMYUK VICTOR , GLEBA YURI
摘要： A process of producing one or more than one protein of interest, comprising: (a) providing a plant or plant cells comprising a first heterologous nucleotide sequence comprising a nucleotide sequence encoding an RNA replicon, and a first inducible promoter operably linked to said nucleotide sequence encoding said RNA replicon; said RNA replicon not encoding a protein providing for cell-to-cell movement of said RNA replicon in said plant; said RNA replicon encoding a polymerase and said one or more than one protein of interest, said polymerase being adapted for replicating said RNA replicon; and (b) inducing, in said plant or plant cells of step (a), said inducible promoter, thereby producing said one or more than one protein of interest in said plant or plant cells.

4. 发明申请

WO2006003018A3 BIOLOGICALLY SAFE TRANSIENT PROTEIN EXPRESSION IN PLANTS 审中-公开
标题翻译：植物中生物安全的瞬时蛋白表达
公开(公告)号：WO2006003018A3
公开(公告)日：2006-05-04
申请号：PCT/EP2005007361
申请日：2005-07-07
申请人： ICON GENETICS AG , MARILLONNET SYLVESTRE , ENGLER CAROLA , MUEHLBAUER STEFAN , HERZ STEFAN , WERNER STEFAN , KLIMYUK VICTOR , GLEBA YURI
发明人： MARILLONNET SYLVESTRE , ENGLER CAROLA , MUEHLBAUER STEFAN , HERZ STEFAN , WERNER STEFAN , KLIMYUK VICTOR , GLEBA YURI
IPC分类号： C12N15/82 , A01H5/12 , C12N15/74
CPC分类号： C12N15/8203 , C12N15/8205 , C12N15/8216 , C12N15/8257
摘要： A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterelogous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, b) optionally isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.
摘要翻译：通过在植物或植物叶中表达来自目的序列的所述感兴趣的蛋白质产生感兴趣的蛋白质的方法，包括：a）通过用农杆菌渗入所述植物或所述植物叶片来转染所述植物或所述植物叶子所述农杆菌菌株在T-DNA中含有具有编码复制子的序列部分的异源DNA序列，其中所述编码复制子的序列含有所述复制子复制子功能所必需的序列，所述序列源自植物病毒和所述目的序列从所述复制子表达，b）任选地从步骤（a）中渗入的所述植物或所述植物叶中分离所述感兴趣的蛋白质，其中所述土壤杆菌菌株具有第一遗传修饰呈递所述农杆菌菌株在不存在所述补体因子的情况下用所述T-DNA转染生物体是有缺陷的。

5. 发明申请

WO2006003018A2 BIOLOGICALLY SAFE TRANSIENT PROTEIN EXPRESSION IN PLANTS 审中-公开
标题翻译：生物安全瞬态蛋白表达在植物中
公开(公告)号：WO2006003018A2
公开(公告)日：2006-01-12
申请号：PCT/EP2005/007361
申请日：2005-07-07
申请人： ICON GENETICS AG , MARILLONNET, Sylvestre , ENGLER, Carola , MÜHLBAUER, Stefan , HERZ, Stefan , WERNER, Stefan , KLIMYUK, Victor , GLEBA, Yuri
发明人： MARILLONNET, Sylvestre , ENGLER, Carola , MÜHLBAUER, Stefan , HERZ, Stefan , WERNER, Stefan , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82 , A01H5/12 , C12N15/74
CPC分类号： C12N15/8203 , C12N15/8205 , C12N15/8216 , C12N15/8257
摘要： A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterelogous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, b) optionally isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.
摘要翻译：通过从植物或植物叶中感兴趣的序列表达所述感兴趣的蛋白质来产生感兴趣的蛋白质的方法，包括：a）通过用农杆菌浸润所述植物或所述植物叶来转染所述植物或所述植物叶子所述含有T-DNA的所述农杆菌菌株具有编码复制子的序列部分的恒耳DNA序列，其中所述编码复制子的序列含有所述复制子的复制子功能所需的序列，所述序列衍生自所述复制子表达的所述感兴趣的序列，b）任选地从所述植物或所述在步骤（a）中渗透的所述植物叶分离所述目的蛋白质，其中所述农杆菌菌株被提供第一遗传修饰呈现所述农杆菌菌株在没有所述补体因子的情况下缺陷用于用所述T-DNA转染生物体。

