专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO02097080A2 AMPLIFICATION VECTORS BASED ON TRANS-SPLICING 审中-公开
标题翻译：基于传输分离的放大矢量
公开(公告)号：WO02097080A2
公开(公告)日：2002-12-05
申请号：PCT/EP0204764
申请日：2002-04-30
申请人： ICON GENETICS AG , KLIMYUK VICTOR , IVANOV PETER , MARILLONNET SYLVESTRE , ZENK MEINHART , GLEBA YURI
发明人： KLIMYUK VICTOR , IVANOV PETER , MARILLONNET SYLVESTRE , ZENK MEINHART , GLEBA YURI
IPC分类号： C12N15/09 , C12N15/63 , C12N15/67 , C12N15/82 , C12N15/00
CPC分类号： C12N15/63 , C12N15/67 , C12N15/8203 , C12N15/8216
摘要： This invention provides process of amplification and/or expression of a sequence of interest in a cell by providing for generating within said cell at least one amplicon by trans-splicing between an RNA sequence designed for being capable of trans-splicing and a target RNA, whereby said amplicon is capable of amplifying in said cell and capable of expressing a sequence of interest.
摘要翻译：本发明提供在细胞中扩增和/或表达感兴趣的序列的方法，其通过在所述细胞内产生至少一个扩增子，通过在设计用于转接的RNA序列与靶RNA之间进行反式剪接，由此所述扩增子能够在所述细胞中扩增并且能够表达感兴趣的序列。

2. 发明申请

WO0229068A2 VECTOR SYSTEM FOR PLANTS 审中-公开
标题翻译：植物植物系统
公开(公告)号：WO0229068A2
公开(公告)日：2002-04-11
申请号：PCT/EP0111629
申请日：2001-10-08
申请人： ICON GENETICS AG , GLEBA YURI , DOROKOV YURII , IVANOV PETER , ATABEKOV JOSEPH
发明人： GLEBA YURI , DOROKOV YURII , IVANOV PETER , ATABEKOV JOSEPH
IPC分类号： A01H5/00 , C12N5/10 , C12N15/09 , C12N15/12 , C12N15/29 , C12N15/82 , C12Q1/68 , C12N15/63
CPC分类号： C12N15/8216 , C12N15/8203 , C12N15/8257
摘要： The invention describes virus-based amplification vectors for plants containing additional plant-specific internal ribosome entry site (IRES) element(s) allowing for a polycistronic translation and a cap-independent translation of : a) heterologous gene(s); b) whole viral genome or c) viral subgenomic RNAs. Said IRES elements are of plant viral origin, or they are isolated from other organisms or engineered using different synthesis procedures. Said IRES element(s) and said heterologous gene(s) are inserted into ampification vectors and allow for the expression of said heterologous gene(s) in the absence of additional viral promoters, in particular, said expression is achieved through cap-independent translation.
摘要翻译：本发明描述了含有额外的植物特异性内部核糖体进入位点（IRES）元件的植物的基于病毒的扩增载体，其允许多顺反子翻译和与帽无关的翻译：a）异源基因; b）全病毒基因组或c）病毒亚基因组RNA。所述IRES元件具有植物病毒来源，或者它们与其他生物体分离或者使用不同的合成方法进行工程化。将所述IRES元件和所述异源基因插入到放大载体中，并允许在不存在另外的病毒启动子的情况下表达所述异源基因，特别地，所述表达是通过与帽无关的翻译。