6. 发明申请

WO2005049839A3 RNA VIRUS-DERIVED PLANT EXPRESSION SYSTEM 审中-公开
标题翻译： RNA病毒衍生植物表达系统
公开(公告)号：WO2005049839A3
公开(公告)日：2005-11-03
申请号：PCT/EP2004012743
申请日：2004-11-10
申请人： ICON GENETICS AG , MARILLONNET SYLVESTRE , THOERINGER CAROLA , KLIMYUK VICTOR , GLEBA YURI
发明人： MARILLONNET SYLVESTRE , THOERINGER CAROLA , KLIMYUK VICTOR , GLEBA YURI
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8257 , C12N15/8203 , C12N15/8205 , C12N15/8216 , C12P21/00
摘要： A process of expressing a sequence of interest in a plant, plant part, or plant cell culture, comprising: (a) providing a plant, plant part, or plant cell culture containing in cell nuclei a heterologous DNA having a sequence encoding an RNA replicon operably linked or linkable to a transcription promoter, wherein said sequence encoding an RNA replicon contains (i) sequences for replicon function of said RNA replicon, said sequences being derived from a sequence of a plant RNA virus, (ii) a sequence of interest, whereby said sequences for replicon function exhibit at selected localities of said sequences of said plant RNA virus functionconservative differences from said sequence of said plant RNA virus, said differences causing an increased frequency of replicon formation compared to an RNA replicon not exhibiting said differences; and (b) causing expression of said sequence of interest.
摘要翻译：在植物，植物部分或植物细胞培养物中表达感兴趣的序列的方法，包括：（a）提供在细胞核中含有编码RNA复制子的序列的异源DNA的植物，植物部分或植物细胞培养物其可编码RNA复制子的所述序列包含（i）所述RNA复制子的复制子功能的序列，所述序列衍生自植物RNA病毒的序列，（ii）感兴趣的序列，其中所述用于复制子功能的序列在所述植物RNA病毒功能的所述序列的所选序列的所选位点处表现出与所述植物RNA病毒的所述序列相比的所述差异，所述差异导致复制子形成的频率增加而不显示所述差异的RNA复制子; 和（b）引起所述感兴趣序列的表达。

7. 发明申请

WO2004108934A1 SAFE PRODUCTION OF A PRODUCT OF INTEREST IN HYBRID SEEDS 审中-公开
标题翻译：安全生产混合种子利益产品
公开(公告)号：WO2004108934A1
公开(公告)日：2004-12-16
申请号：PCT/EP2004/006069
申请日：2004-06-04
申请人： ICON GENETICS AG , WERNER, Stefan , KANDZIA, Romy , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
发明人： WERNER, Stefan , KANDZIA, Romy , ELIBY, Serik , MARILLONNET, Sylvestre , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/8217 , C12N15/8242 , C12N15/8257
摘要： A process of the production of a product of interest in an F1 seed obtained by a hybridization of a first and a second transgenic parental plant, said hybridization generating a genetic endowment in said F1 seed for said production by combining in said F1 seed first and second partial genetic endowments of said first and second transgenic parental plants, followed by isolating said product of interest from said F1 seed or a seedling thereof.
摘要翻译：通过第一和第二转基因亲本植物的杂交获得的F1种子中感兴趣的产品的生产过程，所述杂交通过在所述F1种子中组合产生用于所述生产的遗传禀赋第一和第二所述第一和第二转基因亲本植物的部分遗传禀赋，然后从所述F1种子或其幼苗中分离所述目的产物。

8. 发明申请

WO2002077246A3 SITE-TARGETED TRANSFORMATION USING AMPLIFICATION VECTORS 审中-公开
公开(公告)号：WO2002077246A3
公开(公告)日：2002-10-03
申请号：PCT/EP2002/003266
申请日：2002-03-22
申请人： ICON GENETICS AG , ICON GENETICS, INC. , KLIMYUK, Victor , GLEBA, Yuri , MARILLONNET, Sylvestre
发明人： KLIMYUK, Victor , GLEBA, Yuri , MARILLONNET, Sylvestre
IPC分类号： C12N15/82
摘要： A process of causing a targeted integration of DNA of interest into a plynt cell nuclear genome, comprising; i) providing plant cells with an amplification vector, or a precursor thereof, capable of autonomous replication in plant cells, said vector comprising; a) DNA sequence(s) encoding an origin of replication functional in plant cells, b) DNA sequence(s) necessary for site-specific and/or homologous recombination between the vector and a host nuclear DNA, and c) optionally, further DNA of interest; ii) optionally providing conditions that facilitate vector amplification and/or cell to cell movement and/or site-specific and/or homologous reconbination, and iii) selecting cells having undergone recombination at a predetermined site in the plant nuclear DNA.

9. 发明申请

WO2007031339A1 PLANT VIRAL PARTICLES COMPRISING A PLURALITY OF FUSION PROTEINS CONSISTING OF A PLANT VIRAL COAT PROTEIN, A PEPTIDE LINKER AND A RECOMBINANT PROTEIN AND USE OF SUCH PLANT VIRAL PARTICLES FOR PROTEIN PURIFICATION 审中-公开
标题翻译：包含植物病毒蛋白质，肽链接器和重组蛋白质的多种融合蛋白的植物病毒颗粒以及用于蛋白质纯化的这种植物病毒颗粒的使用
公开(公告)号：WO2007031339A1
公开(公告)日：2007-03-22
申请号：PCT/EP2006/009029
申请日：2006-09-15
申请人： ICON GENETICS AG , WERNER, Stefan , MARILLONNET, Sylvestre , KLIMYUK, Viktor , GLEBA, Yuri
发明人： WERNER, Stefan , MARILLONNET, Sylvestre , KLIMYUK, Viktor , GLEBA, Yuri
IPC分类号： C12N15/82 , C07K14/08
CPC分类号： C12N15/8258 , C07K2319/705 , C12N7/00 , C12N15/8203 , C12N15/8257 , C12N2770/00043 , C12N2770/26023
摘要： A process of purifying a protein of interest using viral particles or virus-like particles comprising a plurality of fusion protein molecules, said fusion protein comprising the following fusion protein domains: (i) a plant viral coat protein, (ii) a recombinant protein, and (iii) optionally a peptide linker linking said plant viral coat protein and said recombinant protein, wherein formation of said viral particle does not require free viral coat protein.
摘要翻译：一种使用包含多个融合蛋白分子的病毒颗粒或病毒样颗粒纯化感兴趣的蛋白质的方法，所述融合蛋白包含以下融合蛋白结构域：（i）植物病毒外壳蛋白，（ii）重组蛋白，和（iii）任选地连接所述植物病毒外壳蛋白和所述重组蛋白的肽接头，其中所述病毒颗粒的形成不需要游离的病毒外壳蛋白。

10. 发明申请

WO2006012906A1 RNA VIRUS-DERIVED PLANT EXPRESSION SYSTEM 审中-公开
标题翻译： RNA病毒衍生植物表达系统
公开(公告)号：WO2006012906A1
公开(公告)日：2006-02-09
申请号：PCT/EP2003/012530
申请日：2003-11-10
申请人： ICON GENETICS AG , ICON GENETICS, INC. , MARILLONNET, Sylvestre , THOERINGER, Carola , KLIMYUK, Victor , GLEBA, Yuri
发明人： MARILLONNET, Sylvestre , THOERINGER, Carola , KLIMYUK, Victor , GLEBA, Yuri
IPC分类号： C12N15/82
CPC分类号： C12N15/8203
摘要： A stably transformed plant, plant part, or plant cell culture containing in cell nuclei a heterologous DNA having a sequence encoding an RNA replicon operably linked or linkable to a transcription promoter, wherein said sequence encoding an RNA replicon contains (i) sequences for replicon function of said RNA replicon, said sequences being derived from a sequence of a plant RNA virus, and (ii) a sequence of interest to be expressed from said RNA replicon, whereby said sequences for replicon function exhibit at selected localities of said sequence of said plant RNA virus function-conservative differences from said sequence of said plant RNA virus, said differences causing an increased frequency of replicon formation compared to an RNA replicon not exhibiting said differences.
摘要翻译：一种稳定转化的植物，植物部分或植物细胞培养物，其在细胞核中含有具有可操作地连接或可与转录启动子连接的RNA复制子的序列的异源DNA，其中编码RNA复制子的所述序列包含（i）复制子功能序列的所述RNA复制子，所述序列衍生自植物RNA病毒的序列，和（ii）将从所述RNA复制子表达的所需序列，其中所述复制子功能的序列在所述植物的所述序列的选定位置显示与所述植物RNA病毒的所述序列的RNA病毒功能保守差异，与不显示所述差异的RNA复制子相比，所述差异导致复制子形成的频率增加。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式