3. 发明申请

WO03020927A3 CREATION OF ARTIFICIAL INTERNAL RIBOSOME ENTRY SITE (IRES) ELEMENTS 审中-公开
标题翻译：人造内部入口网站（IRES）元素的创建
公开(公告)号：WO03020927A3
公开(公告)日：2003-07-24
申请号：PCT/EP0209843
申请日：2002-09-03
申请人： ICON GENETICS INC , ATABEKOV JOSEPH , DOROKHOV YURII , SKULACHEV MAXIM , IVANOV PETER , GLEBA YURI
发明人： ATABEKOV JOSEPH , DOROKHOV YURII , SKULACHEV MAXIM , IVANOV PETER , GLEBA YURI
IPC分类号： A01H5/00 , C12N1/15 , C12N1/19 , C12N5/10 , C12N15/09 , C12N15/67 , C12N15/82 , C12Q1/02 , G06F17/30 , C12N15/11 , C12N15/81 , C12N15/85 , C12Q1/68
CPC分类号： C12N15/8216 , C12N15/67
摘要： A method of creating a nucleic acid sequence having an adenine-rich nucleic acid block of at least 25 nucleotides in length is provided, whereby said nucleic acid sequence is capable of causing translation by internal ribosome entry (IRES element). Said adenine-rich nucleic acid block comprises from 40 to 100 mol-% adenine.
摘要翻译：提供了具有长度为至少25个核苷酸的富含腺嘌呤核酸嵌段的核酸序列的方法，由此所述核酸序列能够通过内部核糖体进入（IRES元件）引起翻译。所述富含腺嘌呤的核酸嵌段包含40-100mol-腺嘌呤。

4. 发明申请

WO03020928A2 IDENTIFICATION OF EUKARYOTIC INTERNAL RIBOSOME ENTRY SITE (IRES) ELEMENTS 审中-公开
标题翻译：真核内部RIBOSOME入口点（IRES）元件的鉴定
公开(公告)号：WO03020928A2
公开(公告)日：2003-03-13
申请号：PCT/EP0209844
申请日：2002-09-03
申请人： ICON GENETICS INC , ATABEKOV JOSEPH , DOROKHOV YURII , SKULACHEV MAXIM , IVANOV PETER , GLEBA YURI
发明人： ATABEKOV JOSEPH , DOROKHOV YURII , SKULACHEV MAXIM , IVANOV PETER , GLEBA YURI
IPC分类号： C12N15/09 , A01H5/00 , A01K67/027 , C12N1/15 , C12N1/19 , C12N5/10 , C12N15/10 , C12N15/67 , C12N15/82 , C12Q1/68 , G06F19/00 , C12N15/81 , C12N15/85 , G06F17/00
CPC分类号： C12N15/8216 , C12N15/1051 , C12N15/67 , C12N15/8273 , C12N15/8279 , C12N2510/00
摘要： A method of search for and identification of a eukaryotic IRES element active in cap-independent translation of RNA in eukaryiotic cells is provided, comprising the following steps: i)screening eukaryotic mRNA sequences or corresponding DNA sequences for a potential IRES element having a block of nucleotides having: a) a length of at least 30 nucleotides; b) an adenine nucleotide content of at least 40 mol-%; and c) a pyrimidine nucleotide content of less than 40 mol-%; ii) inserting said potential IRES element into a linear dicistronic construct between an upstream gene and a downstream GUS reporter gene, whereby said potential IRES element is positioned for IRES-dependent translation of said downstream GUS gene and whereby said upstream gene is preceded by a stable hairpin structure to prevent IRES-independent translation of said genes; and iii) testing said potential IRES element for IRES-dependent translation of said GUS gene in a rabbit reticulocyte lysate or in a wheat germ extract in vitro translation assay, whereby GUS gene expression is quantitated preferably relative to a construct having a reference IRES element or a non-IRES element between said upstream gene and said GUS gene.
摘要翻译：提供了一种在真核生物细胞中寻找和鉴定在帽子非依赖性翻译中活性的真核IRES元件的方法，其包括以下步骤：i）筛选具有嵌段的潜在IRES元件的真核mRNA序列或相应的DNA序列核苷酸具有：a）至少30个核苷酸的长度; b）至少40mol-％的腺嘌呤核苷酸含量; 和c）小于40摩尔％的嘧啶核苷酸含量; ii）将所述潜在IRES元件插入到上游基因和下游GUS报道基因之间的线性双顺反子构建体中，由此所述潜在IRES元件被定位用于所述下游GUS基因的IRES依赖性翻译，并且其中所述上游基因之前是稳定的发夹结构以防止所述基因的IRES非依赖性翻译; 和iii）在兔网织红细胞裂解物或小麦胚芽提取物体外翻译测定中测试所述潜在IRES元件对于所述GUS基因的IRES依赖性翻译，由此GUS基因表达优选相对于具有参考IRES元件的构建体或所述上游基因和所述GUS基因之间的非IRES元件。

5. 发明专利

CA2421306A1 VIRUS-BASED AMPLIFICATION VECTOR FOR PLANTS 未知
公开(公告)号：CA2421306A1
公开(公告)日：2002-04-11
申请号：CA2421306
申请日：2001-10-08
申请人： ICON GENETICS AG
发明人： GLEBA YURI , ATABEKOV JOSEPH , IVANOV PETER , DOROKOV YURII
IPC分类号： A01H5/00 , C12N5/10 , C12N15/09 , C12N15/12 , C12N15/29 , C12N15/82 , C12Q1/68 , C12N15/83 , C12N15/63
摘要： The invention describes virus-based amplification vectors for plants containing additional plant-specific internal ribosome entry site (IRES) element(s) allowing for a polycistronic translation and a cap-independent translation of : a) heterologous gene(s); b) whole viral genome or c) viral subgenomic RNAs. Said IRES elements are of plant viral origin, or they are isolated from other organisms or engineered using different synthesis procedures. Said IRES element(s) and said heterologous gene(s) are inserted into ampification vectors and allow for the expression of said heterologous gene(s) in the absence of additional viral promoters, in particular, said expression is achieved through cap-independent translation.

6. 发明专利

AU1400302A Vector system for plants 未知
公开(公告)号：AU1400302A
公开(公告)日：2002-04-15
申请号：AU1400302
申请日：2001-10-08
申请人： ICON GENETICS AG
发明人： GLEBA YURI , DOROKOV YURII , IVANOV PETER , ATABEKOV JOSEPH
IPC分类号： A01H5/00 , C12N5/10 , C12N15/09 , C12N15/12 , C12N15/29 , C12N15/82 , C12Q1/68 , C12N15/63
摘要： The invention describes virus-based amplification vectors for plants containing additional plant-specific internal ribosome entry site (IRES) element(s) allowing for a polycistronic translation and a cap-independent translation of: a) heterologous gene(s); b) whole viral genome or c) viral subgenomic RNAs. Said IRES elements are of plant viral origin, or they are isolated from other organisms or engineered using different synthesis procedures. Said IRES element(s) and said heterologous gene(s) are inserted into amplification vectors and allow for the expression of said heterologous gene(s) in the absence of additional viral promoters, in particular, said expression is achieved through cap-independent translation.

7. 发明专利

DE10143237A1 未知
公开(公告)号：DE10143237A1
公开(公告)日：2003-03-20
申请号：DE10143237
申请日：2001-09-04
申请人： ICON GENETICS AG
发明人： ATABEKOV JOSEPH , DOROKHOV YURIJ , SKULACHEV MAXIM , IVANOV PETER , GLEBA YURI
IPC分类号： A01H5/00 , C12N1/15 , C12N1/19 , C12N5/10 , C12N15/09 , C12N15/67 , C12N15/82 , C12Q1/02 , G06F17/30 , C12N15/10
摘要： A method of creating a nucleic acid sequence having an adenine-rich nucleic acid block of at least 25 nucleotides in length is provided, whereby said nucleic acid sequence is capable of causing translation by internal ribosome entry (IRES element). Said adenine-rich nucleic acid block comprises from 40 to 100 mol-% adenine.

8. 发明专利

DE10143238A1 未知
公开(公告)号：DE10143238A1
公开(公告)日：2003-03-20
申请号：DE10143238
申请日：2001-09-04
申请人： ICON GENETICS AG
发明人： ATABEKOV JOSEF , DOROKHOV YURIJ , SKULACHEV MAXIM , IVANOV PETER , GLEBA YURI
IPC分类号： C12N15/09 , A01H5/00 , A01K67/027 , C12N1/15 , C12N1/19 , C12N5/10 , C12N15/10 , C12N15/67 , C12N15/82 , C12Q1/68 , G06F19/00
摘要： A method of search for and identification of a eukaryotic IRES element active in cap-independent translation of RNA in eukaryiotic cells is provided, comprising the following steps: i)screening eukaryotic mRNA sequences or corresponding DNA sequences for a potential IRES element having a block of nucleotides having: a) a length of at least 30 nucleotides; b) an adenine nucleotide content of at least 40 mol-%; and c) a pyrimidine nucleotide content of less than 40 mol-%; ii) inserting said potential IRES element into a linear dicistronic construct between an upstream gene and a downstream GUS reporter gene, whereby said potential IRES element is positioned for IRES-dependent translation of said downstream GUS gene and whereby said upstream gene is preceded by a stable hairpin structure to prevent IRES-independent translation of said genes; and iii) testing said potential IRES element for IRES-dependent translation of said GUS gene in a rabbit reticulocyte lysate or in a wheat germ extract in vitro translation assay, whereby GUS gene expression is quantitated preferably relative to a construct having a reference IRES element or a non-IRES element between said upstream gene and said GUS gene.

9. 发明专利

DE10049587A1 未知
公开(公告)号：DE10049587A1
公开(公告)日：2002-05-02
申请号：DE10049587
申请日：2000-10-06
申请人： ICON GENETICS AG
发明人： DOROKHOV YURII , IVANOV PETER , ATABEKOV JOSEPH , GLEBA YURI
IPC分类号： A01H5/00 , C12N5/10 , C12N15/09 , C12N15/12 , C12N15/29 , C12N15/82 , C12Q1/68
摘要： The invention describes virus-based amplification vectors for plants containing additional plant-specific internal ribosome entry site (IRES) element(s) allowing for a polycistronic translation and a cap-independent translation of: a) heterologous gene(s); b) whole viral genome or c) viral subgenomic RNAs. Said IRES elements are of plant viral origin, or they are isolated from other organisms or engineered using different synthesis procedures. Said IRES element(s) and said heterologous gene(s) are inserted into amplification vectors and allow for the expression of said heterologous gene(s) in the absence of additional viral promoters, in particular, said expression is achieved through cap-independent translation.

10. 发明专利

AU7533998A Methods for coexpression of more than one gene using at least one internal ribosome entry site (ires) 未知
公开(公告)号：AU7533998A
公开(公告)日：1998-12-30
申请号：AU7533998
申请日：1998-05-29
申请人： ATABEKOV JOSEPH , IVANOV PETER , SKULACHEV MAXIM , NINA RODIONOVA , OLGA KARPOVA , TIMO KORPELA , YURII DOROKHOV
发明人： ATABEKOV JOSEPH , KORPELA TIMO , DOROKHOV YURII , IVANOV PETER , SKULACHEV MAXIM , RODIONOVA NINA , KARPOVA OLGA
IPC分类号： A01H5/00 , C12N1/15 , C12N1/19 , C12N5/10 , C12N15/09 , C12N15/82 , C12R1/94 , C12N15/83
摘要： The present invention is drawn to a recombinant nucleic acid having (a) a transcriptional promoter; (b) a first plant-expressible structural gene linked to the transcriptional promoter, (c) a cDNA sequence element having an internal ribosome entry site of tobamovirus moving protein (MP) gene (IRESmp) which is located 3' to the first plant-expressible structural gene, and (d) a second plant-expressible gene located 3' to the (IRESmp) such that the second gene is placed under the translational control of (IRESmp); wherein the first plant-expressible gene, (IRESmp) and the second plant-expressible gene are transcribed under the action of the transcriptional promoter to give a primary transcript, and the first plant expressible gene of the primary transcript is able to translate by ribosome scanning mechanism and the second plant expressible gene of the primary transcript is capable of translation under the action of (IRESMP). The present invention is further drawn to a method of simultaneously expressing desired genes in vitro and in planta by using crTMV RNA sequences upstream or the MP gene, i.e. IRESmp.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式